The translational landscape of the splicing factor SRSF1 and its role in mitosis

Autores
Maslon, Magdalena M.; Heras, Sara R.; Bellora, Nicolás; Eyras, Eduardo; Cáceres, Javier F.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The shuttling serine/arginine rich (SR) protein SRSF1 (previously known as SF2/ASF) is a splicing regulator that also activates translation in the cytoplasm. In order to dissect the gene network that is translationally regulated by SRSF1, we performed a high-throughput deep sequencing analysis of polysomal fractions in cells overexpressing SRSF1. We identified approximately 1500 mRNAs that are translational targets of SRSF1. These include mRNAs encoding proteins involved in cell cycle regulation, such as spindle, kinetochore, and M phase proteins, which are essential for accurate chromosome segregation. Indeed, we show that translational activity of SRSF1 is required for normal mitotic progression. Furthermore, we found that mRNAs that display alternative splicing changes upon SRSF1 overexpression are also its translational targets, strongly suggesting that SRSF1 couples pre-mRNA splicing and translation. These data provide insights on the complex role of SRSF1 in the control of gene expression at multiple levels and its implications in cancer.
Fil: Maslon, Magdalena M. . Institute of Genetics and Molecular Medicine, University of Edinburgh; Reino Unido
Fil: Heras, Sara R.. Institute of Genetics and Molecular Medicine, University of Edinburgh; Reino Unido. Universidad de Granada; España
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación En Biodiversidad y Medioambiente; Argentina. Universitat Pompeu Fabra; España
Fil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Catalan Institution for Research and Advanced Studies (ICREA), Barcelona; España
Fil: Cáceres, Javier F.. University Of Edinburgh; Reino Unido
Materia
Biochemistry
Cell biology
Bioinformatics
Human
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7225
Acceder
id CONICETDig_78af715c618a8901cbaf04034ff17f17
oai_identifier_str oai:ri.conicet.gov.ar:11336/7225
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The translational landscape of the splicing factor SRSF1 and its role in mitosisMaslon, Magdalena M. Heras, Sara R.Bellora, NicolásEyras, EduardoCáceres, Javier F.BiochemistryCell biologyBioinformaticsHumanhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The shuttling serine/arginine rich (SR) protein SRSF1 (previously known as SF2/ASF) is a splicing regulator that also activates translation in the cytoplasm. In order to dissect the gene network that is translationally regulated by SRSF1, we performed a high-throughput deep sequencing analysis of polysomal fractions in cells overexpressing SRSF1. We identified approximately 1500 mRNAs that are translational targets of SRSF1. These include mRNAs encoding proteins involved in cell cycle regulation, such as spindle, kinetochore, and M phase proteins, which are essential for accurate chromosome segregation. Indeed, we show that translational activity of SRSF1 is required for normal mitotic progression. Furthermore, we found that mRNAs that display alternative splicing changes upon SRSF1 overexpression are also its translational targets, strongly suggesting that SRSF1 couples pre-mRNA splicing and translation. These data provide insights on the complex role of SRSF1 in the control of gene expression at multiple levels and its implications in cancer.Fil: Maslon, Magdalena M. . Institute of Genetics and Molecular Medicine, University of Edinburgh; Reino UnidoFil: Heras, Sara R.. Institute of Genetics and Molecular Medicine, University of Edinburgh; Reino Unido. Universidad de Granada; EspañaFil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación En Biodiversidad y Medioambiente; Argentina. Universitat Pompeu Fabra; EspañaFil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Catalan Institution for Research and Advanced Studies (ICREA), Barcelona; EspañaFil: Cáceres, Javier F.. University Of Edinburgh; Reino UnidoeLife Sciences Publications2014-05-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7225Maslon, Magdalena M. ; Heras, Sara R.; Bellora, Nicolás; Eyras, Eduardo; Cáceres, Javier F.; The translational landscape of the splicing factor SRSF1 and its role in mitosis; eLife Sciences Publications; eLIFE; 3; 6-5-2014; e02028-e020282050-084Xenginfo:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.02028info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/content/3/e02028/article-metricsinfo:eu-repo/semantics/altIdentifier/pmid/24842991info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/content/3/e02028info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:26Zoai:ri.conicet.gov.ar:11336/7225instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:26.615CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The translational landscape of the splicing factor SRSF1 and its role in mitosis
title The translational landscape of the splicing factor SRSF1 and its role in mitosis
spellingShingle The translational landscape of the splicing factor SRSF1 and its role in mitosis
Maslon, Magdalena M.
Biochemistry
Cell biology
Bioinformatics
Human
title_short The translational landscape of the splicing factor SRSF1 and its role in mitosis
title_full The translational landscape of the splicing factor SRSF1 and its role in mitosis
title_fullStr The translational landscape of the splicing factor SRSF1 and its role in mitosis
title_full_unstemmed The translational landscape of the splicing factor SRSF1 and its role in mitosis
title_sort The translational landscape of the splicing factor SRSF1 and its role in mitosis
dc.creator.none.fl_str_mv Maslon, Magdalena M.
Heras, Sara R.
Bellora, Nicolás
Eyras, Eduardo
Cáceres, Javier F.
author Maslon, Magdalena M.
author_facet Maslon, Magdalena M.
Heras, Sara R.
Bellora, Nicolás
Eyras, Eduardo
Cáceres, Javier F.
author_role author
author2 Heras, Sara R.
Bellora, Nicolás
Eyras, Eduardo
Cáceres, Javier F.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biochemistry
Cell biology
Bioinformatics
Human
topic Biochemistry
Cell biology
Bioinformatics
Human
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The shuttling serine/arginine rich (SR) protein SRSF1 (previously known as SF2/ASF) is a splicing regulator that also activates translation in the cytoplasm. In order to dissect the gene network that is translationally regulated by SRSF1, we performed a high-throughput deep sequencing analysis of polysomal fractions in cells overexpressing SRSF1. We identified approximately 1500 mRNAs that are translational targets of SRSF1. These include mRNAs encoding proteins involved in cell cycle regulation, such as spindle, kinetochore, and M phase proteins, which are essential for accurate chromosome segregation. Indeed, we show that translational activity of SRSF1 is required for normal mitotic progression. Furthermore, we found that mRNAs that display alternative splicing changes upon SRSF1 overexpression are also its translational targets, strongly suggesting that SRSF1 couples pre-mRNA splicing and translation. These data provide insights on the complex role of SRSF1 in the control of gene expression at multiple levels and its implications in cancer.
Fil: Maslon, Magdalena M. . Institute of Genetics and Molecular Medicine, University of Edinburgh; Reino Unido
Fil: Heras, Sara R.. Institute of Genetics and Molecular Medicine, University of Edinburgh; Reino Unido. Universidad de Granada; España
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación En Biodiversidad y Medioambiente; Argentina. Universitat Pompeu Fabra; España
Fil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Catalan Institution for Research and Advanced Studies (ICREA), Barcelona; España
Fil: Cáceres, Javier F.. University Of Edinburgh; Reino Unido
description The shuttling serine/arginine rich (SR) protein SRSF1 (previously known as SF2/ASF) is a splicing regulator that also activates translation in the cytoplasm. In order to dissect the gene network that is translationally regulated by SRSF1, we performed a high-throughput deep sequencing analysis of polysomal fractions in cells overexpressing SRSF1. We identified approximately 1500 mRNAs that are translational targets of SRSF1. These include mRNAs encoding proteins involved in cell cycle regulation, such as spindle, kinetochore, and M phase proteins, which are essential for accurate chromosome segregation. Indeed, we show that translational activity of SRSF1 is required for normal mitotic progression. Furthermore, we found that mRNAs that display alternative splicing changes upon SRSF1 overexpression are also its translational targets, strongly suggesting that SRSF1 couples pre-mRNA splicing and translation. These data provide insights on the complex role of SRSF1 in the control of gene expression at multiple levels and its implications in cancer.
publishDate 2014
dc.date.none.fl_str_mv 2014-05-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7225
Maslon, Magdalena M. ; Heras, Sara R.; Bellora, Nicolás; Eyras, Eduardo; Cáceres, Javier F.; The translational landscape of the splicing factor SRSF1 and its role in mitosis; eLife Sciences Publications; eLIFE; 3; 6-5-2014; e02028-e02028
2050-084X
url http://hdl.handle.net/11336/7225
identifier_str_mv Maslon, Magdalena M. ; Heras, Sara R.; Bellora, Nicolás; Eyras, Eduardo; Cáceres, Javier F.; The translational landscape of the splicing factor SRSF1 and its role in mitosis; eLife Sciences Publications; eLIFE; 3; 6-5-2014; e02028-e02028
2050-084X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.02028
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/content/3/e02028/article-metrics
info:eu-repo/semantics/altIdentifier/pmid/24842991
info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/content/3/e02028
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268730975846400
score 13.13397

Publicaciones similares