Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment

Autores
Behnke, Gevan D.; Kim, Nakian; Riggins, Change; Zabaloy, Maria Celina; Rodriguez Zas, Sandra; Villamil, Maria Bonita
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Much of the global nitrous oxide emissions are derived from agricultural management driving microbial N transformations. Crop rotation, no-till, and cover cropping are feasible agronomic strategies to prevent N losses to the environment, though their effect on soil microbial N cycling at the field scale remains relatively unknown. Our goal was to determine the effect of crop rotation (continuous corn, CCC; and continuous soybean, SSS), tillage (no-till, NT; and chisel tillage, T), and cover crops (cover crop mixture, CC; and no cover crop, NCC) on the quantification of functional genes related to the N cycle from different times throughout the growing season. The study was conducted during the growing season of the cash crops following a first season of cover crops introduced after 23 years of management. Using quantitative polymerase chain reaction (qPCR) techniques, we quantified nifH (N2 fixation), amoA (nitrification) and nirK, nirS, and nosZ (denitrification). Our results show that CCC increased nitrous oxide emissions by 44% compared to SSS and reduced soil pH by nearly 1 unit. The reduction in soil pH, coupled with an increase in fertilizer-derived ammonium, caused ammonia-oxidizing bacteria (AOB) and nirK copy numbers to increase. The SSS rotation showed opposite results. Chisel tillage was found to increase all N cycle gene counts compared to no-till. The cover crop mixture of cereal rye (Secale cereale L.) and hairy vetch (Vicia villosa Roth) significantly reduced soil nitrate levels, though they did increase nitrous oxide emissions, possibly due to the inclusion of a legume in the cover crop mixture. In corn-dominated systems, more fertilizer N is required to maintain yields, leading to increased nitrous oxide emissions through bacterial nitrification and nirK denitrification caused by high ammonium concentrations and acidic soil conditions.
Fil: Behnke, Gevan D.. University of Illinois at Urbana; Estados Unidos
Fil: Kim, Nakian. University of Illinois at Urbana; Estados Unidos
Fil: Riggins, Change. University of Illinois at Urbana; Estados Unidos
Fil: Zabaloy, Maria Celina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Rodriguez Zas, Sandra. University of Illinois at Urbana; Estados Unidos
Fil: Villamil, Maria Bonita. University of Illinois at Urbana; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
ASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable Development
Salt Lake City
Estados Unidos
American Society of Agronomy
Crop Science Society of America
Soil Science Society of America
Materia
COVER CROPS
SOYBEAN
CORN
TILLAGE
N CYCLE GENES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/255643
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/255643
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural ExperimentBehnke, Gevan D.Kim, NakianRiggins, ChangeZabaloy, Maria CelinaRodriguez Zas, SandraVillamil, Maria BonitaCOVER CROPSSOYBEANCORNTILLAGEN CYCLE GENEShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Much of the global nitrous oxide emissions are derived from agricultural management driving microbial N transformations. Crop rotation, no-till, and cover cropping are feasible agronomic strategies to prevent N losses to the environment, though their effect on soil microbial N cycling at the field scale remains relatively unknown. Our goal was to determine the effect of crop rotation (continuous corn, CCC; and continuous soybean, SSS), tillage (no-till, NT; and chisel tillage, T), and cover crops (cover crop mixture, CC; and no cover crop, NCC) on the quantification of functional genes related to the N cycle from different times throughout the growing season. The study was conducted during the growing season of the cash crops following a first season of cover crops introduced after 23 years of management. Using quantitative polymerase chain reaction (qPCR) techniques, we quantified nifH (N2 fixation), amoA (nitrification) and nirK, nirS, and nosZ (denitrification). Our results show that CCC increased nitrous oxide emissions by 44% compared to SSS and reduced soil pH by nearly 1 unit. The reduction in soil pH, coupled with an increase in fertilizer-derived ammonium, caused ammonia-oxidizing bacteria (AOB) and nirK copy numbers to increase. The SSS rotation showed opposite results. Chisel tillage was found to increase all N cycle gene counts compared to no-till. The cover crop mixture of cereal rye (Secale cereale L.) and hairy vetch (Vicia villosa Roth) significantly reduced soil nitrate levels, though they did increase nitrous oxide emissions, possibly due to the inclusion of a legume in the cover crop mixture. In corn-dominated systems, more fertilizer N is required to maintain yields, leading to increased nitrous oxide emissions through bacterial nitrification and nirK denitrification caused by high ammonium concentrations and acidic soil conditions.Fil: Behnke, Gevan D.. University of Illinois at Urbana; Estados UnidosFil: Kim, Nakian. University of Illinois at Urbana; Estados UnidosFil: Riggins, Change. University of Illinois at Urbana; Estados UnidosFil: Zabaloy, Maria Celina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Rodriguez Zas, Sandra. University of Illinois at Urbana; Estados UnidosFil: Villamil, Maria Bonita. University of Illinois at Urbana; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable DevelopmentSalt Lake CityEstados UnidosAmerican Society of AgronomyCrop Science Society of AmericaSoil Science Society of AmericaAmerican Society of Agronomy2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/255643Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment; ASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable Development; Salt Lake City; Estados Unidos; 2021; 1-4CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://scisoc.confex.com/scisoc/2021am/meetingapp.cgi/Paper/135613Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:11Zoai:ri.conicet.gov.ar:11336/255643instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:12.191CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment
title Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment
spellingShingle Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment
Behnke, Gevan D.
COVER CROPS
SOYBEAN
CORN
TILLAGE
N CYCLE GENES
title_short Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment
title_full Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment
title_fullStr Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment
title_full_unstemmed Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment
title_sort Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment
dc.creator.none.fl_str_mv Behnke, Gevan D.
Kim, Nakian
Riggins, Change
Zabaloy, Maria Celina
Rodriguez Zas, Sandra
Villamil, Maria Bonita
author Behnke, Gevan D.
author_facet Behnke, Gevan D.
Kim, Nakian
Riggins, Change
Zabaloy, Maria Celina
Rodriguez Zas, Sandra
Villamil, Maria Bonita
author_role author
author2 Kim, Nakian
Riggins, Change
Zabaloy, Maria Celina
Rodriguez Zas, Sandra
Villamil, Maria Bonita
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv COVER CROPS
SOYBEAN
CORN
TILLAGE
N CYCLE GENES
topic COVER CROPS
SOYBEAN
CORN
TILLAGE
N CYCLE GENES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Much of the global nitrous oxide emissions are derived from agricultural management driving microbial N transformations. Crop rotation, no-till, and cover cropping are feasible agronomic strategies to prevent N losses to the environment, though their effect on soil microbial N cycling at the field scale remains relatively unknown. Our goal was to determine the effect of crop rotation (continuous corn, CCC; and continuous soybean, SSS), tillage (no-till, NT; and chisel tillage, T), and cover crops (cover crop mixture, CC; and no cover crop, NCC) on the quantification of functional genes related to the N cycle from different times throughout the growing season. The study was conducted during the growing season of the cash crops following a first season of cover crops introduced after 23 years of management. Using quantitative polymerase chain reaction (qPCR) techniques, we quantified nifH (N2 fixation), amoA (nitrification) and nirK, nirS, and nosZ (denitrification). Our results show that CCC increased nitrous oxide emissions by 44% compared to SSS and reduced soil pH by nearly 1 unit. The reduction in soil pH, coupled with an increase in fertilizer-derived ammonium, caused ammonia-oxidizing bacteria (AOB) and nirK copy numbers to increase. The SSS rotation showed opposite results. Chisel tillage was found to increase all N cycle gene counts compared to no-till. The cover crop mixture of cereal rye (Secale cereale L.) and hairy vetch (Vicia villosa Roth) significantly reduced soil nitrate levels, though they did increase nitrous oxide emissions, possibly due to the inclusion of a legume in the cover crop mixture. In corn-dominated systems, more fertilizer N is required to maintain yields, leading to increased nitrous oxide emissions through bacterial nitrification and nirK denitrification caused by high ammonium concentrations and acidic soil conditions.
Fil: Behnke, Gevan D.. University of Illinois at Urbana; Estados Unidos
Fil: Kim, Nakian. University of Illinois at Urbana; Estados Unidos
Fil: Riggins, Change. University of Illinois at Urbana; Estados Unidos
Fil: Zabaloy, Maria Celina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Rodriguez Zas, Sandra. University of Illinois at Urbana; Estados Unidos
Fil: Villamil, Maria Bonita. University of Illinois at Urbana; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
ASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable Development
Salt Lake City
Estados Unidos
American Society of Agronomy
Crop Science Society of America
Soil Science Society of America
description Much of the global nitrous oxide emissions are derived from agricultural management driving microbial N transformations. Crop rotation, no-till, and cover cropping are feasible agronomic strategies to prevent N losses to the environment, though their effect on soil microbial N cycling at the field scale remains relatively unknown. Our goal was to determine the effect of crop rotation (continuous corn, CCC; and continuous soybean, SSS), tillage (no-till, NT; and chisel tillage, T), and cover crops (cover crop mixture, CC; and no cover crop, NCC) on the quantification of functional genes related to the N cycle from different times throughout the growing season. The study was conducted during the growing season of the cash crops following a first season of cover crops introduced after 23 years of management. Using quantitative polymerase chain reaction (qPCR) techniques, we quantified nifH (N2 fixation), amoA (nitrification) and nirK, nirS, and nosZ (denitrification). Our results show that CCC increased nitrous oxide emissions by 44% compared to SSS and reduced soil pH by nearly 1 unit. The reduction in soil pH, coupled with an increase in fertilizer-derived ammonium, caused ammonia-oxidizing bacteria (AOB) and nirK copy numbers to increase. The SSS rotation showed opposite results. Chisel tillage was found to increase all N cycle gene counts compared to no-till. The cover crop mixture of cereal rye (Secale cereale L.) and hairy vetch (Vicia villosa Roth) significantly reduced soil nitrate levels, though they did increase nitrous oxide emissions, possibly due to the inclusion of a legume in the cover crop mixture. In corn-dominated systems, more fertilizer N is required to maintain yields, leading to increased nitrous oxide emissions through bacterial nitrification and nirK denitrification caused by high ammonium concentrations and acidic soil conditions.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/255643
Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment; ASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable Development; Salt Lake City; Estados Unidos; 2021; 1-4
CONICET Digital
CONICET
url http://hdl.handle.net/11336/255643
identifier_str_mv Longitudinal Study of the Microbial Basis of N2O Emissions within a Long-Term Agricultural Experiment; ASA, CSSA, SSSA International Annual Meeting: A Creative Economy for Sustainable Development; Salt Lake City; Estados Unidos; 2021; 1-4
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://scisoc.confex.com/scisoc/2021am/meetingapp.cgi/Paper/135613
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv American Society of Agronomy
publisher.none.fl_str_mv American Society of Agronomy
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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