Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa...

Autores: Laurito, Magdalena; Ayala, Ana Maria; Almiron, Walter Ricardo; Gardenal, Cristina Noemi
Año de publicación: 2022
Idioma: español castellano
Tipo de recurso: conjunto de datos
Estado
Descripción: Culex bidens and C. interfor, implicated in arbovirus transmission in Argentina, are sister species, only distinguishable by feature of the male genitalia; however, intermediate specimens of the species in sympatry have been found. Fourth-instar larvae and females of both species share apomorphic features, and this lack of clear distinction creates problems for specific identification. Geometric morphometric traits of these life stages also do not distinguish the species. The aim of the present study was to assess the taxonomic status of C. bidens and C. interfor using two mitochondrial genes and to determine the degree of their reproductive isolation using microsatellite loci. Sequences of the ND4 and COI genes were concatenated in a matrix of 993 nucleotides and used for phylogenetic and distance analyses. Bayesian and maximum parsimony inferences showed a well resolved and supported topology, enclosing sequences of individuals of C. bidens (0.83 BPP, 73 BSV) and C. interfor (0.98 BPP, 97 BSV) in a strong sister relationship. The mean K2P distance within C. bidens and C. interfor was 0.3% and 0.2%, respectively, and the interspecific variation was 2.3%. Bayesian clustering also showed two distinct mitochondrial lineages. All sequenced mosquitoes were successfully identified in accordance with the best close match algorithm. The low genetic distance values obtained indicate that the species diverged quite recently. Most morphologically intermediate specimens of C. bidens from Córdoba were heterozygous for the microsatellite locus GT51; the significant heterozygote excess observed suggests incomplete reproductive isolation. However, C. bidens and C. interfor should be considered good species: the ventral arm of the phallosome of the male genitalia and the ND4 and COI sequences are diagnostic characters.
Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Ayala, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Almiron, Walter Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Gardenal, Cristina Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/176475

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spelling	Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de ArgentinaLaurito, MagdalenaAyala, Ana MariaAlmiron, Walter RicardoGardenal, Cristina Noemihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Culex bidens and C. interfor, implicated in arbovirus transmission in Argentina, are sister species, only distinguishable by feature of the male genitalia; however, intermediate specimens of the species in sympatry have been found. Fourth-instar larvae and females of both species share apomorphic features, and this lack of clear distinction creates problems for specific identification. Geometric morphometric traits of these life stages also do not distinguish the species. The aim of the present study was to assess the taxonomic status of C. bidens and C. interfor using two mitochondrial genes and to determine the degree of their reproductive isolation using microsatellite loci. Sequences of the ND4 and COI genes were concatenated in a matrix of 993 nucleotides and used for phylogenetic and distance analyses. Bayesian and maximum parsimony inferences showed a well resolved and supported topology, enclosing sequences of individuals of C. bidens (0.83 BPP, 73 BSV) and C. interfor (0.98 BPP, 97 BSV) in a strong sister relationship. The mean K2P distance within C. bidens and C. interfor was 0.3% and 0.2%, respectively, and the interspecific variation was 2.3%. Bayesian clustering also showed two distinct mitochondrial lineages. All sequenced mosquitoes were successfully identified in accordance with the best close match algorithm. The low genetic distance values obtained indicate that the species diverged quite recently. Most morphologically intermediate specimens of C. bidens from Córdoba were heterozygous for the microsatellite locus GT51; the significant heterozygote excess observed suggests incomplete reproductive isolation. However, C. bidens and C. interfor should be considered good species: the ventral arm of the phallosome of the male genitalia and the ND4 and COI sequences are diagnostic characters.Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Ayala, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Almiron, Walter Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Gardenal, Cristina Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina2022info:ar-repo/semantics/conjuntoDeDatosv1.0info:eu-repo/semantics/dataSetapplication/octet-streamapplication/octet-streamhttp://hdl.handle.net/11336/176475Laurito, Magdalena; Ayala, Ana Maria; Almiron, Walter Ricardo; Gardenal, Cristina Noemi; (2022): Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/176475CONICET DigitalCONICETspainfo:eu-repo/grantAgreement/Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica/2013-1254info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:19:49Zoai:ri.conicet.gov.ar:11336/176475instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:19:50.272CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina
title	Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina
spellingShingle	Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina Laurito, Magdalena
title_short	Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina
title_full	Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina
title_fullStr	Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina
title_full_unstemmed	Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina
title_sort	Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina
dc.creator.none.fl_str_mv	Laurito, Magdalena Ayala, Ana Maria Almiron, Walter Ricardo Gardenal, Cristina Noemi
author	Laurito, Magdalena
author_facet	Laurito, Magdalena Ayala, Ana Maria Almiron, Walter Ricardo Gardenal, Cristina Noemi
author_role	author
author2	Ayala, Ana Maria Almiron, Walter Ricardo Gardenal, Cristina Noemi
author2_role	author author author
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Culex bidens and C. interfor, implicated in arbovirus transmission in Argentina, are sister species, only distinguishable by feature of the male genitalia; however, intermediate specimens of the species in sympatry have been found. Fourth-instar larvae and females of both species share apomorphic features, and this lack of clear distinction creates problems for specific identification. Geometric morphometric traits of these life stages also do not distinguish the species. The aim of the present study was to assess the taxonomic status of C. bidens and C. interfor using two mitochondrial genes and to determine the degree of their reproductive isolation using microsatellite loci. Sequences of the ND4 and COI genes were concatenated in a matrix of 993 nucleotides and used for phylogenetic and distance analyses. Bayesian and maximum parsimony inferences showed a well resolved and supported topology, enclosing sequences of individuals of C. bidens (0.83 BPP, 73 BSV) and C. interfor (0.98 BPP, 97 BSV) in a strong sister relationship. The mean K2P distance within C. bidens and C. interfor was 0.3% and 0.2%, respectively, and the interspecific variation was 2.3%. Bayesian clustering also showed two distinct mitochondrial lineages. All sequenced mosquitoes were successfully identified in accordance with the best close match algorithm. The low genetic distance values obtained indicate that the species diverged quite recently. Most morphologically intermediate specimens of C. bidens from Córdoba were heterozygous for the microsatellite locus GT51; the significant heterozygote excess observed suggests incomplete reproductive isolation. However, C. bidens and C. interfor should be considered good species: the ventral arm of the phallosome of the male genitalia and the ND4 and COI sequences are diagnostic characters. Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Ayala, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina Fil: Almiron, Walter Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Gardenal, Cristina Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
description	Culex bidens and C. interfor, implicated in arbovirus transmission in Argentina, are sister species, only distinguishable by feature of the male genitalia; however, intermediate specimens of the species in sympatry have been found. Fourth-instar larvae and females of both species share apomorphic features, and this lack of clear distinction creates problems for specific identification. Geometric morphometric traits of these life stages also do not distinguish the species. The aim of the present study was to assess the taxonomic status of C. bidens and C. interfor using two mitochondrial genes and to determine the degree of their reproductive isolation using microsatellite loci. Sequences of the ND4 and COI genes were concatenated in a matrix of 993 nucleotides and used for phylogenetic and distance analyses. Bayesian and maximum parsimony inferences showed a well resolved and supported topology, enclosing sequences of individuals of C. bidens (0.83 BPP, 73 BSV) and C. interfor (0.98 BPP, 97 BSV) in a strong sister relationship. The mean K2P distance within C. bidens and C. interfor was 0.3% and 0.2%, respectively, and the interspecific variation was 2.3%. Bayesian clustering also showed two distinct mitochondrial lineages. All sequenced mosquitoes were successfully identified in accordance with the best close match algorithm. The low genetic distance values obtained indicate that the species diverged quite recently. Most morphologically intermediate specimens of C. bidens from Córdoba were heterozygous for the microsatellite locus GT51; the significant heterozygote excess observed suggests incomplete reproductive isolation. However, C. bidens and C. interfor should be considered good species: the ventral arm of the phallosome of the male genitalia and the ND4 and COI sequences are diagnostic characters.
publishDate	2022
dc.date.none.fl_str_mv	2022
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dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/176475 Laurito, Magdalena; Ayala, Ana Maria; Almiron, Walter Ricardo; Gardenal, Cristina Noemi; (2022): Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/176475 CONICET Digital CONICET
url	http://hdl.handle.net/11336/176475
identifier_str_mv	Laurito, Magdalena; Ayala, Ana Maria; Almiron, Walter Ricardo; Gardenal, Cristina Noemi; (2022): Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/176475 CONICET Digital CONICET
dc.language.none.fl_str_mv	spa
language	spa
dc.relation.none.fl_str_mv	info:eu-repo/grantAgreement/Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica/2013-1254
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa...

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