Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Recepto...

Autores
Wenz, Jorge Javier; Borroni, Maria Virginia; Barrantes, Francisco Jose
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Extensive evidence supports the notion that the cytoskeleton participates in the immobilization and mem- brane clustering of the nicotinic acetylcholine receptor (AChR) at the neuromuscular junction. Stimulated emission depletion fluorescence microscopy has revealed the supra- molecular organization of AChR nanoclusters at the surface of CHO-K1/A5 cells with subdiffraction resolution (Kellner et al., Neuroscience 144:135–143 2007 ). We studied the effect of two cytoskeletal-disrupting drugs (cytochalasin D and jasplakinolide) on the nanoscale distribution of muscle- type AChR expressed in these cells by means of mathe- matical and statistical analysis of images obtained with the same high-resolution microscopy. AChR nanoclusters were found to be randomly distributed in both controls and cells treated with either drug for distances larger than 500 nm. Treatments altered the distribution of AChR nanoclusters according to their brightness/size. Cytochalasin D and jas- plakinolide produced a statistically significant increase in the proportion of medium-size nanoclusters and a diminution of small nanoclusters, indicating higher disrupting activity on the latter. This was further corroborated by the diminution of the brightness/diameter ratio of nanoclusters (a measure of the intracluster density of AChR molecules) and by Ripley’s analysis applied to simulated patterns with intracluster aggregation of AChR molecules. The combined analytical tools bring out subtle changes in the two-dimensional.
Fil: Wenz, Jorge Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Fil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Materia
Clustering
Stastical Analysis
Ripley´S Test
Poison
Achr
Sted
Cytoskeleton
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42456
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/42456
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine ReceptorWenz, Jorge JavierBorroni, Maria VirginiaBarrantes, Francisco JoseClusteringStastical AnalysisRipley´S TestPoisonAchrStedCytoskeletonhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Extensive evidence supports the notion that the cytoskeleton participates in the immobilization and mem- brane clustering of the nicotinic acetylcholine receptor (AChR) at the neuromuscular junction. Stimulated emission depletion fluorescence microscopy has revealed the supra- molecular organization of AChR nanoclusters at the surface of CHO-K1/A5 cells with subdiffraction resolution (Kellner et al., Neuroscience 144:135–143 2007 ). We studied the effect of two cytoskeletal-disrupting drugs (cytochalasin D and jasplakinolide) on the nanoscale distribution of muscle- type AChR expressed in these cells by means of mathe- matical and statistical analysis of images obtained with the same high-resolution microscopy. AChR nanoclusters were found to be randomly distributed in both controls and cells treated with either drug for distances larger than 500 nm. Treatments altered the distribution of AChR nanoclusters according to their brightness/size. Cytochalasin D and jas- plakinolide produced a statistically significant increase in the proportion of medium-size nanoclusters and a diminution of small nanoclusters, indicating higher disrupting activity on the latter. This was further corroborated by the diminution of the brightness/diameter ratio of nanoclusters (a measure of the intracluster density of AChR molecules) and by Ripley’s analysis applied to simulated patterns with intracluster aggregation of AChR molecules. The combined analytical tools bring out subtle changes in the two-dimensional.Fil: Wenz, Jorge Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; ArgentinaFil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; ArgentinaFil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; ArgentinaSpringer2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42456Wenz, Jorge Javier; Borroni, Maria Virginia; Barrantes, Francisco Jose; Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor; Springer; Journal of Membrane Biology; 235; 3; 6-2010; 163-1750022-2631CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.springerlink.com/content/8457p60198424148/info:eu-repo/semantics/altIdentifier/doi/10.1007/s00232-010-9261-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:54Zoai:ri.conicet.gov.ar:11336/42456instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:55.168CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor
title Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor
spellingShingle Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor
Wenz, Jorge Javier
Clustering
Stastical Analysis
Ripley´S Test
Poison
Achr
Sted
Cytoskeleton
title_short Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor
title_full Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor
title_fullStr Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor
title_full_unstemmed Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor
title_sort Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor
dc.creator.none.fl_str_mv Wenz, Jorge Javier
Borroni, Maria Virginia
Barrantes, Francisco Jose
author Wenz, Jorge Javier
author_facet Wenz, Jorge Javier
Borroni, Maria Virginia
Barrantes, Francisco Jose
author_role author
author2 Borroni, Maria Virginia
Barrantes, Francisco Jose
author2_role author
author
dc.subject.none.fl_str_mv Clustering
Stastical Analysis
Ripley´S Test
Poison
Achr
Sted
Cytoskeleton
topic Clustering
Stastical Analysis
Ripley´S Test
Poison
Achr
Sted
Cytoskeleton
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Extensive evidence supports the notion that the cytoskeleton participates in the immobilization and mem- brane clustering of the nicotinic acetylcholine receptor (AChR) at the neuromuscular junction. Stimulated emission depletion fluorescence microscopy has revealed the supra- molecular organization of AChR nanoclusters at the surface of CHO-K1/A5 cells with subdiffraction resolution (Kellner et al., Neuroscience 144:135–143 2007 ). We studied the effect of two cytoskeletal-disrupting drugs (cytochalasin D and jasplakinolide) on the nanoscale distribution of muscle- type AChR expressed in these cells by means of mathe- matical and statistical analysis of images obtained with the same high-resolution microscopy. AChR nanoclusters were found to be randomly distributed in both controls and cells treated with either drug for distances larger than 500 nm. Treatments altered the distribution of AChR nanoclusters according to their brightness/size. Cytochalasin D and jas- plakinolide produced a statistically significant increase in the proportion of medium-size nanoclusters and a diminution of small nanoclusters, indicating higher disrupting activity on the latter. This was further corroborated by the diminution of the brightness/diameter ratio of nanoclusters (a measure of the intracluster density of AChR molecules) and by Ripley’s analysis applied to simulated patterns with intracluster aggregation of AChR molecules. The combined analytical tools bring out subtle changes in the two-dimensional.
Fil: Wenz, Jorge Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Fil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
description Extensive evidence supports the notion that the cytoskeleton participates in the immobilization and mem- brane clustering of the nicotinic acetylcholine receptor (AChR) at the neuromuscular junction. Stimulated emission depletion fluorescence microscopy has revealed the supra- molecular organization of AChR nanoclusters at the surface of CHO-K1/A5 cells with subdiffraction resolution (Kellner et al., Neuroscience 144:135–143 2007 ). We studied the effect of two cytoskeletal-disrupting drugs (cytochalasin D and jasplakinolide) on the nanoscale distribution of muscle- type AChR expressed in these cells by means of mathe- matical and statistical analysis of images obtained with the same high-resolution microscopy. AChR nanoclusters were found to be randomly distributed in both controls and cells treated with either drug for distances larger than 500 nm. Treatments altered the distribution of AChR nanoclusters according to their brightness/size. Cytochalasin D and jas- plakinolide produced a statistically significant increase in the proportion of medium-size nanoclusters and a diminution of small nanoclusters, indicating higher disrupting activity on the latter. This was further corroborated by the diminution of the brightness/diameter ratio of nanoclusters (a measure of the intracluster density of AChR molecules) and by Ripley’s analysis applied to simulated patterns with intracluster aggregation of AChR molecules. The combined analytical tools bring out subtle changes in the two-dimensional.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42456
Wenz, Jorge Javier; Borroni, Maria Virginia; Barrantes, Francisco Jose; Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor; Springer; Journal of Membrane Biology; 235; 3; 6-2010; 163-175
0022-2631
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42456
identifier_str_mv Wenz, Jorge Javier; Borroni, Maria Virginia; Barrantes, Francisco Jose; Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor; Springer; Journal of Membrane Biology; 235; 3; 6-2010; 163-175
0022-2631
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.springerlink.com/content/8457p60198424148/
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00232-010-9261-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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