Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits

Autores
Medina, María Fernanda; Alberto, Maria Rosa; Sierra, Liliana; Van Nieuwenhove, Carina Paola; Saad, Silvia; Isla, Maria Ines; Jerez, Susana Josefina
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study evaluatedthe plasma fatty acidlevels and thevascular prostaglandin releaseina rabbit model of early hypercholesterolemia with endothelial dysfunction. Rabbits were fed either a control diet (CD) or a diet containing 1 % cholesterol (HD) for 5-6 weeks. The level of fatty acids was measured in plasma. The levels of prostaglandin and nitric oxide (NO) released from aorta were also determined. Vascular morphology of aorta was characterized by intima and media thickness measurement. The rabbits fed with HD had higher levels of arachidonic acid (ARA) and lower levels of oleic acid (OLA). Linoleic acid (LNA) level was unchanged. PGI2 and NO were diminished and PGF2αlevels, the PGI2/TXA2 ratio and the intima/media ratio were increased in rabbits fed with HD. In conclusion, feeding a HD for a short period increased ARA plasma levels and unbalanced release of vasodilator/vasoconstrictor PG redirecting the pathway to vasoconstrictor metabolite release. These lipid metabolism alterations in addition to the reduced NO levels and the moderate changes in the vascular morphology would contribute to the endothelial dysfunction in this animal model. Therefore, the present findings support the importance ofearly correction or prevention of high cholesterol levels to disruptthe endothelial dysfunction processthat leadsto cardiovascular disease.
Fil: Medina, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Alberto, Maria Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto de Química del Noroeste. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Sierra, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Van Nieuwenhove, Carina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Saad, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto de Química del Noroeste. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Jerez, Susana Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Materia
Hypercholesterolemia
Arachidonic Acid
Prostaglandins
Rabbit Aorta
Endothelial Dysfunction
Fatty Acids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19420
Acceder
id CONICETDig_8198b92125c0dc9886229f1423083fcf
oai_identifier_str oai:ri.conicet.gov.ar:11336/19420
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbitsMedina, María FernandaAlberto, Maria RosaSierra, LilianaVan Nieuwenhove, Carina PaolaSaad, SilviaIsla, Maria InesJerez, Susana JosefinaHypercholesterolemiaArachidonic AcidProstaglandinsRabbit AortaEndothelial DysfunctionFatty Acidshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present study evaluatedthe plasma fatty acidlevels and thevascular prostaglandin releaseina rabbit model of early hypercholesterolemia with endothelial dysfunction. Rabbits were fed either a control diet (CD) or a diet containing 1 % cholesterol (HD) for 5-6 weeks. The level of fatty acids was measured in plasma. The levels of prostaglandin and nitric oxide (NO) released from aorta were also determined. Vascular morphology of aorta was characterized by intima and media thickness measurement. The rabbits fed with HD had higher levels of arachidonic acid (ARA) and lower levels of oleic acid (OLA). Linoleic acid (LNA) level was unchanged. PGI2 and NO were diminished and PGF2αlevels, the PGI2/TXA2 ratio and the intima/media ratio were increased in rabbits fed with HD. In conclusion, feeding a HD for a short period increased ARA plasma levels and unbalanced release of vasodilator/vasoconstrictor PG redirecting the pathway to vasoconstrictor metabolite release. These lipid metabolism alterations in addition to the reduced NO levels and the moderate changes in the vascular morphology would contribute to the endothelial dysfunction in this animal model. Therefore, the present findings support the importance ofearly correction or prevention of high cholesterol levels to disruptthe endothelial dysfunction processthat leadsto cardiovascular disease.Fil: Medina, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Alberto, Maria Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto de Química del Noroeste. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Sierra, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaFil: Van Nieuwenhove, Carina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Saad, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaFil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto de Química del Noroeste. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Jerez, Susana Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaSpringer2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://dx.doi.org/10.1007/s11745-014-3915-6https://link.springer.com/article/10.1007/s11745-014-3915-6http://hdl.handle.net/11336/19420Medina, María Fernanda; Alberto, Maria Rosa; Sierra, Liliana; Van Nieuwenhove, Carina Paola; Saad, Silvia; et al.; Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits; Springer; Lipids; 49; 7; 7-2014; 685-6930024-42011558-9307enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-014-3915-6info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s11745-014-3915-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:33Zoai:ri.conicet.gov.ar:11336/19420instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:33.502CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits
title Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits
spellingShingle Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits
Medina, María Fernanda
Hypercholesterolemia
Arachidonic Acid
Prostaglandins
Rabbit Aorta
Endothelial Dysfunction
Fatty Acids
title_short Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits
title_full Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits
title_fullStr Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits
title_full_unstemmed Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits
title_sort Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits
dc.creator.none.fl_str_mv Medina, María Fernanda
Alberto, Maria Rosa
Sierra, Liliana
Van Nieuwenhove, Carina Paola
Saad, Silvia
Isla, Maria Ines
Jerez, Susana Josefina
author Medina, María Fernanda
author_facet Medina, María Fernanda
Alberto, Maria Rosa
Sierra, Liliana
Van Nieuwenhove, Carina Paola
Saad, Silvia
Isla, Maria Ines
Jerez, Susana Josefina
author_role author
author2 Alberto, Maria Rosa
Sierra, Liliana
Van Nieuwenhove, Carina Paola
Saad, Silvia
Isla, Maria Ines
Jerez, Susana Josefina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Hypercholesterolemia
Arachidonic Acid
Prostaglandins
Rabbit Aorta
Endothelial Dysfunction
Fatty Acids
topic Hypercholesterolemia
Arachidonic Acid
Prostaglandins
Rabbit Aorta
Endothelial Dysfunction
Fatty Acids
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present study evaluatedthe plasma fatty acidlevels and thevascular prostaglandin releaseina rabbit model of early hypercholesterolemia with endothelial dysfunction. Rabbits were fed either a control diet (CD) or a diet containing 1 % cholesterol (HD) for 5-6 weeks. The level of fatty acids was measured in plasma. The levels of prostaglandin and nitric oxide (NO) released from aorta were also determined. Vascular morphology of aorta was characterized by intima and media thickness measurement. The rabbits fed with HD had higher levels of arachidonic acid (ARA) and lower levels of oleic acid (OLA). Linoleic acid (LNA) level was unchanged. PGI2 and NO were diminished and PGF2αlevels, the PGI2/TXA2 ratio and the intima/media ratio were increased in rabbits fed with HD. In conclusion, feeding a HD for a short period increased ARA plasma levels and unbalanced release of vasodilator/vasoconstrictor PG redirecting the pathway to vasoconstrictor metabolite release. These lipid metabolism alterations in addition to the reduced NO levels and the moderate changes in the vascular morphology would contribute to the endothelial dysfunction in this animal model. Therefore, the present findings support the importance ofearly correction or prevention of high cholesterol levels to disruptthe endothelial dysfunction processthat leadsto cardiovascular disease.
Fil: Medina, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Alberto, Maria Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto de Química del Noroeste. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Sierra, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Van Nieuwenhove, Carina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Saad, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto de Química del Noroeste. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Jerez, Susana Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
description The present study evaluatedthe plasma fatty acidlevels and thevascular prostaglandin releaseina rabbit model of early hypercholesterolemia with endothelial dysfunction. Rabbits were fed either a control diet (CD) or a diet containing 1 % cholesterol (HD) for 5-6 weeks. The level of fatty acids was measured in plasma. The levels of prostaglandin and nitric oxide (NO) released from aorta were also determined. Vascular morphology of aorta was characterized by intima and media thickness measurement. The rabbits fed with HD had higher levels of arachidonic acid (ARA) and lower levels of oleic acid (OLA). Linoleic acid (LNA) level was unchanged. PGI2 and NO were diminished and PGF2αlevels, the PGI2/TXA2 ratio and the intima/media ratio were increased in rabbits fed with HD. In conclusion, feeding a HD for a short period increased ARA plasma levels and unbalanced release of vasodilator/vasoconstrictor PG redirecting the pathway to vasoconstrictor metabolite release. These lipid metabolism alterations in addition to the reduced NO levels and the moderate changes in the vascular morphology would contribute to the endothelial dysfunction in this animal model. Therefore, the present findings support the importance ofearly correction or prevention of high cholesterol levels to disruptthe endothelial dysfunction processthat leadsto cardiovascular disease.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://dx.doi.org/10.1007/s11745-014-3915-6
https://link.springer.com/article/10.1007/s11745-014-3915-6
http://hdl.handle.net/11336/19420
Medina, María Fernanda; Alberto, Maria Rosa; Sierra, Liliana; Van Nieuwenhove, Carina Paola; Saad, Silvia; et al.; Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits; Springer; Lipids; 49; 7; 7-2014; 685-693
0024-4201
1558-9307
url http://dx.doi.org/10.1007/s11745-014-3915-6
https://link.springer.com/article/10.1007/s11745-014-3915-6
http://hdl.handle.net/11336/19420
identifier_str_mv Medina, María Fernanda; Alberto, Maria Rosa; Sierra, Liliana; Van Nieuwenhove, Carina Paola; Saad, Silvia; et al.; Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits; Springer; Lipids; 49; 7; 7-2014; 685-693
0024-4201
1558-9307
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-014-3915-6
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s11745-014-3915-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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