Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment

Autores
Traglia, German Matias; Furtado, Nicholas; Escalante, Jenny; Almuzara, Marisa; Cittadini, Roxana Marisa; Tuttobene, Marisel Romina; Subils, Tomás; Dominguez Maldonado, Carolina; Viard, Veronica; Gonzalez, Soledad Estela; Sormani, Maria Ines; Tolmasky, Marcelo E.; Vay, Carlos; Rao, Gauri; Ramirez, Maria Soledad
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Multidrug-resistant Achromobacter xylosoxidans (Ax) is responsible for numerous infections affecting immunocompromised and cystic fibrosis patients. Genetic changes often drive the evolution of antimicrobial resistance. A patient with leukemia infected with a strain, Ax114, susceptible to meropenem, ceftazidime/avibactam, and cefiderocol, was initially treated with meropenem. After this course, a strain resistant to meropenem, Ax115, was isolated. A second course with ceftazidime/avibactam was followed by the isolation of a strain, Ax130, characterized by resistance to ceftazidime/avibactam and reduced susceptibility to cefiderocol. Multilocus sequence typing and phylogenetic analysis using core genes and variant calling analyses revealed that all three isolates were genetically related, suggesting a common origin, and belonged to sequence type 184 (ST184). The mutations potentially most relevant to drug resistance were those identified in the genes penA (one transversion and one transition, Ax130) and eal (one transversion, Ax115 and Ax130). The former gene encodes the peptidoglycan D,D transpeptidase, and the latter codes for a hydrolase that catalyzes c-di-GMP degradation and is associated with virulence and biofilm formation. Strains Ax115 and Ax130 acquired a class 1 integron that harbors the aadB-blaoxa-2 gene cassette in the variable region. Transcriptomic analysis also showed that blaAXC-1 (intrinsic β-lactamase) expression is higher in Ax115 and Ax130 than in Ax114. The results strongly suggest that the antibiotic treatments primarily selected Ax115 and Ax130.
Fil: Traglia, German Matias. Universidad de la República; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Furtado, Nicholas. California State University; Estados Unidos
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: Almuzara, Marisa. Universidad de Buenos Aires; Argentina
Fil: Cittadini, Roxana Marisa. Sanatorio Mater Dei;
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Dominguez Maldonado, Carolina. California State University; Estados Unidos
Fil: Viard, Veronica. California State University; Estados Unidos
Fil: Gonzalez, Soledad Estela. California State University; Estados Unidos
Fil: Sormani, Maria Ines. California State University; Estados Unidos
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Fil: Vay, Carlos. Universidad de Buenos Aires; Argentina
Fil: Rao, Gauri. California State University; Estados Unidos
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ACHROMOBACTER
LEUKEMIA
ANTIBIOTIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267119
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/267119
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatmentTraglia, German MatiasFurtado, NicholasEscalante, JennyAlmuzara, MarisaCittadini, Roxana MarisaTuttobene, Marisel RominaSubils, TomásDominguez Maldonado, CarolinaViard, VeronicaGonzalez, Soledad EstelaSormani, Maria InesTolmasky, Marcelo E.Vay, CarlosRao, GauriRamirez, Maria SoledadACHROMOBACTERLEUKEMIAANTIBIOTIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Multidrug-resistant Achromobacter xylosoxidans (Ax) is responsible for numerous infections affecting immunocompromised and cystic fibrosis patients. Genetic changes often drive the evolution of antimicrobial resistance. A patient with leukemia infected with a strain, Ax114, susceptible to meropenem, ceftazidime/avibactam, and cefiderocol, was initially treated with meropenem. After this course, a strain resistant to meropenem, Ax115, was isolated. A second course with ceftazidime/avibactam was followed by the isolation of a strain, Ax130, characterized by resistance to ceftazidime/avibactam and reduced susceptibility to cefiderocol. Multilocus sequence typing and phylogenetic analysis using core genes and variant calling analyses revealed that all three isolates were genetically related, suggesting a common origin, and belonged to sequence type 184 (ST184). The mutations potentially most relevant to drug resistance were those identified in the genes penA (one transversion and one transition, Ax130) and eal (one transversion, Ax115 and Ax130). The former gene encodes the peptidoglycan D,D transpeptidase, and the latter codes for a hydrolase that catalyzes c-di-GMP degradation and is associated with virulence and biofilm formation. Strains Ax115 and Ax130 acquired a class 1 integron that harbors the aadB-blaoxa-2 gene cassette in the variable region. Transcriptomic analysis also showed that blaAXC-1 (intrinsic β-lactamase) expression is higher in Ax115 and Ax130 than in Ax114. The results strongly suggest that the antibiotic treatments primarily selected Ax115 and Ax130.Fil: Traglia, German Matias. Universidad de la República; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Furtado, Nicholas. California State University; Estados UnidosFil: Escalante, Jenny. California State University; Estados UnidosFil: Almuzara, Marisa. Universidad de Buenos Aires; ArgentinaFil: Cittadini, Roxana Marisa. Sanatorio Mater Dei;Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Dominguez Maldonado, Carolina. California State University; Estados UnidosFil: Viard, Veronica. California State University; Estados UnidosFil: Gonzalez, Soledad Estela. California State University; Estados UnidosFil: Sormani, Maria Ines. California State University; Estados UnidosFil: Tolmasky, Marcelo E.. California State University; Estados UnidosFil: Vay, Carlos. Universidad de Buenos Aires; ArgentinaFil: Rao, Gauri. California State University; Estados UnidosFil: Ramirez, Maria Soledad. California State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267119Traglia, German Matias; Furtado, Nicholas; Escalante, Jenny; Almuzara, Marisa; Cittadini, Roxana Marisa; et al.; Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment; Elsevier Science; International Journal of Antimicrobial Agents; 64; 2; 5-2024; 1-60924-8579CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijantimicag.2024.107218info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0924857924001365info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:41:17Zoai:ri.conicet.gov.ar:11336/267119instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:41:18.069CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment
title Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment
spellingShingle Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment
Traglia, German Matias
ACHROMOBACTER
LEUKEMIA
ANTIBIOTIC
title_short Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment
title_full Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment
title_fullStr Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment
title_full_unstemmed Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment
title_sort Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment
dc.creator.none.fl_str_mv Traglia, German Matias
Furtado, Nicholas
Escalante, Jenny
Almuzara, Marisa
Cittadini, Roxana Marisa
Tuttobene, Marisel Romina
Subils, Tomás
Dominguez Maldonado, Carolina
Viard, Veronica
Gonzalez, Soledad Estela
Sormani, Maria Ines
Tolmasky, Marcelo E.
Vay, Carlos
Rao, Gauri
Ramirez, Maria Soledad
author Traglia, German Matias
author_facet Traglia, German Matias
Furtado, Nicholas
Escalante, Jenny
Almuzara, Marisa
Cittadini, Roxana Marisa
Tuttobene, Marisel Romina
Subils, Tomás
Dominguez Maldonado, Carolina
Viard, Veronica
Gonzalez, Soledad Estela
Sormani, Maria Ines
Tolmasky, Marcelo E.
Vay, Carlos
Rao, Gauri
Ramirez, Maria Soledad
author_role author
author2 Furtado, Nicholas
Escalante, Jenny
Almuzara, Marisa
Cittadini, Roxana Marisa
Tuttobene, Marisel Romina
Subils, Tomás
Dominguez Maldonado, Carolina
Viard, Veronica
Gonzalez, Soledad Estela
Sormani, Maria Ines
Tolmasky, Marcelo E.
Vay, Carlos
Rao, Gauri
Ramirez, Maria Soledad
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACHROMOBACTER
LEUKEMIA
ANTIBIOTIC
topic ACHROMOBACTER
LEUKEMIA
ANTIBIOTIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Multidrug-resistant Achromobacter xylosoxidans (Ax) is responsible for numerous infections affecting immunocompromised and cystic fibrosis patients. Genetic changes often drive the evolution of antimicrobial resistance. A patient with leukemia infected with a strain, Ax114, susceptible to meropenem, ceftazidime/avibactam, and cefiderocol, was initially treated with meropenem. After this course, a strain resistant to meropenem, Ax115, was isolated. A second course with ceftazidime/avibactam was followed by the isolation of a strain, Ax130, characterized by resistance to ceftazidime/avibactam and reduced susceptibility to cefiderocol. Multilocus sequence typing and phylogenetic analysis using core genes and variant calling analyses revealed that all three isolates were genetically related, suggesting a common origin, and belonged to sequence type 184 (ST184). The mutations potentially most relevant to drug resistance were those identified in the genes penA (one transversion and one transition, Ax130) and eal (one transversion, Ax115 and Ax130). The former gene encodes the peptidoglycan D,D transpeptidase, and the latter codes for a hydrolase that catalyzes c-di-GMP degradation and is associated with virulence and biofilm formation. Strains Ax115 and Ax130 acquired a class 1 integron that harbors the aadB-blaoxa-2 gene cassette in the variable region. Transcriptomic analysis also showed that blaAXC-1 (intrinsic β-lactamase) expression is higher in Ax115 and Ax130 than in Ax114. The results strongly suggest that the antibiotic treatments primarily selected Ax115 and Ax130.
Fil: Traglia, German Matias. Universidad de la República; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Furtado, Nicholas. California State University; Estados Unidos
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: Almuzara, Marisa. Universidad de Buenos Aires; Argentina
Fil: Cittadini, Roxana Marisa. Sanatorio Mater Dei;
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Dominguez Maldonado, Carolina. California State University; Estados Unidos
Fil: Viard, Veronica. California State University; Estados Unidos
Fil: Gonzalez, Soledad Estela. California State University; Estados Unidos
Fil: Sormani, Maria Ines. California State University; Estados Unidos
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Fil: Vay, Carlos. Universidad de Buenos Aires; Argentina
Fil: Rao, Gauri. California State University; Estados Unidos
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Multidrug-resistant Achromobacter xylosoxidans (Ax) is responsible for numerous infections affecting immunocompromised and cystic fibrosis patients. Genetic changes often drive the evolution of antimicrobial resistance. A patient with leukemia infected with a strain, Ax114, susceptible to meropenem, ceftazidime/avibactam, and cefiderocol, was initially treated with meropenem. After this course, a strain resistant to meropenem, Ax115, was isolated. A second course with ceftazidime/avibactam was followed by the isolation of a strain, Ax130, characterized by resistance to ceftazidime/avibactam and reduced susceptibility to cefiderocol. Multilocus sequence typing and phylogenetic analysis using core genes and variant calling analyses revealed that all three isolates were genetically related, suggesting a common origin, and belonged to sequence type 184 (ST184). The mutations potentially most relevant to drug resistance were those identified in the genes penA (one transversion and one transition, Ax130) and eal (one transversion, Ax115 and Ax130). The former gene encodes the peptidoglycan D,D transpeptidase, and the latter codes for a hydrolase that catalyzes c-di-GMP degradation and is associated with virulence and biofilm formation. Strains Ax115 and Ax130 acquired a class 1 integron that harbors the aadB-blaoxa-2 gene cassette in the variable region. Transcriptomic analysis also showed that blaAXC-1 (intrinsic β-lactamase) expression is higher in Ax115 and Ax130 than in Ax114. The results strongly suggest that the antibiotic treatments primarily selected Ax115 and Ax130.
publishDate 2024
dc.date.none.fl_str_mv 2024-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267119
Traglia, German Matias; Furtado, Nicholas; Escalante, Jenny; Almuzara, Marisa; Cittadini, Roxana Marisa; et al.; Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment; Elsevier Science; International Journal of Antimicrobial Agents; 64; 2; 5-2024; 1-6
0924-8579
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267119
identifier_str_mv Traglia, German Matias; Furtado, Nicholas; Escalante, Jenny; Almuzara, Marisa; Cittadini, Roxana Marisa; et al.; Dynamic evolution of Achromobacter xylosoxydans in a patient with leukemia receiving antibiotic treatment; Elsevier Science; International Journal of Antimicrobial Agents; 64; 2; 5-2024; 1-6
0924-8579
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijantimicag.2024.107218
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0924857924001365
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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