Dynamics and functional relevance of ribonucleoproteic membrane-less organelles

Autores
Boccaccio, Graciela Lidia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The dynamic formation of stress granules (SGs), processing bodies (PBs) and related RNA membrane-less organelles, regulates diverse cellular processes, including the coordination of functionally linked messengers, and the translational regulation at the synapse among others. The formation of these cellular bodies is governed by liquid-liquid phase separation (LLPS) processes, and their dysregulation may provoke pathogenic aggregation. LLPS in vitro depends on the thermal diffusion of macromolecules, which is limited inside cells, where the condensation and dissolution of membrane-less organelles (MLOs) would be helped by energy-driven processes. We found that the active transport by the retrograde motor dynein helps SG assembly, whereas the anterograde motor kinesin mediates SG dissolution, and a tug of war between these molecular motors allows transient SG formation. As in the case of PBs, SGs contain repressed mRNAs but are not required for their silencing, and the contribution of SGs to the protective response triggered upon stress remains elusive. In addition to SGs and PBs, several RNA granules and related MLOs are present in neurons. We found that distinct subsets of PBs and additional RNA bodies located at dendrites and synapses respond selectively to specific synaptic stimuli, which promote their rapid assembly or disassembly, thus controlling the release of bound mRNAs. This modulates the local transcriptome and allows fine-tuning of the translation at the post-synapse. More recently, we focused on Smaug MLOs. Smaug orthologs are highly conserved in the animal kingdom and recognize a wide variety of stem-loops termed Smaug Recognition Elements (SREs), which are present in a large number of mRNAs including nuclear transcripts that encode mitochondrial enzymes. We performed time lapse confocal microscopy and found that Smaug1 MLOs are highly motile and frequently contact mitochondria, speculatively coordinating the transport and/or the translation of nuclear-encoded mRNAs at the mitochondrial periphery.
Fil: Boccaccio, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
SALTA
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
STRESS GRANULE
PROCESSING BODIES
SMAUG
TRANSLATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/165089

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network_name_str CONICET Digital (CONICET)
spelling Dynamics and functional relevance of ribonucleoproteic membrane-less organellesBoccaccio, Graciela LidiaSTRESS GRANULEPROCESSING BODIESSMAUGTRANSLATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The dynamic formation of stress granules (SGs), processing bodies (PBs) and related RNA membrane-less organelles, regulates diverse cellular processes, including the coordination of functionally linked messengers, and the translational regulation at the synapse among others. The formation of these cellular bodies is governed by liquid-liquid phase separation (LLPS) processes, and their dysregulation may provoke pathogenic aggregation. LLPS in vitro depends on the thermal diffusion of macromolecules, which is limited inside cells, where the condensation and dissolution of membrane-less organelles (MLOs) would be helped by energy-driven processes. We found that the active transport by the retrograde motor dynein helps SG assembly, whereas the anterograde motor kinesin mediates SG dissolution, and a tug of war between these molecular motors allows transient SG formation. As in the case of PBs, SGs contain repressed mRNAs but are not required for their silencing, and the contribution of SGs to the protective response triggered upon stress remains elusive. In addition to SGs and PBs, several RNA granules and related MLOs are present in neurons. We found that distinct subsets of PBs and additional RNA bodies located at dendrites and synapses respond selectively to specific synaptic stimuli, which promote their rapid assembly or disassembly, thus controlling the release of bound mRNAs. This modulates the local transcriptome and allows fine-tuning of the translation at the post-synapse. More recently, we focused on Smaug MLOs. Smaug orthologs are highly conserved in the animal kingdom and recognize a wide variety of stem-loops termed Smaug Recognition Elements (SREs), which are present in a large number of mRNAs including nuclear transcripts that encode mitochondrial enzymes. We performed time lapse confocal microscopy and found that Smaug1 MLOs are highly motile and frequently contact mitochondria, speculatively coordinating the transport and/or the translation of nuclear-encoded mRNAs at the mitochondrial periphery.Fil: Boccaccio, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaThe LV Annual SAIB Meeting and XIV PABMB CongressSALTAArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/165089Dynamics and functional relevance of ribonucleoproteic membrane-less organelles; The LV Annual SAIB Meeting and XIV PABMB Congress; SALTA; Argentina; 2019; 1-30327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:06Zoai:ri.conicet.gov.ar:11336/165089instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:06.781CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dynamics and functional relevance of ribonucleoproteic membrane-less organelles
title Dynamics and functional relevance of ribonucleoproteic membrane-less organelles
spellingShingle Dynamics and functional relevance of ribonucleoproteic membrane-less organelles
Boccaccio, Graciela Lidia
STRESS GRANULE
PROCESSING BODIES
SMAUG
TRANSLATION
title_short Dynamics and functional relevance of ribonucleoproteic membrane-less organelles
title_full Dynamics and functional relevance of ribonucleoproteic membrane-less organelles
title_fullStr Dynamics and functional relevance of ribonucleoproteic membrane-less organelles
title_full_unstemmed Dynamics and functional relevance of ribonucleoproteic membrane-less organelles
title_sort Dynamics and functional relevance of ribonucleoproteic membrane-less organelles
dc.creator.none.fl_str_mv Boccaccio, Graciela Lidia
author Boccaccio, Graciela Lidia
author_facet Boccaccio, Graciela Lidia
author_role author
dc.subject.none.fl_str_mv STRESS GRANULE
PROCESSING BODIES
SMAUG
TRANSLATION
topic STRESS GRANULE
PROCESSING BODIES
SMAUG
TRANSLATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The dynamic formation of stress granules (SGs), processing bodies (PBs) and related RNA membrane-less organelles, regulates diverse cellular processes, including the coordination of functionally linked messengers, and the translational regulation at the synapse among others. The formation of these cellular bodies is governed by liquid-liquid phase separation (LLPS) processes, and their dysregulation may provoke pathogenic aggregation. LLPS in vitro depends on the thermal diffusion of macromolecules, which is limited inside cells, where the condensation and dissolution of membrane-less organelles (MLOs) would be helped by energy-driven processes. We found that the active transport by the retrograde motor dynein helps SG assembly, whereas the anterograde motor kinesin mediates SG dissolution, and a tug of war between these molecular motors allows transient SG formation. As in the case of PBs, SGs contain repressed mRNAs but are not required for their silencing, and the contribution of SGs to the protective response triggered upon stress remains elusive. In addition to SGs and PBs, several RNA granules and related MLOs are present in neurons. We found that distinct subsets of PBs and additional RNA bodies located at dendrites and synapses respond selectively to specific synaptic stimuli, which promote their rapid assembly or disassembly, thus controlling the release of bound mRNAs. This modulates the local transcriptome and allows fine-tuning of the translation at the post-synapse. More recently, we focused on Smaug MLOs. Smaug orthologs are highly conserved in the animal kingdom and recognize a wide variety of stem-loops termed Smaug Recognition Elements (SREs), which are present in a large number of mRNAs including nuclear transcripts that encode mitochondrial enzymes. We performed time lapse confocal microscopy and found that Smaug1 MLOs are highly motile and frequently contact mitochondria, speculatively coordinating the transport and/or the translation of nuclear-encoded mRNAs at the mitochondrial periphery.
Fil: Boccaccio, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
SALTA
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description The dynamic formation of stress granules (SGs), processing bodies (PBs) and related RNA membrane-less organelles, regulates diverse cellular processes, including the coordination of functionally linked messengers, and the translational regulation at the synapse among others. The formation of these cellular bodies is governed by liquid-liquid phase separation (LLPS) processes, and their dysregulation may provoke pathogenic aggregation. LLPS in vitro depends on the thermal diffusion of macromolecules, which is limited inside cells, where the condensation and dissolution of membrane-less organelles (MLOs) would be helped by energy-driven processes. We found that the active transport by the retrograde motor dynein helps SG assembly, whereas the anterograde motor kinesin mediates SG dissolution, and a tug of war between these molecular motors allows transient SG formation. As in the case of PBs, SGs contain repressed mRNAs but are not required for their silencing, and the contribution of SGs to the protective response triggered upon stress remains elusive. In addition to SGs and PBs, several RNA granules and related MLOs are present in neurons. We found that distinct subsets of PBs and additional RNA bodies located at dendrites and synapses respond selectively to specific synaptic stimuli, which promote their rapid assembly or disassembly, thus controlling the release of bound mRNAs. This modulates the local transcriptome and allows fine-tuning of the translation at the post-synapse. More recently, we focused on Smaug MLOs. Smaug orthologs are highly conserved in the animal kingdom and recognize a wide variety of stem-loops termed Smaug Recognition Elements (SREs), which are present in a large number of mRNAs including nuclear transcripts that encode mitochondrial enzymes. We performed time lapse confocal microscopy and found that Smaug1 MLOs are highly motile and frequently contact mitochondria, speculatively coordinating the transport and/or the translation of nuclear-encoded mRNAs at the mitochondrial periphery.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/165089
Dynamics and functional relevance of ribonucleoproteic membrane-less organelles; The LV Annual SAIB Meeting and XIV PABMB Congress; SALTA; Argentina; 2019; 1-3
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/165089
identifier_str_mv Dynamics and functional relevance of ribonucleoproteic membrane-less organelles; The LV Annual SAIB Meeting and XIV PABMB Congress; SALTA; Argentina; 2019; 1-3
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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