Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload

Autores
Roson, Maria Ines; Della Penna, Silvana L.; Cao, Gabriel Fernando; Gorzalczany, Susana Beatriz; Pandolfo, Marcela; Toblli, Jorge Eduardo; Fernandez, Belisario Enrique
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to study the role of local intrarenal angiotensin II (Ang II) and the oxidative stress in the up-regulation of pro-inflammatory cytokines expression observed in rats submitted to an acute sodium overload. Sprague–Dawley rats were infused for 2 h with isotonic saline solution (Control group) and with hypertonic saline solution alone (Na group), plus the AT1 receptor antagonist losartan (10 mg kg 1 in bolus) (Na–Los group), or plus the superoxide dismutase mimetic tempol (0.5 mg min 1 kg 1 ) (Na–Temp group). Mean arterial pressure, glomerular filtration rate, and fractional sodium excretion (FENa) were measured. Ang II, NF-kB, hypoxia inducible factor-1a (HIF-1a), transforming growth factor b1 (TGF-b1), smooth muscle actin (a-SMA), endothelial nitric oxide synthase (eNOS), and RANTES renal expression was evaluated by immunohistochemistry. Ang II, NF-kB, and TGF-b1 and RANTES early inflammatory markers were overexpressed in Na group, accompanied by enhanced HIF-1a immunostaining, lower eNOS expression, and unmodified a-SMA. Losartan and tempol increased FENa in sodium overload group. Although losartan reduced Ang II and NF-kB staining and increased eNOS expression, it did not restore HIF-1a expression and did not prevent inflammation. Conversely, tempol increased eNOS and natriuresis, restored HIF-1a expression, and prevented inflammation. Early inflammatory markers observed in rats with acute sodium overload is associated with the imbalance between HIF-1a and eNOS expression. While both losartan and tempol increased natriuresis and eNOS expression, only tempol was effective in restoring HIF-1a expression and down-regulating TGF-b1 and RANTES expression. The protective role of tempol, but not of losartan, in the inflammatory response may be associated with its greater antioxidant effects.
Fil: Roson, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Della Penna, Silvana L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Cao, Gabriel Fernando. Hospital Alemán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gorzalczany, Susana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Pandolfo, Marcela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Toblli, Jorge Eduardo. Hospital Alemán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Belisario Enrique. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
LOSARTAN
TEMPOL
KIDNEY
SODIUM OVERLOAD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280320
Acceder
id CONICETDig_7967d2b744cf95d5de1b4578c3aacf94
oai_identifier_str oai:ri.conicet.gov.ar:11336/280320
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overloadRoson, Maria InesDella Penna, Silvana L.Cao, Gabriel FernandoGorzalczany, Susana BeatrizPandolfo, MarcelaToblli, Jorge EduardoFernandez, Belisario EnriqueLOSARTANTEMPOLKIDNEYSODIUM OVERLOADhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The aim of this work was to study the role of local intrarenal angiotensin II (Ang II) and the oxidative stress in the up-regulation of pro-inflammatory cytokines expression observed in rats submitted to an acute sodium overload. Sprague–Dawley rats were infused for 2 h with isotonic saline solution (Control group) and with hypertonic saline solution alone (Na group), plus the AT1 receptor antagonist losartan (10 mg kg 1 in bolus) (Na–Los group), or plus the superoxide dismutase mimetic tempol (0.5 mg min 1 kg 1 ) (Na–Temp group). Mean arterial pressure, glomerular filtration rate, and fractional sodium excretion (FENa) were measured. Ang II, NF-kB, hypoxia inducible factor-1a (HIF-1a), transforming growth factor b1 (TGF-b1), smooth muscle actin (a-SMA), endothelial nitric oxide synthase (eNOS), and RANTES renal expression was evaluated by immunohistochemistry. Ang II, NF-kB, and TGF-b1 and RANTES early inflammatory markers were overexpressed in Na group, accompanied by enhanced HIF-1a immunostaining, lower eNOS expression, and unmodified a-SMA. Losartan and tempol increased FENa in sodium overload group. Although losartan reduced Ang II and NF-kB staining and increased eNOS expression, it did not restore HIF-1a expression and did not prevent inflammation. Conversely, tempol increased eNOS and natriuresis, restored HIF-1a expression, and prevented inflammation. Early inflammatory markers observed in rats with acute sodium overload is associated with the imbalance between HIF-1a and eNOS expression. While both losartan and tempol increased natriuresis and eNOS expression, only tempol was effective in restoring HIF-1a expression and down-regulating TGF-b1 and RANTES expression. The protective role of tempol, but not of losartan, in the inflammatory response may be associated with its greater antioxidant effects.Fil: Roson, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Della Penna, Silvana L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Cao, Gabriel Fernando. Hospital Alemán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gorzalczany, Susana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Pandolfo, Marcela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Toblli, Jorge Eduardo. Hospital Alemán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Belisario Enrique. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley-liss, div John Wiley & Sons Inc.2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280320Roson, Maria Ines; Della Penna, Silvana L.; Cao, Gabriel Fernando; Gorzalczany, Susana Beatriz; Pandolfo, Marcela; et al.; Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload; Wiley-liss, div John Wiley & Sons Inc.; Journal of Cellular Physiology; 224; 1; 1-2010; 41-480021-9541CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.22087info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T09:58:12Zoai:ri.conicet.gov.ar:11336/280320instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 09:58:13.156CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload
title Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload
spellingShingle Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload
Roson, Maria Ines
LOSARTAN
TEMPOL
KIDNEY
SODIUM OVERLOAD
title_short Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload
title_full Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload
title_fullStr Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload
title_full_unstemmed Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload
title_sort Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload
dc.creator.none.fl_str_mv Roson, Maria Ines
Della Penna, Silvana L.
Cao, Gabriel Fernando
Gorzalczany, Susana Beatriz
Pandolfo, Marcela
Toblli, Jorge Eduardo
Fernandez, Belisario Enrique
author Roson, Maria Ines
author_facet Roson, Maria Ines
Della Penna, Silvana L.
Cao, Gabriel Fernando
Gorzalczany, Susana Beatriz
Pandolfo, Marcela
Toblli, Jorge Eduardo
Fernandez, Belisario Enrique
author_role author
author2 Della Penna, Silvana L.
Cao, Gabriel Fernando
Gorzalczany, Susana Beatriz
Pandolfo, Marcela
Toblli, Jorge Eduardo
Fernandez, Belisario Enrique
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv LOSARTAN
TEMPOL
KIDNEY
SODIUM OVERLOAD
topic LOSARTAN
TEMPOL
KIDNEY
SODIUM OVERLOAD
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this work was to study the role of local intrarenal angiotensin II (Ang II) and the oxidative stress in the up-regulation of pro-inflammatory cytokines expression observed in rats submitted to an acute sodium overload. Sprague–Dawley rats were infused for 2 h with isotonic saline solution (Control group) and with hypertonic saline solution alone (Na group), plus the AT1 receptor antagonist losartan (10 mg kg 1 in bolus) (Na–Los group), or plus the superoxide dismutase mimetic tempol (0.5 mg min 1 kg 1 ) (Na–Temp group). Mean arterial pressure, glomerular filtration rate, and fractional sodium excretion (FENa) were measured. Ang II, NF-kB, hypoxia inducible factor-1a (HIF-1a), transforming growth factor b1 (TGF-b1), smooth muscle actin (a-SMA), endothelial nitric oxide synthase (eNOS), and RANTES renal expression was evaluated by immunohistochemistry. Ang II, NF-kB, and TGF-b1 and RANTES early inflammatory markers were overexpressed in Na group, accompanied by enhanced HIF-1a immunostaining, lower eNOS expression, and unmodified a-SMA. Losartan and tempol increased FENa in sodium overload group. Although losartan reduced Ang II and NF-kB staining and increased eNOS expression, it did not restore HIF-1a expression and did not prevent inflammation. Conversely, tempol increased eNOS and natriuresis, restored HIF-1a expression, and prevented inflammation. Early inflammatory markers observed in rats with acute sodium overload is associated with the imbalance between HIF-1a and eNOS expression. While both losartan and tempol increased natriuresis and eNOS expression, only tempol was effective in restoring HIF-1a expression and down-regulating TGF-b1 and RANTES expression. The protective role of tempol, but not of losartan, in the inflammatory response may be associated with its greater antioxidant effects.
Fil: Roson, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Della Penna, Silvana L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Cao, Gabriel Fernando. Hospital Alemán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gorzalczany, Susana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Pandolfo, Marcela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Toblli, Jorge Eduardo. Hospital Alemán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Belisario Enrique. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of this work was to study the role of local intrarenal angiotensin II (Ang II) and the oxidative stress in the up-regulation of pro-inflammatory cytokines expression observed in rats submitted to an acute sodium overload. Sprague–Dawley rats were infused for 2 h with isotonic saline solution (Control group) and with hypertonic saline solution alone (Na group), plus the AT1 receptor antagonist losartan (10 mg kg 1 in bolus) (Na–Los group), or plus the superoxide dismutase mimetic tempol (0.5 mg min 1 kg 1 ) (Na–Temp group). Mean arterial pressure, glomerular filtration rate, and fractional sodium excretion (FENa) were measured. Ang II, NF-kB, hypoxia inducible factor-1a (HIF-1a), transforming growth factor b1 (TGF-b1), smooth muscle actin (a-SMA), endothelial nitric oxide synthase (eNOS), and RANTES renal expression was evaluated by immunohistochemistry. Ang II, NF-kB, and TGF-b1 and RANTES early inflammatory markers were overexpressed in Na group, accompanied by enhanced HIF-1a immunostaining, lower eNOS expression, and unmodified a-SMA. Losartan and tempol increased FENa in sodium overload group. Although losartan reduced Ang II and NF-kB staining and increased eNOS expression, it did not restore HIF-1a expression and did not prevent inflammation. Conversely, tempol increased eNOS and natriuresis, restored HIF-1a expression, and prevented inflammation. Early inflammatory markers observed in rats with acute sodium overload is associated with the imbalance between HIF-1a and eNOS expression. While both losartan and tempol increased natriuresis and eNOS expression, only tempol was effective in restoring HIF-1a expression and down-regulating TGF-b1 and RANTES expression. The protective role of tempol, but not of losartan, in the inflammatory response may be associated with its greater antioxidant effects.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280320
Roson, Maria Ines; Della Penna, Silvana L.; Cao, Gabriel Fernando; Gorzalczany, Susana Beatriz; Pandolfo, Marcela; et al.; Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload; Wiley-liss, div John Wiley & Sons Inc.; Journal of Cellular Physiology; 224; 1; 1-2010; 41-48
0021-9541
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280320
identifier_str_mv Roson, Maria Ines; Della Penna, Silvana L.; Cao, Gabriel Fernando; Gorzalczany, Susana Beatriz; Pandolfo, Marcela; et al.; Different protective actions of losartan and tempol on the renal inflammatory response to acute sodium overload; Wiley-liss, div John Wiley & Sons Inc.; Journal of Cellular Physiology; 224; 1; 1-2010; 41-48
0021-9541
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.22087
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176822

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