Dynamics and functional relevance of ribonucleoproteic membrane-less organelles
- Autores
- Boccaccio, Graciela Lidia
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The dynamic formation of stress granules (SGs), processing bodies (PBs) and related RNA membrane-less organelles, regulates diverse cellular processes, including the coordination of functionally linked messengers, and the translational regulation at the synapse among others. The formation of these cellular bodies is governed by liquid-liquid phase separation (LLPS) processes, and their dysregulation may provoke pathogenic aggregation. LLPS in vitro depends on the thermal diffusion of macromolecules, which is limited inside cells, where the condensation and dissolution of membrane-less organelles (MLOs) would be helped by energy-driven processes. We found that the active transport by the retrograde motor dynein helps SG assembly, whereas the anterograde motor kinesin mediates SG dissolution, and a tug of war between these molecular motors allows transient SG formation. As in the case of PBs, SGs contain repressed mRNAs but are not required for their silencing, and the contribution of SGs to the protective response triggered upon stress remains elusive. In addition to SGs and PBs, several RNA granules and related MLOs are present in neurons. We found that distinct subsets of PBs and additional RNA bodies located at dendrites and synapses respond selectively to specific synaptic stimuli, which promote their rapid assembly or disassembly, thus controlling the release of bound mRNAs. This modulates the local transcriptome and allows fine-tuning of the translation at the post-synapse. More recently, we focused on Smaug MLOs. Smaug orthologs are highly conserved in the animal kingdom and recognize a wide variety of stem-loops termed Smaug Recognition Elements (SREs), which are present in a large number of mRNAs including nuclear transcripts that encode mitochondrial enzymes. We performed time lapse confocal microscopy and found that Smaug1 MLOs are highly motile and frequently contact mitochondria, speculatively coordinating the transport and/or the translation of nuclear-encoded mRNAs at the mitochondrial periphery.
Fil: Boccaccio, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
SALTA
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular - Materia
-
STRESS GRANULE
PROCESSING BODIES
SMAUG
TRANSLATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/165089
Ver los metadatos del registro completo
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Dynamics and functional relevance of ribonucleoproteic membrane-less organellesBoccaccio, Graciela LidiaSTRESS GRANULEPROCESSING BODIESSMAUGTRANSLATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The dynamic formation of stress granules (SGs), processing bodies (PBs) and related RNA membrane-less organelles, regulates diverse cellular processes, including the coordination of functionally linked messengers, and the translational regulation at the synapse among others. The formation of these cellular bodies is governed by liquid-liquid phase separation (LLPS) processes, and their dysregulation may provoke pathogenic aggregation. LLPS in vitro depends on the thermal diffusion of macromolecules, which is limited inside cells, where the condensation and dissolution of membrane-less organelles (MLOs) would be helped by energy-driven processes. We found that the active transport by the retrograde motor dynein helps SG assembly, whereas the anterograde motor kinesin mediates SG dissolution, and a tug of war between these molecular motors allows transient SG formation. As in the case of PBs, SGs contain repressed mRNAs but are not required for their silencing, and the contribution of SGs to the protective response triggered upon stress remains elusive. In addition to SGs and PBs, several RNA granules and related MLOs are present in neurons. We found that distinct subsets of PBs and additional RNA bodies located at dendrites and synapses respond selectively to specific synaptic stimuli, which promote their rapid assembly or disassembly, thus controlling the release of bound mRNAs. This modulates the local transcriptome and allows fine-tuning of the translation at the post-synapse. More recently, we focused on Smaug MLOs. Smaug orthologs are highly conserved in the animal kingdom and recognize a wide variety of stem-loops termed Smaug Recognition Elements (SREs), which are present in a large number of mRNAs including nuclear transcripts that encode mitochondrial enzymes. We performed time lapse confocal microscopy and found that Smaug1 MLOs are highly motile and frequently contact mitochondria, speculatively coordinating the transport and/or the translation of nuclear-encoded mRNAs at the mitochondrial periphery.Fil: Boccaccio, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaThe LV Annual SAIB Meeting and XIV PABMB CongressSALTAArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/165089Dynamics and functional relevance of ribonucleoproteic membrane-less organelles; The LV Annual SAIB Meeting and XIV PABMB Congress; SALTA; Argentina; 2019; 1-30327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:06Zoai:ri.conicet.gov.ar:11336/165089instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:06.781CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Dynamics and functional relevance of ribonucleoproteic membrane-less organelles |
title |
Dynamics and functional relevance of ribonucleoproteic membrane-less organelles |
spellingShingle |
Dynamics and functional relevance of ribonucleoproteic membrane-less organelles Boccaccio, Graciela Lidia STRESS GRANULE PROCESSING BODIES SMAUG TRANSLATION |
title_short |
Dynamics and functional relevance of ribonucleoproteic membrane-less organelles |
title_full |
Dynamics and functional relevance of ribonucleoproteic membrane-less organelles |
title_fullStr |
Dynamics and functional relevance of ribonucleoproteic membrane-less organelles |
title_full_unstemmed |
Dynamics and functional relevance of ribonucleoproteic membrane-less organelles |
title_sort |
Dynamics and functional relevance of ribonucleoproteic membrane-less organelles |
dc.creator.none.fl_str_mv |
Boccaccio, Graciela Lidia |
author |
Boccaccio, Graciela Lidia |
author_facet |
Boccaccio, Graciela Lidia |
author_role |
author |
dc.subject.none.fl_str_mv |
STRESS GRANULE PROCESSING BODIES SMAUG TRANSLATION |
topic |
STRESS GRANULE PROCESSING BODIES SMAUG TRANSLATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The dynamic formation of stress granules (SGs), processing bodies (PBs) and related RNA membrane-less organelles, regulates diverse cellular processes, including the coordination of functionally linked messengers, and the translational regulation at the synapse among others. The formation of these cellular bodies is governed by liquid-liquid phase separation (LLPS) processes, and their dysregulation may provoke pathogenic aggregation. LLPS in vitro depends on the thermal diffusion of macromolecules, which is limited inside cells, where the condensation and dissolution of membrane-less organelles (MLOs) would be helped by energy-driven processes. We found that the active transport by the retrograde motor dynein helps SG assembly, whereas the anterograde motor kinesin mediates SG dissolution, and a tug of war between these molecular motors allows transient SG formation. As in the case of PBs, SGs contain repressed mRNAs but are not required for their silencing, and the contribution of SGs to the protective response triggered upon stress remains elusive. In addition to SGs and PBs, several RNA granules and related MLOs are present in neurons. We found that distinct subsets of PBs and additional RNA bodies located at dendrites and synapses respond selectively to specific synaptic stimuli, which promote their rapid assembly or disassembly, thus controlling the release of bound mRNAs. This modulates the local transcriptome and allows fine-tuning of the translation at the post-synapse. More recently, we focused on Smaug MLOs. Smaug orthologs are highly conserved in the animal kingdom and recognize a wide variety of stem-loops termed Smaug Recognition Elements (SREs), which are present in a large number of mRNAs including nuclear transcripts that encode mitochondrial enzymes. We performed time lapse confocal microscopy and found that Smaug1 MLOs are highly motile and frequently contact mitochondria, speculatively coordinating the transport and/or the translation of nuclear-encoded mRNAs at the mitochondrial periphery. Fil: Boccaccio, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina The LV Annual SAIB Meeting and XIV PABMB Congress SALTA Argentina Sociedad Argentina de Investigación en Bioquímica y Biología Molecular |
description |
The dynamic formation of stress granules (SGs), processing bodies (PBs) and related RNA membrane-less organelles, regulates diverse cellular processes, including the coordination of functionally linked messengers, and the translational regulation at the synapse among others. The formation of these cellular bodies is governed by liquid-liquid phase separation (LLPS) processes, and their dysregulation may provoke pathogenic aggregation. LLPS in vitro depends on the thermal diffusion of macromolecules, which is limited inside cells, where the condensation and dissolution of membrane-less organelles (MLOs) would be helped by energy-driven processes. We found that the active transport by the retrograde motor dynein helps SG assembly, whereas the anterograde motor kinesin mediates SG dissolution, and a tug of war between these molecular motors allows transient SG formation. As in the case of PBs, SGs contain repressed mRNAs but are not required for their silencing, and the contribution of SGs to the protective response triggered upon stress remains elusive. In addition to SGs and PBs, several RNA granules and related MLOs are present in neurons. We found that distinct subsets of PBs and additional RNA bodies located at dendrites and synapses respond selectively to specific synaptic stimuli, which promote their rapid assembly or disassembly, thus controlling the release of bound mRNAs. This modulates the local transcriptome and allows fine-tuning of the translation at the post-synapse. More recently, we focused on Smaug MLOs. Smaug orthologs are highly conserved in the animal kingdom and recognize a wide variety of stem-loops termed Smaug Recognition Elements (SREs), which are present in a large number of mRNAs including nuclear transcripts that encode mitochondrial enzymes. We performed time lapse confocal microscopy and found that Smaug1 MLOs are highly motile and frequently contact mitochondria, speculatively coordinating the transport and/or the translation of nuclear-encoded mRNAs at the mitochondrial periphery. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/165089 Dynamics and functional relevance of ribonucleoproteic membrane-less organelles; The LV Annual SAIB Meeting and XIV PABMB Congress; SALTA; Argentina; 2019; 1-3 0327-9545 1667-5746 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/165089 |
identifier_str_mv |
Dynamics and functional relevance of ribonucleoproteic membrane-less organelles; The LV Annual SAIB Meeting and XIV PABMB Congress; SALTA; Argentina; 2019; 1-3 0327-9545 1667-5746 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Tech Science Press |
publisher.none.fl_str_mv |
Tech Science Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613883574091776 |
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13.070432 |