Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?

Autores
Pacheco, Maria Emilia; Bruzzone, Liliana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Synchronous fluorescence spectrometry has become a common tool to investigate conformational changes in proteins due to the presence of other molecules (quenchers). The same pattern is always followed, leaving aside the possibility of interfering effects that make conclusions incorrect. In this paper, we discuss the main cause of a wrong understanding of synchronous fluorescence spectrometry in a large number of papers published in many different journals. Based on the principles of synchronous fluorescence spectrometry, we perform a simple correction method of synchronous spectra taking into account inner filter effects. This criticism tries to demonstrate that the interpretation of synchronous fluorescence spectrometry is not as easy as it seems.
Fil: Pacheco, Maria Emilia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Quimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bruzzone, Liliana. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Quimica; Argentina
Materia
Synchronous Fluorescence Spectrometry
Inner Filter Effect
Bovine Serum Albumin
Imazethapyr
Chlorimuron-Ethyl
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22862
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22862
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?Pacheco, Maria EmiliaBruzzone, LilianaSynchronous Fluorescence SpectrometryInner Filter EffectBovine Serum AlbuminImazethapyrChlorimuron-Ethylhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Synchronous fluorescence spectrometry has become a common tool to investigate conformational changes in proteins due to the presence of other molecules (quenchers). The same pattern is always followed, leaving aside the possibility of interfering effects that make conclusions incorrect. In this paper, we discuss the main cause of a wrong understanding of synchronous fluorescence spectrometry in a large number of papers published in many different journals. Based on the principles of synchronous fluorescence spectrometry, we perform a simple correction method of synchronous spectra taking into account inner filter effects. This criticism tries to demonstrate that the interpretation of synchronous fluorescence spectrometry is not as easy as it seems.Fil: Pacheco, Maria Emilia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Quimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bruzzone, Liliana. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Quimica; ArgentinaElsevier Science2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22862Pacheco, Maria Emilia; Bruzzone, Liliana; Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?; Elsevier Science; Journal of Luminescence; 137; 1-2013; 138-1420022-2313CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jlumin.2012.12.056info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022231313000021info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:52Zoai:ri.conicet.gov.ar:11336/22862instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:52.338CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
title Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
spellingShingle Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
Pacheco, Maria Emilia
Synchronous Fluorescence Spectrometry
Inner Filter Effect
Bovine Serum Albumin
Imazethapyr
Chlorimuron-Ethyl
title_short Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
title_full Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
title_fullStr Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
title_full_unstemmed Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
title_sort Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
dc.creator.none.fl_str_mv Pacheco, Maria Emilia
Bruzzone, Liliana
author Pacheco, Maria Emilia
author_facet Pacheco, Maria Emilia
Bruzzone, Liliana
author_role author
author2 Bruzzone, Liliana
author2_role author
dc.subject.none.fl_str_mv Synchronous Fluorescence Spectrometry
Inner Filter Effect
Bovine Serum Albumin
Imazethapyr
Chlorimuron-Ethyl
topic Synchronous Fluorescence Spectrometry
Inner Filter Effect
Bovine Serum Albumin
Imazethapyr
Chlorimuron-Ethyl
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Synchronous fluorescence spectrometry has become a common tool to investigate conformational changes in proteins due to the presence of other molecules (quenchers). The same pattern is always followed, leaving aside the possibility of interfering effects that make conclusions incorrect. In this paper, we discuss the main cause of a wrong understanding of synchronous fluorescence spectrometry in a large number of papers published in many different journals. Based on the principles of synchronous fluorescence spectrometry, we perform a simple correction method of synchronous spectra taking into account inner filter effects. This criticism tries to demonstrate that the interpretation of synchronous fluorescence spectrometry is not as easy as it seems.
Fil: Pacheco, Maria Emilia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Quimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bruzzone, Liliana. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Quimica; Argentina
description Synchronous fluorescence spectrometry has become a common tool to investigate conformational changes in proteins due to the presence of other molecules (quenchers). The same pattern is always followed, leaving aside the possibility of interfering effects that make conclusions incorrect. In this paper, we discuss the main cause of a wrong understanding of synchronous fluorescence spectrometry in a large number of papers published in many different journals. Based on the principles of synchronous fluorescence spectrometry, we perform a simple correction method of synchronous spectra taking into account inner filter effects. This criticism tries to demonstrate that the interpretation of synchronous fluorescence spectrometry is not as easy as it seems.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22862
Pacheco, Maria Emilia; Bruzzone, Liliana; Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?; Elsevier Science; Journal of Luminescence; 137; 1-2013; 138-142
0022-2313
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22862
identifier_str_mv Pacheco, Maria Emilia; Bruzzone, Liliana; Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?; Elsevier Science; Journal of Luminescence; 137; 1-2013; 138-142
0022-2313
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jlumin.2012.12.056
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022231313000021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269311862833152
score 13.13397

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