Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice

Autores
Colombo, Lucas Luis; Vanzulli, Silvia I.; Blázquez Castro, Alfonso; Terrero, Clara Sanchez; Stockert, Juan C.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The photothermal effect is undergoing great interest due to advances in new photosensitizing materials and better-suited light sources, but studies are frequently hampered by the need to employ exogenous photothermal agents and expensive irradiation devices. Here we present a simple strategy based on direct NIR irradiation of the melanin pigment with a commercial 808-nm laser pointer. Proof-of-concept studies showed efficient photothermal effects on melanin in vitro and in vivo. After NIR irradiation, BALB/c mice bearing B16-F10 melanotic melanoma tumors revealed severe histopathological damage and massive necrosis in melanin-containing tumor tissue, while surrounding healthy tissues showed no damage. Therefore, the feasibility of this approach may allow implementing direct procedures for photothermal therapy of pigmented tumors.
Fil: Colombo, Lucas Luis. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Vanzulli, Silvia I.. Comisión Nacional de Energía Atómica; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Blázquez Castro, Alfonso. Universidad Nacional de Educacion A Distancia. Facultad de Ciencias.; España
Fil: Terrero, Clara Sanchez. Comisión Nacional de Energía Atómica; Argentina
Fil: Stockert, Juan C.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Materia
PHOTOTHERMAL EFFECT
LASER IRRADIATION
MELANOMA B16 F10
BALB/C MICE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/129341
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c miceColombo, Lucas LuisVanzulli, Silvia I.Blázquez Castro, AlfonsoTerrero, Clara SanchezStockert, Juan C.PHOTOTHERMAL EFFECTLASER IRRADIATIONMELANOMA B16 F10BALB/C MICEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The photothermal effect is undergoing great interest due to advances in new photosensitizing materials and better-suited light sources, but studies are frequently hampered by the need to employ exogenous photothermal agents and expensive irradiation devices. Here we present a simple strategy based on direct NIR irradiation of the melanin pigment with a commercial 808-nm laser pointer. Proof-of-concept studies showed efficient photothermal effects on melanin in vitro and in vivo. After NIR irradiation, BALB/c mice bearing B16-F10 melanotic melanoma tumors revealed severe histopathological damage and massive necrosis in melanin-containing tumor tissue, while surrounding healthy tissues showed no damage. Therefore, the feasibility of this approach may allow implementing direct procedures for photothermal therapy of pigmented tumors.Fil: Colombo, Lucas Luis. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Vanzulli, Silvia I.. Comisión Nacional de Energía Atómica; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Blázquez Castro, Alfonso. Universidad Nacional de Educacion A Distancia. Facultad de Ciencias.; EspañaFil: Terrero, Clara Sanchez. Comisión Nacional de Energía Atómica; ArgentinaFil: Stockert, Juan C.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaOptical Society of America2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/129341Colombo, Lucas Luis; Vanzulli, Silvia I.; Blázquez Castro, Alfonso; Terrero, Clara Sanchez; Stockert, Juan C.; Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice; Optical Society of America; Biomedical Optics Express; 10; 6; 6-2019; 2932-29412156-7085CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/abstract.cfm?URI=boe-10-6-2932info:eu-repo/semantics/altIdentifier/doi/10.1364/BOE.10.002932info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:50:11Zoai:ri.conicet.gov.ar:11336/129341instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:50:11.545CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice
title Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice
spellingShingle Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice
Colombo, Lucas Luis
PHOTOTHERMAL EFFECT
LASER IRRADIATION
MELANOMA B16 F10
BALB/C MICE
title_short Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice
title_full Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice
title_fullStr Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice
title_full_unstemmed Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice
title_sort Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice
dc.creator.none.fl_str_mv Colombo, Lucas Luis
Vanzulli, Silvia I.
Blázquez Castro, Alfonso
Terrero, Clara Sanchez
Stockert, Juan C.
author Colombo, Lucas Luis
author_facet Colombo, Lucas Luis
Vanzulli, Silvia I.
Blázquez Castro, Alfonso
Terrero, Clara Sanchez
Stockert, Juan C.
author_role author
author2 Vanzulli, Silvia I.
Blázquez Castro, Alfonso
Terrero, Clara Sanchez
Stockert, Juan C.
author2_role author
author
author
author
dc.subject.none.fl_str_mv PHOTOTHERMAL EFFECT
LASER IRRADIATION
MELANOMA B16 F10
BALB/C MICE
topic PHOTOTHERMAL EFFECT
LASER IRRADIATION
MELANOMA B16 F10
BALB/C MICE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The photothermal effect is undergoing great interest due to advances in new photosensitizing materials and better-suited light sources, but studies are frequently hampered by the need to employ exogenous photothermal agents and expensive irradiation devices. Here we present a simple strategy based on direct NIR irradiation of the melanin pigment with a commercial 808-nm laser pointer. Proof-of-concept studies showed efficient photothermal effects on melanin in vitro and in vivo. After NIR irradiation, BALB/c mice bearing B16-F10 melanotic melanoma tumors revealed severe histopathological damage and massive necrosis in melanin-containing tumor tissue, while surrounding healthy tissues showed no damage. Therefore, the feasibility of this approach may allow implementing direct procedures for photothermal therapy of pigmented tumors.
Fil: Colombo, Lucas Luis. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Vanzulli, Silvia I.. Comisión Nacional de Energía Atómica; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Blázquez Castro, Alfonso. Universidad Nacional de Educacion A Distancia. Facultad de Ciencias.; España
Fil: Terrero, Clara Sanchez. Comisión Nacional de Energía Atómica; Argentina
Fil: Stockert, Juan C.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
description The photothermal effect is undergoing great interest due to advances in new photosensitizing materials and better-suited light sources, but studies are frequently hampered by the need to employ exogenous photothermal agents and expensive irradiation devices. Here we present a simple strategy based on direct NIR irradiation of the melanin pigment with a commercial 808-nm laser pointer. Proof-of-concept studies showed efficient photothermal effects on melanin in vitro and in vivo. After NIR irradiation, BALB/c mice bearing B16-F10 melanotic melanoma tumors revealed severe histopathological damage and massive necrosis in melanin-containing tumor tissue, while surrounding healthy tissues showed no damage. Therefore, the feasibility of this approach may allow implementing direct procedures for photothermal therapy of pigmented tumors.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/129341
Colombo, Lucas Luis; Vanzulli, Silvia I.; Blázquez Castro, Alfonso; Terrero, Clara Sanchez; Stockert, Juan C.; Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice; Optical Society of America; Biomedical Optics Express; 10; 6; 6-2019; 2932-2941
2156-7085
CONICET Digital
CONICET
url http://hdl.handle.net/11336/129341
identifier_str_mv Colombo, Lucas Luis; Vanzulli, Silvia I.; Blázquez Castro, Alfonso; Terrero, Clara Sanchez; Stockert, Juan C.; Photothermal effect by 808-nm laser irradiation of melanin: A proof-of-concept study of photothermal therapy using b16-f10 melanotic melanoma growing in BALB/c mice; Optical Society of America; Biomedical Optics Express; 10; 6; 6-2019; 2932-2941
2156-7085
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/abstract.cfm?URI=boe-10-6-2932
info:eu-repo/semantics/altIdentifier/doi/10.1364/BOE.10.002932
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Optical Society of America
publisher.none.fl_str_mv Optical Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848598103167336448
score 13.3211565

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