Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina

Autores
Willis Poratti, Graciana; Hedrich, Sabrina; Ñancucheo, Ivan; Johnson, D. Barrie; Donati, Edgardo Ruben
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The microbial diversity of sediment in the acidic Agua del Limón hot spring (located in the geothermal Caviahue-Copahue area, Argentina) was investigated using a combination of molecular and cultivation techniques, with particular emphasis on indigenous anaerobic prokaryotes. Bacteria involved in the iron (Acidithiobacillus ferrooxidans and Leptospirillum spp.) and sulfur (Acidithiobacillus spp., Thermotogales-like bacteria, Thiomonas sp., and Desulfurella sp.) cycles were identified in the clone library. Although no obvious sulfate-reducing bacteria were detected by culture-independent techniques, several isolates related to the mesophilic, sporeforming sulfate-reducer "Desulfobacillus acidavidus" strain CL4 were isolated on solid media incubated at both 30°C and 40°C. The 16S rRNA gene of another isolate showed 94% similarity to Desulfotomaculum thermobenzoicum. Enrichment cultur
Fil: Willis Poratti, Graciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Hedrich, Sabrina. Bangor University. School of Biological Sciences; Reino Unido
Fil: Ñancucheo, Ivan. Bangor University. School of Biological Sciences; Reino Unido
Fil: Johnson, D. Barrie. Bangor University. School of Biological Sciences; Reino Unido
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); Argentina
Materia
Sulfate Reducing
Acidophiles
Geothermal
Sediments
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4309
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4309
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, ArgentinaWillis Poratti, GracianaHedrich, SabrinaÑancucheo, IvanJohnson, D. BarrieDonati, Edgardo RubenSulfate ReducingAcidophilesGeothermalSedimentshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The microbial diversity of sediment in the acidic Agua del Limón hot spring (located in the geothermal Caviahue-Copahue area, Argentina) was investigated using a combination of molecular and cultivation techniques, with particular emphasis on indigenous anaerobic prokaryotes. Bacteria involved in the iron (Acidithiobacillus ferrooxidans and Leptospirillum spp.) and sulfur (Acidithiobacillus spp., Thermotogales-like bacteria, Thiomonas sp., and Desulfurella sp.) cycles were identified in the clone library. Although no obvious sulfate-reducing bacteria were detected by culture-independent techniques, several isolates related to the mesophilic, sporeforming sulfate-reducer "Desulfobacillus acidavidus" strain CL4 were isolated on solid media incubated at both 30°C and 40°C. The 16S rRNA gene of another isolate showed 94% similarity to Desulfotomaculum thermobenzoicum. Enrichment culturFil: Willis Poratti, Graciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Hedrich, Sabrina. Bangor University. School of Biological Sciences; Reino UnidoFil: Ñancucheo, Ivan. Bangor University. School of Biological Sciences; Reino UnidoFil: Johnson, D. Barrie. Bangor University. School of Biological Sciences; Reino UnidoFil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); ArgentinaTrans Tech Publications2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4309Willis Poratti, Graciana; Hedrich, Sabrina; Ñancucheo, Ivan; Johnson, D. Barrie; Donati, Edgardo Ruben; Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina; Trans Tech Publications; Advanced Materials Research; 825; 10-2013; 7-101662-8985enginfo:eu-repo/semantics/altIdentifier/url/http://www.scientific.net/AMR.825.7info:eu-repo/semantics/altIdentifier/doi/ doi:10.4028/www.scientific.net/AMR.825.7info:eu-repo/semantics/altIdentifier/issn/1662-8985info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:16Zoai:ri.conicet.gov.ar:11336/4309instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:16.688CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina
title Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina
spellingShingle Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina
Willis Poratti, Graciana
Sulfate Reducing
Acidophiles
Geothermal
Sediments
title_short Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina
title_full Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina
title_fullStr Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina
title_full_unstemmed Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina
title_sort Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina
dc.creator.none.fl_str_mv Willis Poratti, Graciana
Hedrich, Sabrina
Ñancucheo, Ivan
Johnson, D. Barrie
Donati, Edgardo Ruben
author Willis Poratti, Graciana
author_facet Willis Poratti, Graciana
Hedrich, Sabrina
Ñancucheo, Ivan
Johnson, D. Barrie
Donati, Edgardo Ruben
author_role author
author2 Hedrich, Sabrina
Ñancucheo, Ivan
Johnson, D. Barrie
Donati, Edgardo Ruben
author2_role author
author
author
author
dc.subject.none.fl_str_mv Sulfate Reducing
Acidophiles
Geothermal
Sediments
topic Sulfate Reducing
Acidophiles
Geothermal
Sediments
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The microbial diversity of sediment in the acidic Agua del Limón hot spring (located in the geothermal Caviahue-Copahue area, Argentina) was investigated using a combination of molecular and cultivation techniques, with particular emphasis on indigenous anaerobic prokaryotes. Bacteria involved in the iron (Acidithiobacillus ferrooxidans and Leptospirillum spp.) and sulfur (Acidithiobacillus spp., Thermotogales-like bacteria, Thiomonas sp., and Desulfurella sp.) cycles were identified in the clone library. Although no obvious sulfate-reducing bacteria were detected by culture-independent techniques, several isolates related to the mesophilic, sporeforming sulfate-reducer "Desulfobacillus acidavidus" strain CL4 were isolated on solid media incubated at both 30°C and 40°C. The 16S rRNA gene of another isolate showed 94% similarity to Desulfotomaculum thermobenzoicum. Enrichment cultur
Fil: Willis Poratti, Graciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Hedrich, Sabrina. Bangor University. School of Biological Sciences; Reino Unido
Fil: Ñancucheo, Ivan. Bangor University. School of Biological Sciences; Reino Unido
Fil: Johnson, D. Barrie. Bangor University. School of Biological Sciences; Reino Unido
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); Argentina
description The microbial diversity of sediment in the acidic Agua del Limón hot spring (located in the geothermal Caviahue-Copahue area, Argentina) was investigated using a combination of molecular and cultivation techniques, with particular emphasis on indigenous anaerobic prokaryotes. Bacteria involved in the iron (Acidithiobacillus ferrooxidans and Leptospirillum spp.) and sulfur (Acidithiobacillus spp., Thermotogales-like bacteria, Thiomonas sp., and Desulfurella sp.) cycles were identified in the clone library. Although no obvious sulfate-reducing bacteria were detected by culture-independent techniques, several isolates related to the mesophilic, sporeforming sulfate-reducer "Desulfobacillus acidavidus" strain CL4 were isolated on solid media incubated at both 30°C and 40°C. The 16S rRNA gene of another isolate showed 94% similarity to Desulfotomaculum thermobenzoicum. Enrichment cultur
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4309
Willis Poratti, Graciana; Hedrich, Sabrina; Ñancucheo, Ivan; Johnson, D. Barrie; Donati, Edgardo Ruben; Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina; Trans Tech Publications; Advanced Materials Research; 825; 10-2013; 7-10
1662-8985
url http://hdl.handle.net/11336/4309
identifier_str_mv Willis Poratti, Graciana; Hedrich, Sabrina; Ñancucheo, Ivan; Johnson, D. Barrie; Donati, Edgardo Ruben; Microbial diversity in acidic anaerobic sedimentsat the geothermal Caviahue-Copahue system, Argentina; Trans Tech Publications; Advanced Materials Research; 825; 10-2013; 7-10
1662-8985
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scientific.net/AMR.825.7
info:eu-repo/semantics/altIdentifier/doi/ doi:10.4028/www.scientific.net/AMR.825.7
info:eu-repo/semantics/altIdentifier/issn/1662-8985
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Trans Tech Publications
publisher.none.fl_str_mv Trans Tech Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269335098228736
score 13.13397

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