Relevance of oncobiome in breast cancer evolution in an Argentine cohort

Autores
Dandeu, Leonardo Néstor Rubén; Lachovsky, Joel; Sidlik, Sofia; Marenco, Pablo; Orschanski, Daniela; Aguilera, Pablo Nicolas; Vázquez, Martín; Carballo, María del Pilar; Fernández, Elmer; Penas Steinhardt, Alberto; Chasseing, Norma Alejandra; Labovsky, Vivian
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Breast cancer is the leading cause of cancer deaths in women worldwide, with about 20,000 cases annually in Argentina. While age, diet, and genetics are known risk factors, most breast cancer cases have unknown causes, necessitating the discovery of new risk factors. The aim of this study was the analysis of the prognostic relevance of the oncobiome in Argentinean breast cancer patients. Sequencing of the V4 region 16S rRNA gene was performed on 34 primary breast tumor samples, using bioinformatic and statistical analyses to identify bacteria and hypothetical pathways. Each sample presented a unique microbial profile, with Proteobacteria being the most abundant phylum. Tumors >2 cm showed greater alpha diversity with increased nucleotide biosynthesis. Moreover, progesterone-receptor tumors showed differences in beta diversity, being progesterone receptor-positive tumors that had the highest expression of Acinetobacter and Moraxella. In disease progression, the phylum Chloroflexi was prevalent in tumors of surviving patients. Acinetobacter and Cloacibacterium genera were significantly higher in patients without events and those without metastasis. We found that nucleotide and cell-structure biosynthesis, and lipid metabolism pathways were enriched in tumors with poor progression, whereas amino-acid degradation was increased in tumors of surviving patients. This finding is an indication that tumor cells are taking advantage of this effect of the microbiome during tumor progression. We conclude that oncobiome is dysbiotic in these patients, with distinct patterns in those with poor progression. Suggesting a link between the oncobiome and cancer progression, paving the way for new therapies to improve patient quality of life and survival.
Fil: Dandeu, Leonardo Néstor Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lachovsky, Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sidlik, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Marenco, Pablo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina
Fil: Orschanski, Daniela. Fundación Para El Progreso de la Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aguilera, Pablo Nicolas. Universidad Nacional de Moreno. Departamento de Cs. Aplicadas y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vázquez, Martín. Heritas S.a (heritas S.a);
Fil: Carballo, María del Pilar. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina
Fil: Fernández, Elmer. Fundación Para El Progreso de la Medicina; Argentina
Fil: Penas Steinhardt, Alberto. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Genomica Computacional.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chasseing, Norma Alejandra. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Labovsky, Vivian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
TUMOR MICROENVIRONMENT
ONCOBIOME
BREAST CANCER
PROGRESION DISEASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280350
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/280350
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Relevance of oncobiome in breast cancer evolution in an Argentine cohortDandeu, Leonardo Néstor RubénLachovsky, JoelSidlik, SofiaMarenco, PabloOrschanski, DanielaAguilera, Pablo NicolasVázquez, MartínCarballo, María del PilarFernández, ElmerPenas Steinhardt, AlbertoChasseing, Norma AlejandraLabovsky, VivianTUMOR MICROENVIRONMENTONCOBIOMEBREAST CANCERPROGRESION DISEASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Breast cancer is the leading cause of cancer deaths in women worldwide, with about 20,000 cases annually in Argentina. While age, diet, and genetics are known risk factors, most breast cancer cases have unknown causes, necessitating the discovery of new risk factors. The aim of this study was the analysis of the prognostic relevance of the oncobiome in Argentinean breast cancer patients. Sequencing of the V4 region 16S rRNA gene was performed on 34 primary breast tumor samples, using bioinformatic and statistical analyses to identify bacteria and hypothetical pathways. Each sample presented a unique microbial profile, with Proteobacteria being the most abundant phylum. Tumors >2 cm showed greater alpha diversity with increased nucleotide biosynthesis. Moreover, progesterone-receptor tumors showed differences in beta diversity, being progesterone receptor-positive tumors that had the highest expression of Acinetobacter and Moraxella. In disease progression, the phylum Chloroflexi was prevalent in tumors of surviving patients. Acinetobacter and Cloacibacterium genera were significantly higher in patients without events and those without metastasis. We found that nucleotide and cell-structure biosynthesis, and lipid metabolism pathways were enriched in tumors with poor progression, whereas amino-acid degradation was increased in tumors of surviving patients. This finding is an indication that tumor cells are taking advantage of this effect of the microbiome during tumor progression. We conclude that oncobiome is dysbiotic in these patients, with distinct patterns in those with poor progression. Suggesting a link between the oncobiome and cancer progression, paving the way for new therapies to improve patient quality of life and survival.Fil: Dandeu, Leonardo Néstor Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lachovsky, Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Sidlik, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Marenco, Pablo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; ArgentinaFil: Orschanski, Daniela. Fundación Para El Progreso de la Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aguilera, Pablo Nicolas. Universidad Nacional de Moreno. Departamento de Cs. Aplicadas y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vázquez, Martín. Heritas S.a (heritas S.a);Fil: Carballo, María del Pilar. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; ArgentinaFil: Fernández, Elmer. Fundación Para El Progreso de la Medicina; ArgentinaFil: Penas Steinhardt, Alberto. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Genomica Computacional.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chasseing, Norma Alejandra. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Labovsky, Vivian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaAmerican Society for Microbiology2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280350Dandeu, Leonardo Néstor Rubén; Lachovsky, Joel; Sidlik, Sofia; Marenco, Pablo; Orschanski, Daniela; et al.; Relevance of oncobiome in breast cancer evolution in an Argentine cohort; American Society for Microbiology; mSphere; 10; 3; 1-2025; 1-152379-5042CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/full/10.1128/msphere.00597-24info:eu-repo/semantics/altIdentifier/doi/10.1128/msphere.00597-24info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:04:38Zoai:ri.conicet.gov.ar:11336/280350instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:04:38.425CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Relevance of oncobiome in breast cancer evolution in an Argentine cohort
title Relevance of oncobiome in breast cancer evolution in an Argentine cohort
spellingShingle Relevance of oncobiome in breast cancer evolution in an Argentine cohort
Dandeu, Leonardo Néstor Rubén
TUMOR MICROENVIRONMENT
ONCOBIOME
BREAST CANCER
PROGRESION DISEASE
title_short Relevance of oncobiome in breast cancer evolution in an Argentine cohort
title_full Relevance of oncobiome in breast cancer evolution in an Argentine cohort
title_fullStr Relevance of oncobiome in breast cancer evolution in an Argentine cohort
title_full_unstemmed Relevance of oncobiome in breast cancer evolution in an Argentine cohort
title_sort Relevance of oncobiome in breast cancer evolution in an Argentine cohort
dc.creator.none.fl_str_mv Dandeu, Leonardo Néstor Rubén
Lachovsky, Joel
Sidlik, Sofia
Marenco, Pablo
Orschanski, Daniela
Aguilera, Pablo Nicolas
Vázquez, Martín
Carballo, María del Pilar
Fernández, Elmer
Penas Steinhardt, Alberto
Chasseing, Norma Alejandra
Labovsky, Vivian
author Dandeu, Leonardo Néstor Rubén
author_facet Dandeu, Leonardo Néstor Rubén
Lachovsky, Joel
Sidlik, Sofia
Marenco, Pablo
Orschanski, Daniela
Aguilera, Pablo Nicolas
Vázquez, Martín
Carballo, María del Pilar
Fernández, Elmer
Penas Steinhardt, Alberto
Chasseing, Norma Alejandra
Labovsky, Vivian
author_role author
author2 Lachovsky, Joel
Sidlik, Sofia
Marenco, Pablo
Orschanski, Daniela
Aguilera, Pablo Nicolas
Vázquez, Martín
Carballo, María del Pilar
Fernández, Elmer
Penas Steinhardt, Alberto
Chasseing, Norma Alejandra
Labovsky, Vivian
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TUMOR MICROENVIRONMENT
ONCOBIOME
BREAST CANCER
PROGRESION DISEASE
topic TUMOR MICROENVIRONMENT
ONCOBIOME
BREAST CANCER
PROGRESION DISEASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Breast cancer is the leading cause of cancer deaths in women worldwide, with about 20,000 cases annually in Argentina. While age, diet, and genetics are known risk factors, most breast cancer cases have unknown causes, necessitating the discovery of new risk factors. The aim of this study was the analysis of the prognostic relevance of the oncobiome in Argentinean breast cancer patients. Sequencing of the V4 region 16S rRNA gene was performed on 34 primary breast tumor samples, using bioinformatic and statistical analyses to identify bacteria and hypothetical pathways. Each sample presented a unique microbial profile, with Proteobacteria being the most abundant phylum. Tumors >2 cm showed greater alpha diversity with increased nucleotide biosynthesis. Moreover, progesterone-receptor tumors showed differences in beta diversity, being progesterone receptor-positive tumors that had the highest expression of Acinetobacter and Moraxella. In disease progression, the phylum Chloroflexi was prevalent in tumors of surviving patients. Acinetobacter and Cloacibacterium genera were significantly higher in patients without events and those without metastasis. We found that nucleotide and cell-structure biosynthesis, and lipid metabolism pathways were enriched in tumors with poor progression, whereas amino-acid degradation was increased in tumors of surviving patients. This finding is an indication that tumor cells are taking advantage of this effect of the microbiome during tumor progression. We conclude that oncobiome is dysbiotic in these patients, with distinct patterns in those with poor progression. Suggesting a link between the oncobiome and cancer progression, paving the way for new therapies to improve patient quality of life and survival.
Fil: Dandeu, Leonardo Néstor Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lachovsky, Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sidlik, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Marenco, Pablo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina
Fil: Orschanski, Daniela. Fundación Para El Progreso de la Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aguilera, Pablo Nicolas. Universidad Nacional de Moreno. Departamento de Cs. Aplicadas y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vázquez, Martín. Heritas S.a (heritas S.a);
Fil: Carballo, María del Pilar. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina
Fil: Fernández, Elmer. Fundación Para El Progreso de la Medicina; Argentina
Fil: Penas Steinhardt, Alberto. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Genomica Computacional.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chasseing, Norma Alejandra. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Labovsky, Vivian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Breast cancer is the leading cause of cancer deaths in women worldwide, with about 20,000 cases annually in Argentina. While age, diet, and genetics are known risk factors, most breast cancer cases have unknown causes, necessitating the discovery of new risk factors. The aim of this study was the analysis of the prognostic relevance of the oncobiome in Argentinean breast cancer patients. Sequencing of the V4 region 16S rRNA gene was performed on 34 primary breast tumor samples, using bioinformatic and statistical analyses to identify bacteria and hypothetical pathways. Each sample presented a unique microbial profile, with Proteobacteria being the most abundant phylum. Tumors >2 cm showed greater alpha diversity with increased nucleotide biosynthesis. Moreover, progesterone-receptor tumors showed differences in beta diversity, being progesterone receptor-positive tumors that had the highest expression of Acinetobacter and Moraxella. In disease progression, the phylum Chloroflexi was prevalent in tumors of surviving patients. Acinetobacter and Cloacibacterium genera were significantly higher in patients without events and those without metastasis. We found that nucleotide and cell-structure biosynthesis, and lipid metabolism pathways were enriched in tumors with poor progression, whereas amino-acid degradation was increased in tumors of surviving patients. This finding is an indication that tumor cells are taking advantage of this effect of the microbiome during tumor progression. We conclude that oncobiome is dysbiotic in these patients, with distinct patterns in those with poor progression. Suggesting a link between the oncobiome and cancer progression, paving the way for new therapies to improve patient quality of life and survival.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280350
Dandeu, Leonardo Néstor Rubén; Lachovsky, Joel; Sidlik, Sofia; Marenco, Pablo; Orschanski, Daniela; et al.; Relevance of oncobiome in breast cancer evolution in an Argentine cohort; American Society for Microbiology; mSphere; 10; 3; 1-2025; 1-15
2379-5042
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280350
identifier_str_mv Dandeu, Leonardo Néstor Rubén; Lachovsky, Joel; Sidlik, Sofia; Marenco, Pablo; Orschanski, Daniela; et al.; Relevance of oncobiome in breast cancer evolution in an Argentine cohort; American Society for Microbiology; mSphere; 10; 3; 1-2025; 1-15
2379-5042
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/full/10.1128/msphere.00597-24
info:eu-repo/semantics/altIdentifier/doi/10.1128/msphere.00597-24
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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