Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae

Autores
Chapela, Sebastian; Congost, Christian; Alonso, Manuel; Burgos, Hilda Isabel; Stella, Carlos Alberto
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of resistance to antifungals is a relevant issue during the treatment of infections. Using S. cerevisiae cells, we studied the effect of dichloroacetate (DCA), onthe growth of the microorganism in the presence of fluconazole (FCZ). The results obtained show that DCA (2.0 - 3.0 mg/ml), sensitizes yeast cells to growth inhibition by fluconazole.Results indicate that the external pH reduction produced by DCA decreases the electrochemical proton gradient necessary for the extrusion of toxic compounds to yeastmetabolism. This effect favors a synergism strategy leading to lower drug doses and reducing drugs side effects.
Fil: Chapela, Sebastian. Hospital Británico de Buenos Aires; Argentina
Fil: Congost, Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Alonso, Manuel. Universidad de Buenos Aires. Ciclo Básico Común; Argentina
Fil: Burgos, Hilda Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Stella, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Materia
Dichloroacetate
Yeast
Fluconazole
Resistance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/203049
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiaeChapela, SebastianCongost, ChristianAlonso, ManuelBurgos, Hilda IsabelStella, Carlos AlbertoDichloroacetateYeastFluconazoleResistancehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The development of resistance to antifungals is a relevant issue during the treatment of infections. Using S. cerevisiae cells, we studied the effect of dichloroacetate (DCA), onthe growth of the microorganism in the presence of fluconazole (FCZ). The results obtained show that DCA (2.0 - 3.0 mg/ml), sensitizes yeast cells to growth inhibition by fluconazole.Results indicate that the external pH reduction produced by DCA decreases the electrochemical proton gradient necessary for the extrusion of toxic compounds to yeastmetabolism. This effect favors a synergism strategy leading to lower drug doses and reducing drugs side effects.Fil: Chapela, Sebastian. Hospital Británico de Buenos Aires; ArgentinaFil: Congost, Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Alonso, Manuel. Universidad de Buenos Aires. Ciclo Básico Común; ArgentinaFil: Burgos, Hilda Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Stella, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaJSciMed Central2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/203049Chapela, Sebastian; Congost, Christian; Alonso, Manuel; Burgos, Hilda Isabel; Stella, Carlos Alberto; Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae; JSciMed Central; Journal of Pharmacology & Clinical Toxicology; 9; 1; 4-2022; 1-52333-7079CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jscimedcentral.com/jounal-article-info/Journal-of-Pharmacology-and-Clinical-Toxicology/Synergistic-Effect-of-Dichloroacetate-and-Fluconazole-on-the-Growth-of-Saccharomyces-cerevisiae-10736info:eu-repo/semantics/altIdentifier/doi/10.47739/2333-7079/1162info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:08Zoai:ri.conicet.gov.ar:11336/203049instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:09.101CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae
title Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae
spellingShingle Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae
Chapela, Sebastian
Dichloroacetate
Yeast
Fluconazole
Resistance
title_short Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae
title_full Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae
title_fullStr Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae
title_full_unstemmed Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae
title_sort Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae
dc.creator.none.fl_str_mv Chapela, Sebastian
Congost, Christian
Alonso, Manuel
Burgos, Hilda Isabel
Stella, Carlos Alberto
author Chapela, Sebastian
author_facet Chapela, Sebastian
Congost, Christian
Alonso, Manuel
Burgos, Hilda Isabel
Stella, Carlos Alberto
author_role author
author2 Congost, Christian
Alonso, Manuel
Burgos, Hilda Isabel
Stella, Carlos Alberto
author2_role author
author
author
author
dc.subject.none.fl_str_mv Dichloroacetate
Yeast
Fluconazole
Resistance
topic Dichloroacetate
Yeast
Fluconazole
Resistance
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The development of resistance to antifungals is a relevant issue during the treatment of infections. Using S. cerevisiae cells, we studied the effect of dichloroacetate (DCA), onthe growth of the microorganism in the presence of fluconazole (FCZ). The results obtained show that DCA (2.0 - 3.0 mg/ml), sensitizes yeast cells to growth inhibition by fluconazole.Results indicate that the external pH reduction produced by DCA decreases the electrochemical proton gradient necessary for the extrusion of toxic compounds to yeastmetabolism. This effect favors a synergism strategy leading to lower drug doses and reducing drugs side effects.
Fil: Chapela, Sebastian. Hospital Británico de Buenos Aires; Argentina
Fil: Congost, Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Alonso, Manuel. Universidad de Buenos Aires. Ciclo Básico Común; Argentina
Fil: Burgos, Hilda Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Stella, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
description The development of resistance to antifungals is a relevant issue during the treatment of infections. Using S. cerevisiae cells, we studied the effect of dichloroacetate (DCA), onthe growth of the microorganism in the presence of fluconazole (FCZ). The results obtained show that DCA (2.0 - 3.0 mg/ml), sensitizes yeast cells to growth inhibition by fluconazole.Results indicate that the external pH reduction produced by DCA decreases the electrochemical proton gradient necessary for the extrusion of toxic compounds to yeastmetabolism. This effect favors a synergism strategy leading to lower drug doses and reducing drugs side effects.
publishDate 2022
dc.date.none.fl_str_mv 2022-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/203049
Chapela, Sebastian; Congost, Christian; Alonso, Manuel; Burgos, Hilda Isabel; Stella, Carlos Alberto; Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae; JSciMed Central; Journal of Pharmacology & Clinical Toxicology; 9; 1; 4-2022; 1-5
2333-7079
CONICET Digital
CONICET
url http://hdl.handle.net/11336/203049
identifier_str_mv Chapela, Sebastian; Congost, Christian; Alonso, Manuel; Burgos, Hilda Isabel; Stella, Carlos Alberto; Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae; JSciMed Central; Journal of Pharmacology & Clinical Toxicology; 9; 1; 4-2022; 1-5
2333-7079
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jscimedcentral.com/jounal-article-info/Journal-of-Pharmacology-and-Clinical-Toxicology/Synergistic-Effect-of-Dichloroacetate-and-Fluconazole-on-the-Growth-of-Saccharomyces-cerevisiae-10736
info:eu-repo/semantics/altIdentifier/doi/10.47739/2333-7079/1162
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv JSciMed Central
publisher.none.fl_str_mv JSciMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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