Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents

Autores
Garro, Ariel Gustavo; Alasino, Roxana Valeria; Leonhard, Victoria; Heredia, Valeria; Beltramo, Dante Miguel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose: The ability of spermidine to increase the selectivity of anticancer agents has been studied extensively. In this research we report the combination of this polyamine and GM1 ganglioside micelles and characterize their behavior for drug delivery.Methods: Dynamic light scattering and electron microscopy were used to characterize size and morphology of micelles. Zeta potential was determined using a Nano-zeta potential analyzer. Sizeexclusion chromatography was used to separate different populations. Cytotoxic effect of micelles was evaluated on Hep2 cell line.Results: Covalent conjugation of spermidine to gangliosides produces a clear reduction of the electronegative z potential of micelle surface. DLS analysis shows no significant differences between both micelles, while TEM image shows a smaller size of Spermidine-GM1.These modified micelles load hydrophilic or hydrophobic antitumor drugs and conjugation does not affect the stability of micelles/drug in solution. Spermidine-GM1/Doxo micelles show faster drug release into cells as compared with GM1/Doxo micelles; however, no evidence can be found for the participation of the polyamine transport system in the uptake of modified micelles.Conclusion: While Spermidine-GM1 and GM1 micelles show similar physical properties, spermidine modified micelles are most efficient to release drugs, making this an interesting alternative to consider for drug delivery.
Fil: Garro, Ariel Gustavo. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leonhard, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Heredia, Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Beltramo, Dante Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Materia
SPERMIDINE-GM1 MICELLES
POLYAMINES
DRUG TARGETING
CANCER THERAPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/179628
Acceder
id CONICETDig_74c82b615a2eb9b4ca22078748233a13
oai_identifier_str oai:ri.conicet.gov.ar:11336/179628
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral AgentsGarro, Ariel GustavoAlasino, Roxana ValeriaLeonhard, VictoriaHeredia, ValeriaBeltramo, Dante MiguelSPERMIDINE-GM1 MICELLESPOLYAMINESDRUG TARGETINGCANCER THERAPYhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Purpose: The ability of spermidine to increase the selectivity of anticancer agents has been studied extensively. In this research we report the combination of this polyamine and GM1 ganglioside micelles and characterize their behavior for drug delivery.Methods: Dynamic light scattering and electron microscopy were used to characterize size and morphology of micelles. Zeta potential was determined using a Nano-zeta potential analyzer. Sizeexclusion chromatography was used to separate different populations. Cytotoxic effect of micelles was evaluated on Hep2 cell line.Results: Covalent conjugation of spermidine to gangliosides produces a clear reduction of the electronegative z potential of micelle surface. DLS analysis shows no significant differences between both micelles, while TEM image shows a smaller size of Spermidine-GM1.These modified micelles load hydrophilic or hydrophobic antitumor drugs and conjugation does not affect the stability of micelles/drug in solution. Spermidine-GM1/Doxo micelles show faster drug release into cells as compared with GM1/Doxo micelles; however, no evidence can be found for the participation of the polyamine transport system in the uptake of modified micelles.Conclusion: While Spermidine-GM1 and GM1 micelles show similar physical properties, spermidine modified micelles are most efficient to release drugs, making this an interesting alternative to consider for drug delivery.Fil: Garro, Ariel Gustavo. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leonhard, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Heredia, Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Beltramo, Dante Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaBentham Science Publishers2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/179628Garro, Ariel Gustavo; Alasino, Roxana Valeria; Leonhard, Victoria; Heredia, Valeria; Beltramo, Dante Miguel; Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents; Bentham Science Publishers; Current Biotechnology; 6; 1; 3-2016; 50-572211-55012211-551XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/openurl/content.php?genre=article&issn=2211-5501&volume=6&issue=1&spage=50info:eu-repo/semantics/altIdentifier/doi/10.2174/2211550105666151228191616info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:41Zoai:ri.conicet.gov.ar:11336/179628instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:41.491CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents
title Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents
spellingShingle Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents
Garro, Ariel Gustavo
SPERMIDINE-GM1 MICELLES
POLYAMINES
DRUG TARGETING
CANCER THERAPY
title_short Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents
title_full Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents
title_fullStr Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents
title_full_unstemmed Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents
title_sort Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents
dc.creator.none.fl_str_mv Garro, Ariel Gustavo
Alasino, Roxana Valeria
Leonhard, Victoria
Heredia, Valeria
Beltramo, Dante Miguel
author Garro, Ariel Gustavo
author_facet Garro, Ariel Gustavo
Alasino, Roxana Valeria
Leonhard, Victoria
Heredia, Valeria
Beltramo, Dante Miguel
author_role author
author2 Alasino, Roxana Valeria
Leonhard, Victoria
Heredia, Valeria
Beltramo, Dante Miguel
author2_role author
author
author
author
dc.subject.none.fl_str_mv SPERMIDINE-GM1 MICELLES
POLYAMINES
DRUG TARGETING
CANCER THERAPY
topic SPERMIDINE-GM1 MICELLES
POLYAMINES
DRUG TARGETING
CANCER THERAPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Purpose: The ability of spermidine to increase the selectivity of anticancer agents has been studied extensively. In this research we report the combination of this polyamine and GM1 ganglioside micelles and characterize their behavior for drug delivery.Methods: Dynamic light scattering and electron microscopy were used to characterize size and morphology of micelles. Zeta potential was determined using a Nano-zeta potential analyzer. Sizeexclusion chromatography was used to separate different populations. Cytotoxic effect of micelles was evaluated on Hep2 cell line.Results: Covalent conjugation of spermidine to gangliosides produces a clear reduction of the electronegative z potential of micelle surface. DLS analysis shows no significant differences between both micelles, while TEM image shows a smaller size of Spermidine-GM1.These modified micelles load hydrophilic or hydrophobic antitumor drugs and conjugation does not affect the stability of micelles/drug in solution. Spermidine-GM1/Doxo micelles show faster drug release into cells as compared with GM1/Doxo micelles; however, no evidence can be found for the participation of the polyamine transport system in the uptake of modified micelles.Conclusion: While Spermidine-GM1 and GM1 micelles show similar physical properties, spermidine modified micelles are most efficient to release drugs, making this an interesting alternative to consider for drug delivery.
Fil: Garro, Ariel Gustavo. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leonhard, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Heredia, Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Beltramo, Dante Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
description Purpose: The ability of spermidine to increase the selectivity of anticancer agents has been studied extensively. In this research we report the combination of this polyamine and GM1 ganglioside micelles and characterize their behavior for drug delivery.Methods: Dynamic light scattering and electron microscopy were used to characterize size and morphology of micelles. Zeta potential was determined using a Nano-zeta potential analyzer. Sizeexclusion chromatography was used to separate different populations. Cytotoxic effect of micelles was evaluated on Hep2 cell line.Results: Covalent conjugation of spermidine to gangliosides produces a clear reduction of the electronegative z potential of micelle surface. DLS analysis shows no significant differences between both micelles, while TEM image shows a smaller size of Spermidine-GM1.These modified micelles load hydrophilic or hydrophobic antitumor drugs and conjugation does not affect the stability of micelles/drug in solution. Spermidine-GM1/Doxo micelles show faster drug release into cells as compared with GM1/Doxo micelles; however, no evidence can be found for the participation of the polyamine transport system in the uptake of modified micelles.Conclusion: While Spermidine-GM1 and GM1 micelles show similar physical properties, spermidine modified micelles are most efficient to release drugs, making this an interesting alternative to consider for drug delivery.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/179628
Garro, Ariel Gustavo; Alasino, Roxana Valeria; Leonhard, Victoria; Heredia, Valeria; Beltramo, Dante Miguel; Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents; Bentham Science Publishers; Current Biotechnology; 6; 1; 3-2016; 50-57
2211-5501
2211-551X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/179628
identifier_str_mv Garro, Ariel Gustavo; Alasino, Roxana Valeria; Leonhard, Victoria; Heredia, Valeria; Beltramo, Dante Miguel; Polyamines in the Surface of Lipid Micelles Improve the Cellular Uptake of Antitumoral Agents; Bentham Science Publishers; Current Biotechnology; 6; 1; 3-2016; 50-57
2211-5501
2211-551X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/openurl/content.php?genre=article&issn=2211-5501&volume=6&issue=1&spage=50
info:eu-repo/semantics/altIdentifier/doi/10.2174/2211550105666151228191616
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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