QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry

Autores
Fontana, Ariel Ramón; Bottini, Ambrosio Rubén
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new method for determining 3-isopropyl-2-methoxypyrazine, 3-sec-butyl-2-methoxypyrazine, and 3-isobutyl-2-methoxypyrazine in wines is presented. A modified quick, easy, cheap, effective, rugged, and safe method and gas chromatography-mass spectrometry (GC-MS) were used for sample preparation and compound determination, respectively. The analytes were extracted from wine (30 mL) with 1 mL toluene, in the presence of 12 g anhydrous MgSO4 and 3 g NaCl. Cleanup of the toluene phase was performed by a miniaturized dispersive solid-phase extraction with a combination of anhydrous CaCl2 (25 mg), anhydrous MgSO4 (25 mg), and primary-secondary amine (10 mg), which was effective for minimizing co-extractives and matrix effects. GC-MS parameters were also tuned up to optimize limits of detection between 4.2 and 7.1 ng L−1. The overall recoveries (trueness) of the method ranged between 71 and 87 % for the white and red wine samples, respectively, spiked at 40 and 100 ng L−1, with relative standard deviations below 21 %. The method was applied for the determination of target methoxypyrazines in the samples of commercial wines from Argentina.
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
Dispersive Solid-Phase Extraction
Gas Chromatography-Mass Spectrometry
Methoxypyrazines
Quechers
Sample Preparation
Wine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39899
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/39899
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass SpectrometryFontana, Ariel RamónBottini, Ambrosio RubénDispersive Solid-Phase ExtractionGas Chromatography-Mass SpectrometryMethoxypyrazinesQuechersSample PreparationWinehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new method for determining 3-isopropyl-2-methoxypyrazine, 3-sec-butyl-2-methoxypyrazine, and 3-isobutyl-2-methoxypyrazine in wines is presented. A modified quick, easy, cheap, effective, rugged, and safe method and gas chromatography-mass spectrometry (GC-MS) were used for sample preparation and compound determination, respectively. The analytes were extracted from wine (30 mL) with 1 mL toluene, in the presence of 12 g anhydrous MgSO4 and 3 g NaCl. Cleanup of the toluene phase was performed by a miniaturized dispersive solid-phase extraction with a combination of anhydrous CaCl2 (25 mg), anhydrous MgSO4 (25 mg), and primary-secondary amine (10 mg), which was effective for minimizing co-extractives and matrix effects. GC-MS parameters were also tuned up to optimize limits of detection between 4.2 and 7.1 ng L−1. The overall recoveries (trueness) of the method ranged between 71 and 87 % for the white and red wine samples, respectively, spiked at 40 and 100 ng L−1, with relative standard deviations below 21 %. The method was applied for the determination of target methoxypyrazines in the samples of commercial wines from Argentina.Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaSpringer2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39899Fontana, Ariel Ramón; Bottini, Ambrosio Rubén; QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry; Springer; Food Analytical Methods; 9; 12; 12-2016; 3352-33591936-9751CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12161-016-0532-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12161-016-0532-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:38Zoai:ri.conicet.gov.ar:11336/39899instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:38.647CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry
title QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry
spellingShingle QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry
Fontana, Ariel Ramón
Dispersive Solid-Phase Extraction
Gas Chromatography-Mass Spectrometry
Methoxypyrazines
Quechers
Sample Preparation
Wine
title_short QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry
title_full QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry
title_fullStr QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry
title_full_unstemmed QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry
title_sort QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry
dc.creator.none.fl_str_mv Fontana, Ariel Ramón
Bottini, Ambrosio Rubén
author Fontana, Ariel Ramón
author_facet Fontana, Ariel Ramón
Bottini, Ambrosio Rubén
author_role author
author2 Bottini, Ambrosio Rubén
author2_role author
dc.subject.none.fl_str_mv Dispersive Solid-Phase Extraction
Gas Chromatography-Mass Spectrometry
Methoxypyrazines
Quechers
Sample Preparation
Wine
topic Dispersive Solid-Phase Extraction
Gas Chromatography-Mass Spectrometry
Methoxypyrazines
Quechers
Sample Preparation
Wine
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new method for determining 3-isopropyl-2-methoxypyrazine, 3-sec-butyl-2-methoxypyrazine, and 3-isobutyl-2-methoxypyrazine in wines is presented. A modified quick, easy, cheap, effective, rugged, and safe method and gas chromatography-mass spectrometry (GC-MS) were used for sample preparation and compound determination, respectively. The analytes were extracted from wine (30 mL) with 1 mL toluene, in the presence of 12 g anhydrous MgSO4 and 3 g NaCl. Cleanup of the toluene phase was performed by a miniaturized dispersive solid-phase extraction with a combination of anhydrous CaCl2 (25 mg), anhydrous MgSO4 (25 mg), and primary-secondary amine (10 mg), which was effective for minimizing co-extractives and matrix effects. GC-MS parameters were also tuned up to optimize limits of detection between 4.2 and 7.1 ng L−1. The overall recoveries (trueness) of the method ranged between 71 and 87 % for the white and red wine samples, respectively, spiked at 40 and 100 ng L−1, with relative standard deviations below 21 %. The method was applied for the determination of target methoxypyrazines in the samples of commercial wines from Argentina.
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description A new method for determining 3-isopropyl-2-methoxypyrazine, 3-sec-butyl-2-methoxypyrazine, and 3-isobutyl-2-methoxypyrazine in wines is presented. A modified quick, easy, cheap, effective, rugged, and safe method and gas chromatography-mass spectrometry (GC-MS) were used for sample preparation and compound determination, respectively. The analytes were extracted from wine (30 mL) with 1 mL toluene, in the presence of 12 g anhydrous MgSO4 and 3 g NaCl. Cleanup of the toluene phase was performed by a miniaturized dispersive solid-phase extraction with a combination of anhydrous CaCl2 (25 mg), anhydrous MgSO4 (25 mg), and primary-secondary amine (10 mg), which was effective for minimizing co-extractives and matrix effects. GC-MS parameters were also tuned up to optimize limits of detection between 4.2 and 7.1 ng L−1. The overall recoveries (trueness) of the method ranged between 71 and 87 % for the white and red wine samples, respectively, spiked at 40 and 100 ng L−1, with relative standard deviations below 21 %. The method was applied for the determination of target methoxypyrazines in the samples of commercial wines from Argentina.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39899
Fontana, Ariel Ramón; Bottini, Ambrosio Rubén; QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry; Springer; Food Analytical Methods; 9; 12; 12-2016; 3352-3359
1936-9751
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39899
identifier_str_mv Fontana, Ariel Ramón; Bottini, Ambrosio Rubén; QuEChERS Method for the Determination of 3‑Alkyl‑2‑Methoxypyrazines in Wines by Gas Chromatography-Mass Spectrometry; Springer; Food Analytical Methods; 9; 12; 12-2016; 3352-3359
1936-9751
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s12161-016-0532-4
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12161-016-0532-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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