Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina

Autores
Fernandez, Daniel; Irino, K.; Sanz, M.; Padola, Nora Lía; Parma, A. E.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To feno-genotypically characterize the Shiga toxin-producing Escherichia coli (STEC) population in Argentinean dairy cows. Methods and Results: From 540 STEC positive samples, 170 isolates were analyzed by multiplex PCR and serotyping. Of these, 11% carried stx1, 52% stx2 and 37% stx1 ⁄ stx2. The ehxA, saa and eae were detected in 77%, 66% and 3%, respectively. Thirty-five per cent of strains harboured the profile stx1, stx2, saa, ehxA and 29% stx2, saa, ehxA. One hundred and fifty-six strains were associated with 29 different O serogroups, and 19 H antigens were distributed among 157 strains. STEC O113:H21, O130:H11 and O178:H19 were the most frequently found serotypes. The STEC O157:H7 were detected in low rate and corresponded to the stx2+, eae+, ehxA+ virulence pattern. Conclusions: We detected a diversity of STEC strains in dairy cattle from Argentina, most of them carrying genes linked to human disease. Significance and Impact of the study: The non-O157 STEC serotypes described in this study are associated worldwide with disease in humans and represent a risk for the public health. For this, any microbiological control in dairy farms should be targeted not only to the search of O157:H7 serotype.
Fil: Fernandez, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina
Fil: Irino, K.. Instituto Adolfo Lutz; Brasil
Fil: Sanz, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Parma, A. E.. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Materia
DAIRY COWS
E. COLI O157:H7
SHIGA TOXIN
STEC
VIRULENCE GENES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242942
Acceder
id CONICETDig_81545337760db34f6ce05057271a37ad
oai_identifier_str oai:ri.conicet.gov.ar:11336/242942
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in ArgentinaFernandez, DanielIrino, K.Sanz, M.Padola, Nora LíaParma, A. E.DAIRY COWSE. COLI O157:H7SHIGA TOXINSTECVIRULENCE GENEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Aims: To feno-genotypically characterize the Shiga toxin-producing Escherichia coli (STEC) population in Argentinean dairy cows. Methods and Results: From 540 STEC positive samples, 170 isolates were analyzed by multiplex PCR and serotyping. Of these, 11% carried stx1, 52% stx2 and 37% stx1 ⁄ stx2. The ehxA, saa and eae were detected in 77%, 66% and 3%, respectively. Thirty-five per cent of strains harboured the profile stx1, stx2, saa, ehxA and 29% stx2, saa, ehxA. One hundred and fifty-six strains were associated with 29 different O serogroups, and 19 H antigens were distributed among 157 strains. STEC O113:H21, O130:H11 and O178:H19 were the most frequently found serotypes. The STEC O157:H7 were detected in low rate and corresponded to the stx2+, eae+, ehxA+ virulence pattern. Conclusions: We detected a diversity of STEC strains in dairy cattle from Argentina, most of them carrying genes linked to human disease. Significance and Impact of the study: The non-O157 STEC serotypes described in this study are associated worldwide with disease in humans and represent a risk for the public health. For this, any microbiological control in dairy farms should be targeted not only to the search of O157:H7 serotype.Fil: Fernandez, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; ArgentinaFil: Irino, K.. Instituto Adolfo Lutz; BrasilFil: Sanz, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Parma, A. E.. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaWiley Blackwell Publishing, Inc2010-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242942Fernandez, Daniel; Irino, K.; Sanz, M.; Padola, Nora Lía; Parma, A. E.; Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 51; 4; 7-2010; 377-3820266-82541472-765XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2010.02904.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:13Zoai:ri.conicet.gov.ar:11336/242942instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:14.218CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina
title Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina
spellingShingle Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina
Fernandez, Daniel
DAIRY COWS
E. COLI O157:H7
SHIGA TOXIN
STEC
VIRULENCE GENES
title_short Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina
title_full Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina
title_fullStr Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina
title_full_unstemmed Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina
title_sort Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina
dc.creator.none.fl_str_mv Fernandez, Daniel
Irino, K.
Sanz, M.
Padola, Nora Lía
Parma, A. E.
author Fernandez, Daniel
author_facet Fernandez, Daniel
Irino, K.
Sanz, M.
Padola, Nora Lía
Parma, A. E.
author_role author
author2 Irino, K.
Sanz, M.
Padola, Nora Lía
Parma, A. E.
author2_role author
author
author
author
dc.subject.none.fl_str_mv DAIRY COWS
E. COLI O157:H7
SHIGA TOXIN
STEC
VIRULENCE GENES
topic DAIRY COWS
E. COLI O157:H7
SHIGA TOXIN
STEC
VIRULENCE GENES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Aims: To feno-genotypically characterize the Shiga toxin-producing Escherichia coli (STEC) population in Argentinean dairy cows. Methods and Results: From 540 STEC positive samples, 170 isolates were analyzed by multiplex PCR and serotyping. Of these, 11% carried stx1, 52% stx2 and 37% stx1 ⁄ stx2. The ehxA, saa and eae were detected in 77%, 66% and 3%, respectively. Thirty-five per cent of strains harboured the profile stx1, stx2, saa, ehxA and 29% stx2, saa, ehxA. One hundred and fifty-six strains were associated with 29 different O serogroups, and 19 H antigens were distributed among 157 strains. STEC O113:H21, O130:H11 and O178:H19 were the most frequently found serotypes. The STEC O157:H7 were detected in low rate and corresponded to the stx2+, eae+, ehxA+ virulence pattern. Conclusions: We detected a diversity of STEC strains in dairy cattle from Argentina, most of them carrying genes linked to human disease. Significance and Impact of the study: The non-O157 STEC serotypes described in this study are associated worldwide with disease in humans and represent a risk for the public health. For this, any microbiological control in dairy farms should be targeted not only to the search of O157:H7 serotype.
Fil: Fernandez, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina
Fil: Irino, K.. Instituto Adolfo Lutz; Brasil
Fil: Sanz, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Parma, A. E.. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
description Aims: To feno-genotypically characterize the Shiga toxin-producing Escherichia coli (STEC) population in Argentinean dairy cows. Methods and Results: From 540 STEC positive samples, 170 isolates were analyzed by multiplex PCR and serotyping. Of these, 11% carried stx1, 52% stx2 and 37% stx1 ⁄ stx2. The ehxA, saa and eae were detected in 77%, 66% and 3%, respectively. Thirty-five per cent of strains harboured the profile stx1, stx2, saa, ehxA and 29% stx2, saa, ehxA. One hundred and fifty-six strains were associated with 29 different O serogroups, and 19 H antigens were distributed among 157 strains. STEC O113:H21, O130:H11 and O178:H19 were the most frequently found serotypes. The STEC O157:H7 were detected in low rate and corresponded to the stx2+, eae+, ehxA+ virulence pattern. Conclusions: We detected a diversity of STEC strains in dairy cattle from Argentina, most of them carrying genes linked to human disease. Significance and Impact of the study: The non-O157 STEC serotypes described in this study are associated worldwide with disease in humans and represent a risk for the public health. For this, any microbiological control in dairy farms should be targeted not only to the search of O157:H7 serotype.
publishDate 2010
dc.date.none.fl_str_mv 2010-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242942
Fernandez, Daniel; Irino, K.; Sanz, M.; Padola, Nora Lía; Parma, A. E.; Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 51; 4; 7-2010; 377-382
0266-8254
1472-765X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242942
identifier_str_mv Fernandez, Daniel; Irino, K.; Sanz, M.; Padola, Nora Lía; Parma, A. E.; Characterization of Shiga toxin-producing Escherichia coli isolated from dairy cows in Argentina; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 51; 4; 7-2010; 377-382
0266-8254
1472-765X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2010.02904.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269508837834752
score 13.13397

Publicaciones similares