Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory

Autores
Schijman, Alejandro Gabriel; Alonso Padilla, Julio; Longhi, Silvia Andrea; Picado, Albert
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
There is no consensus on the diagnostic algorithms for many scenarios of Trypanosoma cruzi infection, which hinders the establishment of governmental guidelines in endemic and non-endemic countries. In the acute phase, parasitological methods are currently employed, and standardised surrogate molecular tests are being introduced to provide higher sensitivity and less operator-dependence. In the chronic phase, IgG-based serological assays are currently used, but if a single assay does not reach the required accuracy, PAHO/WHO recommends at least two immunological tests with different technical principles. Specific algorithms are applied to diagnose congenital infection, screen blood and organ donors or conduct epidemiological surveys. Detecting Chagas disease reactivation in immunosuppressed individuals is an area of increasing interest. Due to its neglect, enhancing access to diagnosis of patients at risk of suffering T. cruzi infection should be a priority at national and regional levels.
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Alonso Padilla, Julio. Universidad de Barcelona; España
Fil: Longhi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Picado, Albert. Foundation For Innovative New Diagnostics; Suiza
Materia
CHAGAS DISEASE
DIAGNOSIS
LOOP MEDIATED ISOTHERMAL AMPLIFICATION
POLYMERASE CHAIN REACTION
TRYPANOSOMA CRUZI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153129
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratorySchijman, Alejandro GabrielAlonso Padilla, JulioLonghi, Silvia AndreaPicado, AlbertCHAGAS DISEASEDIAGNOSISLOOP MEDIATED ISOTHERMAL AMPLIFICATIONPOLYMERASE CHAIN REACTIONTRYPANOSOMA CRUZIhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3There is no consensus on the diagnostic algorithms for many scenarios of Trypanosoma cruzi infection, which hinders the establishment of governmental guidelines in endemic and non-endemic countries. In the acute phase, parasitological methods are currently employed, and standardised surrogate molecular tests are being introduced to provide higher sensitivity and less operator-dependence. In the chronic phase, IgG-based serological assays are currently used, but if a single assay does not reach the required accuracy, PAHO/WHO recommends at least two immunological tests with different technical principles. Specific algorithms are applied to diagnose congenital infection, screen blood and organ donors or conduct epidemiological surveys. Detecting Chagas disease reactivation in immunosuppressed individuals is an area of increasing interest. Due to its neglect, enhancing access to diagnosis of patients at risk of suffering T. cruzi infection should be a priority at national and regional levels.Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Alonso Padilla, Julio. Universidad de Barcelona; EspañaFil: Longhi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Picado, Albert. Foundation For Innovative New Diagnostics; SuizaFundação Oswaldo Cruz2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153129Schijman, Alejandro Gabriel; Alonso Padilla, Julio; Longhi, Silvia Andrea; Picado, Albert; Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 116; 1; 7-2021; 1-130074-02761678-8060CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760200444info:eu-repo/semantics/altIdentifier/url/https://memorias.ioc.fiocruz.br/article/10806/0444-chagas-parasitological-serological-and-molecular-diagnosis-of-acute-and-chronic-chagas-disease-from-field-to-laboratoryinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:49:39Zoai:ri.conicet.gov.ar:11336/153129instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:49:40.12CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory
title Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory
spellingShingle Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory
Schijman, Alejandro Gabriel
CHAGAS DISEASE
DIAGNOSIS
LOOP MEDIATED ISOTHERMAL AMPLIFICATION
POLYMERASE CHAIN REACTION
TRYPANOSOMA CRUZI
title_short Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory
title_full Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory
title_fullStr Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory
title_full_unstemmed Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory
title_sort Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory
dc.creator.none.fl_str_mv Schijman, Alejandro Gabriel
Alonso Padilla, Julio
Longhi, Silvia Andrea
Picado, Albert
author Schijman, Alejandro Gabriel
author_facet Schijman, Alejandro Gabriel
Alonso Padilla, Julio
Longhi, Silvia Andrea
Picado, Albert
author_role author
author2 Alonso Padilla, Julio
Longhi, Silvia Andrea
Picado, Albert
author2_role author
author
author
dc.subject.none.fl_str_mv CHAGAS DISEASE
DIAGNOSIS
LOOP MEDIATED ISOTHERMAL AMPLIFICATION
POLYMERASE CHAIN REACTION
TRYPANOSOMA CRUZI
topic CHAGAS DISEASE
DIAGNOSIS
LOOP MEDIATED ISOTHERMAL AMPLIFICATION
POLYMERASE CHAIN REACTION
TRYPANOSOMA CRUZI
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv There is no consensus on the diagnostic algorithms for many scenarios of Trypanosoma cruzi infection, which hinders the establishment of governmental guidelines in endemic and non-endemic countries. In the acute phase, parasitological methods are currently employed, and standardised surrogate molecular tests are being introduced to provide higher sensitivity and less operator-dependence. In the chronic phase, IgG-based serological assays are currently used, but if a single assay does not reach the required accuracy, PAHO/WHO recommends at least two immunological tests with different technical principles. Specific algorithms are applied to diagnose congenital infection, screen blood and organ donors or conduct epidemiological surveys. Detecting Chagas disease reactivation in immunosuppressed individuals is an area of increasing interest. Due to its neglect, enhancing access to diagnosis of patients at risk of suffering T. cruzi infection should be a priority at national and regional levels.
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Alonso Padilla, Julio. Universidad de Barcelona; España
Fil: Longhi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Picado, Albert. Foundation For Innovative New Diagnostics; Suiza
description There is no consensus on the diagnostic algorithms for many scenarios of Trypanosoma cruzi infection, which hinders the establishment of governmental guidelines in endemic and non-endemic countries. In the acute phase, parasitological methods are currently employed, and standardised surrogate molecular tests are being introduced to provide higher sensitivity and less operator-dependence. In the chronic phase, IgG-based serological assays are currently used, but if a single assay does not reach the required accuracy, PAHO/WHO recommends at least two immunological tests with different technical principles. Specific algorithms are applied to diagnose congenital infection, screen blood and organ donors or conduct epidemiological surveys. Detecting Chagas disease reactivation in immunosuppressed individuals is an area of increasing interest. Due to its neglect, enhancing access to diagnosis of patients at risk of suffering T. cruzi infection should be a priority at national and regional levels.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153129
Schijman, Alejandro Gabriel; Alonso Padilla, Julio; Longhi, Silvia Andrea; Picado, Albert; Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 116; 1; 7-2021; 1-13
0074-0276
1678-8060
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153129
identifier_str_mv Schijman, Alejandro Gabriel; Alonso Padilla, Julio; Longhi, Silvia Andrea; Picado, Albert; Parasitological, serological and molecular diagnosis of acute and chronic chagas disease: From field to laboratory; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 116; 1; 7-2021; 1-13
0074-0276
1678-8060
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760200444
info:eu-repo/semantics/altIdentifier/url/https://memorias.ioc.fiocruz.br/article/10806/0444-chagas-parasitological-serological-and-molecular-diagnosis-of-acute-and-chronic-chagas-disease-from-field-to-laboratory
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083019213897728
score 13.22299

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