RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability

Autores
Filomatori, Claudia Veronica; Bardossy, Eugenia Soledad; Merwaiss, Fernando; Suzuki, Yasutsugu; Henrion, Annabelle; Saleh, María Carla; Alvarez, Diego Ezequiel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The potential of RNA viruses to adapt to new environments relies on their ability to introduce changes in their genomes, which has resulted in the recent expansion of re-emergent viruses. Chikungunya virus is an important human pathogen transmitted by mosquitoes that, after 60 years of exclusive circulation in Asia and Africa, has rapidly spread in Europe and the Americas. Here, we examined the evolution of CHIKV in different hosts and uncovered host-specific requirements of the CHIKV 3'UTR. Sequence repeats are conserved at the CHIKV 3'UTR but vary in copy number among viral lineages. We found that these blocks of repeated sequences favor RNA recombination processes through copy-choice mechanism that acts concertedly with viral selection, determining the emergence of new viral variants. Functional analyses using a panel of mutant viruses indicated that opposite selective pressures in mosquito and mammalian cells impose a fitness cost during transmission that is alleviated by recombination guided by sequence repeats. Indeed, drastic changes in the frequency of viral variants with different numbers of repeats were detected during host switch. We propose that RNA recombination accelerates CHIKV adaptability, allowing the virus to overcome genetic bottlenecks within the mosquito host. These studies highlight the role of 3'UTR plasticity on CHIKV evolution, providing a new paradigm to explain the significance of sequence repetitions.
Fil: Filomatori, Claudia Veronica. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Bardossy, Eugenia Soledad. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Merwaiss, Fernando. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Suzuki, Yasutsugu. Centre National de la Recherche Scientifique; Francia. Instituto Pasteur; Francia
Fil: Henrion, Annabelle. Centre National de la Recherche Scientifique; Francia. Instituto Pasteur; Francia
Fil: Saleh, María Carla. Centre National de la Recherche Scientifique; Francia. Instituto Pasteur; Francia
Fil: Alvarez, Diego Ezequiel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Materia
ALPHAVIRUS
RNA RECOMBINATION
TEMPLATE SWITCHING
VIRUS EVOLUTION
CHIKUNGUNYA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184902
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184902
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptabilityFilomatori, Claudia VeronicaBardossy, Eugenia SoledadMerwaiss, FernandoSuzuki, YasutsuguHenrion, AnnabelleSaleh, María CarlaAlvarez, Diego EzequielALPHAVIRUSRNA RECOMBINATIONTEMPLATE SWITCHINGVIRUS EVOLUTIONCHIKUNGUNYAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The potential of RNA viruses to adapt to new environments relies on their ability to introduce changes in their genomes, which has resulted in the recent expansion of re-emergent viruses. Chikungunya virus is an important human pathogen transmitted by mosquitoes that, after 60 years of exclusive circulation in Asia and Africa, has rapidly spread in Europe and the Americas. Here, we examined the evolution of CHIKV in different hosts and uncovered host-specific requirements of the CHIKV 3'UTR. Sequence repeats are conserved at the CHIKV 3'UTR but vary in copy number among viral lineages. We found that these blocks of repeated sequences favor RNA recombination processes through copy-choice mechanism that acts concertedly with viral selection, determining the emergence of new viral variants. Functional analyses using a panel of mutant viruses indicated that opposite selective pressures in mosquito and mammalian cells impose a fitness cost during transmission that is alleviated by recombination guided by sequence repeats. Indeed, drastic changes in the frequency of viral variants with different numbers of repeats were detected during host switch. We propose that RNA recombination accelerates CHIKV adaptability, allowing the virus to overcome genetic bottlenecks within the mosquito host. These studies highlight the role of 3'UTR plasticity on CHIKV evolution, providing a new paradigm to explain the significance of sequence repetitions.Fil: Filomatori, Claudia Veronica. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Bardossy, Eugenia Soledad. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Merwaiss, Fernando. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Suzuki, Yasutsugu. Centre National de la Recherche Scientifique; Francia. Instituto Pasteur; FranciaFil: Henrion, Annabelle. Centre National de la Recherche Scientifique; Francia. Instituto Pasteur; FranciaFil: Saleh, María Carla. Centre National de la Recherche Scientifique; Francia. Instituto Pasteur; FranciaFil: Alvarez, Diego Ezequiel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaPublic Library of Science2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184902Filomatori, Claudia Veronica; Bardossy, Eugenia Soledad; Merwaiss, Fernando; Suzuki, Yasutsugu; Henrion, Annabelle; et al.; RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability; Public Library of Science; Plos Pathogens; 15; 4; 4-2019; 1-271553-7374CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1007706info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.ppat.1007706info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:04Zoai:ri.conicet.gov.ar:11336/184902instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:04.711CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability
title RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability
spellingShingle RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability
Filomatori, Claudia Veronica
ALPHAVIRUS
RNA RECOMBINATION
TEMPLATE SWITCHING
VIRUS EVOLUTION
CHIKUNGUNYA
title_short RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability
title_full RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability
title_fullStr RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability
title_full_unstemmed RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability
title_sort RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability
dc.creator.none.fl_str_mv Filomatori, Claudia Veronica
Bardossy, Eugenia Soledad
Merwaiss, Fernando
Suzuki, Yasutsugu
Henrion, Annabelle
Saleh, María Carla
Alvarez, Diego Ezequiel
author Filomatori, Claudia Veronica
author_facet Filomatori, Claudia Veronica
Bardossy, Eugenia Soledad
Merwaiss, Fernando
Suzuki, Yasutsugu
Henrion, Annabelle
Saleh, María Carla
Alvarez, Diego Ezequiel
author_role author
author2 Bardossy, Eugenia Soledad
Merwaiss, Fernando
Suzuki, Yasutsugu
Henrion, Annabelle
Saleh, María Carla
Alvarez, Diego Ezequiel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ALPHAVIRUS
RNA RECOMBINATION
TEMPLATE SWITCHING
VIRUS EVOLUTION
CHIKUNGUNYA
topic ALPHAVIRUS
RNA RECOMBINATION
TEMPLATE SWITCHING
VIRUS EVOLUTION
CHIKUNGUNYA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The potential of RNA viruses to adapt to new environments relies on their ability to introduce changes in their genomes, which has resulted in the recent expansion of re-emergent viruses. Chikungunya virus is an important human pathogen transmitted by mosquitoes that, after 60 years of exclusive circulation in Asia and Africa, has rapidly spread in Europe and the Americas. Here, we examined the evolution of CHIKV in different hosts and uncovered host-specific requirements of the CHIKV 3'UTR. Sequence repeats are conserved at the CHIKV 3'UTR but vary in copy number among viral lineages. We found that these blocks of repeated sequences favor RNA recombination processes through copy-choice mechanism that acts concertedly with viral selection, determining the emergence of new viral variants. Functional analyses using a panel of mutant viruses indicated that opposite selective pressures in mosquito and mammalian cells impose a fitness cost during transmission that is alleviated by recombination guided by sequence repeats. Indeed, drastic changes in the frequency of viral variants with different numbers of repeats were detected during host switch. We propose that RNA recombination accelerates CHIKV adaptability, allowing the virus to overcome genetic bottlenecks within the mosquito host. These studies highlight the role of 3'UTR plasticity on CHIKV evolution, providing a new paradigm to explain the significance of sequence repetitions.
Fil: Filomatori, Claudia Veronica. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Bardossy, Eugenia Soledad. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Merwaiss, Fernando. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Suzuki, Yasutsugu. Centre National de la Recherche Scientifique; Francia. Instituto Pasteur; Francia
Fil: Henrion, Annabelle. Centre National de la Recherche Scientifique; Francia. Instituto Pasteur; Francia
Fil: Saleh, María Carla. Centre National de la Recherche Scientifique; Francia. Instituto Pasteur; Francia
Fil: Alvarez, Diego Ezequiel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
description The potential of RNA viruses to adapt to new environments relies on their ability to introduce changes in their genomes, which has resulted in the recent expansion of re-emergent viruses. Chikungunya virus is an important human pathogen transmitted by mosquitoes that, after 60 years of exclusive circulation in Asia and Africa, has rapidly spread in Europe and the Americas. Here, we examined the evolution of CHIKV in different hosts and uncovered host-specific requirements of the CHIKV 3'UTR. Sequence repeats are conserved at the CHIKV 3'UTR but vary in copy number among viral lineages. We found that these blocks of repeated sequences favor RNA recombination processes through copy-choice mechanism that acts concertedly with viral selection, determining the emergence of new viral variants. Functional analyses using a panel of mutant viruses indicated that opposite selective pressures in mosquito and mammalian cells impose a fitness cost during transmission that is alleviated by recombination guided by sequence repeats. Indeed, drastic changes in the frequency of viral variants with different numbers of repeats were detected during host switch. We propose that RNA recombination accelerates CHIKV adaptability, allowing the virus to overcome genetic bottlenecks within the mosquito host. These studies highlight the role of 3'UTR plasticity on CHIKV evolution, providing a new paradigm to explain the significance of sequence repetitions.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184902
Filomatori, Claudia Veronica; Bardossy, Eugenia Soledad; Merwaiss, Fernando; Suzuki, Yasutsugu; Henrion, Annabelle; et al.; RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability; Public Library of Science; Plos Pathogens; 15; 4; 4-2019; 1-27
1553-7374
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184902
identifier_str_mv Filomatori, Claudia Veronica; Bardossy, Eugenia Soledad; Merwaiss, Fernando; Suzuki, Yasutsugu; Henrion, Annabelle; et al.; RNA recombination at Chikungunya virus 3'UTR as an evolutionary mechanism that provides adaptability; Public Library of Science; Plos Pathogens; 15; 4; 4-2019; 1-27
1553-7374
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1007706
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.ppat.1007706
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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