Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles

Autores
Spagnoletti, Federico Nicolás; Kronberg, Maria Florencia; Spedalieri, Ana Cecilia; Munarriz, Eliana Rosa; Giacometti, Romina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of environmentally friendly new procedures for the synthesis of metallic nanoparticles is one of the main goals of nanotechnology. Proteins and enzymes from plants, filamentous fungi, yeast, and bacteria to produce nanoparticles are both valuable and viable alternatives to conventional synthesis of nanomaterials due to their high efficiency and the low cost to scale up and generate large quantities. The aim of this work is to compare biogenic silver nanoparticles (AgNPs) obtained from cell-free filtrates from the fungus Macrophomina phaseolina to conventional chemical AgNPs, in biocidal activity and toxicity. Our results show that bio-AgNPs displayed similar bactericidal activity than chemical AgNPs, but less toxicity in the model organism Caenorhabditis elegans. We employed biochemical and proteomic techniques to profile the unique surface chemistry of the capping in the bio-AgNPs and therefore to identify the proteins involved in their synthesis and stability. These results not only suggest that the proteins involved in the synthesis of the nanoparticles and corona formation in the bio-AgNPs are responsible for keeping the silver core preserved making them more stable in time, but also masking and protecting eukaryotic cells from metal toxicity.
Fil: Spagnoletti, Federico Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Kronberg, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Spedalieri, Ana Cecilia. Humboldt-Universität zu Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Munarriz, Eliana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Giacometti, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Materia
CAPPING
GREEN NANOTECHNOLOGY
PROTEOMICS
SILVER NANOPARTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/177519
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/177519
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticlesSpagnoletti, Federico NicolásKronberg, Maria FlorenciaSpedalieri, Ana CeciliaMunarriz, Eliana RosaGiacometti, RominaCAPPINGGREEN NANOTECHNOLOGYPROTEOMICSSILVER NANOPARTICLEShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2The development of environmentally friendly new procedures for the synthesis of metallic nanoparticles is one of the main goals of nanotechnology. Proteins and enzymes from plants, filamentous fungi, yeast, and bacteria to produce nanoparticles are both valuable and viable alternatives to conventional synthesis of nanomaterials due to their high efficiency and the low cost to scale up and generate large quantities. The aim of this work is to compare biogenic silver nanoparticles (AgNPs) obtained from cell-free filtrates from the fungus Macrophomina phaseolina to conventional chemical AgNPs, in biocidal activity and toxicity. Our results show that bio-AgNPs displayed similar bactericidal activity than chemical AgNPs, but less toxicity in the model organism Caenorhabditis elegans. We employed biochemical and proteomic techniques to profile the unique surface chemistry of the capping in the bio-AgNPs and therefore to identify the proteins involved in their synthesis and stability. These results not only suggest that the proteins involved in the synthesis of the nanoparticles and corona formation in the bio-AgNPs are responsible for keeping the silver core preserved making them more stable in time, but also masking and protecting eukaryotic cells from metal toxicity.Fil: Spagnoletti, Federico Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Kronberg, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Spedalieri, Ana Cecilia. Humboldt-Universität zu Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Munarriz, Eliana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Giacometti, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/177519Spagnoletti, Federico Nicolás; Kronberg, Maria Florencia; Spedalieri, Ana Cecilia; Munarriz, Eliana Rosa; Giacometti, Romina; Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles; Academic Press Ltd - Elsevier Science Ltd; Journal of Environmental Management; 297; 113434; 11-2021; 1-120301-4797CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jenvman.2021.113434info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0301479721014961info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:19Zoai:ri.conicet.gov.ar:11336/177519instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:20.053CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles
title Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles
spellingShingle Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles
Spagnoletti, Federico Nicolás
CAPPING
GREEN NANOTECHNOLOGY
PROTEOMICS
SILVER NANOPARTICLES
title_short Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles
title_full Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles
title_fullStr Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles
title_full_unstemmed Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles
title_sort Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles
dc.creator.none.fl_str_mv Spagnoletti, Federico Nicolás
Kronberg, Maria Florencia
Spedalieri, Ana Cecilia
Munarriz, Eliana Rosa
Giacometti, Romina
author Spagnoletti, Federico Nicolás
author_facet Spagnoletti, Federico Nicolás
Kronberg, Maria Florencia
Spedalieri, Ana Cecilia
Munarriz, Eliana Rosa
Giacometti, Romina
author_role author
author2 Kronberg, Maria Florencia
Spedalieri, Ana Cecilia
Munarriz, Eliana Rosa
Giacometti, Romina
author2_role author
author
author
author
dc.subject.none.fl_str_mv CAPPING
GREEN NANOTECHNOLOGY
PROTEOMICS
SILVER NANOPARTICLES
topic CAPPING
GREEN NANOTECHNOLOGY
PROTEOMICS
SILVER NANOPARTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The development of environmentally friendly new procedures for the synthesis of metallic nanoparticles is one of the main goals of nanotechnology. Proteins and enzymes from plants, filamentous fungi, yeast, and bacteria to produce nanoparticles are both valuable and viable alternatives to conventional synthesis of nanomaterials due to their high efficiency and the low cost to scale up and generate large quantities. The aim of this work is to compare biogenic silver nanoparticles (AgNPs) obtained from cell-free filtrates from the fungus Macrophomina phaseolina to conventional chemical AgNPs, in biocidal activity and toxicity. Our results show that bio-AgNPs displayed similar bactericidal activity than chemical AgNPs, but less toxicity in the model organism Caenorhabditis elegans. We employed biochemical and proteomic techniques to profile the unique surface chemistry of the capping in the bio-AgNPs and therefore to identify the proteins involved in their synthesis and stability. These results not only suggest that the proteins involved in the synthesis of the nanoparticles and corona formation in the bio-AgNPs are responsible for keeping the silver core preserved making them more stable in time, but also masking and protecting eukaryotic cells from metal toxicity.
Fil: Spagnoletti, Federico Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Kronberg, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Spedalieri, Ana Cecilia. Humboldt-Universität zu Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Munarriz, Eliana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Giacometti, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
description The development of environmentally friendly new procedures for the synthesis of metallic nanoparticles is one of the main goals of nanotechnology. Proteins and enzymes from plants, filamentous fungi, yeast, and bacteria to produce nanoparticles are both valuable and viable alternatives to conventional synthesis of nanomaterials due to their high efficiency and the low cost to scale up and generate large quantities. The aim of this work is to compare biogenic silver nanoparticles (AgNPs) obtained from cell-free filtrates from the fungus Macrophomina phaseolina to conventional chemical AgNPs, in biocidal activity and toxicity. Our results show that bio-AgNPs displayed similar bactericidal activity than chemical AgNPs, but less toxicity in the model organism Caenorhabditis elegans. We employed biochemical and proteomic techniques to profile the unique surface chemistry of the capping in the bio-AgNPs and therefore to identify the proteins involved in their synthesis and stability. These results not only suggest that the proteins involved in the synthesis of the nanoparticles and corona formation in the bio-AgNPs are responsible for keeping the silver core preserved making them more stable in time, but also masking and protecting eukaryotic cells from metal toxicity.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/177519
Spagnoletti, Federico Nicolás; Kronberg, Maria Florencia; Spedalieri, Ana Cecilia; Munarriz, Eliana Rosa; Giacometti, Romina; Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles; Academic Press Ltd - Elsevier Science Ltd; Journal of Environmental Management; 297; 113434; 11-2021; 1-12
0301-4797
CONICET Digital
CONICET
url http://hdl.handle.net/11336/177519
identifier_str_mv Spagnoletti, Federico Nicolás; Kronberg, Maria Florencia; Spedalieri, Ana Cecilia; Munarriz, Eliana Rosa; Giacometti, Romina; Protein corona on biogenic silver nanoparticles provides higher stability and protects cells from toxicity in comparison to chemical nanoparticles; Academic Press Ltd - Elsevier Science Ltd; Journal of Environmental Management; 297; 113434; 11-2021; 1-12
0301-4797
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jenvman.2021.113434
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0301479721014961
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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