Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves

Autores
Murcia, Mauro Germán; Arias, Leonardo Agustín; Berli, Federico Javier; Muñoz, Flavio Andres; Fontana, Ariel Ramón; Piccoli, Patricia Noemí
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High-altitude grapevine cultivation (Vitis vinifera L.) offers a sustainable strategy for producing premium wines under climate change. However, the molecular mechanisms of UV-B acclimation in field-grown grapes remain unclear. This study examined the effects of contrasting UV-B exposure (exclusion vs. full exposure) on Malbec grape leaves and berries at different developmental stages in a high-altitude vineyard in Mendoza, Argentina (1350 m a.s.l.). Quantitative proteomics revealed tissue- and stage-specific responses to UV-B, with leaves at veraison being the most responsive. Only two proteins—omega-hydroxypalmitate O-feruloyl transferase and chalcone isomerase—were consistently upregulated across all tissues and stages. In silico analysis of their promoters suggested regulation by light, hormones, and stress signals. UV-B altered both primary and secondary metabolism. Photosynthesis-related proteins were reduced in veraison leaves but increased in pre-veraison berry skins. Stress-related proteins showed opposite trends across development in berries. Polyphenol biosynthesis and antioxidant capacity were enhanced in +UV-B treatment, with transketolase, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DHS), and chalcone isomerase emerging as key contributors to polyphenol accumulation. At veraison, UV-B induced lignin and flavonoid biosynthesis in leaves and promoted anthocyanin biosynthesis in berry skins, favoring the flavonoid 3′,5′-hydroxylase (F3′5′H) branch. UVR8 isoforms abundance correlated with anthocyanin-related responses, and a caffeoyl-CoA O-methyltransferase-2 isoform may mediate delphinidin methylation under UV-B stress. As berries matured, antioxidant defenses shifted from non-enzymatic to enzymatic mechanisms. This first proteomic study of high-altitude, field-grown grape berries provides new insights into UV-B acclimation and its potential to enhance grape and wine quality in a changing climate.
Fil: Murcia, Mauro Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Arias, Leonardo Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Muñoz, Flavio Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
PROTEOMICS
UV-B
GRAPEVINE
MALBEC
PHENOLIC COMPOUNDS
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275154
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leavesMurcia, Mauro GermánArias, Leonardo AgustínBerli, Federico JavierMuñoz, Flavio AndresFontana, Ariel RamónPiccoli, Patricia NoemíPROTEOMICSUV-BGRAPEVINEMALBECPHENOLIC COMPOUNDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1High-altitude grapevine cultivation (Vitis vinifera L.) offers a sustainable strategy for producing premium wines under climate change. However, the molecular mechanisms of UV-B acclimation in field-grown grapes remain unclear. This study examined the effects of contrasting UV-B exposure (exclusion vs. full exposure) on Malbec grape leaves and berries at different developmental stages in a high-altitude vineyard in Mendoza, Argentina (1350 m a.s.l.). Quantitative proteomics revealed tissue- and stage-specific responses to UV-B, with leaves at veraison being the most responsive. Only two proteins—omega-hydroxypalmitate O-feruloyl transferase and chalcone isomerase—were consistently upregulated across all tissues and stages. In silico analysis of their promoters suggested regulation by light, hormones, and stress signals. UV-B altered both primary and secondary metabolism. Photosynthesis-related proteins were reduced in veraison leaves but increased in pre-veraison berry skins. Stress-related proteins showed opposite trends across development in berries. Polyphenol biosynthesis and antioxidant capacity were enhanced in +UV-B treatment, with transketolase, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DHS), and chalcone isomerase emerging as key contributors to polyphenol accumulation. At veraison, UV-B induced lignin and flavonoid biosynthesis in leaves and promoted anthocyanin biosynthesis in berry skins, favoring the flavonoid 3′,5′-hydroxylase (F3′5′H) branch. UVR8 isoforms abundance correlated with anthocyanin-related responses, and a caffeoyl-CoA O-methyltransferase-2 isoform may mediate delphinidin methylation under UV-B stress. As berries matured, antioxidant defenses shifted from non-enzymatic to enzymatic mechanisms. This first proteomic study of high-altitude, field-grown grape berries provides new insights into UV-B acclimation and its potential to enhance grape and wine quality in a changing climate.Fil: Murcia, Mauro Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Arias, Leonardo Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Muñoz, Flavio Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaPergamon-Elsevier Science Ltd2025-07info:eu-repo/date/embargoEnd/2025-12-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275154Murcia, Mauro Germán; Arias, Leonardo Agustín; Berli, Federico Javier; Muñoz, Flavio Andres; Fontana, Ariel Ramón; et al.; Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 237; 7-2025; 1-480098-8472CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0098847225001157info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2025.106198info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:57:38Zoai:ri.conicet.gov.ar:11336/275154instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:57:38.916CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves
title Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves
spellingShingle Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves
Murcia, Mauro Germán
PROTEOMICS
UV-B
GRAPEVINE
MALBEC
PHENOLIC COMPOUNDS
title_short Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves
title_full Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves
title_fullStr Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves
title_full_unstemmed Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves
title_sort Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves
dc.creator.none.fl_str_mv Murcia, Mauro Germán
Arias, Leonardo Agustín
Berli, Federico Javier
Muñoz, Flavio Andres
Fontana, Ariel Ramón
Piccoli, Patricia Noemí
author Murcia, Mauro Germán
author_facet Murcia, Mauro Germán
Arias, Leonardo Agustín
Berli, Federico Javier
Muñoz, Flavio Andres
Fontana, Ariel Ramón
Piccoli, Patricia Noemí
author_role author
author2 Arias, Leonardo Agustín
Berli, Federico Javier
Muñoz, Flavio Andres
Fontana, Ariel Ramón
Piccoli, Patricia Noemí
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PROTEOMICS
UV-B
GRAPEVINE
MALBEC
PHENOLIC COMPOUNDS
topic PROTEOMICS
UV-B
GRAPEVINE
MALBEC
PHENOLIC COMPOUNDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv High-altitude grapevine cultivation (Vitis vinifera L.) offers a sustainable strategy for producing premium wines under climate change. However, the molecular mechanisms of UV-B acclimation in field-grown grapes remain unclear. This study examined the effects of contrasting UV-B exposure (exclusion vs. full exposure) on Malbec grape leaves and berries at different developmental stages in a high-altitude vineyard in Mendoza, Argentina (1350 m a.s.l.). Quantitative proteomics revealed tissue- and stage-specific responses to UV-B, with leaves at veraison being the most responsive. Only two proteins—omega-hydroxypalmitate O-feruloyl transferase and chalcone isomerase—were consistently upregulated across all tissues and stages. In silico analysis of their promoters suggested regulation by light, hormones, and stress signals. UV-B altered both primary and secondary metabolism. Photosynthesis-related proteins were reduced in veraison leaves but increased in pre-veraison berry skins. Stress-related proteins showed opposite trends across development in berries. Polyphenol biosynthesis and antioxidant capacity were enhanced in +UV-B treatment, with transketolase, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DHS), and chalcone isomerase emerging as key contributors to polyphenol accumulation. At veraison, UV-B induced lignin and flavonoid biosynthesis in leaves and promoted anthocyanin biosynthesis in berry skins, favoring the flavonoid 3′,5′-hydroxylase (F3′5′H) branch. UVR8 isoforms abundance correlated with anthocyanin-related responses, and a caffeoyl-CoA O-methyltransferase-2 isoform may mediate delphinidin methylation under UV-B stress. As berries matured, antioxidant defenses shifted from non-enzymatic to enzymatic mechanisms. This first proteomic study of high-altitude, field-grown grape berries provides new insights into UV-B acclimation and its potential to enhance grape and wine quality in a changing climate.
Fil: Murcia, Mauro Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Arias, Leonardo Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Muñoz, Flavio Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description High-altitude grapevine cultivation (Vitis vinifera L.) offers a sustainable strategy for producing premium wines under climate change. However, the molecular mechanisms of UV-B acclimation in field-grown grapes remain unclear. This study examined the effects of contrasting UV-B exposure (exclusion vs. full exposure) on Malbec grape leaves and berries at different developmental stages in a high-altitude vineyard in Mendoza, Argentina (1350 m a.s.l.). Quantitative proteomics revealed tissue- and stage-specific responses to UV-B, with leaves at veraison being the most responsive. Only two proteins—omega-hydroxypalmitate O-feruloyl transferase and chalcone isomerase—were consistently upregulated across all tissues and stages. In silico analysis of their promoters suggested regulation by light, hormones, and stress signals. UV-B altered both primary and secondary metabolism. Photosynthesis-related proteins were reduced in veraison leaves but increased in pre-veraison berry skins. Stress-related proteins showed opposite trends across development in berries. Polyphenol biosynthesis and antioxidant capacity were enhanced in +UV-B treatment, with transketolase, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DHS), and chalcone isomerase emerging as key contributors to polyphenol accumulation. At veraison, UV-B induced lignin and flavonoid biosynthesis in leaves and promoted anthocyanin biosynthesis in berry skins, favoring the flavonoid 3′,5′-hydroxylase (F3′5′H) branch. UVR8 isoforms abundance correlated with anthocyanin-related responses, and a caffeoyl-CoA O-methyltransferase-2 isoform may mediate delphinidin methylation under UV-B stress. As berries matured, antioxidant defenses shifted from non-enzymatic to enzymatic mechanisms. This first proteomic study of high-altitude, field-grown grape berries provides new insights into UV-B acclimation and its potential to enhance grape and wine quality in a changing climate.
publishDate 2025
dc.date.none.fl_str_mv 2025-07
info:eu-repo/date/embargoEnd/2025-12-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275154
Murcia, Mauro Germán; Arias, Leonardo Agustín; Berli, Federico Javier; Muñoz, Flavio Andres; Fontana, Ariel Ramón; et al.; Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 237; 7-2025; 1-48
0098-8472
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275154
identifier_str_mv Murcia, Mauro Germán; Arias, Leonardo Agustín; Berli, Federico Javier; Muñoz, Flavio Andres; Fontana, Ariel Ramón; et al.; Proteomic analysis reveals UV-B acclimation pathways in high-altitude Malbec berries and leaves; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 237; 7-2025; 1-48
0098-8472
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2025.106198
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
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eu_rights_str_mv embargoedAccess
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dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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