Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae

Autores
da Silva, Rodrigo Alves; Quintela, Eliane Dias; Mascarin, Gabriel Moura; Pedrini, Nicolás; Lião, Luciano Moraes; Ferri, Pedro Henrique
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Eggs, nymphs (1st-5th instar) and adults of Tibraca limbativentris were challenged by conidial suspensions of its major fungal pathogen Metarhizium anisopliae in order to assess their susceptibility. The role of chemical defensive compounds from exocrine secretions produced by both nymphs and adults were examined for their participation on M. anisopliae infection. Although insect susceptibility to M. anisopliae followed a dose-dependent manner, adults followed by older nymphs displayed the highest resistance. Eggs were highly susceptible showing >96% fungal infection. Crude extracts isolated from metathoracic scent gland and dorsal abdominal glands of adults and nymphs, respectively, showed fungistatic effects by impairing spore germination, vegetative growth and sporulation. Gas chromatography-mass spectrometry analysis of these extracts revealed that the major components were short-chain hydrocarbons (C10-13) and unsaturated aldehydes. In vitro tests with the corresponding synthetic standards indicated compounds with greater antifungal activity including (E)-2-hexenal, (E)-2-octenal, and (E)-2-decenal, with the latter being the most deleterious to fungal fitness. We demonstrated that differential susceptibility of the rice stalk stink bug to M. anisopliae infection is age-specific and partly mediated by fungistatic properties of aldehydes, which are produced by scent glands of both nymphs and adults.
Fil: da Silva, Rodrigo Alves. Instituto Federal de Brasília; Brasil
Fil: Quintela, Eliane Dias. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Mascarin, Gabriel Moura. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Lião, Luciano Moraes. Universidade Federal de Goiás; Brasil
Fil: Ferri, Pedro Henrique. Universidade Federal de Goiás; Brasil
Materia
ALLELOCHEMICALS
CHEMICAL ECOLOGY
CLAVICIPITACEAE
ORYZA SATIVA
TIBRACA LIMBATIVENTRIS
VIRULENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100088
Acceder
id CONICETDig_71986cd142e7c7e820993d1241d91b87
oai_identifier_str oai:ri.conicet.gov.ar:11336/100088
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliaeda Silva, Rodrigo AlvesQuintela, Eliane DiasMascarin, Gabriel MouraPedrini, NicolásLião, Luciano MoraesFerri, Pedro HenriqueALLELOCHEMICALSCHEMICAL ECOLOGYCLAVICIPITACEAEORYZA SATIVATIBRACA LIMBATIVENTRISVIRULENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Eggs, nymphs (1st-5th instar) and adults of Tibraca limbativentris were challenged by conidial suspensions of its major fungal pathogen Metarhizium anisopliae in order to assess their susceptibility. The role of chemical defensive compounds from exocrine secretions produced by both nymphs and adults were examined for their participation on M. anisopliae infection. Although insect susceptibility to M. anisopliae followed a dose-dependent manner, adults followed by older nymphs displayed the highest resistance. Eggs were highly susceptible showing >96% fungal infection. Crude extracts isolated from metathoracic scent gland and dorsal abdominal glands of adults and nymphs, respectively, showed fungistatic effects by impairing spore germination, vegetative growth and sporulation. Gas chromatography-mass spectrometry analysis of these extracts revealed that the major components were short-chain hydrocarbons (C10-13) and unsaturated aldehydes. In vitro tests with the corresponding synthetic standards indicated compounds with greater antifungal activity including (E)-2-hexenal, (E)-2-octenal, and (E)-2-decenal, with the latter being the most deleterious to fungal fitness. We demonstrated that differential susceptibility of the rice stalk stink bug to M. anisopliae infection is age-specific and partly mediated by fungistatic properties of aldehydes, which are produced by scent glands of both nymphs and adults.Fil: da Silva, Rodrigo Alves. Instituto Federal de Brasília; BrasilFil: Quintela, Eliane Dias. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Mascarin, Gabriel Moura. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Lião, Luciano Moraes. Universidade Federal de Goiás; BrasilFil: Ferri, Pedro Henrique. Universidade Federal de Goiás; BrasilAcademic Press Inc Elsevier Science2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100088da Silva, Rodrigo Alves; Quintela, Eliane Dias; Mascarin, Gabriel Moura; Pedrini, Nicolás; Lião, Luciano Moraes; et al.; Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae; Academic Press Inc Elsevier Science; Journal of Invertebrate Pathology; 127; 5-2015; 93-1000022-2011CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022201115000646info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2015.03.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:46Zoai:ri.conicet.gov.ar:11336/100088instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:46.647CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae
title Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae
spellingShingle Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae
da Silva, Rodrigo Alves
ALLELOCHEMICALS
CHEMICAL ECOLOGY
CLAVICIPITACEAE
ORYZA SATIVA
TIBRACA LIMBATIVENTRIS
VIRULENCE
title_short Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae
title_full Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae
title_fullStr Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae
title_full_unstemmed Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae
title_sort Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae
dc.creator.none.fl_str_mv da Silva, Rodrigo Alves
Quintela, Eliane Dias
Mascarin, Gabriel Moura
Pedrini, Nicolás
Lião, Luciano Moraes
Ferri, Pedro Henrique
author da Silva, Rodrigo Alves
author_facet da Silva, Rodrigo Alves
Quintela, Eliane Dias
Mascarin, Gabriel Moura
Pedrini, Nicolás
Lião, Luciano Moraes
Ferri, Pedro Henrique
author_role author
author2 Quintela, Eliane Dias
Mascarin, Gabriel Moura
Pedrini, Nicolás
Lião, Luciano Moraes
Ferri, Pedro Henrique
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ALLELOCHEMICALS
CHEMICAL ECOLOGY
CLAVICIPITACEAE
ORYZA SATIVA
TIBRACA LIMBATIVENTRIS
VIRULENCE
topic ALLELOCHEMICALS
CHEMICAL ECOLOGY
CLAVICIPITACEAE
ORYZA SATIVA
TIBRACA LIMBATIVENTRIS
VIRULENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Eggs, nymphs (1st-5th instar) and adults of Tibraca limbativentris were challenged by conidial suspensions of its major fungal pathogen Metarhizium anisopliae in order to assess their susceptibility. The role of chemical defensive compounds from exocrine secretions produced by both nymphs and adults were examined for their participation on M. anisopliae infection. Although insect susceptibility to M. anisopliae followed a dose-dependent manner, adults followed by older nymphs displayed the highest resistance. Eggs were highly susceptible showing >96% fungal infection. Crude extracts isolated from metathoracic scent gland and dorsal abdominal glands of adults and nymphs, respectively, showed fungistatic effects by impairing spore germination, vegetative growth and sporulation. Gas chromatography-mass spectrometry analysis of these extracts revealed that the major components were short-chain hydrocarbons (C10-13) and unsaturated aldehydes. In vitro tests with the corresponding synthetic standards indicated compounds with greater antifungal activity including (E)-2-hexenal, (E)-2-octenal, and (E)-2-decenal, with the latter being the most deleterious to fungal fitness. We demonstrated that differential susceptibility of the rice stalk stink bug to M. anisopliae infection is age-specific and partly mediated by fungistatic properties of aldehydes, which are produced by scent glands of both nymphs and adults.
Fil: da Silva, Rodrigo Alves. Instituto Federal de Brasília; Brasil
Fil: Quintela, Eliane Dias. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Mascarin, Gabriel Moura. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Lião, Luciano Moraes. Universidade Federal de Goiás; Brasil
Fil: Ferri, Pedro Henrique. Universidade Federal de Goiás; Brasil
description Eggs, nymphs (1st-5th instar) and adults of Tibraca limbativentris were challenged by conidial suspensions of its major fungal pathogen Metarhizium anisopliae in order to assess their susceptibility. The role of chemical defensive compounds from exocrine secretions produced by both nymphs and adults were examined for their participation on M. anisopliae infection. Although insect susceptibility to M. anisopliae followed a dose-dependent manner, adults followed by older nymphs displayed the highest resistance. Eggs were highly susceptible showing >96% fungal infection. Crude extracts isolated from metathoracic scent gland and dorsal abdominal glands of adults and nymphs, respectively, showed fungistatic effects by impairing spore germination, vegetative growth and sporulation. Gas chromatography-mass spectrometry analysis of these extracts revealed that the major components were short-chain hydrocarbons (C10-13) and unsaturated aldehydes. In vitro tests with the corresponding synthetic standards indicated compounds with greater antifungal activity including (E)-2-hexenal, (E)-2-octenal, and (E)-2-decenal, with the latter being the most deleterious to fungal fitness. We demonstrated that differential susceptibility of the rice stalk stink bug to M. anisopliae infection is age-specific and partly mediated by fungistatic properties of aldehydes, which are produced by scent glands of both nymphs and adults.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100088
da Silva, Rodrigo Alves; Quintela, Eliane Dias; Mascarin, Gabriel Moura; Pedrini, Nicolás; Lião, Luciano Moraes; et al.; Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae; Academic Press Inc Elsevier Science; Journal of Invertebrate Pathology; 127; 5-2015; 93-100
0022-2011
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100088
identifier_str_mv da Silva, Rodrigo Alves; Quintela, Eliane Dias; Mascarin, Gabriel Moura; Pedrini, Nicolás; Lião, Luciano Moraes; et al.; Unveiling chemical defense in the rice stalk stink bug against the entomopathogenic fungus Metarhizium anisopliae; Academic Press Inc Elsevier Science; Journal of Invertebrate Pathology; 127; 5-2015; 93-100
0022-2011
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022201115000646
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2015.03.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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