Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor

Autores
Verbitsky, Miguel; Plazas, Paola Viviana; Elgoyhen, Ana Belen
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We describe the functional properties of a nicotinic alpha9/serotonin subtype 3A (5HT3A) chimeric receptor expressed in Xenopus laevis oocytes. The chimera preserved ligand-binding properties of alpha9 and channel properties of 5HT3A. Thus, it responded to acetylcholine in a concentration-dependent manner with an EC50 of 70 microM but not to serotonin. It was blocked by methyllycaconitine, strychnine, atropine and nicotine, with the same rank order of potency as alpha9 receptors. The current-voltage relationship of currents through the alpha9/5HT3A chimera was similar to that of the 5HT3A receptors. These results are an evidence of functional coupling between the ligand-binding and the channel domains of the chimeric receptor.
Fil: Verbitsky, Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Plazas, Paola Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
Hair Cells
Ion Channels
Nicotinic Receptors
Serotonin Receptors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79737
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79737
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptorVerbitsky, MiguelPlazas, Paola VivianaElgoyhen, Ana BelenHair CellsIon ChannelsNicotinic ReceptorsSerotonin Receptorshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We describe the functional properties of a nicotinic alpha9/serotonin subtype 3A (5HT3A) chimeric receptor expressed in Xenopus laevis oocytes. The chimera preserved ligand-binding properties of alpha9 and channel properties of 5HT3A. Thus, it responded to acetylcholine in a concentration-dependent manner with an EC50 of 70 microM but not to serotonin. It was blocked by methyllycaconitine, strychnine, atropine and nicotine, with the same rank order of potency as alpha9 receptors. The current-voltage relationship of currents through the alpha9/5HT3A chimera was similar to that of the 5HT3A receptors. These results are an evidence of functional coupling between the ligand-binding and the channel domains of the chimeric receptor.Fil: Verbitsky, Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Plazas, Paola Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaLippincott Williams2003-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79737Verbitsky, Miguel; Plazas, Paola Viviana; Elgoyhen, Ana Belen; Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor; Lippincott Williams; Neuroreport; 14; 15; 10-2003; 1931-19340959-4965CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/14561923info:eu-repo/semantics/altIdentifier/doi/10.1097/01.wnr.0000093755.20088.f5info:eu-repo/semantics/altIdentifier/url/https://journals.lww.com/neuroreport/Abstract/2003/10270/Functional_expression_and_properties_of_a.10.aspxinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:50Zoai:ri.conicet.gov.ar:11336/79737instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:50.581CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor
title Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor
spellingShingle Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor
Verbitsky, Miguel
Hair Cells
Ion Channels
Nicotinic Receptors
Serotonin Receptors
title_short Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor
title_full Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor
title_fullStr Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor
title_full_unstemmed Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor
title_sort Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor
dc.creator.none.fl_str_mv Verbitsky, Miguel
Plazas, Paola Viviana
Elgoyhen, Ana Belen
author Verbitsky, Miguel
author_facet Verbitsky, Miguel
Plazas, Paola Viviana
Elgoyhen, Ana Belen
author_role author
author2 Plazas, Paola Viviana
Elgoyhen, Ana Belen
author2_role author
author
dc.subject.none.fl_str_mv Hair Cells
Ion Channels
Nicotinic Receptors
Serotonin Receptors
topic Hair Cells
Ion Channels
Nicotinic Receptors
Serotonin Receptors
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We describe the functional properties of a nicotinic alpha9/serotonin subtype 3A (5HT3A) chimeric receptor expressed in Xenopus laevis oocytes. The chimera preserved ligand-binding properties of alpha9 and channel properties of 5HT3A. Thus, it responded to acetylcholine in a concentration-dependent manner with an EC50 of 70 microM but not to serotonin. It was blocked by methyllycaconitine, strychnine, atropine and nicotine, with the same rank order of potency as alpha9 receptors. The current-voltage relationship of currents through the alpha9/5HT3A chimera was similar to that of the 5HT3A receptors. These results are an evidence of functional coupling between the ligand-binding and the channel domains of the chimeric receptor.
Fil: Verbitsky, Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Plazas, Paola Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description We describe the functional properties of a nicotinic alpha9/serotonin subtype 3A (5HT3A) chimeric receptor expressed in Xenopus laevis oocytes. The chimera preserved ligand-binding properties of alpha9 and channel properties of 5HT3A. Thus, it responded to acetylcholine in a concentration-dependent manner with an EC50 of 70 microM but not to serotonin. It was blocked by methyllycaconitine, strychnine, atropine and nicotine, with the same rank order of potency as alpha9 receptors. The current-voltage relationship of currents through the alpha9/5HT3A chimera was similar to that of the 5HT3A receptors. These results are an evidence of functional coupling between the ligand-binding and the channel domains of the chimeric receptor.
publishDate 2003
dc.date.none.fl_str_mv 2003-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79737
Verbitsky, Miguel; Plazas, Paola Viviana; Elgoyhen, Ana Belen; Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor; Lippincott Williams; Neuroreport; 14; 15; 10-2003; 1931-1934
0959-4965
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79737
identifier_str_mv Verbitsky, Miguel; Plazas, Paola Viviana; Elgoyhen, Ana Belen; Functional expression and properties of a nicotinic alpha9/5-HT3A chimeric receptor; Lippincott Williams; Neuroreport; 14; 15; 10-2003; 1931-1934
0959-4965
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/14561923
info:eu-repo/semantics/altIdentifier/doi/10.1097/01.wnr.0000093755.20088.f5
info:eu-repo/semantics/altIdentifier/url/https://journals.lww.com/neuroreport/Abstract/2003/10270/Functional_expression_and_properties_of_a.10.aspx
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268626783043584
score 13.13397

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