8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity

Autores
Boztepe, Tugce; Scioli Montoto, Sebastián; Ruiz, María Esperanza; Alvarez, Valeria Alejandra; Castro, Guillermo Raul; Leon, Ignacio Esteban
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Every year the incidence of cancer and the death rate are increasing worldwide. The drug 8-hydroxyquinoline platinum(II) [PtCl(8-O-quinoline)(dmso)] (8HQ-Pt) has been identified as a promising antitumor complex. Nanostructured lipid carriers (NLC) are second-generation drug nanocarrier systems that have superior advantages over other kinds of colloidal carrier systems. 8HQ-Pt compound loaded NLC formulations of cetyl esters (SS) were synthesized via ultrasonication in the presence of two different liquid oils: capric triglyceride, or olive oil. The physicochemical and microscopic characterizations of NLC were analyzed via dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and X-ray diffraction analysis (XRD). In vitro drug release and cytotoxicity, cell uptake and apoptosis assays against the human colon cancer cell line HT-29 were investigated. The results showed that NLCs indicated a narrow size distribution and a mean particle diameter in the range of 136?159 nm. The thermal characteristic analysis confirmed the stability of NLCs up to 185 °C. Encapsulation efficiencies of the 8HQ-Pt compound in NLCs were about 80% and the 8HQ-Pt compound in the formulations showed a controlled release profile during 72 h. The release profiles of these two different formulations and the antitumor effect on the HT-29 cell line were compared with those of the free 8HQ-Pt compound. The cellular uptake of two different NLC groups was proved by fluorescence microscopy and the presence of capric triglyceride liquid oil in the formulation increased the intracellular drug delivery capacity when compared with olive oil.
Fil: Boztepe, Tugce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Scioli Montoto, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina
Fil: Ruiz, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina
Fil: Alvarez, Valeria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Materia
8-HYDROXYQUINOLINE PLATINUM
NANOSTRUCTURED LIPID CARRIERS
ANTITUMOR
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/130044
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activityBoztepe, TugceScioli Montoto, SebastiánRuiz, María EsperanzaAlvarez, Valeria AlejandraCastro, Guillermo RaulLeon, Ignacio Esteban8-HYDROXYQUINOLINE PLATINUMNANOSTRUCTURED LIPID CARRIERSANTITUMORhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Every year the incidence of cancer and the death rate are increasing worldwide. The drug 8-hydroxyquinoline platinum(II) [PtCl(8-O-quinoline)(dmso)] (8HQ-Pt) has been identified as a promising antitumor complex. Nanostructured lipid carriers (NLC) are second-generation drug nanocarrier systems that have superior advantages over other kinds of colloidal carrier systems. 8HQ-Pt compound loaded NLC formulations of cetyl esters (SS) were synthesized via ultrasonication in the presence of two different liquid oils: capric triglyceride, or olive oil. The physicochemical and microscopic characterizations of NLC were analyzed via dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and X-ray diffraction analysis (XRD). In vitro drug release and cytotoxicity, cell uptake and apoptosis assays against the human colon cancer cell line HT-29 were investigated. The results showed that NLCs indicated a narrow size distribution and a mean particle diameter in the range of 136?159 nm. The thermal characteristic analysis confirmed the stability of NLCs up to 185 °C. Encapsulation efficiencies of the 8HQ-Pt compound in NLCs were about 80% and the 8HQ-Pt compound in the formulations showed a controlled release profile during 72 h. The release profiles of these two different formulations and the antitumor effect on the HT-29 cell line were compared with those of the free 8HQ-Pt compound. The cellular uptake of two different NLC groups was proved by fluorescence microscopy and the presence of capric triglyceride liquid oil in the formulation increased the intracellular drug delivery capacity when compared with olive oil.Fil: Boztepe, Tugce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Scioli Montoto, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; ArgentinaFil: Ruiz, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; ArgentinaFil: Alvarez, Valeria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaRoyal Society of Chemistry2021-01-08info:eu-repo/date/embargoEnd/2021-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/130044Boztepe, Tugce; Scioli Montoto, Sebastián; Ruiz, María Esperanza; Alvarez, Valeria Alejandra; Castro, Guillermo Raul; et al.; 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity; Royal Society of Chemistry; New Journal of Chemistry; 45; 2; 8-1-2021; 821-8301144-0546CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=D0NJ03940Cinfo:eu-repo/semantics/altIdentifier/doi//10.1039/D0NJ03940Cinfo:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:18:50Zoai:ri.conicet.gov.ar:11336/130044instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:18:50.508CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity
title 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity
spellingShingle 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity
Boztepe, Tugce
8-HYDROXYQUINOLINE PLATINUM
NANOSTRUCTURED LIPID CARRIERS
ANTITUMOR
title_short 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity
title_full 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity
title_fullStr 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity
title_full_unstemmed 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity
title_sort 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity
dc.creator.none.fl_str_mv Boztepe, Tugce
Scioli Montoto, Sebastián
Ruiz, María Esperanza
Alvarez, Valeria Alejandra
Castro, Guillermo Raul
Leon, Ignacio Esteban
author Boztepe, Tugce
author_facet Boztepe, Tugce
Scioli Montoto, Sebastián
Ruiz, María Esperanza
Alvarez, Valeria Alejandra
Castro, Guillermo Raul
Leon, Ignacio Esteban
author_role author
author2 Scioli Montoto, Sebastián
Ruiz, María Esperanza
Alvarez, Valeria Alejandra
Castro, Guillermo Raul
Leon, Ignacio Esteban
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv 8-HYDROXYQUINOLINE PLATINUM
NANOSTRUCTURED LIPID CARRIERS
ANTITUMOR
topic 8-HYDROXYQUINOLINE PLATINUM
NANOSTRUCTURED LIPID CARRIERS
ANTITUMOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Every year the incidence of cancer and the death rate are increasing worldwide. The drug 8-hydroxyquinoline platinum(II) [PtCl(8-O-quinoline)(dmso)] (8HQ-Pt) has been identified as a promising antitumor complex. Nanostructured lipid carriers (NLC) are second-generation drug nanocarrier systems that have superior advantages over other kinds of colloidal carrier systems. 8HQ-Pt compound loaded NLC formulations of cetyl esters (SS) were synthesized via ultrasonication in the presence of two different liquid oils: capric triglyceride, or olive oil. The physicochemical and microscopic characterizations of NLC were analyzed via dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and X-ray diffraction analysis (XRD). In vitro drug release and cytotoxicity, cell uptake and apoptosis assays against the human colon cancer cell line HT-29 were investigated. The results showed that NLCs indicated a narrow size distribution and a mean particle diameter in the range of 136?159 nm. The thermal characteristic analysis confirmed the stability of NLCs up to 185 °C. Encapsulation efficiencies of the 8HQ-Pt compound in NLCs were about 80% and the 8HQ-Pt compound in the formulations showed a controlled release profile during 72 h. The release profiles of these two different formulations and the antitumor effect on the HT-29 cell line were compared with those of the free 8HQ-Pt compound. The cellular uptake of two different NLC groups was proved by fluorescence microscopy and the presence of capric triglyceride liquid oil in the formulation increased the intracellular drug delivery capacity when compared with olive oil.
Fil: Boztepe, Tugce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Scioli Montoto, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina
Fil: Ruiz, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina
Fil: Alvarez, Valeria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
description Every year the incidence of cancer and the death rate are increasing worldwide. The drug 8-hydroxyquinoline platinum(II) [PtCl(8-O-quinoline)(dmso)] (8HQ-Pt) has been identified as a promising antitumor complex. Nanostructured lipid carriers (NLC) are second-generation drug nanocarrier systems that have superior advantages over other kinds of colloidal carrier systems. 8HQ-Pt compound loaded NLC formulations of cetyl esters (SS) were synthesized via ultrasonication in the presence of two different liquid oils: capric triglyceride, or olive oil. The physicochemical and microscopic characterizations of NLC were analyzed via dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and X-ray diffraction analysis (XRD). In vitro drug release and cytotoxicity, cell uptake and apoptosis assays against the human colon cancer cell line HT-29 were investigated. The results showed that NLCs indicated a narrow size distribution and a mean particle diameter in the range of 136?159 nm. The thermal characteristic analysis confirmed the stability of NLCs up to 185 °C. Encapsulation efficiencies of the 8HQ-Pt compound in NLCs were about 80% and the 8HQ-Pt compound in the formulations showed a controlled release profile during 72 h. The release profiles of these two different formulations and the antitumor effect on the HT-29 cell line were compared with those of the free 8HQ-Pt compound. The cellular uptake of two different NLC groups was proved by fluorescence microscopy and the presence of capric triglyceride liquid oil in the formulation increased the intracellular drug delivery capacity when compared with olive oil.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-08
info:eu-repo/date/embargoEnd/2021-07-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/130044
Boztepe, Tugce; Scioli Montoto, Sebastián; Ruiz, María Esperanza; Alvarez, Valeria Alejandra; Castro, Guillermo Raul; et al.; 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity; Royal Society of Chemistry; New Journal of Chemistry; 45; 2; 8-1-2021; 821-830
1144-0546
CONICET Digital
CONICET
url http://hdl.handle.net/11336/130044
identifier_str_mv Boztepe, Tugce; Scioli Montoto, Sebastián; Ruiz, María Esperanza; Alvarez, Valeria Alejandra; Castro, Guillermo Raul; et al.; 8-Hydroxyquinoline platinum(ii) loaded nanostructured lipid carriers: synthesis, physicochemical characterization and evaluation of antitumor activity; Royal Society of Chemistry; New Journal of Chemistry; 45; 2; 8-1-2021; 821-830
1144-0546
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=D0NJ03940C
info:eu-repo/semantics/altIdentifier/doi//10.1039/D0NJ03940C
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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