Stress-induced decrement in the plasticity of the physical properties of chick brain membranes

Autores
Garcia, Daniel Asmed; Marin, Raul Hector; Perillo, Maria Angelica
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The molecular basis underlying the stress-induced increment in the density of central benzodiazepine receptor from chick forebrain, observed previously at 4°C, was studied from a biophysical perspective. The thermal dependence of [3H]flunitrazepam binding to the central benzodiazepine receptor and the supramolecular organization were studied in forebrain membranes from chicks submitted to partial water immersion. The equilibrium dissociation constants increased with temperature in membrane from both control and stressed chicks. The heat capacity values in control samples (ΔCp, CON) were significantly less negative than ΔCp STR. Changes in ΔH and ΔS between 4-37°C were greater in stressed chicks compared to control; however, the binding was exothermic and driven by enthalpy in both conditions. At 4°C, the receptor density (Bmax) was higher in stressed chicks compared to control. Such a difference was lost irreversibly upon temperature elevation, possibly due to the hysteresis between the heating and cooling behaviour of Bmax, CON and the constancy in Bmax, STR. The fluorescence anisotropy of diphenylhexatriene was higher in control samples with respect to stressed chicks below 10°C. A temperature-induced increment in protein intrinsic-fluorescence was observed only in control, and was quenched by acrylamide more easily at 4°C than at 25°C. A higher microviscosity at 4°C in control favoured more external localizations of integral proteins; at higher temperatures, tryptophan residues moved to hydrophobic membrane-regions. Changes in the membrane-organization towards more fluid states favoured the accessibility of benzodiazepine to the central benzodiazepine receptor, expressed by the higher values of Bmax found in stressed samples at low temperatures with respect to control samples.
Fil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Marin, Raul Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Materia
Benzodiazepine Binding
Dph Fluorescence
Dynamic Quenching
Gabaa Receptor
Membrane Fluidity
Stress
Thermodynamics
Tryptophan Fluorescence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65677
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/65677
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Stress-induced decrement in the plasticity of the physical properties of chick brain membranesGarcia, Daniel AsmedMarin, Raul HectorPerillo, Maria AngelicaBenzodiazepine BindingDph FluorescenceDynamic QuenchingGabaa ReceptorMembrane FluidityStressThermodynamicsTryptophan Fluorescencehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The molecular basis underlying the stress-induced increment in the density of central benzodiazepine receptor from chick forebrain, observed previously at 4°C, was studied from a biophysical perspective. The thermal dependence of [3H]flunitrazepam binding to the central benzodiazepine receptor and the supramolecular organization were studied in forebrain membranes from chicks submitted to partial water immersion. The equilibrium dissociation constants increased with temperature in membrane from both control and stressed chicks. The heat capacity values in control samples (ΔCp, CON) were significantly less negative than ΔCp STR. Changes in ΔH and ΔS between 4-37°C were greater in stressed chicks compared to control; however, the binding was exothermic and driven by enthalpy in both conditions. At 4°C, the receptor density (Bmax) was higher in stressed chicks compared to control. Such a difference was lost irreversibly upon temperature elevation, possibly due to the hysteresis between the heating and cooling behaviour of Bmax, CON and the constancy in Bmax, STR. The fluorescence anisotropy of diphenylhexatriene was higher in control samples with respect to stressed chicks below 10°C. A temperature-induced increment in protein intrinsic-fluorescence was observed only in control, and was quenched by acrylamide more easily at 4°C than at 25°C. A higher microviscosity at 4°C in control favoured more external localizations of integral proteins; at higher temperatures, tryptophan residues moved to hydrophobic membrane-regions. Changes in the membrane-organization towards more fluid states favoured the accessibility of benzodiazepine to the central benzodiazepine receptor, expressed by the higher values of Bmax found in stressed samples at low temperatures with respect to control samples.Fil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Marin, Raul Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaTaylor & Francis Ltd2002-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65677Garcia, Daniel Asmed; Marin, Raul Hector; Perillo, Maria Angelica; Stress-induced decrement in the plasticity of the physical properties of chick brain membranes; Taylor & Francis Ltd; Molecular Membrane Biology; 19; 3; 7-2002; 221-2300968-7688CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/09687680210156786info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/09687680210156786info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:43Zoai:ri.conicet.gov.ar:11336/65677instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:43.61CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Stress-induced decrement in the plasticity of the physical properties of chick brain membranes
title Stress-induced decrement in the plasticity of the physical properties of chick brain membranes
spellingShingle Stress-induced decrement in the plasticity of the physical properties of chick brain membranes
Garcia, Daniel Asmed
Benzodiazepine Binding
Dph Fluorescence
Dynamic Quenching
Gabaa Receptor
Membrane Fluidity
Stress
Thermodynamics
Tryptophan Fluorescence
title_short Stress-induced decrement in the plasticity of the physical properties of chick brain membranes
title_full Stress-induced decrement in the plasticity of the physical properties of chick brain membranes
title_fullStr Stress-induced decrement in the plasticity of the physical properties of chick brain membranes
title_full_unstemmed Stress-induced decrement in the plasticity of the physical properties of chick brain membranes
title_sort Stress-induced decrement in the plasticity of the physical properties of chick brain membranes
dc.creator.none.fl_str_mv Garcia, Daniel Asmed
Marin, Raul Hector
Perillo, Maria Angelica
author Garcia, Daniel Asmed
author_facet Garcia, Daniel Asmed
Marin, Raul Hector
Perillo, Maria Angelica
author_role author
author2 Marin, Raul Hector
Perillo, Maria Angelica
author2_role author
author
dc.subject.none.fl_str_mv Benzodiazepine Binding
Dph Fluorescence
Dynamic Quenching
Gabaa Receptor
Membrane Fluidity
Stress
Thermodynamics
Tryptophan Fluorescence
topic Benzodiazepine Binding
Dph Fluorescence
Dynamic Quenching
Gabaa Receptor
Membrane Fluidity
Stress
Thermodynamics
Tryptophan Fluorescence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The molecular basis underlying the stress-induced increment in the density of central benzodiazepine receptor from chick forebrain, observed previously at 4°C, was studied from a biophysical perspective. The thermal dependence of [3H]flunitrazepam binding to the central benzodiazepine receptor and the supramolecular organization were studied in forebrain membranes from chicks submitted to partial water immersion. The equilibrium dissociation constants increased with temperature in membrane from both control and stressed chicks. The heat capacity values in control samples (ΔCp, CON) were significantly less negative than ΔCp STR. Changes in ΔH and ΔS between 4-37°C were greater in stressed chicks compared to control; however, the binding was exothermic and driven by enthalpy in both conditions. At 4°C, the receptor density (Bmax) was higher in stressed chicks compared to control. Such a difference was lost irreversibly upon temperature elevation, possibly due to the hysteresis between the heating and cooling behaviour of Bmax, CON and the constancy in Bmax, STR. The fluorescence anisotropy of diphenylhexatriene was higher in control samples with respect to stressed chicks below 10°C. A temperature-induced increment in protein intrinsic-fluorescence was observed only in control, and was quenched by acrylamide more easily at 4°C than at 25°C. A higher microviscosity at 4°C in control favoured more external localizations of integral proteins; at higher temperatures, tryptophan residues moved to hydrophobic membrane-regions. Changes in the membrane-organization towards more fluid states favoured the accessibility of benzodiazepine to the central benzodiazepine receptor, expressed by the higher values of Bmax found in stressed samples at low temperatures with respect to control samples.
Fil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Marin, Raul Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
description The molecular basis underlying the stress-induced increment in the density of central benzodiazepine receptor from chick forebrain, observed previously at 4°C, was studied from a biophysical perspective. The thermal dependence of [3H]flunitrazepam binding to the central benzodiazepine receptor and the supramolecular organization were studied in forebrain membranes from chicks submitted to partial water immersion. The equilibrium dissociation constants increased with temperature in membrane from both control and stressed chicks. The heat capacity values in control samples (ΔCp, CON) were significantly less negative than ΔCp STR. Changes in ΔH and ΔS between 4-37°C were greater in stressed chicks compared to control; however, the binding was exothermic and driven by enthalpy in both conditions. At 4°C, the receptor density (Bmax) was higher in stressed chicks compared to control. Such a difference was lost irreversibly upon temperature elevation, possibly due to the hysteresis between the heating and cooling behaviour of Bmax, CON and the constancy in Bmax, STR. The fluorescence anisotropy of diphenylhexatriene was higher in control samples with respect to stressed chicks below 10°C. A temperature-induced increment in protein intrinsic-fluorescence was observed only in control, and was quenched by acrylamide more easily at 4°C than at 25°C. A higher microviscosity at 4°C in control favoured more external localizations of integral proteins; at higher temperatures, tryptophan residues moved to hydrophobic membrane-regions. Changes in the membrane-organization towards more fluid states favoured the accessibility of benzodiazepine to the central benzodiazepine receptor, expressed by the higher values of Bmax found in stressed samples at low temperatures with respect to control samples.
publishDate 2002
dc.date.none.fl_str_mv 2002-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65677
Garcia, Daniel Asmed; Marin, Raul Hector; Perillo, Maria Angelica; Stress-induced decrement in the plasticity of the physical properties of chick brain membranes; Taylor & Francis Ltd; Molecular Membrane Biology; 19; 3; 7-2002; 221-230
0968-7688
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65677
identifier_str_mv Garcia, Daniel Asmed; Marin, Raul Hector; Perillo, Maria Angelica; Stress-induced decrement in the plasticity of the physical properties of chick brain membranes; Taylor & Francis Ltd; Molecular Membrane Biology; 19; 3; 7-2002; 221-230
0968-7688
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/09687680210156786
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/09687680210156786
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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