Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis

Autores
Asselborn, Viviana Magdalena; Fernandez, Carolina; Zalocar, Yolanda; Parodi, Elisa Rosalia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of the organophosphorus insecticide chlorpyrifos on the growth, biovolume, and ultrastructure of the green microalga Ankistrodesmus gracilis was evaluated. Concentrations of 9.37, 18.75, 37.5, 75 and 150 mg L 1 of chlorpyrifos were assayed along with a control culture. At the end of the bioassay the ultrastructure of algal cells from control culture and from cultures exposed to 37.5 and 150 mg L 1 was observed under transmission (TEM) and scanning electron microscopy (SEM). After 24 and 48 h, treatments with 75 and 150 mg L 1 inhibited the growth of A. gracilis; whereas after 72 and 96 h, all the treatments except at 9.37 mg L 1 significantly affected the algae growth. The effective concentration 50 (EC50) after 96 h was 22.44 mg L 1 of chlorpyrifos. After the exposure to the insecticide, an increase in the biovolume was observed, with a larger increase in cells exposed to 75 and 150 mg L 1 . Radical changes were observed in the ultrastructure of cells exposed to chlorpyrifos. The insecticide affected the cell shape and the distribution of the crests in the wall. At 37.5 mg L 1 electodense bodies were observed along with an increase in the size and number of starch granules. At 150 mg L 1 such bodies occupied almost the whole cytoplasm together with lipids and remains of thylakoids. Autospores formation occurred normally at 37.5 mg L 1 while at 150 mg L 1 karyokinesis occurred, but cell-separation-phase was inhibited. The present study demonstrates that the exposure of phytoplankton to the insecticide chlorpyrifos leads to effects observed at both cellular and population level.
Fil: Asselborn, Viviana Magdalena. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ecología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina
Fil: Zalocar, Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentina
Fil: Parodi, Elisa Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina
Materia
Pesticide
Microalgae
Toxicity
Ultrastructure
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25193
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25193
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilisAsselborn, Viviana MagdalenaFernandez, CarolinaZalocar, YolandaParodi, Elisa RosaliaPesticideMicroalgaeToxicityUltrastructurehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effect of the organophosphorus insecticide chlorpyrifos on the growth, biovolume, and ultrastructure of the green microalga Ankistrodesmus gracilis was evaluated. Concentrations of 9.37, 18.75, 37.5, 75 and 150 mg L 1 of chlorpyrifos were assayed along with a control culture. At the end of the bioassay the ultrastructure of algal cells from control culture and from cultures exposed to 37.5 and 150 mg L 1 was observed under transmission (TEM) and scanning electron microscopy (SEM). After 24 and 48 h, treatments with 75 and 150 mg L 1 inhibited the growth of A. gracilis; whereas after 72 and 96 h, all the treatments except at 9.37 mg L 1 significantly affected the algae growth. The effective concentration 50 (EC50) after 96 h was 22.44 mg L 1 of chlorpyrifos. After the exposure to the insecticide, an increase in the biovolume was observed, with a larger increase in cells exposed to 75 and 150 mg L 1 . Radical changes were observed in the ultrastructure of cells exposed to chlorpyrifos. The insecticide affected the cell shape and the distribution of the crests in the wall. At 37.5 mg L 1 electodense bodies were observed along with an increase in the size and number of starch granules. At 150 mg L 1 such bodies occupied almost the whole cytoplasm together with lipids and remains of thylakoids. Autospores formation occurred normally at 37.5 mg L 1 while at 150 mg L 1 karyokinesis occurred, but cell-separation-phase was inhibited. The present study demonstrates that the exposure of phytoplankton to the insecticide chlorpyrifos leads to effects observed at both cellular and population level.Fil: Asselborn, Viviana Magdalena. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ecología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; ArgentinaFil: Zalocar, Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; ArgentinaFil: Parodi, Elisa Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; ArgentinaElsevier2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25193Asselborn, Viviana Magdalena; Fernandez, Carolina; Zalocar, Yolanda; Parodi, Elisa Rosalia; Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis; Elsevier; Ecotoxicology and Environmental Safety; 120; 10-2015; 334-3410147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2015.06.015info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0147651315003000info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:16:27Zoai:ri.conicet.gov.ar:11336/25193instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:16:27.811CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis
title Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis
spellingShingle Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis
Asselborn, Viviana Magdalena
Pesticide
Microalgae
Toxicity
Ultrastructure
title_short Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis
title_full Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis
title_fullStr Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis
title_full_unstemmed Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis
title_sort Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis
dc.creator.none.fl_str_mv Asselborn, Viviana Magdalena
Fernandez, Carolina
Zalocar, Yolanda
Parodi, Elisa Rosalia
author Asselborn, Viviana Magdalena
author_facet Asselborn, Viviana Magdalena
Fernandez, Carolina
Zalocar, Yolanda
Parodi, Elisa Rosalia
author_role author
author2 Fernandez, Carolina
Zalocar, Yolanda
Parodi, Elisa Rosalia
author2_role author
author
author
dc.subject.none.fl_str_mv Pesticide
Microalgae
Toxicity
Ultrastructure
topic Pesticide
Microalgae
Toxicity
Ultrastructure
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effect of the organophosphorus insecticide chlorpyrifos on the growth, biovolume, and ultrastructure of the green microalga Ankistrodesmus gracilis was evaluated. Concentrations of 9.37, 18.75, 37.5, 75 and 150 mg L 1 of chlorpyrifos were assayed along with a control culture. At the end of the bioassay the ultrastructure of algal cells from control culture and from cultures exposed to 37.5 and 150 mg L 1 was observed under transmission (TEM) and scanning electron microscopy (SEM). After 24 and 48 h, treatments with 75 and 150 mg L 1 inhibited the growth of A. gracilis; whereas after 72 and 96 h, all the treatments except at 9.37 mg L 1 significantly affected the algae growth. The effective concentration 50 (EC50) after 96 h was 22.44 mg L 1 of chlorpyrifos. After the exposure to the insecticide, an increase in the biovolume was observed, with a larger increase in cells exposed to 75 and 150 mg L 1 . Radical changes were observed in the ultrastructure of cells exposed to chlorpyrifos. The insecticide affected the cell shape and the distribution of the crests in the wall. At 37.5 mg L 1 electodense bodies were observed along with an increase in the size and number of starch granules. At 150 mg L 1 such bodies occupied almost the whole cytoplasm together with lipids and remains of thylakoids. Autospores formation occurred normally at 37.5 mg L 1 while at 150 mg L 1 karyokinesis occurred, but cell-separation-phase was inhibited. The present study demonstrates that the exposure of phytoplankton to the insecticide chlorpyrifos leads to effects observed at both cellular and population level.
Fil: Asselborn, Viviana Magdalena. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ecología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina
Fil: Zalocar, Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentina
Fil: Parodi, Elisa Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina
description The effect of the organophosphorus insecticide chlorpyrifos on the growth, biovolume, and ultrastructure of the green microalga Ankistrodesmus gracilis was evaluated. Concentrations of 9.37, 18.75, 37.5, 75 and 150 mg L 1 of chlorpyrifos were assayed along with a control culture. At the end of the bioassay the ultrastructure of algal cells from control culture and from cultures exposed to 37.5 and 150 mg L 1 was observed under transmission (TEM) and scanning electron microscopy (SEM). After 24 and 48 h, treatments with 75 and 150 mg L 1 inhibited the growth of A. gracilis; whereas after 72 and 96 h, all the treatments except at 9.37 mg L 1 significantly affected the algae growth. The effective concentration 50 (EC50) after 96 h was 22.44 mg L 1 of chlorpyrifos. After the exposure to the insecticide, an increase in the biovolume was observed, with a larger increase in cells exposed to 75 and 150 mg L 1 . Radical changes were observed in the ultrastructure of cells exposed to chlorpyrifos. The insecticide affected the cell shape and the distribution of the crests in the wall. At 37.5 mg L 1 electodense bodies were observed along with an increase in the size and number of starch granules. At 150 mg L 1 such bodies occupied almost the whole cytoplasm together with lipids and remains of thylakoids. Autospores formation occurred normally at 37.5 mg L 1 while at 150 mg L 1 karyokinesis occurred, but cell-separation-phase was inhibited. The present study demonstrates that the exposure of phytoplankton to the insecticide chlorpyrifos leads to effects observed at both cellular and population level.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25193
Asselborn, Viviana Magdalena; Fernandez, Carolina; Zalocar, Yolanda; Parodi, Elisa Rosalia; Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis; Elsevier; Ecotoxicology and Environmental Safety; 120; 10-2015; 334-341
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25193
identifier_str_mv Asselborn, Viviana Magdalena; Fernandez, Carolina; Zalocar, Yolanda; Parodi, Elisa Rosalia; Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis; Elsevier; Ecotoxicology and Environmental Safety; 120; 10-2015; 334-341
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2015.06.015
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0147651315003000
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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