Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.

Autores
la Colla, Anabela Belén; Vasconsuelo, Andrea Anahi; Boland, Ricardo Leopoldo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
17beta-Estradiol (E2) protects several non-reproductive tissues from apoptosis, including skeletal muscle. We have shown that E2 at physiological concentrations prevented apoptosis induced by H2O2 in C2C12 skeletal myoblasts. As we also demonstrated the presence of estrogen receptors in mitochondria, the present work was focused on the effects of E2 on this organelle. Specifically, we evaluated the actions of E2 on the mitochondrial permeability transition pore (MPTP) by the calcein-acetoxymethylester/cobalt method using fluorescence microscopy and flow cytometry. Pretreatment with E2 prevented MPTP opening induced by H2O2, which preceded loss of mitochondrial membrane potential. In addition, it was observed that H2O2 induced translocation of Bax to mitochondria; however, in the presence of the steroid this effect was abrogated suggesting that members of the Bcl-2 family may be regulated by E2 to exert an antiapoptotic effect. Moreover, E2 increased mitochondrial manganese superoxide dismutase protein expression and activity, as part of a mechanism activated by E2 that improved mitochondrial performance. Our results suggest a role of E2 in the regulation of apoptosis with a clear action at the mitochondrial level in C2C12 skeletal myoblast cells.
Fil: la Colla, Anabela Belén. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vasconsuelo, Andrea Anahi. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Boland, Ricardo Leopoldo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
17 BETA ESTRADIOL
ANTIAPOPTOTIC
MPTP
MNSOD
C2C12 SKELETAL MYOBLASTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10118
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10118
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.la Colla, Anabela BelénVasconsuelo, Andrea AnahiBoland, Ricardo Leopoldo17 BETA ESTRADIOLANTIAPOPTOTICMPTPMNSODC2C12 SKELETAL MYOBLASTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/117beta-Estradiol (E2) protects several non-reproductive tissues from apoptosis, including skeletal muscle. We have shown that E2 at physiological concentrations prevented apoptosis induced by H2O2 in C2C12 skeletal myoblasts. As we also demonstrated the presence of estrogen receptors in mitochondria, the present work was focused on the effects of E2 on this organelle. Specifically, we evaluated the actions of E2 on the mitochondrial permeability transition pore (MPTP) by the calcein-acetoxymethylester/cobalt method using fluorescence microscopy and flow cytometry. Pretreatment with E2 prevented MPTP opening induced by H2O2, which preceded loss of mitochondrial membrane potential. In addition, it was observed that H2O2 induced translocation of Bax to mitochondria; however, in the presence of the steroid this effect was abrogated suggesting that members of the Bcl-2 family may be regulated by E2 to exert an antiapoptotic effect. Moreover, E2 increased mitochondrial manganese superoxide dismutase protein expression and activity, as part of a mechanism activated by E2 that improved mitochondrial performance. Our results suggest a role of E2 in the regulation of apoptosis with a clear action at the mitochondrial level in C2C12 skeletal myoblast cells.Fil: la Colla, Anabela Belén. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vasconsuelo, Andrea Anahi. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Boland, Ricardo Leopoldo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioScientifica2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10118la Colla, Anabela Belén; Vasconsuelo, Andrea Anahi; Boland, Ricardo Leopoldo; Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.; BioScientifica; Journal of Endocrinology; 216; 3; 3-2013; 331-3410022-0795enginfo:eu-repo/semantics/altIdentifier/url/http://joe.endocrinology-journals.org/content/216/3/331info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/23213199info:eu-repo/semantics/altIdentifier/doi/10.1530/JOE-12-0486info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:18Zoai:ri.conicet.gov.ar:11336/10118instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:18.552CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.
title Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.
spellingShingle Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.
la Colla, Anabela Belén
17 BETA ESTRADIOL
ANTIAPOPTOTIC
MPTP
MNSOD
C2C12 SKELETAL MYOBLASTS
title_short Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.
title_full Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.
title_fullStr Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.
title_full_unstemmed Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.
title_sort Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.
dc.creator.none.fl_str_mv la Colla, Anabela Belén
Vasconsuelo, Andrea Anahi
Boland, Ricardo Leopoldo
author la Colla, Anabela Belén
author_facet la Colla, Anabela Belén
Vasconsuelo, Andrea Anahi
Boland, Ricardo Leopoldo
author_role author
author2 Vasconsuelo, Andrea Anahi
Boland, Ricardo Leopoldo
author2_role author
author
dc.subject.none.fl_str_mv 17 BETA ESTRADIOL
ANTIAPOPTOTIC
MPTP
MNSOD
C2C12 SKELETAL MYOBLASTS
topic 17 BETA ESTRADIOL
ANTIAPOPTOTIC
MPTP
MNSOD
C2C12 SKELETAL MYOBLASTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv 17beta-Estradiol (E2) protects several non-reproductive tissues from apoptosis, including skeletal muscle. We have shown that E2 at physiological concentrations prevented apoptosis induced by H2O2 in C2C12 skeletal myoblasts. As we also demonstrated the presence of estrogen receptors in mitochondria, the present work was focused on the effects of E2 on this organelle. Specifically, we evaluated the actions of E2 on the mitochondrial permeability transition pore (MPTP) by the calcein-acetoxymethylester/cobalt method using fluorescence microscopy and flow cytometry. Pretreatment with E2 prevented MPTP opening induced by H2O2, which preceded loss of mitochondrial membrane potential. In addition, it was observed that H2O2 induced translocation of Bax to mitochondria; however, in the presence of the steroid this effect was abrogated suggesting that members of the Bcl-2 family may be regulated by E2 to exert an antiapoptotic effect. Moreover, E2 increased mitochondrial manganese superoxide dismutase protein expression and activity, as part of a mechanism activated by E2 that improved mitochondrial performance. Our results suggest a role of E2 in the regulation of apoptosis with a clear action at the mitochondrial level in C2C12 skeletal myoblast cells.
Fil: la Colla, Anabela Belén. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vasconsuelo, Andrea Anahi. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Boland, Ricardo Leopoldo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description 17beta-Estradiol (E2) protects several non-reproductive tissues from apoptosis, including skeletal muscle. We have shown that E2 at physiological concentrations prevented apoptosis induced by H2O2 in C2C12 skeletal myoblasts. As we also demonstrated the presence of estrogen receptors in mitochondria, the present work was focused on the effects of E2 on this organelle. Specifically, we evaluated the actions of E2 on the mitochondrial permeability transition pore (MPTP) by the calcein-acetoxymethylester/cobalt method using fluorescence microscopy and flow cytometry. Pretreatment with E2 prevented MPTP opening induced by H2O2, which preceded loss of mitochondrial membrane potential. In addition, it was observed that H2O2 induced translocation of Bax to mitochondria; however, in the presence of the steroid this effect was abrogated suggesting that members of the Bcl-2 family may be regulated by E2 to exert an antiapoptotic effect. Moreover, E2 increased mitochondrial manganese superoxide dismutase protein expression and activity, as part of a mechanism activated by E2 that improved mitochondrial performance. Our results suggest a role of E2 in the regulation of apoptosis with a clear action at the mitochondrial level in C2C12 skeletal myoblast cells.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10118
la Colla, Anabela Belén; Vasconsuelo, Andrea Anahi; Boland, Ricardo Leopoldo; Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.; BioScientifica; Journal of Endocrinology; 216; 3; 3-2013; 331-341
0022-0795
url http://hdl.handle.net/11336/10118
identifier_str_mv la Colla, Anabela Belén; Vasconsuelo, Andrea Anahi; Boland, Ricardo Leopoldo; Estradiol exerts antiapoptotic effects in skeletal myoblasts via mitochondrial PTP and MnSOD.; BioScientifica; Journal of Endocrinology; 216; 3; 3-2013; 331-341
0022-0795
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://joe.endocrinology-journals.org/content/216/3/331
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/23213199
info:eu-repo/semantics/altIdentifier/doi/10.1530/JOE-12-0486
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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