Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma

Autores
Jimenez, Oscar Eduardo; Colli, Sandra Lorena; Garcia Lombardi, M.; Preciado, Maria Victoria; de Matteo, Elena Noemí; Chabay, Paola Andrea
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Classic Hodgkin lymphoma (cHL) is a lymphoid neoplasm in which the immune microenvironment contributes to the lymphomagenesis process. Epstein Barr virus (EBV) presence also influences cHL microenvironment composition and contributes to pathogenesis. Our aim was to evaluate the PD1 / PDL1 pathway and EBV influence on this pathway in pediatric cHL. Methods: 80 pediatric patients were analyzed. EBV presence was assessed by in situ hybridization (HIS), the expression of PDL1 in the microenvironment (PDL1mic) and PDL1 in the HRS tumor cells (PDL1HRS) and PD1 in the microenvironment (PD1mic) by immunohistochemistry (IHC) expressing the results as +cells/ mm2. PDL1 genetic alterations were analyzed in a subgroup of 37 pediatric patients by FISH, following the criteria of: genetic gain (PDL1 / CEP9 <3: 1), amplification (PDL1 / CEP9 ≥3: 1) or diplody (PDL1 / CEP9=1:1). The survival was evaluated in relation to the expression of PDL1 mic, PDL1 HRS and PD1 mic. Results: No significant differences were observed in the PD1mic count or in the PDL1 HRS count between the EBV + and EBV- cases (P> 0.05; Mann Whitney test). Unexpectedly, only 38% of pediatric cHL showed PDL1 genetic alterations by FISH (8% amplification, 16% gain and 13% gain + amplification) and no differences were observed in EBV + vs EBV- cases (p> 0.05, exact test of Fisher). In the cHL EBV + cases, a significant increase in PDL1mic + cells was detected in the microenvironment (p> 0.05, Mann Whitney test). Neither PD1mic nor PDL1 expression in HRS cells or in the microenvironment was associated with survival in pediatric patients (p> 0.05, log-rank test) .Conclusions: Although our group previously described an environment of high cytotoxicity in pediatric EBV + cHL, it could be counteracted by a PDL1 + cell niche in the microenvironment, leading to unsuccessful elimination of EBV+ HRS tumor cells.
Fil: Jimenez, Oscar Eduardo. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Colli, Sandra Lorena. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina
Fil: Garcia Lombardi, M.. Hospital Zonal General de Agudos doctor Ricardo Gutierrez ; Gobierno de la Provincia de Buenos Aires;
Fil: Preciado, Maria Victoria. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina Fisiología
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina Fisiología
Materia
Linfomas de Hodgkin
Epstein Barr
Microambiente
PDL1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212208
Acceder
id CONICETDig_8205c9874df9742b598d567585e1086f
oai_identifier_str oai:ri.conicet.gov.ar:11336/212208
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin LymphomaJimenez, Oscar EduardoColli, Sandra LorenaGarcia Lombardi, M.Preciado, Maria Victoriade Matteo, Elena NoemíChabay, Paola AndreaLinfomas de HodgkinEpstein BarrMicroambientePDL1https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Classic Hodgkin lymphoma (cHL) is a lymphoid neoplasm in which the immune microenvironment contributes to the lymphomagenesis process. Epstein Barr virus (EBV) presence also influences cHL microenvironment composition and contributes to pathogenesis. Our aim was to evaluate the PD1 / PDL1 pathway and EBV influence on this pathway in pediatric cHL. Methods: 80 pediatric patients were analyzed. EBV presence was assessed by in situ hybridization (HIS), the expression of PDL1 in the microenvironment (PDL1mic) and PDL1 in the HRS tumor cells (PDL1HRS) and PD1 in the microenvironment (PD1mic) by immunohistochemistry (IHC) expressing the results as +cells/ mm2. PDL1 genetic alterations were analyzed in a subgroup of 37 pediatric patients by FISH, following the criteria of: genetic gain (PDL1 / CEP9 <3: 1), amplification (PDL1 / CEP9 ≥3: 1) or diplody (PDL1 / CEP9=1:1). The survival was evaluated in relation to the expression of PDL1 mic, PDL1 HRS and PD1 mic. Results: No significant differences were observed in the PD1mic count or in the PDL1 HRS count between the EBV + and EBV- cases (P> 0.05; Mann Whitney test). Unexpectedly, only 38% of pediatric cHL showed PDL1 genetic alterations by FISH (8% amplification, 16% gain and 13% gain + amplification) and no differences were observed in EBV + vs EBV- cases (p> 0.05, exact test of Fisher). In the cHL EBV + cases, a significant increase in PDL1mic + cells was detected in the microenvironment (p> 0.05, Mann Whitney test). Neither PD1mic nor PDL1 expression in HRS cells or in the microenvironment was associated with survival in pediatric patients (p> 0.05, log-rank test) .Conclusions: Although our group previously described an environment of high cytotoxicity in pediatric EBV + cHL, it could be counteracted by a PDL1 + cell niche in the microenvironment, leading to unsuccessful elimination of EBV+ HRS tumor cells.Fil: Jimenez, Oscar Eduardo. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Colli, Sandra Lorena. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; ArgentinaFil: Garcia Lombardi, M.. Hospital Zonal General de Agudos doctor Ricardo Gutierrez ; Gobierno de la Provincia de Buenos Aires;Fil: Preciado, Maria Victoria. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina FisiologíaArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212208Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina Fisiología; Argentina; 2020; 1-10025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol80-20/s5/Mv80s5.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:00:26Zoai:ri.conicet.gov.ar:11336/212208instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:00:27.141CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma
title Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma
spellingShingle Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma
Jimenez, Oscar Eduardo
Linfomas de Hodgkin
Epstein Barr
Microambiente
PDL1
title_short Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma
title_full Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma
title_fullStr Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma
title_full_unstemmed Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma
title_sort Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma
dc.creator.none.fl_str_mv Jimenez, Oscar Eduardo
Colli, Sandra Lorena
Garcia Lombardi, M.
Preciado, Maria Victoria
de Matteo, Elena Noemí
Chabay, Paola Andrea
author Jimenez, Oscar Eduardo
author_facet Jimenez, Oscar Eduardo
Colli, Sandra Lorena
Garcia Lombardi, M.
Preciado, Maria Victoria
de Matteo, Elena Noemí
Chabay, Paola Andrea
author_role author
author2 Colli, Sandra Lorena
Garcia Lombardi, M.
Preciado, Maria Victoria
de Matteo, Elena Noemí
Chabay, Paola Andrea
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Linfomas de Hodgkin
Epstein Barr
Microambiente
PDL1
topic Linfomas de Hodgkin
Epstein Barr
Microambiente
PDL1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Classic Hodgkin lymphoma (cHL) is a lymphoid neoplasm in which the immune microenvironment contributes to the lymphomagenesis process. Epstein Barr virus (EBV) presence also influences cHL microenvironment composition and contributes to pathogenesis. Our aim was to evaluate the PD1 / PDL1 pathway and EBV influence on this pathway in pediatric cHL. Methods: 80 pediatric patients were analyzed. EBV presence was assessed by in situ hybridization (HIS), the expression of PDL1 in the microenvironment (PDL1mic) and PDL1 in the HRS tumor cells (PDL1HRS) and PD1 in the microenvironment (PD1mic) by immunohistochemistry (IHC) expressing the results as +cells/ mm2. PDL1 genetic alterations were analyzed in a subgroup of 37 pediatric patients by FISH, following the criteria of: genetic gain (PDL1 / CEP9 <3: 1), amplification (PDL1 / CEP9 ≥3: 1) or diplody (PDL1 / CEP9=1:1). The survival was evaluated in relation to the expression of PDL1 mic, PDL1 HRS and PD1 mic. Results: No significant differences were observed in the PD1mic count or in the PDL1 HRS count between the EBV + and EBV- cases (P> 0.05; Mann Whitney test). Unexpectedly, only 38% of pediatric cHL showed PDL1 genetic alterations by FISH (8% amplification, 16% gain and 13% gain + amplification) and no differences were observed in EBV + vs EBV- cases (p> 0.05, exact test of Fisher). In the cHL EBV + cases, a significant increase in PDL1mic + cells was detected in the microenvironment (p> 0.05, Mann Whitney test). Neither PD1mic nor PDL1 expression in HRS cells or in the microenvironment was associated with survival in pediatric patients (p> 0.05, log-rank test) .Conclusions: Although our group previously described an environment of high cytotoxicity in pediatric EBV + cHL, it could be counteracted by a PDL1 + cell niche in the microenvironment, leading to unsuccessful elimination of EBV+ HRS tumor cells.
Fil: Jimenez, Oscar Eduardo. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Colli, Sandra Lorena. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina
Fil: Garcia Lombardi, M.. Hospital Zonal General de Agudos doctor Ricardo Gutierrez ; Gobierno de la Provincia de Buenos Aires;
Fil: Preciado, Maria Victoria. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina Fisiología
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina Fisiología
description Classic Hodgkin lymphoma (cHL) is a lymphoid neoplasm in which the immune microenvironment contributes to the lymphomagenesis process. Epstein Barr virus (EBV) presence also influences cHL microenvironment composition and contributes to pathogenesis. Our aim was to evaluate the PD1 / PDL1 pathway and EBV influence on this pathway in pediatric cHL. Methods: 80 pediatric patients were analyzed. EBV presence was assessed by in situ hybridization (HIS), the expression of PDL1 in the microenvironment (PDL1mic) and PDL1 in the HRS tumor cells (PDL1HRS) and PD1 in the microenvironment (PD1mic) by immunohistochemistry (IHC) expressing the results as +cells/ mm2. PDL1 genetic alterations were analyzed in a subgroup of 37 pediatric patients by FISH, following the criteria of: genetic gain (PDL1 / CEP9 <3: 1), amplification (PDL1 / CEP9 ≥3: 1) or diplody (PDL1 / CEP9=1:1). The survival was evaluated in relation to the expression of PDL1 mic, PDL1 HRS and PD1 mic. Results: No significant differences were observed in the PD1mic count or in the PDL1 HRS count between the EBV + and EBV- cases (P> 0.05; Mann Whitney test). Unexpectedly, only 38% of pediatric cHL showed PDL1 genetic alterations by FISH (8% amplification, 16% gain and 13% gain + amplification) and no differences were observed in EBV + vs EBV- cases (p> 0.05, exact test of Fisher). In the cHL EBV + cases, a significant increase in PDL1mic + cells was detected in the microenvironment (p> 0.05, Mann Whitney test). Neither PD1mic nor PDL1 expression in HRS cells or in the microenvironment was associated with survival in pediatric patients (p> 0.05, log-rank test) .Conclusions: Although our group previously described an environment of high cytotoxicity in pediatric EBV + cHL, it could be counteracted by a PDL1 + cell niche in the microenvironment, leading to unsuccessful elimination of EBV+ HRS tumor cells.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212208
Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina Fisiología; Argentina; 2020; 1-1
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212208
identifier_str_mv Epstein Barr Virus Recruits PDL1 Positive Cells at the Microenvironment in Pediatric Hodgkin Lymphoma; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina Fisiología; Argentina; 2020; 1-1
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol80-20/s5/Mv80s5.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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