Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider

Autores
Izquierdo, Matías Andres; Dederichs, Tim M.; Cargnelutti, Franco Ignacio; Michalik, Peter
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The male genitalia of pholcid spiders, which is one of the most species-rich spider families, are characterized by a procursus, which is a morphologically diverse projection of thecopulatory organ. It has been shown that the procursus interacts with the female genitalia during copulation. Here, we investigate the function of the procursus in Gertschiola neuquena, a species belonging to the early branched and understudied subfamily Ninetinae, using behavioural andmorphological data. Although many aspects of the copulatory behaviour of G. neuquena follow the general pattern described for the family, males use only one pedipalp during each copulation. Based on our micro-CT analysis of cryofixed mating pairs using virgin females, we can show that the longand filiform procursus is inserted deeply into the unpaired convoluted female spermatheca, and the intromittent sclerite, the embolus, is rather short and stout only reaching the most distal part of the female sperm storage organ. Histological data revealed that sperm are present in the most proximalpart of the spermatheca, suggesting that the procursus is used to allocate sperm deeply into the female sperm storage organ. This represents the first case of a replacement of the spermallocation function of the intromittent sclerite in spiders.
Fil: Izquierdo, Matías Andres. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Dederichs, Tim M.. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania
Fil: Cargnelutti, Franco Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Michalik, Peter. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania
Materia
THREE DIMENSIONAL RECONSTRUCTION
SPIDER GENITALIA
SPIDER BEHAVIOUR
COPULATORY MECHANICS
SPERM TRANSFER
ARANEAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229613
Acceder
id CONICETDig_8370a49da1a5781cb9734ffba780cf66
oai_identifier_str oai:ri.conicet.gov.ar:11336/229613
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spiderIzquierdo, Matías AndresDederichs, Tim M.Cargnelutti, Franco IgnacioMichalik, PeterTHREE DIMENSIONAL RECONSTRUCTIONSPIDER GENITALIASPIDER BEHAVIOURCOPULATORY MECHANICSSPERM TRANSFERARANEAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The male genitalia of pholcid spiders, which is one of the most species-rich spider families, are characterized by a procursus, which is a morphologically diverse projection of thecopulatory organ. It has been shown that the procursus interacts with the female genitalia during copulation. Here, we investigate the function of the procursus in Gertschiola neuquena, a species belonging to the early branched and understudied subfamily Ninetinae, using behavioural andmorphological data. Although many aspects of the copulatory behaviour of G. neuquena follow the general pattern described for the family, males use only one pedipalp during each copulation. Based on our micro-CT analysis of cryofixed mating pairs using virgin females, we can show that the longand filiform procursus is inserted deeply into the unpaired convoluted female spermatheca, and the intromittent sclerite, the embolus, is rather short and stout only reaching the most distal part of the female sperm storage organ. Histological data revealed that sperm are present in the most proximalpart of the spermatheca, suggesting that the procursus is used to allocate sperm deeply into the female sperm storage organ. This represents the first case of a replacement of the spermallocation function of the intromittent sclerite in spiders.Fil: Izquierdo, Matías Andres. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Dederichs, Tim M.. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; AlemaniaFil: Cargnelutti, Franco Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Michalik, Peter. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; AlemaniaThe Royal Society2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229613Izquierdo, Matías Andres; Dederichs, Tim M.; Cargnelutti, Franco Ignacio; Michalik, Peter; Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider; The Royal Society; Royal Society Open Sience; 10; 5; 5-2023; 1-142054-5703CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://royalsocietypublishing.org/doi/10.1098/rsos.230263info:eu-repo/semantics/altIdentifier/doi/10.1098/rsos.230263info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:44Zoai:ri.conicet.gov.ar:11336/229613instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:44.651CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider
title Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider
spellingShingle Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider
Izquierdo, Matías Andres
THREE DIMENSIONAL RECONSTRUCTION
SPIDER GENITALIA
SPIDER BEHAVIOUR
COPULATORY MECHANICS
SPERM TRANSFER
ARANEAE
title_short Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider
title_full Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider
title_fullStr Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider
title_full_unstemmed Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider
title_sort Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider
dc.creator.none.fl_str_mv Izquierdo, Matías Andres
Dederichs, Tim M.
Cargnelutti, Franco Ignacio
Michalik, Peter
author Izquierdo, Matías Andres
author_facet Izquierdo, Matías Andres
Dederichs, Tim M.
Cargnelutti, Franco Ignacio
Michalik, Peter
author_role author
author2 Dederichs, Tim M.
Cargnelutti, Franco Ignacio
Michalik, Peter
author2_role author
author
author
dc.subject.none.fl_str_mv THREE DIMENSIONAL RECONSTRUCTION
SPIDER GENITALIA
SPIDER BEHAVIOUR
COPULATORY MECHANICS
SPERM TRANSFER
ARANEAE
topic THREE DIMENSIONAL RECONSTRUCTION
SPIDER GENITALIA
SPIDER BEHAVIOUR
COPULATORY MECHANICS
SPERM TRANSFER
ARANEAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The male genitalia of pholcid spiders, which is one of the most species-rich spider families, are characterized by a procursus, which is a morphologically diverse projection of thecopulatory organ. It has been shown that the procursus interacts with the female genitalia during copulation. Here, we investigate the function of the procursus in Gertschiola neuquena, a species belonging to the early branched and understudied subfamily Ninetinae, using behavioural andmorphological data. Although many aspects of the copulatory behaviour of G. neuquena follow the general pattern described for the family, males use only one pedipalp during each copulation. Based on our micro-CT analysis of cryofixed mating pairs using virgin females, we can show that the longand filiform procursus is inserted deeply into the unpaired convoluted female spermatheca, and the intromittent sclerite, the embolus, is rather short and stout only reaching the most distal part of the female sperm storage organ. Histological data revealed that sperm are present in the most proximalpart of the spermatheca, suggesting that the procursus is used to allocate sperm deeply into the female sperm storage organ. This represents the first case of a replacement of the spermallocation function of the intromittent sclerite in spiders.
Fil: Izquierdo, Matías Andres. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Dederichs, Tim M.. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania
Fil: Cargnelutti, Franco Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Michalik, Peter. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania
description The male genitalia of pholcid spiders, which is one of the most species-rich spider families, are characterized by a procursus, which is a morphologically diverse projection of thecopulatory organ. It has been shown that the procursus interacts with the female genitalia during copulation. Here, we investigate the function of the procursus in Gertschiola neuquena, a species belonging to the early branched and understudied subfamily Ninetinae, using behavioural andmorphological data. Although many aspects of the copulatory behaviour of G. neuquena follow the general pattern described for the family, males use only one pedipalp during each copulation. Based on our micro-CT analysis of cryofixed mating pairs using virgin females, we can show that the longand filiform procursus is inserted deeply into the unpaired convoluted female spermatheca, and the intromittent sclerite, the embolus, is rather short and stout only reaching the most distal part of the female sperm storage organ. Histological data revealed that sperm are present in the most proximalpart of the spermatheca, suggesting that the procursus is used to allocate sperm deeply into the female sperm storage organ. This represents the first case of a replacement of the spermallocation function of the intromittent sclerite in spiders.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229613
Izquierdo, Matías Andres; Dederichs, Tim M.; Cargnelutti, Franco Ignacio; Michalik, Peter; Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider; The Royal Society; Royal Society Open Sience; 10; 5; 5-2023; 1-14
2054-5703
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229613
identifier_str_mv Izquierdo, Matías Andres; Dederichs, Tim M.; Cargnelutti, Franco Ignacio; Michalik, Peter; Copulatory behaviour and genital mechanics suggest sperm allocation by a non-intromittent sclerite in a pholcid spider; The Royal Society; Royal Society Open Sience; 10; 5; 5-2023; 1-14
2054-5703
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://royalsocietypublishing.org/doi/10.1098/rsos.230263
info:eu-repo/semantics/altIdentifier/doi/10.1098/rsos.230263
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv The Royal Society
publisher.none.fl_str_mv The Royal Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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