TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi

Autores
Sternlieb, Tamara; Schoijet, Alejandra Cecilia; Genta, Patricio D.; Alonso, Guillermo Daniel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme involved inmaintaining energy homeostasis in response to different stresses. During the transitionbetween the mammalian host and the insect vector, Trypanosoma cruzi (Chagasdisease), faces different environmental fluctuations, all of which prompt the parasite toremodel its metabolism to adapt and survive. Recently, it was shown that Trypanosomabrucei AMPK is involved in the differentiation process and in surface protein expressionchanges in response to nutritional stress.We identified four genes for the AMPK subunits of T. cruzi (α1 and α2 catalytic subunits,β and γ regulatory subunits). The β and γ subunits are largely conserved in their domainstructure. However, the alpha subunits show significant sequence, structure andevolutionary differences from the human counterparts. The presence of these subunits inT. cruzi epimastigotes was confirmed by several experimental approaches. TcAMPKαsubunits over-expression in epimastigotes showed opposite effects on growth rate. AMPKactivity is also upregulated in epimastigote under starvation. Each of these subunitscould complement the ?glucose dependent? phenotype of S. cerevisiae conditionalmutants lacking SNF1. Starvation assays with AMPKα over-expressing parasites alsoshowed a role of AMPK in autophagy. Our results confirm the presence of a functionalAMPK orthologue in T. cruzi. We also explore the pathways in which TcAMPK may beinvolved using in silico analysis of putative substrates.
Fil: Sternlieb, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Schoijet, Alejandra Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Genta, Patricio D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Parasitravaganza 2021
Smithfield
Australia
The Australian Society for Parasitology
Materia
CHAGAS DISEASE
AUTOPHAGY
ENERGY METHABOLISM
CARBON SOURCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/203416
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruziSternlieb, TamaraSchoijet, Alejandra CeciliaGenta, Patricio D.Alonso, Guillermo DanielCHAGAS DISEASEAUTOPHAGYENERGY METHABOLISMCARBON SOURCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme involved inmaintaining energy homeostasis in response to different stresses. During the transitionbetween the mammalian host and the insect vector, Trypanosoma cruzi (Chagasdisease), faces different environmental fluctuations, all of which prompt the parasite toremodel its metabolism to adapt and survive. Recently, it was shown that Trypanosomabrucei AMPK is involved in the differentiation process and in surface protein expressionchanges in response to nutritional stress.We identified four genes for the AMPK subunits of T. cruzi (α1 and α2 catalytic subunits,β and γ regulatory subunits). The β and γ subunits are largely conserved in their domainstructure. However, the alpha subunits show significant sequence, structure andevolutionary differences from the human counterparts. The presence of these subunits inT. cruzi epimastigotes was confirmed by several experimental approaches. TcAMPKαsubunits over-expression in epimastigotes showed opposite effects on growth rate. AMPKactivity is also upregulated in epimastigote under starvation. Each of these subunitscould complement the ?glucose dependent? phenotype of S. cerevisiae conditionalmutants lacking SNF1. Starvation assays with AMPKα over-expressing parasites alsoshowed a role of AMPK in autophagy. Our results confirm the presence of a functionalAMPK orthologue in T. cruzi. We also explore the pathways in which TcAMPK may beinvolved using in silico analysis of putative substrates.Fil: Sternlieb, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Schoijet, Alejandra Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Genta, Patricio D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaParasitravaganza 2021SmithfieldAustraliaThe Australian Society for ParasitologyThe Australian Society for Parasitology2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/203416TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi; Parasitravaganza 2021; Smithfield; Australia; 2020; 34-34CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.parasite.org.au/wp-content/uploads/2020/07/Parasitravaganza-Program-Booklet-4.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:38Zoai:ri.conicet.gov.ar:11336/203416instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:38.483CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi
title TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi
spellingShingle TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi
Sternlieb, Tamara
CHAGAS DISEASE
AUTOPHAGY
ENERGY METHABOLISM
CARBON SOURCE
title_short TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi
title_full TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi
title_fullStr TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi
title_full_unstemmed TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi
title_sort TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi
dc.creator.none.fl_str_mv Sternlieb, Tamara
Schoijet, Alejandra Cecilia
Genta, Patricio D.
Alonso, Guillermo Daniel
author Sternlieb, Tamara
author_facet Sternlieb, Tamara
Schoijet, Alejandra Cecilia
Genta, Patricio D.
Alonso, Guillermo Daniel
author_role author
author2 Schoijet, Alejandra Cecilia
Genta, Patricio D.
Alonso, Guillermo Daniel
author2_role author
author
author
dc.subject.none.fl_str_mv CHAGAS DISEASE
AUTOPHAGY
ENERGY METHABOLISM
CARBON SOURCE
topic CHAGAS DISEASE
AUTOPHAGY
ENERGY METHABOLISM
CARBON SOURCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme involved inmaintaining energy homeostasis in response to different stresses. During the transitionbetween the mammalian host and the insect vector, Trypanosoma cruzi (Chagasdisease), faces different environmental fluctuations, all of which prompt the parasite toremodel its metabolism to adapt and survive. Recently, it was shown that Trypanosomabrucei AMPK is involved in the differentiation process and in surface protein expressionchanges in response to nutritional stress.We identified four genes for the AMPK subunits of T. cruzi (α1 and α2 catalytic subunits,β and γ regulatory subunits). The β and γ subunits are largely conserved in their domainstructure. However, the alpha subunits show significant sequence, structure andevolutionary differences from the human counterparts. The presence of these subunits inT. cruzi epimastigotes was confirmed by several experimental approaches. TcAMPKαsubunits over-expression in epimastigotes showed opposite effects on growth rate. AMPKactivity is also upregulated in epimastigote under starvation. Each of these subunitscould complement the ?glucose dependent? phenotype of S. cerevisiae conditionalmutants lacking SNF1. Starvation assays with AMPKα over-expressing parasites alsoshowed a role of AMPK in autophagy. Our results confirm the presence of a functionalAMPK orthologue in T. cruzi. We also explore the pathways in which TcAMPK may beinvolved using in silico analysis of putative substrates.
Fil: Sternlieb, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Schoijet, Alejandra Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Genta, Patricio D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Parasitravaganza 2021
Smithfield
Australia
The Australian Society for Parasitology
description The AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme involved inmaintaining energy homeostasis in response to different stresses. During the transitionbetween the mammalian host and the insect vector, Trypanosoma cruzi (Chagasdisease), faces different environmental fluctuations, all of which prompt the parasite toremodel its metabolism to adapt and survive. Recently, it was shown that Trypanosomabrucei AMPK is involved in the differentiation process and in surface protein expressionchanges in response to nutritional stress.We identified four genes for the AMPK subunits of T. cruzi (α1 and α2 catalytic subunits,β and γ regulatory subunits). The β and γ subunits are largely conserved in their domainstructure. However, the alpha subunits show significant sequence, structure andevolutionary differences from the human counterparts. The presence of these subunits inT. cruzi epimastigotes was confirmed by several experimental approaches. TcAMPKαsubunits over-expression in epimastigotes showed opposite effects on growth rate. AMPKactivity is also upregulated in epimastigote under starvation. Each of these subunitscould complement the ?glucose dependent? phenotype of S. cerevisiae conditionalmutants lacking SNF1. Starvation assays with AMPKα over-expressing parasites alsoshowed a role of AMPK in autophagy. Our results confirm the presence of a functionalAMPK orthologue in T. cruzi. We also explore the pathways in which TcAMPK may beinvolved using in silico analysis of putative substrates.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/203416
TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi; Parasitravaganza 2021; Smithfield; Australia; 2020; 34-34
CONICET Digital
CONICET
url http://hdl.handle.net/11336/203416
identifier_str_mv TcAMPK: A cellular energy homeostasis hub regulator with unique characteristics in Trypanosoma cruzi; Parasitravaganza 2021; Smithfield; Australia; 2020; 34-34
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.parasite.org.au/wp-content/uploads/2020/07/Parasitravaganza-Program-Booklet-4.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv The Australian Society for Parasitology
publisher.none.fl_str_mv The Australian Society for Parasitology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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