Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata

Autores
Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Wuilloud, Rodolfo German; Clament, María E.; Castro Vazquez, Alfredo Juan
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effects of TBT on mortality and reproduction were studied in this invasive snail. The nominal fifty percent lethal concentration (LC50) was 9 Dg TBT/L, after 28 days. The nominal no observed effect on lethality concentration? (NOECL) was 6 Dg TBT/L after the same period. Male-female couples and females that had been group-mated but were isolated from males during the experiment (isolated females) were exposed (28 d) to either 0 or 6 Dg/L of TBT (nominal NOECL-exposed). Copulation and oviposition frequencies, egg clutch mass and percent egg fertility were recorded. Gonads (both sexes) and the seminal receptacle (females) were studied histologically at the end of the experiment. A significant decrease in copulation frequency was observed in mated-exposed females. Exposure also decreased oviposition frequency of mated exposed and isolated-exposed females, but only the latter reached significance. No differences in either egg clutch mass or percent fertility were observed at first oviposition but both parameters were drastically reduced in subsequent egg clutches of exposed females. No histological alterations were observed in gonads of TBT-exposed animals; however, sperm storage in the seminal receptacle was drastically decreased in exposed females. Imposex but no oviductal obstruction was observed in all exposed females. It is concluded that TBT induces reproductive failure in P. canaliculata by decreasing copulation frequency and by severely affecting sperm storage by the female.
Fil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Cientifico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Cientifico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Wuilloud, Rodolfo German. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Clament, María E.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Castro Vazquez, Alfredo Juan. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Cientifico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Materia
Endocrine Disruptors
Imposex
Fertilization-Spermicides
Apple Snails
Fertilization
Spermicides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10096
Acceder
id CONICETDig_71e17c674fc4822c56bf6ad7956c2505
oai_identifier_str oai:ri.conicet.gov.ar:11336/10096
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculataGiraud Billoud, Maximiliano GermanVega, Israel AníbalWuilloud, Rodolfo GermanClament, María E.Castro Vazquez, Alfredo JuanEndocrine DisruptorsImposexFertilization-SpermicidesApple SnailsFertilizationSpermicideshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effects of TBT on mortality and reproduction were studied in this invasive snail. The nominal fifty percent lethal concentration (LC50) was 9 Dg TBT/L, after 28 days. The nominal no observed effect on lethality concentration? (NOECL) was 6 Dg TBT/L after the same period. Male-female couples and females that had been group-mated but were isolated from males during the experiment (isolated females) were exposed (28 d) to either 0 or 6 Dg/L of TBT (nominal NOECL-exposed). Copulation and oviposition frequencies, egg clutch mass and percent egg fertility were recorded. Gonads (both sexes) and the seminal receptacle (females) were studied histologically at the end of the experiment. A significant decrease in copulation frequency was observed in mated-exposed females. Exposure also decreased oviposition frequency of mated exposed and isolated-exposed females, but only the latter reached significance. No differences in either egg clutch mass or percent fertility were observed at first oviposition but both parameters were drastically reduced in subsequent egg clutches of exposed females. No histological alterations were observed in gonads of TBT-exposed animals; however, sperm storage in the seminal receptacle was drastically decreased in exposed females. Imposex but no oviductal obstruction was observed in all exposed females. It is concluded that TBT induces reproductive failure in P. canaliculata by decreasing copulation frequency and by severely affecting sperm storage by the female.Fil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Cientifico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; ArgentinaFil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Cientifico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Wuilloud, Rodolfo German. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Clament, María E.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Castro Vazquez, Alfredo Juan. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Cientifico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaSetac Press2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10096Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Wuilloud, Rodolfo German; Clament, María E.; Castro Vazquez, Alfredo Juan; Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata; Setac Press; Environmental Toxicology And Chemistry; 32; 6-2013; 2365-23710730-7268enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/etc.2310/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/etc.2310info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:01Zoai:ri.conicet.gov.ar:11336/10096instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:02.16CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata
title Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata
spellingShingle Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata
Giraud Billoud, Maximiliano German
Endocrine Disruptors
Imposex
Fertilization-Spermicides
Apple Snails
Fertilization
Spermicides
title_short Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata
title_full Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata
title_fullStr Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata
title_full_unstemmed Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata
title_sort Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata
dc.creator.none.fl_str_mv Giraud Billoud, Maximiliano German
Vega, Israel Aníbal
Wuilloud, Rodolfo German
Clament, María E.
Castro Vazquez, Alfredo Juan
author Giraud Billoud, Maximiliano German
author_facet Giraud Billoud, Maximiliano German
Vega, Israel Aníbal
Wuilloud, Rodolfo German
Clament, María E.
Castro Vazquez, Alfredo Juan
author_role author
author2 Vega, Israel Aníbal
Wuilloud, Rodolfo German
Clament, María E.
Castro Vazquez, Alfredo Juan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Endocrine Disruptors
Imposex
Fertilization-Spermicides
Apple Snails
Fertilization
Spermicides
topic Endocrine Disruptors
Imposex
Fertilization-Spermicides
Apple Snails
Fertilization
Spermicides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effects of TBT on mortality and reproduction were studied in this invasive snail. The nominal fifty percent lethal concentration (LC50) was 9 Dg TBT/L, after 28 days. The nominal no observed effect on lethality concentration? (NOECL) was 6 Dg TBT/L after the same period. Male-female couples and females that had been group-mated but were isolated from males during the experiment (isolated females) were exposed (28 d) to either 0 or 6 Dg/L of TBT (nominal NOECL-exposed). Copulation and oviposition frequencies, egg clutch mass and percent egg fertility were recorded. Gonads (both sexes) and the seminal receptacle (females) were studied histologically at the end of the experiment. A significant decrease in copulation frequency was observed in mated-exposed females. Exposure also decreased oviposition frequency of mated exposed and isolated-exposed females, but only the latter reached significance. No differences in either egg clutch mass or percent fertility were observed at first oviposition but both parameters were drastically reduced in subsequent egg clutches of exposed females. No histological alterations were observed in gonads of TBT-exposed animals; however, sperm storage in the seminal receptacle was drastically decreased in exposed females. Imposex but no oviductal obstruction was observed in all exposed females. It is concluded that TBT induces reproductive failure in P. canaliculata by decreasing copulation frequency and by severely affecting sperm storage by the female.
Fil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Cientifico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Cientifico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Wuilloud, Rodolfo German. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Clament, María E.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Castro Vazquez, Alfredo Juan. Consejo Nacional de Investigaciones Cientficas y Técnicas. Centro Cientifico Tecnológico Mendoza. Instituto Histología y Embriología de Mendoza "Dr. M. Burgos"; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
description The effects of TBT on mortality and reproduction were studied in this invasive snail. The nominal fifty percent lethal concentration (LC50) was 9 Dg TBT/L, after 28 days. The nominal no observed effect on lethality concentration? (NOECL) was 6 Dg TBT/L after the same period. Male-female couples and females that had been group-mated but were isolated from males during the experiment (isolated females) were exposed (28 d) to either 0 or 6 Dg/L of TBT (nominal NOECL-exposed). Copulation and oviposition frequencies, egg clutch mass and percent egg fertility were recorded. Gonads (both sexes) and the seminal receptacle (females) were studied histologically at the end of the experiment. A significant decrease in copulation frequency was observed in mated-exposed females. Exposure also decreased oviposition frequency of mated exposed and isolated-exposed females, but only the latter reached significance. No differences in either egg clutch mass or percent fertility were observed at first oviposition but both parameters were drastically reduced in subsequent egg clutches of exposed females. No histological alterations were observed in gonads of TBT-exposed animals; however, sperm storage in the seminal receptacle was drastically decreased in exposed females. Imposex but no oviductal obstruction was observed in all exposed females. It is concluded that TBT induces reproductive failure in P. canaliculata by decreasing copulation frequency and by severely affecting sperm storage by the female.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10096
Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Wuilloud, Rodolfo German; Clament, María E.; Castro Vazquez, Alfredo Juan; Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata; Setac Press; Environmental Toxicology And Chemistry; 32; 6-2013; 2365-2371
0730-7268
url http://hdl.handle.net/11336/10096
identifier_str_mv Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Wuilloud, Rodolfo German; Clament, María E.; Castro Vazquez, Alfredo Juan; Imposex and novel mechanisms of reproductive failure induced by tributyltin (TBT) in the freshwater snail Pomacea canaliculata; Setac Press; Environmental Toxicology And Chemistry; 32; 6-2013; 2365-2371
0730-7268
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/etc.2310/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.2310
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Setac Press
publisher.none.fl_str_mv Setac Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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