Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer

Autores
May, Maria; Mosto, Julian; Martinez Vazquez, Paula; Gonzalez, Pedro; Rojas, Paola Andrea; Gass, Hugo; Lanari, Claudia Lee Malvina; Molinolo, Alfredo A.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mucinous carcinoma (MBC) is a rare subtype of breast cancer characterized by the production of variable amounts of mucin, with a prognosis better than that of non-mucinous carcinomas (NMBC). The aim of this project was to evaluate the expression of STAT-5, RUNX-2, and FGFR-2 in a cohort of MBC and compare it with that of NMBC using standard immunohistochemistry. STAT-5 and RUNX-2 are two transcription factors with cytoplasmic and/or nuclear localization that have been related to FGFR-2, a tyrosine kinase growth factor receptor that can interact with STAT-5 and with PR in the nuclei of breast cancer cells. Membranous, cytoplasmic, and nuclear staining were evaluated and expressed as the percentage of stained cells (0-100%) multiplied by the staining intensity (0-3), thus obtaining an index ranging from 0 to 300. Nuclear and/or cytoplasmic immunoreactivity of the three proteins were detected in a high number of NMBC. Nuclear FGFR-2 staining correlated with nuclear STAT-5 (p<0.05) and nuclear RUNX-2 (p<0.01) in both tumor types; however MBC had a significant higher expression of nuclear FGFR-2 (p<0.01) and RUNX-2 (p<0.05) than that of NMBC, and displayed positive immunoreactivity of the 3 proteins in 70.8% of the cases. These results suggest that these proteins may have a role in the progression of the mucinous phenotype, in which nuclear STAT-5 may inhibit RUNX-2 prometastatic effect.
Fil: May, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Mosto, Julian.
Fil: Martinez Vazquez, Paula. Hospital General de Pacheco; Argentina. Ministerio de Salud Provincia de Buenos Aires. Hospital Zonal Gral. de Agudos "Magdalena Villegas de Martinez"; Argentina
Fil: Gonzalez, Pedro. Ministerio de Salud Provincia de Buenos Aires. Hospital Zonal Gral. de Agudos "Magdalena Villegas de Martinez"; Argentina
Fil: Rojas, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Gass, Hugo. Ministerio de Salud Provincia de Buenos Aires. Hospital Zonal Gral. de Agudos "Magdalena Villegas de Martinez"; Argentina
Fil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Molinolo, Alfredo A.. University Of California At San Diego; Estados Unidos
Materia
Breast
Stat5
Runx2
Fgfr2
Adenocarcinoma
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8768
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8768
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancerMay, MariaMosto, JulianMartinez Vazquez, PaulaGonzalez, PedroRojas, Paola AndreaGass, HugoLanari, Claudia Lee MalvinaMolinolo, Alfredo A.BreastStat5Runx2Fgfr2Adenocarcinomahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Mucinous carcinoma (MBC) is a rare subtype of breast cancer characterized by the production of variable amounts of mucin, with a prognosis better than that of non-mucinous carcinomas (NMBC). The aim of this project was to evaluate the expression of STAT-5, RUNX-2, and FGFR-2 in a cohort of MBC and compare it with that of NMBC using standard immunohistochemistry. STAT-5 and RUNX-2 are two transcription factors with cytoplasmic and/or nuclear localization that have been related to FGFR-2, a tyrosine kinase growth factor receptor that can interact with STAT-5 and with PR in the nuclei of breast cancer cells. Membranous, cytoplasmic, and nuclear staining were evaluated and expressed as the percentage of stained cells (0-100%) multiplied by the staining intensity (0-3), thus obtaining an index ranging from 0 to 300. Nuclear and/or cytoplasmic immunoreactivity of the three proteins were detected in a high number of NMBC. Nuclear FGFR-2 staining correlated with nuclear STAT-5 (p<0.05) and nuclear RUNX-2 (p<0.01) in both tumor types; however MBC had a significant higher expression of nuclear FGFR-2 (p<0.01) and RUNX-2 (p<0.05) than that of NMBC, and displayed positive immunoreactivity of the 3 proteins in 70.8% of the cases. These results suggest that these proteins may have a role in the progression of the mucinous phenotype, in which nuclear STAT-5 may inhibit RUNX-2 prometastatic effect.Fil: May, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Mosto, Julian.Fil: Martinez Vazquez, Paula. Hospital General de Pacheco; Argentina. Ministerio de Salud Provincia de Buenos Aires. Hospital Zonal Gral. de Agudos "Magdalena Villegas de Martinez"; ArgentinaFil: Gonzalez, Pedro. Ministerio de Salud Provincia de Buenos Aires. Hospital Zonal Gral. de Agudos "Magdalena Villegas de Martinez"; ArgentinaFil: Rojas, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Gass, Hugo. Ministerio de Salud Provincia de Buenos Aires. Hospital Zonal Gral. de Agudos "Magdalena Villegas de Martinez"; ArgentinaFil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Molinolo, Alfredo A.. University Of California At San Diego; Estados UnidosElsevier2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8768May, Maria; Mosto, Julian; Martinez Vazquez, Paula; Gonzalez, Pedro; Rojas, Paola Andrea; et al.; Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer; Elsevier; Experimental And Molecular Pathology; 100; 1; 2-2016; 39-440014-48001096-0945enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014480015002142info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.yexmp.2015.11.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:49Zoai:ri.conicet.gov.ar:11336/8768instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:49.592CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer
title Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer
spellingShingle Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer
May, Maria
Breast
Stat5
Runx2
Fgfr2
Adenocarcinoma
title_short Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer
title_full Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer
title_fullStr Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer
title_full_unstemmed Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer
title_sort Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer
dc.creator.none.fl_str_mv May, Maria
Mosto, Julian
Martinez Vazquez, Paula
Gonzalez, Pedro
Rojas, Paola Andrea
Gass, Hugo
Lanari, Claudia Lee Malvina
Molinolo, Alfredo A.
author May, Maria
author_facet May, Maria
Mosto, Julian
Martinez Vazquez, Paula
Gonzalez, Pedro
Rojas, Paola Andrea
Gass, Hugo
Lanari, Claudia Lee Malvina
Molinolo, Alfredo A.
author_role author
author2 Mosto, Julian
Martinez Vazquez, Paula
Gonzalez, Pedro
Rojas, Paola Andrea
Gass, Hugo
Lanari, Claudia Lee Malvina
Molinolo, Alfredo A.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Breast
Stat5
Runx2
Fgfr2
Adenocarcinoma
topic Breast
Stat5
Runx2
Fgfr2
Adenocarcinoma
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Mucinous carcinoma (MBC) is a rare subtype of breast cancer characterized by the production of variable amounts of mucin, with a prognosis better than that of non-mucinous carcinomas (NMBC). The aim of this project was to evaluate the expression of STAT-5, RUNX-2, and FGFR-2 in a cohort of MBC and compare it with that of NMBC using standard immunohistochemistry. STAT-5 and RUNX-2 are two transcription factors with cytoplasmic and/or nuclear localization that have been related to FGFR-2, a tyrosine kinase growth factor receptor that can interact with STAT-5 and with PR in the nuclei of breast cancer cells. Membranous, cytoplasmic, and nuclear staining were evaluated and expressed as the percentage of stained cells (0-100%) multiplied by the staining intensity (0-3), thus obtaining an index ranging from 0 to 300. Nuclear and/or cytoplasmic immunoreactivity of the three proteins were detected in a high number of NMBC. Nuclear FGFR-2 staining correlated with nuclear STAT-5 (p<0.05) and nuclear RUNX-2 (p<0.01) in both tumor types; however MBC had a significant higher expression of nuclear FGFR-2 (p<0.01) and RUNX-2 (p<0.05) than that of NMBC, and displayed positive immunoreactivity of the 3 proteins in 70.8% of the cases. These results suggest that these proteins may have a role in the progression of the mucinous phenotype, in which nuclear STAT-5 may inhibit RUNX-2 prometastatic effect.
Fil: May, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Mosto, Julian.
Fil: Martinez Vazquez, Paula. Hospital General de Pacheco; Argentina. Ministerio de Salud Provincia de Buenos Aires. Hospital Zonal Gral. de Agudos "Magdalena Villegas de Martinez"; Argentina
Fil: Gonzalez, Pedro. Ministerio de Salud Provincia de Buenos Aires. Hospital Zonal Gral. de Agudos "Magdalena Villegas de Martinez"; Argentina
Fil: Rojas, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Gass, Hugo. Ministerio de Salud Provincia de Buenos Aires. Hospital Zonal Gral. de Agudos "Magdalena Villegas de Martinez"; Argentina
Fil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Molinolo, Alfredo A.. University Of California At San Diego; Estados Unidos
description Mucinous carcinoma (MBC) is a rare subtype of breast cancer characterized by the production of variable amounts of mucin, with a prognosis better than that of non-mucinous carcinomas (NMBC). The aim of this project was to evaluate the expression of STAT-5, RUNX-2, and FGFR-2 in a cohort of MBC and compare it with that of NMBC using standard immunohistochemistry. STAT-5 and RUNX-2 are two transcription factors with cytoplasmic and/or nuclear localization that have been related to FGFR-2, a tyrosine kinase growth factor receptor that can interact with STAT-5 and with PR in the nuclei of breast cancer cells. Membranous, cytoplasmic, and nuclear staining were evaluated and expressed as the percentage of stained cells (0-100%) multiplied by the staining intensity (0-3), thus obtaining an index ranging from 0 to 300. Nuclear and/or cytoplasmic immunoreactivity of the three proteins were detected in a high number of NMBC. Nuclear FGFR-2 staining correlated with nuclear STAT-5 (p<0.05) and nuclear RUNX-2 (p<0.01) in both tumor types; however MBC had a significant higher expression of nuclear FGFR-2 (p<0.01) and RUNX-2 (p<0.05) than that of NMBC, and displayed positive immunoreactivity of the 3 proteins in 70.8% of the cases. These results suggest that these proteins may have a role in the progression of the mucinous phenotype, in which nuclear STAT-5 may inhibit RUNX-2 prometastatic effect.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8768
May, Maria; Mosto, Julian; Martinez Vazquez, Paula; Gonzalez, Pedro; Rojas, Paola Andrea; et al.; Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer; Elsevier; Experimental And Molecular Pathology; 100; 1; 2-2016; 39-44
0014-4800
1096-0945
url http://hdl.handle.net/11336/8768
identifier_str_mv May, Maria; Mosto, Julian; Martinez Vazquez, Paula; Gonzalez, Pedro; Rojas, Paola Andrea; et al.; Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer; Elsevier; Experimental And Molecular Pathology; 100; 1; 2-2016; 39-44
0014-4800
1096-0945
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014480015002142
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.yexmp.2015.11.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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