Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling

Autores
Fernandez Gnecco, Gabriela Amancay; Covacevich, Fernanda; Consolo, Verónica Fabiana; Behr, Jan H.; Sommermann, Loreen; Moradtalab, Narges; Maccario, Lorrie; Sørensen, Søren J.; Deubel, Annette; Schellenberg, Ingo; Geistlinger, Joerg; Neumann, Günter; Grosch, Rita; Smalla, Kornelia; Babin, Doreen
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Application of agrochemicals and mechanization enabled increasing agriculturalproductivity yet caused various environmental and soil health-related problems.Agricultural practices affect soil microorganisms, which are the key players of manyecosystem processes. However, less is known about whether this effect differs betweentime points. Therefore, soil was sampled in winter (without crop) and in summer (inthe presence of maize) from a long-term field experiment (LTE) in Bernburg (Germany)managed either under cultivator tillage (CT) or moldboard plow (MP) in combinationwith either intensive nitrogen (N)-fertilization and pesticides (Int) or extensive reducedN-fertilization without fungicides (Ext), respectively. High-throughput sequencing of 16SrRNA gene and fungal ITS2 amplicons showed that changes in the microbial communitycomposition were correlated to differences in soil chemical properties caused by tillagepractice. Microbial communities of soils sampled in winter differed only depending onthe tillage practice while, in summer, also a strong effect of the fertilization intensity wasobserved. A small proportion of microbial taxa was shared between soils from the twosampling times, suggesting the existence of a stable core microbiota at the LTE. Ingeneral, taxa associated with organic matter decomposition (such as Actinobacteria,Bacteroidetes, Rhizopus, and Exophiala) had a higher relative abundance under CT.Among the taxa with significant changes in relative abundances due to different long-termagricultural practices were putative pathogenic (e.g., Gibellulopsis and Gibberella) andbeneficial microbial genera (e.g., Chitinophagaceae, Ferruginibacter, and Minimedusa).In summary, this study suggests that the effects of long-term agricultural managementpractices on the soil microbiota are influenced by the soil sampling time, and this needsto be kept in mind in future studies for the interpretation of field data.
Fil: Fernandez Gnecco, Gabriela Amancay. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Consolo, Verónica Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Behr, Jan H.. Leibniz Institute Of Vegetable And Ornamental Crops (; Alemania
Fil: Sommermann, Loreen. Department Of Agriculture, Ecotrophology And Landscape; Alemania
Fil: Moradtalab, Narges. Department Of Nutritional Crop Physiology, Institute Of; Alemania
Fil: Maccario, Lorrie. Section Of Microbiology, Department Of Biology, Univers; Alemania
Fil: Sørensen, Søren J.. Section Of Microbiology, Department Of Biology, Univers; Alemania
Fil: Deubel, Annette. Department Of Agriculture, Ecotrophology And Landscape; Alemania
Fil: Schellenberg, Ingo. Department Of Agriculture, Ecotrophology And Landscape; Alemania
Fil: Geistlinger, Joerg. Department Of Agriculture, Ecotrophology And Landscape; Alemania
Fil: Neumann, Günter. Department Of Nutritional Crop Physiology, Institute Of; Alemania
Fil: Grosch, Rita. Leibniz Institute Of Vegetable And Ornamental Crops (; Alemania
Fil: Smalla, Kornelia. Julius Kühn Institut Braunschweig; Alemania
Fil: Babin, Doreen. Julius Kühn Institut Braunschweig; Alemania
Materia
TILLAGE PRACTICE
FERTILIZATION INTENSITY
HIGH THROUGHPUT AMPLICON SEQUENCING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/203104
Acceder
id CONICETDig_7ecb228c481209c6c025b435678fc239
oai_identifier_str oai:ri.conicet.gov.ar:11336/203104
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil SamplingFernandez Gnecco, Gabriela AmancayCovacevich, FernandaConsolo, Verónica FabianaBehr, Jan H.Sommermann, LoreenMoradtalab, NargesMaccario, LorrieSørensen, Søren J.Deubel, AnnetteSchellenberg, IngoGeistlinger, JoergNeumann, GünterGrosch, RitaSmalla, KorneliaBabin, DoreenTILLAGE PRACTICEFERTILIZATION INTENSITYHIGH THROUGHPUT AMPLICON SEQUENCINGhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Application of agrochemicals and mechanization enabled increasing agriculturalproductivity yet caused various environmental and soil health-related problems.Agricultural practices affect soil microorganisms, which are the key players of manyecosystem processes. However, less is known about whether this effect differs betweentime points. Therefore, soil was sampled in winter (without crop) and in summer (inthe presence of maize) from a long-term field experiment (LTE) in Bernburg (Germany)managed either under cultivator tillage (CT) or moldboard plow (MP) in combinationwith either intensive nitrogen (N)-fertilization and pesticides (Int) or extensive reducedN-fertilization without fungicides (Ext), respectively. High-throughput sequencing of 16SrRNA gene and fungal ITS2 amplicons showed that changes in the microbial communitycomposition were correlated to differences in soil chemical properties caused by tillagepractice. Microbial communities of soils sampled in winter differed only depending onthe tillage practice while, in summer, also a strong effect of the fertilization intensity wasobserved. A small proportion of microbial taxa was shared between soils from the twosampling times, suggesting the existence of a stable core microbiota at the LTE. Ingeneral, taxa associated with organic matter decomposition (such as Actinobacteria,Bacteroidetes, Rhizopus, and Exophiala) had a higher relative abundance under CT.Among the taxa with significant changes in relative abundances due to different long-termagricultural practices were putative pathogenic (e.g., Gibellulopsis and Gibberella) andbeneficial microbial genera (e.g., Chitinophagaceae, Ferruginibacter, and Minimedusa).In summary, this study suggests that the effects of long-term agricultural managementpractices on the soil microbiota are influenced by the soil sampling time, and this needsto be kept in mind in future studies for the interpretation of field data.Fil: Fernandez Gnecco, Gabriela Amancay. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Consolo, Verónica Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Behr, Jan H.. Leibniz Institute Of Vegetable And Ornamental Crops (; AlemaniaFil: Sommermann, Loreen. Department Of Agriculture, Ecotrophology And Landscape; AlemaniaFil: Moradtalab, Narges. Department Of Nutritional Crop Physiology, Institute Of; AlemaniaFil: Maccario, Lorrie. Section Of Microbiology, Department Of Biology, Univers; AlemaniaFil: Sørensen, Søren J.. Section Of Microbiology, Department Of Biology, Univers; AlemaniaFil: Deubel, Annette. Department Of Agriculture, Ecotrophology And Landscape; AlemaniaFil: Schellenberg, Ingo. Department Of Agriculture, Ecotrophology And Landscape; AlemaniaFil: Geistlinger, Joerg. Department Of Agriculture, Ecotrophology And Landscape; AlemaniaFil: Neumann, Günter. Department Of Nutritional Crop Physiology, Institute Of; AlemaniaFil: Grosch, Rita. Leibniz Institute Of Vegetable And Ornamental Crops (; AlemaniaFil: Smalla, Kornelia. Julius Kühn Institut Braunschweig; AlemaniaFil: Babin, Doreen. Julius Kühn Institut Braunschweig; AlemaniaFrontiers2022-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/203104Fernandez Gnecco, Gabriela Amancay; Covacevich, Fernanda; Consolo, Verónica Fabiana; Behr, Jan H.; Sommermann, Loreen; et al.; Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling; Frontiers; Frontiers in Soil Science; 2; 3-2022; 1-162673-8619CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fsoil.2022.837508/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fsoil.2022.837508info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:57Zoai:ri.conicet.gov.ar:11336/203104instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:58.116CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling
title Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling
spellingShingle Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling
Fernandez Gnecco, Gabriela Amancay
TILLAGE PRACTICE
FERTILIZATION INTENSITY
HIGH THROUGHPUT AMPLICON SEQUENCING
title_short Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling
title_full Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling
title_fullStr Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling
title_full_unstemmed Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling
title_sort Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling
dc.creator.none.fl_str_mv Fernandez Gnecco, Gabriela Amancay
Covacevich, Fernanda
Consolo, Verónica Fabiana
Behr, Jan H.
Sommermann, Loreen
Moradtalab, Narges
Maccario, Lorrie
Sørensen, Søren J.
Deubel, Annette
Schellenberg, Ingo
Geistlinger, Joerg
Neumann, Günter
Grosch, Rita
Smalla, Kornelia
Babin, Doreen
author Fernandez Gnecco, Gabriela Amancay
author_facet Fernandez Gnecco, Gabriela Amancay
Covacevich, Fernanda
Consolo, Verónica Fabiana
Behr, Jan H.
Sommermann, Loreen
Moradtalab, Narges
Maccario, Lorrie
Sørensen, Søren J.
Deubel, Annette
Schellenberg, Ingo
Geistlinger, Joerg
Neumann, Günter
Grosch, Rita
Smalla, Kornelia
Babin, Doreen
author_role author
author2 Covacevich, Fernanda
Consolo, Verónica Fabiana
Behr, Jan H.
Sommermann, Loreen
Moradtalab, Narges
Maccario, Lorrie
Sørensen, Søren J.
Deubel, Annette
Schellenberg, Ingo
Geistlinger, Joerg
Neumann, Günter
Grosch, Rita
Smalla, Kornelia
Babin, Doreen
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TILLAGE PRACTICE
FERTILIZATION INTENSITY
HIGH THROUGHPUT AMPLICON SEQUENCING
topic TILLAGE PRACTICE
FERTILIZATION INTENSITY
HIGH THROUGHPUT AMPLICON SEQUENCING
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Application of agrochemicals and mechanization enabled increasing agriculturalproductivity yet caused various environmental and soil health-related problems.Agricultural practices affect soil microorganisms, which are the key players of manyecosystem processes. However, less is known about whether this effect differs betweentime points. Therefore, soil was sampled in winter (without crop) and in summer (inthe presence of maize) from a long-term field experiment (LTE) in Bernburg (Germany)managed either under cultivator tillage (CT) or moldboard plow (MP) in combinationwith either intensive nitrogen (N)-fertilization and pesticides (Int) or extensive reducedN-fertilization without fungicides (Ext), respectively. High-throughput sequencing of 16SrRNA gene and fungal ITS2 amplicons showed that changes in the microbial communitycomposition were correlated to differences in soil chemical properties caused by tillagepractice. Microbial communities of soils sampled in winter differed only depending onthe tillage practice while, in summer, also a strong effect of the fertilization intensity wasobserved. A small proportion of microbial taxa was shared between soils from the twosampling times, suggesting the existence of a stable core microbiota at the LTE. Ingeneral, taxa associated with organic matter decomposition (such as Actinobacteria,Bacteroidetes, Rhizopus, and Exophiala) had a higher relative abundance under CT.Among the taxa with significant changes in relative abundances due to different long-termagricultural practices were putative pathogenic (e.g., Gibellulopsis and Gibberella) andbeneficial microbial genera (e.g., Chitinophagaceae, Ferruginibacter, and Minimedusa).In summary, this study suggests that the effects of long-term agricultural managementpractices on the soil microbiota are influenced by the soil sampling time, and this needsto be kept in mind in future studies for the interpretation of field data.
Fil: Fernandez Gnecco, Gabriela Amancay. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Consolo, Verónica Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Behr, Jan H.. Leibniz Institute Of Vegetable And Ornamental Crops (; Alemania
Fil: Sommermann, Loreen. Department Of Agriculture, Ecotrophology And Landscape; Alemania
Fil: Moradtalab, Narges. Department Of Nutritional Crop Physiology, Institute Of; Alemania
Fil: Maccario, Lorrie. Section Of Microbiology, Department Of Biology, Univers; Alemania
Fil: Sørensen, Søren J.. Section Of Microbiology, Department Of Biology, Univers; Alemania
Fil: Deubel, Annette. Department Of Agriculture, Ecotrophology And Landscape; Alemania
Fil: Schellenberg, Ingo. Department Of Agriculture, Ecotrophology And Landscape; Alemania
Fil: Geistlinger, Joerg. Department Of Agriculture, Ecotrophology And Landscape; Alemania
Fil: Neumann, Günter. Department Of Nutritional Crop Physiology, Institute Of; Alemania
Fil: Grosch, Rita. Leibniz Institute Of Vegetable And Ornamental Crops (; Alemania
Fil: Smalla, Kornelia. Julius Kühn Institut Braunschweig; Alemania
Fil: Babin, Doreen. Julius Kühn Institut Braunschweig; Alemania
description Application of agrochemicals and mechanization enabled increasing agriculturalproductivity yet caused various environmental and soil health-related problems.Agricultural practices affect soil microorganisms, which are the key players of manyecosystem processes. However, less is known about whether this effect differs betweentime points. Therefore, soil was sampled in winter (without crop) and in summer (inthe presence of maize) from a long-term field experiment (LTE) in Bernburg (Germany)managed either under cultivator tillage (CT) or moldboard plow (MP) in combinationwith either intensive nitrogen (N)-fertilization and pesticides (Int) or extensive reducedN-fertilization without fungicides (Ext), respectively. High-throughput sequencing of 16SrRNA gene and fungal ITS2 amplicons showed that changes in the microbial communitycomposition were correlated to differences in soil chemical properties caused by tillagepractice. Microbial communities of soils sampled in winter differed only depending onthe tillage practice while, in summer, also a strong effect of the fertilization intensity wasobserved. A small proportion of microbial taxa was shared between soils from the twosampling times, suggesting the existence of a stable core microbiota at the LTE. Ingeneral, taxa associated with organic matter decomposition (such as Actinobacteria,Bacteroidetes, Rhizopus, and Exophiala) had a higher relative abundance under CT.Among the taxa with significant changes in relative abundances due to different long-termagricultural practices were putative pathogenic (e.g., Gibellulopsis and Gibberella) andbeneficial microbial genera (e.g., Chitinophagaceae, Ferruginibacter, and Minimedusa).In summary, this study suggests that the effects of long-term agricultural managementpractices on the soil microbiota are influenced by the soil sampling time, and this needsto be kept in mind in future studies for the interpretation of field data.
publishDate 2022
dc.date.none.fl_str_mv 2022-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/203104
Fernandez Gnecco, Gabriela Amancay; Covacevich, Fernanda; Consolo, Verónica Fabiana; Behr, Jan H.; Sommermann, Loreen; et al.; Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling; Frontiers; Frontiers in Soil Science; 2; 3-2022; 1-16
2673-8619
CONICET Digital
CONICET
url http://hdl.handle.net/11336/203104
identifier_str_mv Fernandez Gnecco, Gabriela Amancay; Covacevich, Fernanda; Consolo, Verónica Fabiana; Behr, Jan H.; Sommermann, Loreen; et al.; Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling; Frontiers; Frontiers in Soil Science; 2; 3-2022; 1-16
2673-8619
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fsoil.2022.837508/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fsoil.2022.837508
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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