The bacterial protein Hfq: Much more than a mere RNA-binding factor

Autores
Sobrero, Patricio Martín; Valverde, Claudio Fabián
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Most of the sequenced bacterial genomes contain a gene encoding a protein known as Hfq that resembles the eukaryotic RNA-binding proteins of the LSm family. It was originally identified in Escherichia coli as a host factor required for replication of the Qβ RNA phage. In this review, we present a comprehensive summary of 40 years of investigation to learn that Hfq is an influential, though not essential, global regulator of gene expression in bacteria and that this feature is undoubtedly linked to Hfq's RNA-binding properties. This protein intervenes in different RNA transactions, notably the promotion of antisense interactions between messenger RNAs and small regulatory RNAs. Yet, several aspects of its molecular mechanism remain not understood. In addition, mechanistic studies have been exclusively carried out in enterobacterial models, highlighting the need to expand the research on Hfq function to other taxons. Upon reviewing the genetic, structural, biochemical, and biological aspects of this extraordinary protein, we discuss recent findings on interactions with macromolecules other than RNA suggesting a broader participation of Hfq in major steps in the flow of genetic information. We show that, although significant progress has been achieved to elucidate Hfq role at the molecular level, many open questions remain.
Fil: Sobrero, Patricio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Materia
GLOBAL REGULATOR
HFQ
NUCLEIC ACID BINDING PROTEIN
SM-PROTEIN
SRNAMRNA INTERACTIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195339
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling The bacterial protein Hfq: Much more than a mere RNA-binding factorSobrero, Patricio MartínValverde, Claudio FabiánGLOBAL REGULATORHFQNUCLEIC ACID BINDING PROTEINSM-PROTEINSRNAMRNA INTERACTIONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Most of the sequenced bacterial genomes contain a gene encoding a protein known as Hfq that resembles the eukaryotic RNA-binding proteins of the LSm family. It was originally identified in Escherichia coli as a host factor required for replication of the Qβ RNA phage. In this review, we present a comprehensive summary of 40 years of investigation to learn that Hfq is an influential, though not essential, global regulator of gene expression in bacteria and that this feature is undoubtedly linked to Hfq's RNA-binding properties. This protein intervenes in different RNA transactions, notably the promotion of antisense interactions between messenger RNAs and small regulatory RNAs. Yet, several aspects of its molecular mechanism remain not understood. In addition, mechanistic studies have been exclusively carried out in enterobacterial models, highlighting the need to expand the research on Hfq function to other taxons. Upon reviewing the genetic, structural, biochemical, and biological aspects of this extraordinary protein, we discuss recent findings on interactions with macromolecules other than RNA suggesting a broader participation of Hfq in major steps in the flow of genetic information. We show that, although significant progress has been achieved to elucidate Hfq role at the molecular level, many open questions remain.Fil: Sobrero, Patricio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaTaylor & Francis2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195339Sobrero, Patricio Martín; Valverde, Claudio Fabián; The bacterial protein Hfq: Much more than a mere RNA-binding factor; Taylor & Francis; Critical Reviews In Microbiology; 38; 4; 3-2012; 276-2991040-841XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/1040841X.2012.664540info:eu-repo/semantics/altIdentifier/doi/10.3109/1040841X.2012.664540info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T10:00:38Zoai:ri.conicet.gov.ar:11336/195339instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 10:00:38.945CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The bacterial protein Hfq: Much more than a mere RNA-binding factor
title The bacterial protein Hfq: Much more than a mere RNA-binding factor
spellingShingle The bacterial protein Hfq: Much more than a mere RNA-binding factor
Sobrero, Patricio Martín
GLOBAL REGULATOR
HFQ
NUCLEIC ACID BINDING PROTEIN
SM-PROTEIN
SRNAMRNA INTERACTIONS
title_short The bacterial protein Hfq: Much more than a mere RNA-binding factor
title_full The bacterial protein Hfq: Much more than a mere RNA-binding factor
title_fullStr The bacterial protein Hfq: Much more than a mere RNA-binding factor
title_full_unstemmed The bacterial protein Hfq: Much more than a mere RNA-binding factor
title_sort The bacterial protein Hfq: Much more than a mere RNA-binding factor
dc.creator.none.fl_str_mv Sobrero, Patricio Martín
Valverde, Claudio Fabián
author Sobrero, Patricio Martín
author_facet Sobrero, Patricio Martín
Valverde, Claudio Fabián
author_role author
author2 Valverde, Claudio Fabián
author2_role author
dc.subject.none.fl_str_mv GLOBAL REGULATOR
HFQ
NUCLEIC ACID BINDING PROTEIN
SM-PROTEIN
SRNAMRNA INTERACTIONS
topic GLOBAL REGULATOR
HFQ
NUCLEIC ACID BINDING PROTEIN
SM-PROTEIN
SRNAMRNA INTERACTIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Most of the sequenced bacterial genomes contain a gene encoding a protein known as Hfq that resembles the eukaryotic RNA-binding proteins of the LSm family. It was originally identified in Escherichia coli as a host factor required for replication of the Qβ RNA phage. In this review, we present a comprehensive summary of 40 years of investigation to learn that Hfq is an influential, though not essential, global regulator of gene expression in bacteria and that this feature is undoubtedly linked to Hfq's RNA-binding properties. This protein intervenes in different RNA transactions, notably the promotion of antisense interactions between messenger RNAs and small regulatory RNAs. Yet, several aspects of its molecular mechanism remain not understood. In addition, mechanistic studies have been exclusively carried out in enterobacterial models, highlighting the need to expand the research on Hfq function to other taxons. Upon reviewing the genetic, structural, biochemical, and biological aspects of this extraordinary protein, we discuss recent findings on interactions with macromolecules other than RNA suggesting a broader participation of Hfq in major steps in the flow of genetic information. We show that, although significant progress has been achieved to elucidate Hfq role at the molecular level, many open questions remain.
Fil: Sobrero, Patricio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
description Most of the sequenced bacterial genomes contain a gene encoding a protein known as Hfq that resembles the eukaryotic RNA-binding proteins of the LSm family. It was originally identified in Escherichia coli as a host factor required for replication of the Qβ RNA phage. In this review, we present a comprehensive summary of 40 years of investigation to learn that Hfq is an influential, though not essential, global regulator of gene expression in bacteria and that this feature is undoubtedly linked to Hfq's RNA-binding properties. This protein intervenes in different RNA transactions, notably the promotion of antisense interactions between messenger RNAs and small regulatory RNAs. Yet, several aspects of its molecular mechanism remain not understood. In addition, mechanistic studies have been exclusively carried out in enterobacterial models, highlighting the need to expand the research on Hfq function to other taxons. Upon reviewing the genetic, structural, biochemical, and biological aspects of this extraordinary protein, we discuss recent findings on interactions with macromolecules other than RNA suggesting a broader participation of Hfq in major steps in the flow of genetic information. We show that, although significant progress has been achieved to elucidate Hfq role at the molecular level, many open questions remain.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195339
Sobrero, Patricio Martín; Valverde, Claudio Fabián; The bacterial protein Hfq: Much more than a mere RNA-binding factor; Taylor & Francis; Critical Reviews In Microbiology; 38; 4; 3-2012; 276-299
1040-841X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195339
identifier_str_mv Sobrero, Patricio Martín; Valverde, Claudio Fabián; The bacterial protein Hfq: Much more than a mere RNA-binding factor; Taylor & Francis; Critical Reviews In Microbiology; 38; 4; 3-2012; 276-299
1040-841X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/1040841X.2012.664540
info:eu-repo/semantics/altIdentifier/doi/10.3109/1040841X.2012.664540
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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