Inhibitory learning of phototaxis by honey bees in a passive-avoidance task

Autores
Marchal, P.; Villar, María Eugenia; Heiyang, Geng; Arrufat, Patrick; Combe, Maud; Viola, Haydee Ana Maria; Massou, Isabelle; Giurfa, Martín
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Honeybees are a standard model for the study of appetitive learning and memory. Yet, fewer attempts have been performedto characterize aversive learning and memory in this insect and uncover its molecular underpinnings. Here, wetook advantage of the positive phototactic behavior of bees kept away from the hive in a dark environment and establisheda passive-avoidance task in which they had to suppress positive phototaxis. Bees placed in a two-compartment box learned toinhibit spontaneous attraction to a compartment illuminated with blue light by associating and entering into that chamberwith shock delivery. Inhibitory learning resulted in an avoidance memory that could be retrieved 24 h after training andthat was specific to the punished blue light. It was mainly operant but involves a Pavlovian component linking the blue lightQ4 and the shock. Coupling and conditioning with transcriptional analyses in key areas of the brain showed that inhibitorylearning of phototaxis leads to an up-regulation of the dopaminergic receptor gene Amdop1 in the calyces of the mushroombodies, consistently with the role of dopamine signaling in different forms of aversive learning in insects. Our results thusintroduce new perspectives for uncovering further cellular and molecular underpinnings of aversive learning and memoryin bees. Overall, they represent an important step toward comparative learning studies between the appetitive and the aversiveframeworks.
Fil: Marchal, P.. Centre National de la Recherche Scientifique; Francia
Fil: Villar, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Heiyang, Geng. Centre National de la Recherche Scientifique; Francia
Fil: Arrufat, Patrick. Centre National de la Recherche Scientifique; Francia
Fil: Combe, Maud. Centre National de la Recherche Scientifique; Francia
Fil: Viola, Haydee Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Massou, Isabelle. Centre National de la Recherche Scientifique; Francia
Fil: Giurfa, Martín. Centre National de la Recherche Scientifique; Francia
Materia
LONG-TERM MEMROY
PASIVE-AVOIDANCE TASK
HONEYBEE
PHOTOTAXIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/125762
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inhibitory learning of phototaxis by honey bees in a passive-avoidance taskMarchal, P.Villar, María EugeniaHeiyang, GengArrufat, PatrickCombe, MaudViola, Haydee Ana MariaMassou, IsabelleGiurfa, MartínLONG-TERM MEMROYPASIVE-AVOIDANCE TASKHONEYBEEPHOTOTAXIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Honeybees are a standard model for the study of appetitive learning and memory. Yet, fewer attempts have been performedto characterize aversive learning and memory in this insect and uncover its molecular underpinnings. Here, wetook advantage of the positive phototactic behavior of bees kept away from the hive in a dark environment and establisheda passive-avoidance task in which they had to suppress positive phototaxis. Bees placed in a two-compartment box learned toinhibit spontaneous attraction to a compartment illuminated with blue light by associating and entering into that chamberwith shock delivery. Inhibitory learning resulted in an avoidance memory that could be retrieved 24 h after training andthat was specific to the punished blue light. It was mainly operant but involves a Pavlovian component linking the blue lightQ4 and the shock. Coupling and conditioning with transcriptional analyses in key areas of the brain showed that inhibitorylearning of phototaxis leads to an up-regulation of the dopaminergic receptor gene Amdop1 in the calyces of the mushroombodies, consistently with the role of dopamine signaling in different forms of aversive learning in insects. Our results thusintroduce new perspectives for uncovering further cellular and molecular underpinnings of aversive learning and memoryin bees. Overall, they represent an important step toward comparative learning studies between the appetitive and the aversiveframeworks.Fil: Marchal, P.. Centre National de la Recherche Scientifique; FranciaFil: Villar, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Heiyang, Geng. Centre National de la Recherche Scientifique; FranciaFil: Arrufat, Patrick. Centre National de la Recherche Scientifique; FranciaFil: Combe, Maud. Centre National de la Recherche Scientifique; FranciaFil: Viola, Haydee Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Massou, Isabelle. Centre National de la Recherche Scientifique; FranciaFil: Giurfa, Martín. Centre National de la Recherche Scientifique; FranciaCold Spring Harbor Laboratory Press2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125762Marchal, P.; Villar, María Eugenia; Heiyang, Geng; Arrufat, Patrick; Combe, Maud; et al.; Inhibitory learning of phototaxis by honey bees in a passive-avoidance task; Cold Spring Harbor Laboratory Press; Learning & Memory (Cold Spring Harbor, N.Y.); 9-2019; 1-131072-0502CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://learnmem.cshlp.org/content/26/10/412.fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:01:56Zoai:ri.conicet.gov.ar:11336/125762instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:01:57.03CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibitory learning of phototaxis by honey bees in a passive-avoidance task
title Inhibitory learning of phototaxis by honey bees in a passive-avoidance task
spellingShingle Inhibitory learning of phototaxis by honey bees in a passive-avoidance task
Marchal, P.
LONG-TERM MEMROY
PASIVE-AVOIDANCE TASK
HONEYBEE
PHOTOTAXIS
title_short Inhibitory learning of phototaxis by honey bees in a passive-avoidance task
title_full Inhibitory learning of phototaxis by honey bees in a passive-avoidance task
title_fullStr Inhibitory learning of phototaxis by honey bees in a passive-avoidance task
title_full_unstemmed Inhibitory learning of phototaxis by honey bees in a passive-avoidance task
title_sort Inhibitory learning of phototaxis by honey bees in a passive-avoidance task
dc.creator.none.fl_str_mv Marchal, P.
Villar, María Eugenia
Heiyang, Geng
Arrufat, Patrick
Combe, Maud
Viola, Haydee Ana Maria
Massou, Isabelle
Giurfa, Martín
author Marchal, P.
author_facet Marchal, P.
Villar, María Eugenia
Heiyang, Geng
Arrufat, Patrick
Combe, Maud
Viola, Haydee Ana Maria
Massou, Isabelle
Giurfa, Martín
author_role author
author2 Villar, María Eugenia
Heiyang, Geng
Arrufat, Patrick
Combe, Maud
Viola, Haydee Ana Maria
Massou, Isabelle
Giurfa, Martín
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LONG-TERM MEMROY
PASIVE-AVOIDANCE TASK
HONEYBEE
PHOTOTAXIS
topic LONG-TERM MEMROY
PASIVE-AVOIDANCE TASK
HONEYBEE
PHOTOTAXIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Honeybees are a standard model for the study of appetitive learning and memory. Yet, fewer attempts have been performedto characterize aversive learning and memory in this insect and uncover its molecular underpinnings. Here, wetook advantage of the positive phototactic behavior of bees kept away from the hive in a dark environment and establisheda passive-avoidance task in which they had to suppress positive phototaxis. Bees placed in a two-compartment box learned toinhibit spontaneous attraction to a compartment illuminated with blue light by associating and entering into that chamberwith shock delivery. Inhibitory learning resulted in an avoidance memory that could be retrieved 24 h after training andthat was specific to the punished blue light. It was mainly operant but involves a Pavlovian component linking the blue lightQ4 and the shock. Coupling and conditioning with transcriptional analyses in key areas of the brain showed that inhibitorylearning of phototaxis leads to an up-regulation of the dopaminergic receptor gene Amdop1 in the calyces of the mushroombodies, consistently with the role of dopamine signaling in different forms of aversive learning in insects. Our results thusintroduce new perspectives for uncovering further cellular and molecular underpinnings of aversive learning and memoryin bees. Overall, they represent an important step toward comparative learning studies between the appetitive and the aversiveframeworks.
Fil: Marchal, P.. Centre National de la Recherche Scientifique; Francia
Fil: Villar, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Heiyang, Geng. Centre National de la Recherche Scientifique; Francia
Fil: Arrufat, Patrick. Centre National de la Recherche Scientifique; Francia
Fil: Combe, Maud. Centre National de la Recherche Scientifique; Francia
Fil: Viola, Haydee Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Massou, Isabelle. Centre National de la Recherche Scientifique; Francia
Fil: Giurfa, Martín. Centre National de la Recherche Scientifique; Francia
description Honeybees are a standard model for the study of appetitive learning and memory. Yet, fewer attempts have been performedto characterize aversive learning and memory in this insect and uncover its molecular underpinnings. Here, wetook advantage of the positive phototactic behavior of bees kept away from the hive in a dark environment and establisheda passive-avoidance task in which they had to suppress positive phototaxis. Bees placed in a two-compartment box learned toinhibit spontaneous attraction to a compartment illuminated with blue light by associating and entering into that chamberwith shock delivery. Inhibitory learning resulted in an avoidance memory that could be retrieved 24 h after training andthat was specific to the punished blue light. It was mainly operant but involves a Pavlovian component linking the blue lightQ4 and the shock. Coupling and conditioning with transcriptional analyses in key areas of the brain showed that inhibitorylearning of phototaxis leads to an up-regulation of the dopaminergic receptor gene Amdop1 in the calyces of the mushroombodies, consistently with the role of dopamine signaling in different forms of aversive learning in insects. Our results thusintroduce new perspectives for uncovering further cellular and molecular underpinnings of aversive learning and memoryin bees. Overall, they represent an important step toward comparative learning studies between the appetitive and the aversiveframeworks.
publishDate 2019
dc.date.none.fl_str_mv 2019-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/125762
Marchal, P.; Villar, María Eugenia; Heiyang, Geng; Arrufat, Patrick; Combe, Maud; et al.; Inhibitory learning of phototaxis by honey bees in a passive-avoidance task; Cold Spring Harbor Laboratory Press; Learning & Memory (Cold Spring Harbor, N.Y.); 9-2019; 1-13
1072-0502
CONICET Digital
CONICET
url http://hdl.handle.net/11336/125762
identifier_str_mv Marchal, P.; Villar, María Eugenia; Heiyang, Geng; Arrufat, Patrick; Combe, Maud; et al.; Inhibitory learning of phototaxis by honey bees in a passive-avoidance task; Cold Spring Harbor Laboratory Press; Learning & Memory (Cold Spring Harbor, N.Y.); 9-2019; 1-13
1072-0502
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://learnmem.cshlp.org/content/26/10/412.full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083160802066432
score 13.22299

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