The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis

Autores
Pescaretti, María de Las Mercedes; Lopez, Fabian Enrique; Morero, Roberto Dionisio; Delgado, Monica Alejandra
Año de publicación
2009
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The Rcs phosphorelay is a adaptive response system composed of three proteins: the sensor RcsC, the cognate response regulator RcsB; and the intermediary in the phosphoryl transfer RcsD. Has been described that the flow of phosphoryl groups through the Rcs phosphorelay can occur in following way RcsC/RcsD/RcsB. At present, the signal that activates the Rcs system has not been determined. We have demonstrated in a previous study that the overexpression of rcsB modulates the RcsB-dependent genes in an rcsC or rcsD mutant but not in the doble mutant. These results suggest that only RcsB-P, the RcsB phosphorylated form, is able to induce this modulation, and we postulated that the RcsB-P would occur by two or more different pathways. The goal of present work was study the possible phosphorelay pathways. In this work we used the two-hybrid assay (BACTH), consisting of the reconstitution of the adenylate cyclase activity in E. coli when two target proteins interacts in vivo in bacteria, measured by b-galactosidase activity. In this assay we probe the RcsC, RcsD and RcsB interaction to define the Rcs phosphorelay transduction mechanism.
Fil: Pescaretti, María de Las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Lopez, Fabian Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Morero, Roberto Dionisio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
San Miguel de Tucumán
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Materia
RCSCDB
SYSTEM
PROTEIN
INTERACTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195008
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195008
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysisPescaretti, María de Las MercedesLopez, Fabian EnriqueMorero, Roberto DionisioDelgado, Monica AlejandraRCSCDBSYSTEMPROTEININTERACTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Rcs phosphorelay is a adaptive response system composed of three proteins: the sensor RcsC, the cognate response regulator RcsB; and the intermediary in the phosphoryl transfer RcsD. Has been described that the flow of phosphoryl groups through the Rcs phosphorelay can occur in following way RcsC/RcsD/RcsB. At present, the signal that activates the Rcs system has not been determined. We have demonstrated in a previous study that the overexpression of rcsB modulates the RcsB-dependent genes in an rcsC or rcsD mutant but not in the doble mutant. These results suggest that only RcsB-P, the RcsB phosphorylated form, is able to induce this modulation, and we postulated that the RcsB-P would occur by two or more different pathways. The goal of present work was study the possible phosphorelay pathways. In this work we used the two-hybrid assay (BACTH), consisting of the reconstitution of the adenylate cyclase activity in E. coli when two target proteins interacts in vivo in bacteria, measured by b-galactosidase activity. In this assay we probe the RcsC, RcsD and RcsB interaction to define the Rcs phosphorelay transduction mechanism.Fil: Pescaretti, María de Las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Lopez, Fabian Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Morero, Roberto Dionisio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaXLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología MolecularSan Miguel de TucumánArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularTech Science Press2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195008The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis; XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; San Miguel de Tucumán; Argentina; 2009; 100-1000327-95451667-5746CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v33nSuppl.S/38002Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:22Zoai:ri.conicet.gov.ar:11336/195008instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:22.707CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis
title The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis
spellingShingle The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis
Pescaretti, María de Las Mercedes
RCSCDB
SYSTEM
PROTEIN
INTERACTION
title_short The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis
title_full The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis
title_fullStr The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis
title_full_unstemmed The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis
title_sort The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis
dc.creator.none.fl_str_mv Pescaretti, María de Las Mercedes
Lopez, Fabian Enrique
Morero, Roberto Dionisio
Delgado, Monica Alejandra
author Pescaretti, María de Las Mercedes
author_facet Pescaretti, María de Las Mercedes
Lopez, Fabian Enrique
Morero, Roberto Dionisio
Delgado, Monica Alejandra
author_role author
author2 Lopez, Fabian Enrique
Morero, Roberto Dionisio
Delgado, Monica Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv RCSCDB
SYSTEM
PROTEIN
INTERACTION
topic RCSCDB
SYSTEM
PROTEIN
INTERACTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Rcs phosphorelay is a adaptive response system composed of three proteins: the sensor RcsC, the cognate response regulator RcsB; and the intermediary in the phosphoryl transfer RcsD. Has been described that the flow of phosphoryl groups through the Rcs phosphorelay can occur in following way RcsC/RcsD/RcsB. At present, the signal that activates the Rcs system has not been determined. We have demonstrated in a previous study that the overexpression of rcsB modulates the RcsB-dependent genes in an rcsC or rcsD mutant but not in the doble mutant. These results suggest that only RcsB-P, the RcsB phosphorylated form, is able to induce this modulation, and we postulated that the RcsB-P would occur by two or more different pathways. The goal of present work was study the possible phosphorelay pathways. In this work we used the two-hybrid assay (BACTH), consisting of the reconstitution of the adenylate cyclase activity in E. coli when two target proteins interacts in vivo in bacteria, measured by b-galactosidase activity. In this assay we probe the RcsC, RcsD and RcsB interaction to define the Rcs phosphorelay transduction mechanism.
Fil: Pescaretti, María de Las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Lopez, Fabian Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Morero, Roberto Dionisio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
San Miguel de Tucumán
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
description The Rcs phosphorelay is a adaptive response system composed of three proteins: the sensor RcsC, the cognate response regulator RcsB; and the intermediary in the phosphoryl transfer RcsD. Has been described that the flow of phosphoryl groups through the Rcs phosphorelay can occur in following way RcsC/RcsD/RcsB. At present, the signal that activates the Rcs system has not been determined. We have demonstrated in a previous study that the overexpression of rcsB modulates the RcsB-dependent genes in an rcsC or rcsD mutant but not in the doble mutant. These results suggest that only RcsB-P, the RcsB phosphorylated form, is able to induce this modulation, and we postulated that the RcsB-P would occur by two or more different pathways. The goal of present work was study the possible phosphorelay pathways. In this work we used the two-hybrid assay (BACTH), consisting of the reconstitution of the adenylate cyclase activity in E. coli when two target proteins interacts in vivo in bacteria, measured by b-galactosidase activity. In this assay we probe the RcsC, RcsD and RcsB interaction to define the Rcs phosphorelay transduction mechanism.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195008
The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis; XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; San Miguel de Tucumán; Argentina; 2009; 100-100
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195008
identifier_str_mv The RcsC/RcsD/RcsB system in Salmonella typhimurium: a bacterial two-hybrid analysis; XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; San Miguel de Tucumán; Argentina; 2009; 100-100
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v33nSuppl.S/38002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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