Commelina Platyphylla

Autores
Grabiele, Mauro; Honfi, Ana Isabel; Daviña, Julio Rubén
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Commelina platyphylla is a diploid species widely distributed in South America, representing a convenient model for karyological study. Different classical and molecular cytogenetics techniques were applied in order to evaluate its chromosomes. DAPI staining showed an unimodal and symmetrical karyotype (2A according to Stebbins, 1971) comprising 9m + 6sm medium-size chromosomes ranging from 2.29 to 4.04 µm, and 47.77 µm per haploid genome. In addition, CMA/Distamycin-A/DAPI fluorescence banding revealed the presence of two different types of constitutive heterochromatin (Hc), CMA+ DAPI (GC-rich) and CMA DAPI+ (ATrich), both comprising the 7.08% (3.38 µm) of the haploid genome. GC-rich Hc (42% of total) is exclusively NOR-associated, covering the entire terminal macrosatellite of chromosome pairs nos. 10 (sm) and 13 (sm). AT-rich Hc (58% of total) is localized interstitially (pairs nos. 4 and 12) or found at proximal (pairs nos. 2, 3 and 10) positions. Ag-NOR staining revealed 1 (77%) > 2 (19%) > 3 (3%) > 4 (1%) nucleoli in interphase nuclei and 1 (38%) > 2 (35%) > 3 (15%) > 4 (12%) active nucleolar organizer regions (NORs) in metaphase; nucleolar dominance of pair no. 10 vs. 13 (1.6 times) is observed by classical staining and confirmed by Ag-NOR and also nucleolar associated bodies (NABs) were recognized. Polymorphisms for cytological markers were not observed. The equilocal distribution of the different Hc blocks and NORs, its arrangement within the classical staining predicted suprachromosomal organization according to Bennett´s model added to the disposition of the chromocenters and the NABs in the interphase nuclei suggest concerted evolution for the Hc and NORs dispersion in C. platyphylla.
Fil: Grabiele, Mauro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Laboratorio de Citogenética Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Honfi, Ana Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Laboratorio de Citogenética Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Daviña, Julio Rubén. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Laboratorio de Citogenética Vegetal; Argentina
Materia
Karyotype
Heterochromatin
Nors
Concerted Evolution
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43474
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43474
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Commelina PlatyphyllaGrabiele, MauroHonfi, Ana IsabelDaviña, Julio RubénKaryotypeHeterochromatinNorsConcerted Evolutionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Commelina platyphylla is a diploid species widely distributed in South America, representing a convenient model for karyological study. Different classical and molecular cytogenetics techniques were applied in order to evaluate its chromosomes. DAPI staining showed an unimodal and symmetrical karyotype (2A according to Stebbins, 1971) comprising 9m + 6sm medium-size chromosomes ranging from 2.29 to 4.04 µm, and 47.77 µm per haploid genome. In addition, CMA/Distamycin-A/DAPI fluorescence banding revealed the presence of two different types of constitutive heterochromatin (Hc), CMA+ DAPI (GC-rich) and CMA DAPI+ (ATrich), both comprising the 7.08% (3.38 µm) of the haploid genome. GC-rich Hc (42% of total) is exclusively NOR-associated, covering the entire terminal macrosatellite of chromosome pairs nos. 10 (sm) and 13 (sm). AT-rich Hc (58% of total) is localized interstitially (pairs nos. 4 and 12) or found at proximal (pairs nos. 2, 3 and 10) positions. Ag-NOR staining revealed 1 (77%) > 2 (19%) > 3 (3%) > 4 (1%) nucleoli in interphase nuclei and 1 (38%) > 2 (35%) > 3 (15%) > 4 (12%) active nucleolar organizer regions (NORs) in metaphase; nucleolar dominance of pair no. 10 vs. 13 (1.6 times) is observed by classical staining and confirmed by Ag-NOR and also nucleolar associated bodies (NABs) were recognized. Polymorphisms for cytological markers were not observed. The equilocal distribution of the different Hc blocks and NORs, its arrangement within the classical staining predicted suprachromosomal organization according to Bennett´s model added to the disposition of the chromocenters and the NABs in the interphase nuclei suggest concerted evolution for the Hc and NORs dispersion in C. platyphylla.Fil: Grabiele, Mauro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Laboratorio de Citogenética Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Honfi, Ana Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Laboratorio de Citogenética Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Daviña, Julio Rubén. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Laboratorio de Citogenética Vegetal; ArgentinaInternational Association for Plant Taxonomy2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43474Grabiele, Mauro; Honfi, Ana Isabel; Daviña, Julio Rubén; Commelina Platyphylla; International Association for Plant Taxonomy; Taxon; 59; 6; 12-2010; 1-20040-0262CONICET DigitalCONICETengMarhold, Karol (ed). IAPT/IOPB chromosome data 10info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/contentone/iapt/tax/2010/00000059/00000006/art00056info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:02Zoai:ri.conicet.gov.ar:11336/43474instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:03.1CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Commelina Platyphylla
title Commelina Platyphylla
spellingShingle Commelina Platyphylla
Grabiele, Mauro
Karyotype
Heterochromatin
Nors
Concerted Evolution
title_short Commelina Platyphylla
title_full Commelina Platyphylla
title_fullStr Commelina Platyphylla
title_full_unstemmed Commelina Platyphylla
title_sort Commelina Platyphylla
dc.creator.none.fl_str_mv Grabiele, Mauro
Honfi, Ana Isabel
Daviña, Julio Rubén
author Grabiele, Mauro
author_facet Grabiele, Mauro
Honfi, Ana Isabel
Daviña, Julio Rubén
author_role author
author2 Honfi, Ana Isabel
Daviña, Julio Rubén
author2_role author
author
dc.subject.none.fl_str_mv Karyotype
Heterochromatin
Nors
Concerted Evolution
topic Karyotype
Heterochromatin
Nors
Concerted Evolution
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Commelina platyphylla is a diploid species widely distributed in South America, representing a convenient model for karyological study. Different classical and molecular cytogenetics techniques were applied in order to evaluate its chromosomes. DAPI staining showed an unimodal and symmetrical karyotype (2A according to Stebbins, 1971) comprising 9m + 6sm medium-size chromosomes ranging from 2.29 to 4.04 µm, and 47.77 µm per haploid genome. In addition, CMA/Distamycin-A/DAPI fluorescence banding revealed the presence of two different types of constitutive heterochromatin (Hc), CMA+ DAPI (GC-rich) and CMA DAPI+ (ATrich), both comprising the 7.08% (3.38 µm) of the haploid genome. GC-rich Hc (42% of total) is exclusively NOR-associated, covering the entire terminal macrosatellite of chromosome pairs nos. 10 (sm) and 13 (sm). AT-rich Hc (58% of total) is localized interstitially (pairs nos. 4 and 12) or found at proximal (pairs nos. 2, 3 and 10) positions. Ag-NOR staining revealed 1 (77%) > 2 (19%) > 3 (3%) > 4 (1%) nucleoli in interphase nuclei and 1 (38%) > 2 (35%) > 3 (15%) > 4 (12%) active nucleolar organizer regions (NORs) in metaphase; nucleolar dominance of pair no. 10 vs. 13 (1.6 times) is observed by classical staining and confirmed by Ag-NOR and also nucleolar associated bodies (NABs) were recognized. Polymorphisms for cytological markers were not observed. The equilocal distribution of the different Hc blocks and NORs, its arrangement within the classical staining predicted suprachromosomal organization according to Bennett´s model added to the disposition of the chromocenters and the NABs in the interphase nuclei suggest concerted evolution for the Hc and NORs dispersion in C. platyphylla.
Fil: Grabiele, Mauro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Laboratorio de Citogenética Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Honfi, Ana Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Laboratorio de Citogenética Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Daviña, Julio Rubén. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Laboratorio de Citogenética Vegetal; Argentina
description Commelina platyphylla is a diploid species widely distributed in South America, representing a convenient model for karyological study. Different classical and molecular cytogenetics techniques were applied in order to evaluate its chromosomes. DAPI staining showed an unimodal and symmetrical karyotype (2A according to Stebbins, 1971) comprising 9m + 6sm medium-size chromosomes ranging from 2.29 to 4.04 µm, and 47.77 µm per haploid genome. In addition, CMA/Distamycin-A/DAPI fluorescence banding revealed the presence of two different types of constitutive heterochromatin (Hc), CMA+ DAPI (GC-rich) and CMA DAPI+ (ATrich), both comprising the 7.08% (3.38 µm) of the haploid genome. GC-rich Hc (42% of total) is exclusively NOR-associated, covering the entire terminal macrosatellite of chromosome pairs nos. 10 (sm) and 13 (sm). AT-rich Hc (58% of total) is localized interstitially (pairs nos. 4 and 12) or found at proximal (pairs nos. 2, 3 and 10) positions. Ag-NOR staining revealed 1 (77%) > 2 (19%) > 3 (3%) > 4 (1%) nucleoli in interphase nuclei and 1 (38%) > 2 (35%) > 3 (15%) > 4 (12%) active nucleolar organizer regions (NORs) in metaphase; nucleolar dominance of pair no. 10 vs. 13 (1.6 times) is observed by classical staining and confirmed by Ag-NOR and also nucleolar associated bodies (NABs) were recognized. Polymorphisms for cytological markers were not observed. The equilocal distribution of the different Hc blocks and NORs, its arrangement within the classical staining predicted suprachromosomal organization according to Bennett´s model added to the disposition of the chromocenters and the NABs in the interphase nuclei suggest concerted evolution for the Hc and NORs dispersion in C. platyphylla.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43474
Grabiele, Mauro; Honfi, Ana Isabel; Daviña, Julio Rubén; Commelina Platyphylla; International Association for Plant Taxonomy; Taxon; 59; 6; 12-2010; 1-2
0040-0262
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43474
identifier_str_mv Grabiele, Mauro; Honfi, Ana Isabel; Daviña, Julio Rubén; Commelina Platyphylla; International Association for Plant Taxonomy; Taxon; 59; 6; 12-2010; 1-2
0040-0262
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Marhold, Karol (ed). IAPT/IOPB chromosome data 10
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/contentone/iapt/tax/2010/00000059/00000006/art00056
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Association for Plant Taxonomy
publisher.none.fl_str_mv International Association for Plant Taxonomy
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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