GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury

Autores
Uranga, Romina Maria; Giusto, Norma Maria; Salvador, Gabriela Alejandra
Año de publicación
2008
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Iron induced oxidative injury is comparable to that of ß-amyloid peptide on the brain of Alzheimer’s disease patients. Our purpose was to evaluate the state of PI3K pathway and its downstream effectors Akt and GSK3ß in cerebral cortex synaptosomes exposed 2+ to Fe (50 µM) for different periods of time (5, 30 and 60 min). The increase in Akt phosphorylation in serine 473 and threonine 308 was temporally coincident with PI3K activation (5 min). GSK3ß, the downstream effector of Akt, was also phosphorylated after 5 and 30 min of iron exposure and this phosphorylation was inhibited by LY294002. Additionally, Erk activation was also observed after 5 and 30 min of insult exposure, and this activation was PI3Kdependent. Immunoprecipitations carried out with anti-cSrc demonstrated a strong association between activated Akt and this tyrosine kinase induced by oxidative stress. Our results demonstrate that oxidative stress triggers the activation of different synaptic signaling pathways that operate downstream PI3K/Akt
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
OXIDATIVE STRESS
PI3K
ERK
SYNAPTIC ENDINGS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242651
Acceder
id CONICETDig_75efa7720bb8ed2f24180345e455afa1
oai_identifier_str oai:ri.conicet.gov.ar:11336/242651
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injuryUranga, Romina MariaGiusto, Norma MariaSalvador, Gabriela AlejandraOXIDATIVE STRESSPI3KERKSYNAPTIC ENDINGShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Iron induced oxidative injury is comparable to that of ß-amyloid peptide on the brain of Alzheimer’s disease patients. Our purpose was to evaluate the state of PI3K pathway and its downstream effectors Akt and GSK3ß in cerebral cortex synaptosomes exposed 2+ to Fe (50 µM) for different periods of time (5, 30 and 60 min). The increase in Akt phosphorylation in serine 473 and threonine 308 was temporally coincident with PI3K activation (5 min). GSK3ß, the downstream effector of Akt, was also phosphorylated after 5 and 30 min of iron exposure and this phosphorylation was inhibited by LY294002. Additionally, Erk activation was also observed after 5 and 30 min of insult exposure, and this activation was PI3Kdependent. Immunoprecipitations carried out with anti-cSrc demonstrated a strong association between activated Akt and this tyrosine kinase induced by oxidative stress. Our results demonstrate that oxidative stress triggers the activation of different synaptic signaling pathways that operate downstream PI3K/AktFil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularVilla Carlos PazArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularInstituto de Histología y Embriología2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242651GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury; XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Villa Carlos Paz; Argentina; 2008; 120-1200327-95451667-5746CONICET DigitalCONICETengNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:04Zoai:ri.conicet.gov.ar:11336/242651instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:05.015CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury
title GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury
spellingShingle GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury
Uranga, Romina Maria
OXIDATIVE STRESS
PI3K
ERK
SYNAPTIC ENDINGS
title_short GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury
title_full GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury
title_fullStr GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury
title_full_unstemmed GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury
title_sort GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury
dc.creator.none.fl_str_mv Uranga, Romina Maria
Giusto, Norma Maria
Salvador, Gabriela Alejandra
author Uranga, Romina Maria
author_facet Uranga, Romina Maria
Giusto, Norma Maria
Salvador, Gabriela Alejandra
author_role author
author2 Giusto, Norma Maria
Salvador, Gabriela Alejandra
author2_role author
author
dc.subject.none.fl_str_mv OXIDATIVE STRESS
PI3K
ERK
SYNAPTIC ENDINGS
topic OXIDATIVE STRESS
PI3K
ERK
SYNAPTIC ENDINGS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Iron induced oxidative injury is comparable to that of ß-amyloid peptide on the brain of Alzheimer’s disease patients. Our purpose was to evaluate the state of PI3K pathway and its downstream effectors Akt and GSK3ß in cerebral cortex synaptosomes exposed 2+ to Fe (50 µM) for different periods of time (5, 30 and 60 min). The increase in Akt phosphorylation in serine 473 and threonine 308 was temporally coincident with PI3K activation (5 min). GSK3ß, the downstream effector of Akt, was also phosphorylated after 5 and 30 min of iron exposure and this phosphorylation was inhibited by LY294002. Additionally, Erk activation was also observed after 5 and 30 min of insult exposure, and this activation was PI3Kdependent. Immunoprecipitations carried out with anti-cSrc demonstrated a strong association between activated Akt and this tyrosine kinase induced by oxidative stress. Our results demonstrate that oxidative stress triggers the activation of different synaptic signaling pathways that operate downstream PI3K/Akt
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Iron induced oxidative injury is comparable to that of ß-amyloid peptide on the brain of Alzheimer’s disease patients. Our purpose was to evaluate the state of PI3K pathway and its downstream effectors Akt and GSK3ß in cerebral cortex synaptosomes exposed 2+ to Fe (50 µM) for different periods of time (5, 30 and 60 min). The increase in Akt phosphorylation in serine 473 and threonine 308 was temporally coincident with PI3K activation (5 min). GSK3ß, the downstream effector of Akt, was also phosphorylated after 5 and 30 min of iron exposure and this phosphorylation was inhibited by LY294002. Additionally, Erk activation was also observed after 5 and 30 min of insult exposure, and this activation was PI3Kdependent. Immunoprecipitations carried out with anti-cSrc demonstrated a strong association between activated Akt and this tyrosine kinase induced by oxidative stress. Our results demonstrate that oxidative stress triggers the activation of different synaptic signaling pathways that operate downstream PI3K/Akt
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242651
GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury; XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Villa Carlos Paz; Argentina; 2008; 120-120
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242651
identifier_str_mv GSK3 beta and Erk are activated downstream of PI3K in synaptic endings during oxidative injury; XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Villa Carlos Paz; Argentina; 2008; 120-120
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614489600688128
score 13.070432

Publicaciones similares