Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis

Autores
Forrellad, Marina Andrea; McNeil, Michael; Santangelo, María de la Paz; Blanco, Federico Carlos; Garcia, Elizabeth Andrea; Klepp, Laura Ines; Huff, Jason; Niederweis, Michael; Jackson, Mary; Bigi, Fabiana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tuberculosis is one of the leading causes of mortality throughout the world. Mycobacterium tuberculosis, the causative agent of human tuberculosis, has developed several strategies involving proteins and other compounds known collectively as virulence factors to subvert human host defences and invade the human host. The Mce proteins are among these virulence-related proteins and are encoded by the mce1, mce2, mce3 and mce4 operons in the genome of M. tuberculosis. It has been proposed that these operons encode ABC-like lipid transporters; however, the nature of their substrates has only been revealed in the case of the Mce4 proteins. Here we found that the knockout of the mce1 operon alters the lipid profile of M. tuberculosis H37Rv and the uptake of palmitic acid. Thin layer chromatography and liquid chromatography-mass spectrometry analysis showed that the mce1 mutant accumulates more mycolic acids than the wild type and complemented strains. Interestingly, this accumulation of mycolic acid is exacerbate when bacteria are cultured in the presence of palmitic acid or arachidonic acid, growth conditions that may mimic the intracellular environment. These results suggest that the mce1 operon may serve as a mycolic acid re-importer.
Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: McNeil, Michael. State University of Colorado - Fort Collins; Estados Unidos
Fil: Santangelo, María de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Huff, Jason. University of Alabama at Birmingahm; Estados Unidos
Fil: Niederweis, Michael. University of Alabama at Birmingahm; Estados Unidos
Fil: Jackson, Mary. State University of Colorado - Fort Collins; Estados Unidos
Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Materia
LIPIDS
MYCOBACTERIUM TUBERCULOSIS
MYCOLIC ACID
MCE OPERON
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33236
Acceder
id CONICETDig_7133d15a243968035316a6aca14ce857
oai_identifier_str oai:ri.conicet.gov.ar:11336/33236
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosisForrellad, Marina AndreaMcNeil, MichaelSantangelo, María de la PazBlanco, Federico CarlosGarcia, Elizabeth AndreaKlepp, Laura InesHuff, JasonNiederweis, MichaelJackson, MaryBigi, FabianaLIPIDSMYCOBACTERIUM TUBERCULOSISMYCOLIC ACIDMCE OPERONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Tuberculosis is one of the leading causes of mortality throughout the world. Mycobacterium tuberculosis, the causative agent of human tuberculosis, has developed several strategies involving proteins and other compounds known collectively as virulence factors to subvert human host defences and invade the human host. The Mce proteins are among these virulence-related proteins and are encoded by the mce1, mce2, mce3 and mce4 operons in the genome of M. tuberculosis. It has been proposed that these operons encode ABC-like lipid transporters; however, the nature of their substrates has only been revealed in the case of the Mce4 proteins. Here we found that the knockout of the mce1 operon alters the lipid profile of M. tuberculosis H37Rv and the uptake of palmitic acid. Thin layer chromatography and liquid chromatography-mass spectrometry analysis showed that the mce1 mutant accumulates more mycolic acids than the wild type and complemented strains. Interestingly, this accumulation of mycolic acid is exacerbate when bacteria are cultured in the presence of palmitic acid or arachidonic acid, growth conditions that may mimic the intracellular environment. These results suggest that the mce1 operon may serve as a mycolic acid re-importer.Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: McNeil, Michael. State University of Colorado - Fort Collins; Estados UnidosFil: Santangelo, María de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Huff, Jason. University of Alabama at Birmingahm; Estados UnidosFil: Niederweis, Michael. University of Alabama at Birmingahm; Estados UnidosFil: Jackson, Mary. State University of Colorado - Fort Collins; Estados UnidosFil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaElsevier2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33236Bigi, Fabiana; Jackson, Mary; Niederweis, Michael; McNeil, Michael; Garcia, Elizabeth Andrea; Huff, Jason; et al.; Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis; Elsevier; Tuberculosis (Edinb); 94; 2; 3-2014; 170-1771472-9792CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1472979213002230info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tube.2013.12.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:31Zoai:ri.conicet.gov.ar:11336/33236instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:31.255CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis
title Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis
spellingShingle Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis
Forrellad, Marina Andrea
LIPIDS
MYCOBACTERIUM TUBERCULOSIS
MYCOLIC ACID
MCE OPERON
title_short Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis
title_full Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis
title_fullStr Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis
title_full_unstemmed Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis
title_sort Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis
dc.creator.none.fl_str_mv Forrellad, Marina Andrea
McNeil, Michael
Santangelo, María de la Paz
Blanco, Federico Carlos
Garcia, Elizabeth Andrea
Klepp, Laura Ines
Huff, Jason
Niederweis, Michael
Jackson, Mary
Bigi, Fabiana
author Forrellad, Marina Andrea
author_facet Forrellad, Marina Andrea
McNeil, Michael
Santangelo, María de la Paz
Blanco, Federico Carlos
Garcia, Elizabeth Andrea
Klepp, Laura Ines
Huff, Jason
Niederweis, Michael
Jackson, Mary
Bigi, Fabiana
author_role author
author2 McNeil, Michael
Santangelo, María de la Paz
Blanco, Federico Carlos
Garcia, Elizabeth Andrea
Klepp, Laura Ines
Huff, Jason
Niederweis, Michael
Jackson, Mary
Bigi, Fabiana
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LIPIDS
MYCOBACTERIUM TUBERCULOSIS
MYCOLIC ACID
MCE OPERON
topic LIPIDS
MYCOBACTERIUM TUBERCULOSIS
MYCOLIC ACID
MCE OPERON
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Tuberculosis is one of the leading causes of mortality throughout the world. Mycobacterium tuberculosis, the causative agent of human tuberculosis, has developed several strategies involving proteins and other compounds known collectively as virulence factors to subvert human host defences and invade the human host. The Mce proteins are among these virulence-related proteins and are encoded by the mce1, mce2, mce3 and mce4 operons in the genome of M. tuberculosis. It has been proposed that these operons encode ABC-like lipid transporters; however, the nature of their substrates has only been revealed in the case of the Mce4 proteins. Here we found that the knockout of the mce1 operon alters the lipid profile of M. tuberculosis H37Rv and the uptake of palmitic acid. Thin layer chromatography and liquid chromatography-mass spectrometry analysis showed that the mce1 mutant accumulates more mycolic acids than the wild type and complemented strains. Interestingly, this accumulation of mycolic acid is exacerbate when bacteria are cultured in the presence of palmitic acid or arachidonic acid, growth conditions that may mimic the intracellular environment. These results suggest that the mce1 operon may serve as a mycolic acid re-importer.
Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: McNeil, Michael. State University of Colorado - Fort Collins; Estados Unidos
Fil: Santangelo, María de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Huff, Jason. University of Alabama at Birmingahm; Estados Unidos
Fil: Niederweis, Michael. University of Alabama at Birmingahm; Estados Unidos
Fil: Jackson, Mary. State University of Colorado - Fort Collins; Estados Unidos
Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
description Tuberculosis is one of the leading causes of mortality throughout the world. Mycobacterium tuberculosis, the causative agent of human tuberculosis, has developed several strategies involving proteins and other compounds known collectively as virulence factors to subvert human host defences and invade the human host. The Mce proteins are among these virulence-related proteins and are encoded by the mce1, mce2, mce3 and mce4 operons in the genome of M. tuberculosis. It has been proposed that these operons encode ABC-like lipid transporters; however, the nature of their substrates has only been revealed in the case of the Mce4 proteins. Here we found that the knockout of the mce1 operon alters the lipid profile of M. tuberculosis H37Rv and the uptake of palmitic acid. Thin layer chromatography and liquid chromatography-mass spectrometry analysis showed that the mce1 mutant accumulates more mycolic acids than the wild type and complemented strains. Interestingly, this accumulation of mycolic acid is exacerbate when bacteria are cultured in the presence of palmitic acid or arachidonic acid, growth conditions that may mimic the intracellular environment. These results suggest that the mce1 operon may serve as a mycolic acid re-importer.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33236
Bigi, Fabiana; Jackson, Mary; Niederweis, Michael; McNeil, Michael; Garcia, Elizabeth Andrea; Huff, Jason; et al.; Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis; Elsevier; Tuberculosis (Edinb); 94; 2; 3-2014; 170-177
1472-9792
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33236
identifier_str_mv Bigi, Fabiana; Jackson, Mary; Niederweis, Michael; McNeil, Michael; Garcia, Elizabeth Andrea; Huff, Jason; et al.; Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis; Elsevier; Tuberculosis (Edinb); 94; 2; 3-2014; 170-177
1472-9792
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1472979213002230
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tube.2013.12.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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