Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology

Autores
Galeano, Pablo; Dalmasso, María Carolina; Prestia, Federico Ariel; Castaño, Eduardo Miguel; Cuello, Augusto Claudio; Santín Nuñez, Luis Javier; Morelli, Laura
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The contribution of adult hippocampal neurogenesis (AHN) impairment on cognitive decline in early Alzheimer disease (AD) remains poorly understood. This can be ascribed to the technical difficulties to measure AHN in postmortem brains and patients. Furthermore, most animal models of AD exhibit an aggressive neuropathology at early age and harbor gene mutations and express transgenes that disrupts AHN by pathways not directly involved in AD pathology. To overcome some of these limitations, we studied AHN at preplaque stage (6 month old) in hemizygous (Tg+/-) and homozygous (Tg+/+) McGill-R-Thy1-APP transgenic rats. This model exhibits a much less aggressive neuropathology that nevertheless is associated with a marked cognitive impairment from early age. Our results revealed that Tg+/+ rats showed a reduced number of PCNA+ cells, DCX+ immature neurons and BrdU+/NeuN+ colabed neurons in dorsal and ventral dentate gyrus. Moreover, dendritic arborization was less developed. AHN was not impaired in Tg+/- rats, although dendritic arborization was slightly decreased. On the other hand, both hemizygous and homozygous rats exhibited spatial memory impairments in the Morris water maze. These results suggest that 1) AHN is dysregulated from the preplaque stage in homozygous rats, 2) AHN impairment is dependent on APP transgene copy numbers since hemizygous rats did not show it, 3) Dysregulation of AHN is not directly associated with spatial memory impairments since hemizygous rats exhibited spared neurogenesis despite showing spatial memory deficits. Funding: International Society for Neurochemistry CAEN Grant and Andalucia TECH-ICE (PG), and PICT-2015-0285 (LM).
Fil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Dalmasso, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Prestia, Federico Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Castaño, Eduardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Cuello, Augusto Claudio. McGill University; Canadá
Fil: Santín Nuñez, Luis Javier. Universidad de Málaga; España
Fil: Morelli, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
The International Society for Neurochemistry and the American Society for Neurochemistry Meeting
Montreal
Canadá
International Society for Neurochemistry
American Society for Neurochemistry
Materia
ALZHEIMER
NEUROGENESIS
HIPPOCAMPUS
COGNITION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/152201
Acceder
id CONICETDig_7c639d6106221729a250b2199fa04a29
oai_identifier_str oai:ri.conicet.gov.ar:11336/152201
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathologyGaleano, PabloDalmasso, María CarolinaPrestia, Federico ArielCastaño, Eduardo MiguelCuello, Augusto ClaudioSantín Nuñez, Luis JavierMorelli, LauraALZHEIMERNEUROGENESISHIPPOCAMPUSCOGNITIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The contribution of adult hippocampal neurogenesis (AHN) impairment on cognitive decline in early Alzheimer disease (AD) remains poorly understood. This can be ascribed to the technical difficulties to measure AHN in postmortem brains and patients. Furthermore, most animal models of AD exhibit an aggressive neuropathology at early age and harbor gene mutations and express transgenes that disrupts AHN by pathways not directly involved in AD pathology. To overcome some of these limitations, we studied AHN at preplaque stage (6 month old) in hemizygous (Tg+/-) and homozygous (Tg+/+) McGill-R-Thy1-APP transgenic rats. This model exhibits a much less aggressive neuropathology that nevertheless is associated with a marked cognitive impairment from early age. Our results revealed that Tg+/+ rats showed a reduced number of PCNA+ cells, DCX+ immature neurons and BrdU+/NeuN+ colabed neurons in dorsal and ventral dentate gyrus. Moreover, dendritic arborization was less developed. AHN was not impaired in Tg+/- rats, although dendritic arborization was slightly decreased. On the other hand, both hemizygous and homozygous rats exhibited spatial memory impairments in the Morris water maze. These results suggest that 1) AHN is dysregulated from the preplaque stage in homozygous rats, 2) AHN impairment is dependent on APP transgene copy numbers since hemizygous rats did not show it, 3) Dysregulation of AHN is not directly associated with spatial memory impairments since hemizygous rats exhibited spared neurogenesis despite showing spatial memory deficits. Funding: International Society for Neurochemistry CAEN Grant and Andalucia TECH-ICE (PG), and PICT-2015-0285 (LM).Fil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Dalmasso, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Prestia, Federico Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Castaño, Eduardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Cuello, Augusto Claudio. McGill University; CanadáFil: Santín Nuñez, Luis Javier. Universidad de Málaga; EspañaFil: Morelli, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaThe International Society for Neurochemistry and the American Society for Neurochemistry MeetingMontrealCanadáInternational Society for NeurochemistryAmerican Society for NeurochemistryWiley Blackwell Publishing, Inc2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152201Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology; The International Society for Neurochemistry and the American Society for Neurochemistry Meeting; Montreal; Canadá; 2019; 1-21471-4159CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/jnc.14776Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:34Zoai:ri.conicet.gov.ar:11336/152201instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:34.855CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology
title Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology
spellingShingle Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology
Galeano, Pablo
ALZHEIMER
NEUROGENESIS
HIPPOCAMPUS
COGNITION
title_short Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology
title_full Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology
title_fullStr Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology
title_full_unstemmed Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology
title_sort Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology
dc.creator.none.fl_str_mv Galeano, Pablo
Dalmasso, María Carolina
Prestia, Federico Ariel
Castaño, Eduardo Miguel
Cuello, Augusto Claudio
Santín Nuñez, Luis Javier
Morelli, Laura
author Galeano, Pablo
author_facet Galeano, Pablo
Dalmasso, María Carolina
Prestia, Federico Ariel
Castaño, Eduardo Miguel
Cuello, Augusto Claudio
Santín Nuñez, Luis Javier
Morelli, Laura
author_role author
author2 Dalmasso, María Carolina
Prestia, Federico Ariel
Castaño, Eduardo Miguel
Cuello, Augusto Claudio
Santín Nuñez, Luis Javier
Morelli, Laura
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ALZHEIMER
NEUROGENESIS
HIPPOCAMPUS
COGNITION
topic ALZHEIMER
NEUROGENESIS
HIPPOCAMPUS
COGNITION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The contribution of adult hippocampal neurogenesis (AHN) impairment on cognitive decline in early Alzheimer disease (AD) remains poorly understood. This can be ascribed to the technical difficulties to measure AHN in postmortem brains and patients. Furthermore, most animal models of AD exhibit an aggressive neuropathology at early age and harbor gene mutations and express transgenes that disrupts AHN by pathways not directly involved in AD pathology. To overcome some of these limitations, we studied AHN at preplaque stage (6 month old) in hemizygous (Tg+/-) and homozygous (Tg+/+) McGill-R-Thy1-APP transgenic rats. This model exhibits a much less aggressive neuropathology that nevertheless is associated with a marked cognitive impairment from early age. Our results revealed that Tg+/+ rats showed a reduced number of PCNA+ cells, DCX+ immature neurons and BrdU+/NeuN+ colabed neurons in dorsal and ventral dentate gyrus. Moreover, dendritic arborization was less developed. AHN was not impaired in Tg+/- rats, although dendritic arborization was slightly decreased. On the other hand, both hemizygous and homozygous rats exhibited spatial memory impairments in the Morris water maze. These results suggest that 1) AHN is dysregulated from the preplaque stage in homozygous rats, 2) AHN impairment is dependent on APP transgene copy numbers since hemizygous rats did not show it, 3) Dysregulation of AHN is not directly associated with spatial memory impairments since hemizygous rats exhibited spared neurogenesis despite showing spatial memory deficits. Funding: International Society for Neurochemistry CAEN Grant and Andalucia TECH-ICE (PG), and PICT-2015-0285 (LM).
Fil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Dalmasso, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Prestia, Federico Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Castaño, Eduardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Cuello, Augusto Claudio. McGill University; Canadá
Fil: Santín Nuñez, Luis Javier. Universidad de Málaga; España
Fil: Morelli, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
The International Society for Neurochemistry and the American Society for Neurochemistry Meeting
Montreal
Canadá
International Society for Neurochemistry
American Society for Neurochemistry
description The contribution of adult hippocampal neurogenesis (AHN) impairment on cognitive decline in early Alzheimer disease (AD) remains poorly understood. This can be ascribed to the technical difficulties to measure AHN in postmortem brains and patients. Furthermore, most animal models of AD exhibit an aggressive neuropathology at early age and harbor gene mutations and express transgenes that disrupts AHN by pathways not directly involved in AD pathology. To overcome some of these limitations, we studied AHN at preplaque stage (6 month old) in hemizygous (Tg+/-) and homozygous (Tg+/+) McGill-R-Thy1-APP transgenic rats. This model exhibits a much less aggressive neuropathology that nevertheless is associated with a marked cognitive impairment from early age. Our results revealed that Tg+/+ rats showed a reduced number of PCNA+ cells, DCX+ immature neurons and BrdU+/NeuN+ colabed neurons in dorsal and ventral dentate gyrus. Moreover, dendritic arborization was less developed. AHN was not impaired in Tg+/- rats, although dendritic arborization was slightly decreased. On the other hand, both hemizygous and homozygous rats exhibited spatial memory impairments in the Morris water maze. These results suggest that 1) AHN is dysregulated from the preplaque stage in homozygous rats, 2) AHN impairment is dependent on APP transgene copy numbers since hemizygous rats did not show it, 3) Dysregulation of AHN is not directly associated with spatial memory impairments since hemizygous rats exhibited spared neurogenesis despite showing spatial memory deficits. Funding: International Society for Neurochemistry CAEN Grant and Andalucia TECH-ICE (PG), and PICT-2015-0285 (LM).
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/152201
Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology; The International Society for Neurochemistry and the American Society for Neurochemistry Meeting; Montreal; Canadá; 2019; 1-2
1471-4159
CONICET Digital
CONICET
url http://hdl.handle.net/11336/152201
identifier_str_mv Adult hippocampal neurogenesis impairment at preplaque stage in a transgenic rat model of Alzheimer like amyloid pathology; The International Society for Neurochemistry and the American Society for Neurochemistry Meeting; Montreal; Canadá; 2019; 1-2
1471-4159
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/jnc.14776
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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