Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex

Autores
Papinutti, Víctor Leandro
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Exopolysaccharide (EPS) production by Ganoderma lucidum in response to different culture conditions was studied. Cellulose and glucose, in defined media, resulted in the more efficient enhancers of EPS production among the carbon sources tested. In natural media cultures containing glucose and malt extract exhibited a marked increase (up to 29-fold) respect to defined media. Subsequently, high malt extract and glucose concentrations were tested. G. lucidum produced two fractions of EPS, water-soluble and water-insoluble under these culture conditions. The maximum value (15 g L−1) was reached at 21 days in the medium containing 60 g L−1 malt extract and 40 g L−1 glucose. The incomplete utilization of reducing sugars by the fungus in these media suggested that not only did high malt extract and glucose concentrations play a role in EPS production but also the water activity might be involved. A factorial uniform experimental design to test the effect of malt extract, polyethylene glycol (PEG, as water activity depressor), and initial pH on specific EPS production was applied. G. lucidum showed to be a more efficient specific EPS (mg EPS per g mycelium) producer at pH 3.5 in cultures containing the highest PEG and malt extract concentrations
Fil: Papinutti, Víctor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Materia
Doehlert Factorial Design
Exopolisacaride
Ganoderma Lucidum
Water Activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25554
Acceder
id CONICETDig_813652fc46e3bc6d38c50e2288c3c37a
oai_identifier_str oai:ri.conicet.gov.ar:11336/25554
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complexPapinutti, Víctor LeandroDoehlert Factorial DesignExopolisacarideGanoderma LucidumWater Activityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Exopolysaccharide (EPS) production by Ganoderma lucidum in response to different culture conditions was studied. Cellulose and glucose, in defined media, resulted in the more efficient enhancers of EPS production among the carbon sources tested. In natural media cultures containing glucose and malt extract exhibited a marked increase (up to 29-fold) respect to defined media. Subsequently, high malt extract and glucose concentrations were tested. G. lucidum produced two fractions of EPS, water-soluble and water-insoluble under these culture conditions. The maximum value (15 g L−1) was reached at 21 days in the medium containing 60 g L−1 malt extract and 40 g L−1 glucose. The incomplete utilization of reducing sugars by the fungus in these media suggested that not only did high malt extract and glucose concentrations play a role in EPS production but also the water activity might be involved. A factorial uniform experimental design to test the effect of malt extract, polyethylene glycol (PEG, as water activity depressor), and initial pH on specific EPS production was applied. G. lucidum showed to be a more efficient specific EPS (mg EPS per g mycelium) producer at pH 3.5 in cultures containing the highest PEG and malt extract concentrationsFil: Papinutti, Víctor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaElsevier2009-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25554Papinutti, Víctor Leandro; Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex; Elsevier; Bioresource Technology; 101; 6; 28-10-2009; 1941-19460960-85241873-2976CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960852409013273info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2009.09.076info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:54:21Zoai:ri.conicet.gov.ar:11336/25554instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:54:21.283CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex
title Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex
spellingShingle Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex
Papinutti, Víctor Leandro
Doehlert Factorial Design
Exopolisacaride
Ganoderma Lucidum
Water Activity
title_short Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex
title_full Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex
title_fullStr Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex
title_full_unstemmed Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex
title_sort Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex
dc.creator.none.fl_str_mv Papinutti, Víctor Leandro
author Papinutti, Víctor Leandro
author_facet Papinutti, Víctor Leandro
author_role author
dc.subject.none.fl_str_mv Doehlert Factorial Design
Exopolisacaride
Ganoderma Lucidum
Water Activity
topic Doehlert Factorial Design
Exopolisacaride
Ganoderma Lucidum
Water Activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Exopolysaccharide (EPS) production by Ganoderma lucidum in response to different culture conditions was studied. Cellulose and glucose, in defined media, resulted in the more efficient enhancers of EPS production among the carbon sources tested. In natural media cultures containing glucose and malt extract exhibited a marked increase (up to 29-fold) respect to defined media. Subsequently, high malt extract and glucose concentrations were tested. G. lucidum produced two fractions of EPS, water-soluble and water-insoluble under these culture conditions. The maximum value (15 g L−1) was reached at 21 days in the medium containing 60 g L−1 malt extract and 40 g L−1 glucose. The incomplete utilization of reducing sugars by the fungus in these media suggested that not only did high malt extract and glucose concentrations play a role in EPS production but also the water activity might be involved. A factorial uniform experimental design to test the effect of malt extract, polyethylene glycol (PEG, as water activity depressor), and initial pH on specific EPS production was applied. G. lucidum showed to be a more efficient specific EPS (mg EPS per g mycelium) producer at pH 3.5 in cultures containing the highest PEG and malt extract concentrations
Fil: Papinutti, Víctor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
description Exopolysaccharide (EPS) production by Ganoderma lucidum in response to different culture conditions was studied. Cellulose and glucose, in defined media, resulted in the more efficient enhancers of EPS production among the carbon sources tested. In natural media cultures containing glucose and malt extract exhibited a marked increase (up to 29-fold) respect to defined media. Subsequently, high malt extract and glucose concentrations were tested. G. lucidum produced two fractions of EPS, water-soluble and water-insoluble under these culture conditions. The maximum value (15 g L−1) was reached at 21 days in the medium containing 60 g L−1 malt extract and 40 g L−1 glucose. The incomplete utilization of reducing sugars by the fungus in these media suggested that not only did high malt extract and glucose concentrations play a role in EPS production but also the water activity might be involved. A factorial uniform experimental design to test the effect of malt extract, polyethylene glycol (PEG, as water activity depressor), and initial pH on specific EPS production was applied. G. lucidum showed to be a more efficient specific EPS (mg EPS per g mycelium) producer at pH 3.5 in cultures containing the highest PEG and malt extract concentrations
publishDate 2009
dc.date.none.fl_str_mv 2009-10-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25554
Papinutti, Víctor Leandro; Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex; Elsevier; Bioresource Technology; 101; 6; 28-10-2009; 1941-1946
0960-8524
1873-2976
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25554
identifier_str_mv Papinutti, Víctor Leandro; Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex; Elsevier; Bioresource Technology; 101; 6; 28-10-2009; 1941-1946
0960-8524
1873-2976
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960852409013273
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2009.09.076
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083075366191104
score 13.22299

Publicaciones similares