Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components

Autores
Herrera Lopez, Malena; Bertone Arolfo, Matías; Remedi, Maria Monica; Gastaldi, Laura Micaela; Cerqueira Matos, Thiara; Guendulain, Gonzalo Germán; Ceschin, Danilo Guillermo; Cardozo Gizzi, Andres Mauricio; Caceres, Alfredo Oscar; Moyano, Ana Lis
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Extracellular vesicles (EVs) play a critical role in the development of neural cells in the central nervous system (CNS). Human neural rosettes (hNRs) are radial cell structures that assemble from induced pluripotent stem cells (hiPSCs) and recapitulate some stages of neural tube morphogenesis. Here we show that hiPSCs and hNRs secrete EVs (hiPSC-EVs and hNR-EVs) with distinctive protein cargoes. Remarkably, hNR-EVs carry neuronal and glial cellular components involved in human CNS development. Importantly, hNR-EVs stimulate stem cells to change their cellular morphology and promote neurite growth in human and murine neurons with a significant dysregulation of SOX2 levels. This transcription factor modulates both neural differentiation and pluripotency. Interestingly, these effects were inhibited by antibodies against an unexpected neuroglial cargo of hNR-EVs: the major proteolipid protein (PLP). These findings show that hNRs secrete bioactive EVs containing neural components and might contribute as trophic factors during human neurodevelopment.
Fil: Herrera Lopez, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bertone Arolfo, Matías. No especifíca;
Fil: Remedi, Maria Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gastaldi, Laura Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cerqueira Matos, Thiara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guendulain, Gonzalo Germán. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceschin, Danilo Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardozo Gizzi, Andres Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caceres, Alfredo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moyano, Ana Lis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CNS
Neurodevelopment
Extracellular vesicles
Stem cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/282267
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/282267
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular componentsHerrera Lopez, MalenaBertone Arolfo, MatíasRemedi, Maria MonicaGastaldi, Laura MicaelaCerqueira Matos, ThiaraGuendulain, Gonzalo GermánCeschin, Danilo GuillermoCardozo Gizzi, Andres MauricioCaceres, Alfredo OscarMoyano, Ana LisCNSNeurodevelopmentExtracellular vesiclesStem cellshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Extracellular vesicles (EVs) play a critical role in the development of neural cells in the central nervous system (CNS). Human neural rosettes (hNRs) are radial cell structures that assemble from induced pluripotent stem cells (hiPSCs) and recapitulate some stages of neural tube morphogenesis. Here we show that hiPSCs and hNRs secrete EVs (hiPSC-EVs and hNR-EVs) with distinctive protein cargoes. Remarkably, hNR-EVs carry neuronal and glial cellular components involved in human CNS development. Importantly, hNR-EVs stimulate stem cells to change their cellular morphology and promote neurite growth in human and murine neurons with a significant dysregulation of SOX2 levels. This transcription factor modulates both neural differentiation and pluripotency. Interestingly, these effects were inhibited by antibodies against an unexpected neuroglial cargo of hNR-EVs: the major proteolipid protein (PLP). These findings show that hNRs secrete bioactive EVs containing neural components and might contribute as trophic factors during human neurodevelopment.Fil: Herrera Lopez, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bertone Arolfo, Matías. No especifíca;Fil: Remedi, Maria Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gastaldi, Laura Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cerqueira Matos, Thiara. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guendulain, Gonzalo Germán. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ceschin, Danilo Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cardozo Gizzi, Andres Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Caceres, Alfredo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moyano, Ana Lis. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/282267Herrera Lopez, Malena; Bertone Arolfo, Matías; Remedi, Maria Monica; Gastaldi, Laura Micaela; Cerqueira Matos, Thiara; et al.; Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components; Springer; Scientific Reports; 15; 1; 1-2025; 1-132045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-025-86094-9info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-025-86094-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-11T12:19:11Zoai:ri.conicet.gov.ar:11336/282267instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-11 12:19:11.658CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components
title Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components
spellingShingle Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components
Herrera Lopez, Malena
CNS
Neurodevelopment
Extracellular vesicles
Stem cells
title_short Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components
title_full Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components
title_fullStr Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components
title_full_unstemmed Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components
title_sort Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components
dc.creator.none.fl_str_mv Herrera Lopez, Malena
Bertone Arolfo, Matías
Remedi, Maria Monica
Gastaldi, Laura Micaela
Cerqueira Matos, Thiara
Guendulain, Gonzalo Germán
Ceschin, Danilo Guillermo
Cardozo Gizzi, Andres Mauricio
Caceres, Alfredo Oscar
Moyano, Ana Lis
author Herrera Lopez, Malena
author_facet Herrera Lopez, Malena
Bertone Arolfo, Matías
Remedi, Maria Monica
Gastaldi, Laura Micaela
Cerqueira Matos, Thiara
Guendulain, Gonzalo Germán
Ceschin, Danilo Guillermo
Cardozo Gizzi, Andres Mauricio
Caceres, Alfredo Oscar
Moyano, Ana Lis
author_role author
author2 Bertone Arolfo, Matías
Remedi, Maria Monica
Gastaldi, Laura Micaela
Cerqueira Matos, Thiara
Guendulain, Gonzalo Germán
Ceschin, Danilo Guillermo
Cardozo Gizzi, Andres Mauricio
Caceres, Alfredo Oscar
Moyano, Ana Lis
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CNS
Neurodevelopment
Extracellular vesicles
Stem cells
topic CNS
Neurodevelopment
Extracellular vesicles
Stem cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Extracellular vesicles (EVs) play a critical role in the development of neural cells in the central nervous system (CNS). Human neural rosettes (hNRs) are radial cell structures that assemble from induced pluripotent stem cells (hiPSCs) and recapitulate some stages of neural tube morphogenesis. Here we show that hiPSCs and hNRs secrete EVs (hiPSC-EVs and hNR-EVs) with distinctive protein cargoes. Remarkably, hNR-EVs carry neuronal and glial cellular components involved in human CNS development. Importantly, hNR-EVs stimulate stem cells to change their cellular morphology and promote neurite growth in human and murine neurons with a significant dysregulation of SOX2 levels. This transcription factor modulates both neural differentiation and pluripotency. Interestingly, these effects were inhibited by antibodies against an unexpected neuroglial cargo of hNR-EVs: the major proteolipid protein (PLP). These findings show that hNRs secrete bioactive EVs containing neural components and might contribute as trophic factors during human neurodevelopment.
Fil: Herrera Lopez, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bertone Arolfo, Matías. No especifíca;
Fil: Remedi, Maria Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gastaldi, Laura Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cerqueira Matos, Thiara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guendulain, Gonzalo Germán. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceschin, Danilo Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardozo Gizzi, Andres Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caceres, Alfredo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moyano, Ana Lis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Extracellular vesicles (EVs) play a critical role in the development of neural cells in the central nervous system (CNS). Human neural rosettes (hNRs) are radial cell structures that assemble from induced pluripotent stem cells (hiPSCs) and recapitulate some stages of neural tube morphogenesis. Here we show that hiPSCs and hNRs secrete EVs (hiPSC-EVs and hNR-EVs) with distinctive protein cargoes. Remarkably, hNR-EVs carry neuronal and glial cellular components involved in human CNS development. Importantly, hNR-EVs stimulate stem cells to change their cellular morphology and promote neurite growth in human and murine neurons with a significant dysregulation of SOX2 levels. This transcription factor modulates both neural differentiation and pluripotency. Interestingly, these effects were inhibited by antibodies against an unexpected neuroglial cargo of hNR-EVs: the major proteolipid protein (PLP). These findings show that hNRs secrete bioactive EVs containing neural components and might contribute as trophic factors during human neurodevelopment.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/282267
Herrera Lopez, Malena; Bertone Arolfo, Matías; Remedi, Maria Monica; Gastaldi, Laura Micaela; Cerqueira Matos, Thiara; et al.; Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components; Springer; Scientific Reports; 15; 1; 1-2025; 1-13
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/282267
identifier_str_mv Herrera Lopez, Malena; Bertone Arolfo, Matías; Remedi, Maria Monica; Gastaldi, Laura Micaela; Cerqueira Matos, Thiara; et al.; Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components; Springer; Scientific Reports; 15; 1; 1-2025; 1-13
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-025-86094-9
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-025-86094-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.977003

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