Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device

Autores
Figueroa, Rodolfo; Lozano, E.; Bongiovanni, Guillermina Azucena
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Visualization of elemental distributions of biological tissue is gaining importance in many disciplines of biological, forensic, and medical research. On the other hand, the mapping of elements has wider application to the archaeological, to understanding pigments, modes of preservation, and environmental context. Since major advances in relation to collimators and detectors have yielded micro scale images, the chemical mapping via synchrotron scanning micro-X-ray fluorescence spectrometry (SR-µXRF) is widely used as microanalytical techniques. However, the acquisition time is a limitation of current SR-µXRF imaging protocols, doing tedious micro analysis of samples of more than 1 cm and very difficult to study of larger samples such as animal organ, whole organisms, work of art, etc. Recently we have developed a robotic system to image the chemistry of large specimens rapidly at concentration levels of parts per million. Multiple images of distribution of elements can be obtained on surfaces of 100x100 mm and a spatial resolution of up to 0.2 mm2 per pixel, with a spectral capture time up to 1 ms per point. This system has proven to be highly efficient for the XRF mapping of elements in large biological samples, achieving comparables results to those obtained by SR-μXRF. Thus, images of As and Cu accumulation in renal cortex of arsenic-exposed rats were obtained by both methodologies. However, the new imaging system enables the XRF scanning in few minutes, whereas SR-μXRF required several hours. These and other advantages as well as the potential applications of this system, will be discussed.
Fil: Figueroa, Rodolfo. Universidad de la Frontera; Chile
Fil: Lozano, E.. Instituto Nacional del Cancer; Chile
Fil: Bongiovanni, Guillermina Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Patagonia Norte. Instituto de Investigación y Des. En Ing. de Procesos, Biotecnología y Energias Alternativas. Idepa - Subsede San Antonio Oeste; Argentina
Materia
XRF-Imaging
biological samples
multi-elemental mapping
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18598
Acceder
id CONICETDig_7a5171faf98ba40acc4e50cbd5e8b267
oai_identifier_str oai:ri.conicet.gov.ar:11336/18598
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Element distribution imaging in rat kidney using a 2D rapid scan EDXRF deviceFigueroa, RodolfoLozano, E.Bongiovanni, Guillermina AzucenaXRF-Imagingbiological samplesmulti-elemental mappinghttps://purl.org/becyt/ford/2.6https://purl.org/becyt/ford/2Visualization of elemental distributions of biological tissue is gaining importance in many disciplines of biological, forensic, and medical research. On the other hand, the mapping of elements has wider application to the archaeological, to understanding pigments, modes of preservation, and environmental context. Since major advances in relation to collimators and detectors have yielded micro scale images, the chemical mapping via synchrotron scanning micro-X-ray fluorescence spectrometry (SR-µXRF) is widely used as microanalytical techniques. However, the acquisition time is a limitation of current SR-µXRF imaging protocols, doing tedious micro analysis of samples of more than 1 cm and very difficult to study of larger samples such as animal organ, whole organisms, work of art, etc. Recently we have developed a robotic system to image the chemistry of large specimens rapidly at concentration levels of parts per million. Multiple images of distribution of elements can be obtained on surfaces of 100x100 mm and a spatial resolution of up to 0.2 mm2 per pixel, with a spectral capture time up to 1 ms per point. This system has proven to be highly efficient for the XRF mapping of elements in large biological samples, achieving comparables results to those obtained by SR-μXRF. Thus, images of As and Cu accumulation in renal cortex of arsenic-exposed rats were obtained by both methodologies. However, the new imaging system enables the XRF scanning in few minutes, whereas SR-μXRF required several hours. These and other advantages as well as the potential applications of this system, will be discussed.Fil: Figueroa, Rodolfo. Universidad de la Frontera; ChileFil: Lozano, E.. Instituto Nacional del Cancer; ChileFil: Bongiovanni, Guillermina Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Patagonia Norte. Instituto de Investigación y Des. En Ing. de Procesos, Biotecnología y Energias Alternativas. Idepa - Subsede San Antonio Oeste; ArgentinaSoc Mexicana Fisica2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18598Figueroa, Rodolfo; Lozano, E.; Bongiovanni, Guillermina Azucena; Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device; Soc Mexicana Fisica; Revista Mexicana de Física; 59; 6-2013; 262-2660035-001Xenginfo:eu-repo/semantics/altIdentifier/url/http://rmf.smf.mx/pdf/rmf/59/4/59_4_292.pdfinfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0035-001X2013000400002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:50Zoai:ri.conicet.gov.ar:11336/18598instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:51.184CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device
title Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device
spellingShingle Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device
Figueroa, Rodolfo
XRF-Imaging
biological samples
multi-elemental mapping
title_short Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device
title_full Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device
title_fullStr Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device
title_full_unstemmed Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device
title_sort Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device
dc.creator.none.fl_str_mv Figueroa, Rodolfo
Lozano, E.
Bongiovanni, Guillermina Azucena
author Figueroa, Rodolfo
author_facet Figueroa, Rodolfo
Lozano, E.
Bongiovanni, Guillermina Azucena
author_role author
author2 Lozano, E.
Bongiovanni, Guillermina Azucena
author2_role author
author
dc.subject.none.fl_str_mv XRF-Imaging
biological samples
multi-elemental mapping
topic XRF-Imaging
biological samples
multi-elemental mapping
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.6
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Visualization of elemental distributions of biological tissue is gaining importance in many disciplines of biological, forensic, and medical research. On the other hand, the mapping of elements has wider application to the archaeological, to understanding pigments, modes of preservation, and environmental context. Since major advances in relation to collimators and detectors have yielded micro scale images, the chemical mapping via synchrotron scanning micro-X-ray fluorescence spectrometry (SR-µXRF) is widely used as microanalytical techniques. However, the acquisition time is a limitation of current SR-µXRF imaging protocols, doing tedious micro analysis of samples of more than 1 cm and very difficult to study of larger samples such as animal organ, whole organisms, work of art, etc. Recently we have developed a robotic system to image the chemistry of large specimens rapidly at concentration levels of parts per million. Multiple images of distribution of elements can be obtained on surfaces of 100x100 mm and a spatial resolution of up to 0.2 mm2 per pixel, with a spectral capture time up to 1 ms per point. This system has proven to be highly efficient for the XRF mapping of elements in large biological samples, achieving comparables results to those obtained by SR-μXRF. Thus, images of As and Cu accumulation in renal cortex of arsenic-exposed rats were obtained by both methodologies. However, the new imaging system enables the XRF scanning in few minutes, whereas SR-μXRF required several hours. These and other advantages as well as the potential applications of this system, will be discussed.
Fil: Figueroa, Rodolfo. Universidad de la Frontera; Chile
Fil: Lozano, E.. Instituto Nacional del Cancer; Chile
Fil: Bongiovanni, Guillermina Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Patagonia Norte. Instituto de Investigación y Des. En Ing. de Procesos, Biotecnología y Energias Alternativas. Idepa - Subsede San Antonio Oeste; Argentina
description Visualization of elemental distributions of biological tissue is gaining importance in many disciplines of biological, forensic, and medical research. On the other hand, the mapping of elements has wider application to the archaeological, to understanding pigments, modes of preservation, and environmental context. Since major advances in relation to collimators and detectors have yielded micro scale images, the chemical mapping via synchrotron scanning micro-X-ray fluorescence spectrometry (SR-µXRF) is widely used as microanalytical techniques. However, the acquisition time is a limitation of current SR-µXRF imaging protocols, doing tedious micro analysis of samples of more than 1 cm and very difficult to study of larger samples such as animal organ, whole organisms, work of art, etc. Recently we have developed a robotic system to image the chemistry of large specimens rapidly at concentration levels of parts per million. Multiple images of distribution of elements can be obtained on surfaces of 100x100 mm and a spatial resolution of up to 0.2 mm2 per pixel, with a spectral capture time up to 1 ms per point. This system has proven to be highly efficient for the XRF mapping of elements in large biological samples, achieving comparables results to those obtained by SR-μXRF. Thus, images of As and Cu accumulation in renal cortex of arsenic-exposed rats were obtained by both methodologies. However, the new imaging system enables the XRF scanning in few minutes, whereas SR-μXRF required several hours. These and other advantages as well as the potential applications of this system, will be discussed.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18598
Figueroa, Rodolfo; Lozano, E.; Bongiovanni, Guillermina Azucena; Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device; Soc Mexicana Fisica; Revista Mexicana de Física; 59; 6-2013; 262-266
0035-001X
url http://hdl.handle.net/11336/18598
identifier_str_mv Figueroa, Rodolfo; Lozano, E.; Bongiovanni, Guillermina Azucena; Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device; Soc Mexicana Fisica; Revista Mexicana de Física; 59; 6-2013; 262-266
0035-001X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://rmf.smf.mx/pdf/rmf/59/4/59_4_292.pdf
info:eu-repo/semantics/altIdentifier/url/http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0035-001X2013000400002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Soc Mexicana Fisica
publisher.none.fl_str_mv Soc Mexicana Fisica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614135284760576
score 13.070432

Publicaciones similares