Different binding motifs of the celiac disease-associated HLA molecules DQ2.5, DQ2.2, and DQ7.5 revealed by relative quantitative proteomics of endogenous peptide repertoires

• Autores: Bergseng, Elin; Dørum, Siri; Arntzen, Magnus Ø.; Nielsen, Morten; Nygård, Ståle; Buus, Søren; de Souza, Gustavo A.; Sollid, Ludvig M.
• Año de publicación: 2014
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Antigen presentation/processing; Celiac disease; Binding motif; Mass spectrometry; MHC
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Celiac disease is caused by intolerance to cereal gluten proteins, and HLA-DQ molecules are involved in the disease pathogenesis by presentation of gluten peptides to CD4+ T cells. The α- or β-chain sharing HLA molecules DQ2.5, DQ2.2, and DQ7.5 display different risks for the disease. It was recently demonstrated that T cells of DQ2.5 and DQ2.2 patients recognize distinct sets of gluten epitopes, suggesting that these two DQ2 variants select different peptides for display. To explore whether this is the case, we performed a comprehensive comparison of the endogenous self-peptides bound to HLA-DQ molecules of B-lymphoblastoid cell lines. Peptides were eluted from affinity-purified HLA molecules of nine cell lines and subjected to quadrupole orbitrap mass spectrometry and MaxQuant software analysis. Altogether, 12,712 endogenous peptides were identified at very different relative abundances. Hierarchical clustering of normalized quantitative data demonstrated significant differences in repertoires of peptides between the three DQ variant molecules. The neural network-based method, NNAlign, was used to identify peptide-binding motifs. The binding motifs of DQ2.5 and DQ7.5 concurred with previously established binding motifs. The binding motif of DQ2.2 was strikingly different from that of DQ2.5 with position P3 being a major anchor having a preference for threonine and serine. This is notable as three recently identified epitopes of gluten recognized by T cells of DQ2.2 celiac patients harbor serine at position P3. This study demonstrates that relative quantitative comparison of endogenous peptides sampled from our protein metabolism by HLA molecules provides clues to understand HLA association with disease.
Fil: Bergseng, Elin. University of Oslo; Noruega
Fil: Dørum, Siri. University of Oslo; Noruega
Fil: Arntzen, Magnus Ø.. University of Oslo; Noruega
Fil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Nygård, Ståle. University of Oslo; Noruega
Fil: Buus, Søren. Universidad de Copenhagen; Dinamarca
Fil: de Souza, Gustavo A.. University of Oslo; Noruega
Fil: Sollid, Ludvig M.. University of Oslo; Noruega