ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds

Autores
Tognacca, Rocío Soledad; Monica, Carabelli; Giorgio, Morelli; Ida, Ruberti; Botto, Javier Francisco
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The germination timing of seeds is of the utmost adaptive importance for plant populations. Light is one of the best characterized factors promoting seed germination in several species. The germination is also fnely regulated by changes in hormones levels, mainly those of gibberellin (GA) and abscisic acid (ABA). Here, we performed physiological, pharmacological, and molecular analyses to uncover the role of ATHB2, an HD-ZIP II transcription factor, in germination of Arabidopsis seeds. Our study demonstrated that ATHB2 is a negative regulator and sustains the expression of transcription factors to block germination promoted by light. Besides, we found that ATHB2 increases ABA sensitivity. Moreover, ABA and auxin content in athb2-2 mutant is higher than wild-type in dry seeds, but the diferences disappeared during the imbibition in darkness and the frst hours of exposition to light, respectively. Some ABA and light transcription factors are up-regulated by ATHB2, such as ABI5, ABI3, XERICO, SOMNUS and PIL5/PIF1. In opposition, PIN7, an auxin transport, is down-regulated. The role of ATHB2 as a repressor of germination induced by light afecting the gemination timing, could have diferential efects on the establishment of seedlings altering the competitiveness between crops and weeds in the feld.
Fil: Tognacca, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Monica, Carabelli. Consiglio Nazionale delle Ricerche; Italia
Fil: Giorgio, Morelli. Research Centre for Genomics and Bioinformatics, Council for Agricultural Research and Economics; Italia
Fil: Ida, Ruberti. Consiglio Nazionale delle Ricerche; Italia
Fil: Botto, Javier Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Materia
germination
seeds
ATHB2
Light
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/168111
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/168111
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling ATHB2 is a negative regulator of germination in Arabidopsis thaliana seedsTognacca, Rocío SoledadMonica, CarabelliGiorgio, MorelliIda, RubertiBotto, Javier FranciscogerminationseedsATHB2Lighthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The germination timing of seeds is of the utmost adaptive importance for plant populations. Light is one of the best characterized factors promoting seed germination in several species. The germination is also fnely regulated by changes in hormones levels, mainly those of gibberellin (GA) and abscisic acid (ABA). Here, we performed physiological, pharmacological, and molecular analyses to uncover the role of ATHB2, an HD-ZIP II transcription factor, in germination of Arabidopsis seeds. Our study demonstrated that ATHB2 is a negative regulator and sustains the expression of transcription factors to block germination promoted by light. Besides, we found that ATHB2 increases ABA sensitivity. Moreover, ABA and auxin content in athb2-2 mutant is higher than wild-type in dry seeds, but the diferences disappeared during the imbibition in darkness and the frst hours of exposition to light, respectively. Some ABA and light transcription factors are up-regulated by ATHB2, such as ABI5, ABI3, XERICO, SOMNUS and PIL5/PIF1. In opposition, PIN7, an auxin transport, is down-regulated. The role of ATHB2 as a repressor of germination induced by light afecting the gemination timing, could have diferential efects on the establishment of seedlings altering the competitiveness between crops and weeds in the feld.Fil: Tognacca, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Monica, Carabelli. Consiglio Nazionale delle Ricerche; ItaliaFil: Giorgio, Morelli. Research Centre for Genomics and Bioinformatics, Council for Agricultural Research and Economics; ItaliaFil: Ida, Ruberti. Consiglio Nazionale delle Ricerche; ItaliaFil: Botto, Javier Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaSpringer Nature2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/168111Tognacca, Rocío Soledad; Monica, Carabelli; Giorgio, Morelli; Ida, Ruberti; Botto, Javier Francisco; ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds; Springer Nature; Scientific Reports; 11; 9688; 2-2021; 1-132045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-021-88874-5info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-021-88874-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:24Zoai:ri.conicet.gov.ar:11336/168111instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:24.862CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds
title ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds
spellingShingle ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds
Tognacca, Rocío Soledad
germination
seeds
ATHB2
Light
title_short ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds
title_full ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds
title_fullStr ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds
title_full_unstemmed ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds
title_sort ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds
dc.creator.none.fl_str_mv Tognacca, Rocío Soledad
Monica, Carabelli
Giorgio, Morelli
Ida, Ruberti
Botto, Javier Francisco
author Tognacca, Rocío Soledad
author_facet Tognacca, Rocío Soledad
Monica, Carabelli
Giorgio, Morelli
Ida, Ruberti
Botto, Javier Francisco
author_role author
author2 Monica, Carabelli
Giorgio, Morelli
Ida, Ruberti
Botto, Javier Francisco
author2_role author
author
author
author
dc.subject.none.fl_str_mv germination
seeds
ATHB2
Light
topic germination
seeds
ATHB2
Light
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The germination timing of seeds is of the utmost adaptive importance for plant populations. Light is one of the best characterized factors promoting seed germination in several species. The germination is also fnely regulated by changes in hormones levels, mainly those of gibberellin (GA) and abscisic acid (ABA). Here, we performed physiological, pharmacological, and molecular analyses to uncover the role of ATHB2, an HD-ZIP II transcription factor, in germination of Arabidopsis seeds. Our study demonstrated that ATHB2 is a negative regulator and sustains the expression of transcription factors to block germination promoted by light. Besides, we found that ATHB2 increases ABA sensitivity. Moreover, ABA and auxin content in athb2-2 mutant is higher than wild-type in dry seeds, but the diferences disappeared during the imbibition in darkness and the frst hours of exposition to light, respectively. Some ABA and light transcription factors are up-regulated by ATHB2, such as ABI5, ABI3, XERICO, SOMNUS and PIL5/PIF1. In opposition, PIN7, an auxin transport, is down-regulated. The role of ATHB2 as a repressor of germination induced by light afecting the gemination timing, could have diferential efects on the establishment of seedlings altering the competitiveness between crops and weeds in the feld.
Fil: Tognacca, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Monica, Carabelli. Consiglio Nazionale delle Ricerche; Italia
Fil: Giorgio, Morelli. Research Centre for Genomics and Bioinformatics, Council for Agricultural Research and Economics; Italia
Fil: Ida, Ruberti. Consiglio Nazionale delle Ricerche; Italia
Fil: Botto, Javier Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
description The germination timing of seeds is of the utmost adaptive importance for plant populations. Light is one of the best characterized factors promoting seed germination in several species. The germination is also fnely regulated by changes in hormones levels, mainly those of gibberellin (GA) and abscisic acid (ABA). Here, we performed physiological, pharmacological, and molecular analyses to uncover the role of ATHB2, an HD-ZIP II transcription factor, in germination of Arabidopsis seeds. Our study demonstrated that ATHB2 is a negative regulator and sustains the expression of transcription factors to block germination promoted by light. Besides, we found that ATHB2 increases ABA sensitivity. Moreover, ABA and auxin content in athb2-2 mutant is higher than wild-type in dry seeds, but the diferences disappeared during the imbibition in darkness and the frst hours of exposition to light, respectively. Some ABA and light transcription factors are up-regulated by ATHB2, such as ABI5, ABI3, XERICO, SOMNUS and PIL5/PIF1. In opposition, PIN7, an auxin transport, is down-regulated. The role of ATHB2 as a repressor of germination induced by light afecting the gemination timing, could have diferential efects on the establishment of seedlings altering the competitiveness between crops and weeds in the feld.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/168111
Tognacca, Rocío Soledad; Monica, Carabelli; Giorgio, Morelli; Ida, Ruberti; Botto, Javier Francisco; ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds; Springer Nature; Scientific Reports; 11; 9688; 2-2021; 1-13
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/168111
identifier_str_mv Tognacca, Rocío Soledad; Monica, Carabelli; Giorgio, Morelli; Ida, Ruberti; Botto, Javier Francisco; ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds; Springer Nature; Scientific Reports; 11; 9688; 2-2021; 1-13
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-021-88874-5
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-021-88874-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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