Multiplex PCR for the detection and identification of dairy bacteriophages in milk

Autores
del Rio, B.; Binetti, Ana Griselda; Martín, M. C.; Fernández, M.; Magadán, A. H.; Alvarez, M. A.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacteriophage infections of starter lactic acid bacteria are a serious risk in the dairy industry. Phage infection can lead to slow lactic acid production or even the total failure of fermentation. The associated economic losses can be substantial. Rapid and sensitive methods are therefore required to detect and identify phages at all stages of the manufacture of fermented dairy products. This study describes a simple and rapid multiplex PCR method that, in a single reaction, detects the presence of bacteriophages infecting Streptococcus thermophilus and Lactobacillus delbrueckii, plus three genetically distinct ‘species’ of Lactococcus lactis phages commonly found in dairy plants (P335, 936 and c2). Available bacteriophage genome sequences were examined and the conserved regions used to design five pairs of primers, one for each of the above bacteriophage species. These primers were designed to generate specific fragments of different size depending on the species. Since this method can detect the above phages in untreated milk and can be easily incorporated into dairy industry routines, it might be readily used to earmark contaminated milk for use in processes that do not involve susceptible starter organisms or for use in those that involve phage-deactivating conditions.
Fil: del Rio, B.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; España
Fil: Binetti, Ana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Martín, M. C.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; España
Fil: Fernández, M.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; España
Fil: Magadán, A. H.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; España
Fil: Alvarez, M. A.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; España
Materia
DAIRY BACTERIOPHAGES
DETECTION
IDENTIFICATION
LACTOBACILLUS DELBRUECKII
LACTOCOCCUS LACTIS
MULTIPLEX-PCR
STREPTOCOCCUS THERMOPHILUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113996
Acceder
id CONICETDig_7bc4525b08d8e3c98dd424f94379be0a
oai_identifier_str oai:ri.conicet.gov.ar:11336/113996
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multiplex PCR for the detection and identification of dairy bacteriophages in milkdel Rio, B.Binetti, Ana GriseldaMartín, M. C.Fernández, M.Magadán, A. H.Alvarez, M. A.DAIRY BACTERIOPHAGESDETECTIONIDENTIFICATIONLACTOBACILLUS DELBRUECKIILACTOCOCCUS LACTISMULTIPLEX-PCRSTREPTOCOCCUS THERMOPHILUShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Bacteriophage infections of starter lactic acid bacteria are a serious risk in the dairy industry. Phage infection can lead to slow lactic acid production or even the total failure of fermentation. The associated economic losses can be substantial. Rapid and sensitive methods are therefore required to detect and identify phages at all stages of the manufacture of fermented dairy products. This study describes a simple and rapid multiplex PCR method that, in a single reaction, detects the presence of bacteriophages infecting Streptococcus thermophilus and Lactobacillus delbrueckii, plus three genetically distinct ‘species’ of Lactococcus lactis phages commonly found in dairy plants (P335, 936 and c2). Available bacteriophage genome sequences were examined and the conserved regions used to design five pairs of primers, one for each of the above bacteriophage species. These primers were designed to generate specific fragments of different size depending on the species. Since this method can detect the above phages in untreated milk and can be easily incorporated into dairy industry routines, it might be readily used to earmark contaminated milk for use in processes that do not involve susceptible starter organisms or for use in those that involve phage-deactivating conditions.Fil: del Rio, B.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; EspañaFil: Binetti, Ana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Martín, M. C.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; EspañaFil: Fernández, M.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; EspañaFil: Magadán, A. H.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; EspañaFil: Alvarez, M. A.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; EspañaAcademic Press Ltd - Elsevier Science Ltd2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113996del Rio, B.; Binetti, Ana Griselda; Martín, M. C.; Fernández, M.; Magadán, A. H.; et al.; Multiplex PCR for the detection and identification of dairy bacteriophages in milk; Academic Press Ltd - Elsevier Science Ltd; Food Microbiology; 24; 1; 6-2007; 75-810740-0020CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fm.2006.03.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:32:05Zoai:ri.conicet.gov.ar:11336/113996instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:32:06.219CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multiplex PCR for the detection and identification of dairy bacteriophages in milk
title Multiplex PCR for the detection and identification of dairy bacteriophages in milk
spellingShingle Multiplex PCR for the detection and identification of dairy bacteriophages in milk
del Rio, B.
DAIRY BACTERIOPHAGES
DETECTION
IDENTIFICATION
LACTOBACILLUS DELBRUECKII
LACTOCOCCUS LACTIS
MULTIPLEX-PCR
STREPTOCOCCUS THERMOPHILUS
title_short Multiplex PCR for the detection and identification of dairy bacteriophages in milk
title_full Multiplex PCR for the detection and identification of dairy bacteriophages in milk
title_fullStr Multiplex PCR for the detection and identification of dairy bacteriophages in milk
title_full_unstemmed Multiplex PCR for the detection and identification of dairy bacteriophages in milk
title_sort Multiplex PCR for the detection and identification of dairy bacteriophages in milk
dc.creator.none.fl_str_mv del Rio, B.
Binetti, Ana Griselda
Martín, M. C.
Fernández, M.
Magadán, A. H.
Alvarez, M. A.
author del Rio, B.
author_facet del Rio, B.
Binetti, Ana Griselda
Martín, M. C.
Fernández, M.
Magadán, A. H.
Alvarez, M. A.
author_role author
author2 Binetti, Ana Griselda
Martín, M. C.
Fernández, M.
Magadán, A. H.
Alvarez, M. A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DAIRY BACTERIOPHAGES
DETECTION
IDENTIFICATION
LACTOBACILLUS DELBRUECKII
LACTOCOCCUS LACTIS
MULTIPLEX-PCR
STREPTOCOCCUS THERMOPHILUS
topic DAIRY BACTERIOPHAGES
DETECTION
IDENTIFICATION
LACTOBACILLUS DELBRUECKII
LACTOCOCCUS LACTIS
MULTIPLEX-PCR
STREPTOCOCCUS THERMOPHILUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Bacteriophage infections of starter lactic acid bacteria are a serious risk in the dairy industry. Phage infection can lead to slow lactic acid production or even the total failure of fermentation. The associated economic losses can be substantial. Rapid and sensitive methods are therefore required to detect and identify phages at all stages of the manufacture of fermented dairy products. This study describes a simple and rapid multiplex PCR method that, in a single reaction, detects the presence of bacteriophages infecting Streptococcus thermophilus and Lactobacillus delbrueckii, plus three genetically distinct ‘species’ of Lactococcus lactis phages commonly found in dairy plants (P335, 936 and c2). Available bacteriophage genome sequences were examined and the conserved regions used to design five pairs of primers, one for each of the above bacteriophage species. These primers were designed to generate specific fragments of different size depending on the species. Since this method can detect the above phages in untreated milk and can be easily incorporated into dairy industry routines, it might be readily used to earmark contaminated milk for use in processes that do not involve susceptible starter organisms or for use in those that involve phage-deactivating conditions.
Fil: del Rio, B.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; España
Fil: Binetti, Ana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Martín, M. C.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; España
Fil: Fernández, M.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; España
Fil: Magadán, A. H.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; España
Fil: Alvarez, M. A.. Consejo Superior de Investigaciones Científicas. Instituto Superior de Productos Lácteos de Asturias; España
description Bacteriophage infections of starter lactic acid bacteria are a serious risk in the dairy industry. Phage infection can lead to slow lactic acid production or even the total failure of fermentation. The associated economic losses can be substantial. Rapid and sensitive methods are therefore required to detect and identify phages at all stages of the manufacture of fermented dairy products. This study describes a simple and rapid multiplex PCR method that, in a single reaction, detects the presence of bacteriophages infecting Streptococcus thermophilus and Lactobacillus delbrueckii, plus three genetically distinct ‘species’ of Lactococcus lactis phages commonly found in dairy plants (P335, 936 and c2). Available bacteriophage genome sequences were examined and the conserved regions used to design five pairs of primers, one for each of the above bacteriophage species. These primers were designed to generate specific fragments of different size depending on the species. Since this method can detect the above phages in untreated milk and can be easily incorporated into dairy industry routines, it might be readily used to earmark contaminated milk for use in processes that do not involve susceptible starter organisms or for use in those that involve phage-deactivating conditions.
publishDate 2007
dc.date.none.fl_str_mv 2007-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113996
del Rio, B.; Binetti, Ana Griselda; Martín, M. C.; Fernández, M.; Magadán, A. H.; et al.; Multiplex PCR for the detection and identification of dairy bacteriophages in milk; Academic Press Ltd - Elsevier Science Ltd; Food Microbiology; 24; 1; 6-2007; 75-81
0740-0020
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113996
identifier_str_mv del Rio, B.; Binetti, Ana Griselda; Martín, M. C.; Fernández, M.; Magadán, A. H.; et al.; Multiplex PCR for the detection and identification of dairy bacteriophages in milk; Academic Press Ltd - Elsevier Science Ltd; Food Microbiology; 24; 1; 6-2007; 75-81
0740-0020
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fm.2006.03.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082808135548928
score 13.221938

Publicaciones similares