Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors

Autores
Fakhoury, Joseph N.; Capdevila, Daiana Andrea; Giedroc, David Peter
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hydrogen sulfide (H2S) and downstream reactive sulfur species (RSS), including organic persulfides, protect bacterial cells against diverse oxidative stressors. Specialized dithiol-based transcriptional repressors sense persulfides directly to control cellular H2S/RSS and avoid toxicity. Here, we present a protocol to quantify the kinetics of chemical reactivity of cysteines in two bacterial persulfide sensors toward cysteine persulfide and glutathione persulfide, with a LC-ESI-MS analysis that results in a kinetic model. This protocol has potential applications to other cysteine-containing proteins and oxidants. For complete details on the use and execution of this protocol, please refer to Fakhoury et al. (2021) and Capdevila et al. (2021).
Fil: Fakhoury, Joseph N.. Indiana University; Estados Unidos
Fil: Capdevila, Daiana Andrea. Indiana University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Giedroc, David Peter. Indiana University; Estados Unidos
Materia
MASS SPECTROMETRY
PROTEIN BIOCHEMISTRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214353
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/214353
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensorsFakhoury, Joseph N.Capdevila, Daiana AndreaGiedroc, David PeterMASS SPECTROMETRYPROTEIN BIOCHEMISTRYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Hydrogen sulfide (H2S) and downstream reactive sulfur species (RSS), including organic persulfides, protect bacterial cells against diverse oxidative stressors. Specialized dithiol-based transcriptional repressors sense persulfides directly to control cellular H2S/RSS and avoid toxicity. Here, we present a protocol to quantify the kinetics of chemical reactivity of cysteines in two bacterial persulfide sensors toward cysteine persulfide and glutathione persulfide, with a LC-ESI-MS analysis that results in a kinetic model. This protocol has potential applications to other cysteine-containing proteins and oxidants. For complete details on the use and execution of this protocol, please refer to Fakhoury et al. (2021) and Capdevila et al. (2021).Fil: Fakhoury, Joseph N.. Indiana University; Estados UnidosFil: Capdevila, Daiana Andrea. Indiana University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Giedroc, David Peter. Indiana University; Estados UnidosCell Press2022-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214353Fakhoury, Joseph N.; Capdevila, Daiana Andrea; Giedroc, David Peter; Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors; Cell Press; STAR Protocols; 3; 2; 6-2022; 1-132666-1667CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.xpro.2022.101424info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:45Zoai:ri.conicet.gov.ar:11336/214353instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:45.627CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors
title Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors
spellingShingle Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors
Fakhoury, Joseph N.
MASS SPECTROMETRY
PROTEIN BIOCHEMISTRY
title_short Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors
title_full Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors
title_fullStr Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors
title_full_unstemmed Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors
title_sort Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors
dc.creator.none.fl_str_mv Fakhoury, Joseph N.
Capdevila, Daiana Andrea
Giedroc, David Peter
author Fakhoury, Joseph N.
author_facet Fakhoury, Joseph N.
Capdevila, Daiana Andrea
Giedroc, David Peter
author_role author
author2 Capdevila, Daiana Andrea
Giedroc, David Peter
author2_role author
author
dc.subject.none.fl_str_mv MASS SPECTROMETRY
PROTEIN BIOCHEMISTRY
topic MASS SPECTROMETRY
PROTEIN BIOCHEMISTRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hydrogen sulfide (H2S) and downstream reactive sulfur species (RSS), including organic persulfides, protect bacterial cells against diverse oxidative stressors. Specialized dithiol-based transcriptional repressors sense persulfides directly to control cellular H2S/RSS and avoid toxicity. Here, we present a protocol to quantify the kinetics of chemical reactivity of cysteines in two bacterial persulfide sensors toward cysteine persulfide and glutathione persulfide, with a LC-ESI-MS analysis that results in a kinetic model. This protocol has potential applications to other cysteine-containing proteins and oxidants. For complete details on the use and execution of this protocol, please refer to Fakhoury et al. (2021) and Capdevila et al. (2021).
Fil: Fakhoury, Joseph N.. Indiana University; Estados Unidos
Fil: Capdevila, Daiana Andrea. Indiana University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Giedroc, David Peter. Indiana University; Estados Unidos
description Hydrogen sulfide (H2S) and downstream reactive sulfur species (RSS), including organic persulfides, protect bacterial cells against diverse oxidative stressors. Specialized dithiol-based transcriptional repressors sense persulfides directly to control cellular H2S/RSS and avoid toxicity. Here, we present a protocol to quantify the kinetics of chemical reactivity of cysteines in two bacterial persulfide sensors toward cysteine persulfide and glutathione persulfide, with a LC-ESI-MS analysis that results in a kinetic model. This protocol has potential applications to other cysteine-containing proteins and oxidants. For complete details on the use and execution of this protocol, please refer to Fakhoury et al. (2021) and Capdevila et al. (2021).
publishDate 2022
dc.date.none.fl_str_mv 2022-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214353
Fakhoury, Joseph N.; Capdevila, Daiana Andrea; Giedroc, David Peter; Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors; Cell Press; STAR Protocols; 3; 2; 6-2022; 1-13
2666-1667
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214353
identifier_str_mv Fakhoury, Joseph N.; Capdevila, Daiana Andrea; Giedroc, David Peter; Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors; Cell Press; STAR Protocols; 3; 2; 6-2022; 1-13
2666-1667
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.xpro.2022.101424
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614364612526080
score 13.070432

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