Steric Control of the Rate-Limiting Step of UDP-Galactopyranose Mutase

• Autores: Pierdominici Sottile, Gustavo; Cossio Pérez, Rodrigo; Da Fonseca, Isabel; Kizjakina, Karina; Tanner, John J.; Sobrado, Pablo
• Año de publicación: 2018
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Aspergillus fumigatus; UDP-galactopyranose mutase; Molecular dynamics; UGM
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Galactose is an abundant monosaccharide found exclusively in mammals as galactopyranose (Galp), the six-membered ring form of this sugar. In contrast, galactose appears in many pathogenic microorganisms as the five-membered ring form, galactofuranose (Galf). Galf biosynthesis begins with the conversion of UDP-Galp to UDP-Galf catalyzed by the flavoenzyme UDP-galactopyranose mutase (UGM). Because UGM is essential for the survival and proliferation of several pathogens, there is interest in understanding the catalytic mechanism to aid inhibitor development. Herein, we have used kinetic measurements and molecular dynamics simulations to explore the features of UGM that control the rate-limiting step (RLS). We show that UGM from the pathogenic fungus Aspergillus fumigatus also catalyzes the isomerization of UDP-arabinopyranose (UDP-Arap), which differs from UDP-Galp by lacking a -CH 2 -OH substituent at the C5 position of the hexose ring. Unexpectedly, the RLS changed from a chemical step for the natural substrate to product release with UDP-Arap. This result implicated residues that contact the -CH 2 -OH of UDP-Galp in controlling the mechanistic path. The mutation of one of these residues, Trp315, to Ala changed the RLS of the natural substrate to product release, similar to the wild-type enzyme with UDP-Arap. Molecular dynamics simulations suggest that steric complementarity in the Michaelis complex is responsible for this distinct behavior. These results provide new insight into the UGM mechanism and, more generally, how steric factors in the enzyme active site control the free energy barriers along the reaction path.
Fil: Pierdominici Sottile, Gustavo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cossio Pérez, Rodrigo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Da Fonseca, Isabel. Virginia Polytechnic Institute And State University; Estados Unidos
Fil: Kizjakina, Karina. Virginia Polytechnic Institute And State University; Estados Unidos
Fil: Tanner, John J.. University of Missouri; Estados Unidos
Fil: Sobrado, Pablo. Virginia Polytechnic Institute And State University; Estados Unidos