Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress

Autores
Menezes Sobreira, Alana Cecília; Maia, Yuri; Rebouças, Deborah Moura; Costa, José Hélio; Oliveira Otoch, Maria de Lourdes; Nogueira de Oliveira, Luciana Maia; Orellano, Elena Graciela; Fernandes de Melo, Dirce
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Global climatic changes as high temperatures and low precipitation contribute to increase cultivated areas affected by high salt soil content. Soil salinity is well known to reduce the ability of plants to take up water and this quickly causes reduction in their growth rate. V-ATPase (EC 3.6.3.14) and V-PPase (EC 3.6.1.1) hydrolytic and proton transport activities, and gene expression were evaluated in hypocotyls of 3-, 5-, 7-day-old Vigna unguiculata (L.) Walp cv. Vita 3 germinated in 100 mM NaCl in order to highlight their differential regulation and activity modulation under salt stress. Semi-quantitative RT-PCR revealed that both genes were up-regulated by salt stress in all salt exposition times studied. Up-regulation was correlated with the increase in protein content at 5 and 7-day-old seedlings. Co-expression between A and E V-ATPase subunits was also observed. The hydrolytic and proton transport activities showed that these enzymes presented a differential modulation of their activities in the presence of 100 mM NaCl. These results suggest that V-ATPase and V-PPase activities are modulated by salt stress and a multi-step regulation is exerted in order to re-establish homeostasis
Fil: Menezes Sobreira, Alana Cecília. Universidade Estadual Do Ceara; Brasil. Universidade Federal Do Ceara; Brasil
Fil: Maia, Yuri. Universidade Federal Do Ceara; Brasil
Fil: Rebouças, Deborah Moura. Universidade Federal Do Ceara; Brasil
Fil: Costa, José Hélio. Universidade Federal Do Ceara; Brasil
Fil: Oliveira Otoch, Maria de Lourdes. Universidade Estadual Do Ceara; Brasil
Fil: Nogueira de Oliveira, Luciana Maia. Universidade Federal Rural Pernambuco; Brasil
Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Fernandes de Melo, Dirce. Universidade Federal do Ceará; Brasil
Materia
Cowpea
Proton Pump
Salinity Stress
Vacuolar Membrane
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29630
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stressMenezes Sobreira, Alana CecíliaMaia, YuriRebouças, Deborah MouraCosta, José HélioOliveira Otoch, Maria de LourdesNogueira de Oliveira, Luciana MaiaOrellano, Elena GracielaFernandes de Melo, DirceCowpeaProton PumpSalinity StressVacuolar Membranehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Global climatic changes as high temperatures and low precipitation contribute to increase cultivated areas affected by high salt soil content. Soil salinity is well known to reduce the ability of plants to take up water and this quickly causes reduction in their growth rate. V-ATPase (EC 3.6.3.14) and V-PPase (EC 3.6.1.1) hydrolytic and proton transport activities, and gene expression were evaluated in hypocotyls of 3-, 5-, 7-day-old Vigna unguiculata (L.) Walp cv. Vita 3 germinated in 100 mM NaCl in order to highlight their differential regulation and activity modulation under salt stress. Semi-quantitative RT-PCR revealed that both genes were up-regulated by salt stress in all salt exposition times studied. Up-regulation was correlated with the increase in protein content at 5 and 7-day-old seedlings. Co-expression between A and E V-ATPase subunits was also observed. The hydrolytic and proton transport activities showed that these enzymes presented a differential modulation of their activities in the presence of 100 mM NaCl. These results suggest that V-ATPase and V-PPase activities are modulated by salt stress and a multi-step regulation is exerted in order to re-establish homeostasisFil: Menezes Sobreira, Alana Cecília. Universidade Estadual Do Ceara; Brasil. Universidade Federal Do Ceara; BrasilFil: Maia, Yuri. Universidade Federal Do Ceara; BrasilFil: Rebouças, Deborah Moura. Universidade Federal Do Ceara; BrasilFil: Costa, José Hélio. Universidade Federal Do Ceara; BrasilFil: Oliveira Otoch, Maria de Lourdes. Universidade Estadual Do Ceara; BrasilFil: Nogueira de Oliveira, Luciana Maia. Universidade Federal Rural Pernambuco; BrasilFil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Fernandes de Melo, Dirce. Universidade Federal do Ceará; BrasilSpringer2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29630Menezes Sobreira, Alana Cecília; Maia, Yuri; Rebouças, Deborah Moura; Costa, José Hélio; Oliveira Otoch, Maria de Lourdes; et al.; Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress; Springer; Theoretical and Experimental Plant Physiology; 26; 5-2014; 167-1752197-0025CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s40626-014-0012-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs40626-014-0012-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:04:33Zoai:ri.conicet.gov.ar:11336/29630instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:04:33.336CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress
title Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress
spellingShingle Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress
Menezes Sobreira, Alana Cecília
Cowpea
Proton Pump
Salinity Stress
Vacuolar Membrane
title_short Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress
title_full Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress
title_fullStr Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress
title_full_unstemmed Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress
title_sort Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress
dc.creator.none.fl_str_mv Menezes Sobreira, Alana Cecília
Maia, Yuri
Rebouças, Deborah Moura
Costa, José Hélio
Oliveira Otoch, Maria de Lourdes
Nogueira de Oliveira, Luciana Maia
Orellano, Elena Graciela
Fernandes de Melo, Dirce
author Menezes Sobreira, Alana Cecília
author_facet Menezes Sobreira, Alana Cecília
Maia, Yuri
Rebouças, Deborah Moura
Costa, José Hélio
Oliveira Otoch, Maria de Lourdes
Nogueira de Oliveira, Luciana Maia
Orellano, Elena Graciela
Fernandes de Melo, Dirce
author_role author
author2 Maia, Yuri
Rebouças, Deborah Moura
Costa, José Hélio
Oliveira Otoch, Maria de Lourdes
Nogueira de Oliveira, Luciana Maia
Orellano, Elena Graciela
Fernandes de Melo, Dirce
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cowpea
Proton Pump
Salinity Stress
Vacuolar Membrane
topic Cowpea
Proton Pump
Salinity Stress
Vacuolar Membrane
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Global climatic changes as high temperatures and low precipitation contribute to increase cultivated areas affected by high salt soil content. Soil salinity is well known to reduce the ability of plants to take up water and this quickly causes reduction in their growth rate. V-ATPase (EC 3.6.3.14) and V-PPase (EC 3.6.1.1) hydrolytic and proton transport activities, and gene expression were evaluated in hypocotyls of 3-, 5-, 7-day-old Vigna unguiculata (L.) Walp cv. Vita 3 germinated in 100 mM NaCl in order to highlight their differential regulation and activity modulation under salt stress. Semi-quantitative RT-PCR revealed that both genes were up-regulated by salt stress in all salt exposition times studied. Up-regulation was correlated with the increase in protein content at 5 and 7-day-old seedlings. Co-expression between A and E V-ATPase subunits was also observed. The hydrolytic and proton transport activities showed that these enzymes presented a differential modulation of their activities in the presence of 100 mM NaCl. These results suggest that V-ATPase and V-PPase activities are modulated by salt stress and a multi-step regulation is exerted in order to re-establish homeostasis
Fil: Menezes Sobreira, Alana Cecília. Universidade Estadual Do Ceara; Brasil. Universidade Federal Do Ceara; Brasil
Fil: Maia, Yuri. Universidade Federal Do Ceara; Brasil
Fil: Rebouças, Deborah Moura. Universidade Federal Do Ceara; Brasil
Fil: Costa, José Hélio. Universidade Federal Do Ceara; Brasil
Fil: Oliveira Otoch, Maria de Lourdes. Universidade Estadual Do Ceara; Brasil
Fil: Nogueira de Oliveira, Luciana Maia. Universidade Federal Rural Pernambuco; Brasil
Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Fernandes de Melo, Dirce. Universidade Federal do Ceará; Brasil
description Global climatic changes as high temperatures and low precipitation contribute to increase cultivated areas affected by high salt soil content. Soil salinity is well known to reduce the ability of plants to take up water and this quickly causes reduction in their growth rate. V-ATPase (EC 3.6.3.14) and V-PPase (EC 3.6.1.1) hydrolytic and proton transport activities, and gene expression were evaluated in hypocotyls of 3-, 5-, 7-day-old Vigna unguiculata (L.) Walp cv. Vita 3 germinated in 100 mM NaCl in order to highlight their differential regulation and activity modulation under salt stress. Semi-quantitative RT-PCR revealed that both genes were up-regulated by salt stress in all salt exposition times studied. Up-regulation was correlated with the increase in protein content at 5 and 7-day-old seedlings. Co-expression between A and E V-ATPase subunits was also observed. The hydrolytic and proton transport activities showed that these enzymes presented a differential modulation of their activities in the presence of 100 mM NaCl. These results suggest that V-ATPase and V-PPase activities are modulated by salt stress and a multi-step regulation is exerted in order to re-establish homeostasis
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29630
Menezes Sobreira, Alana Cecília; Maia, Yuri; Rebouças, Deborah Moura; Costa, José Hélio; Oliveira Otoch, Maria de Lourdes; et al.; Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress; Springer; Theoretical and Experimental Plant Physiology; 26; 5-2014; 167-175
2197-0025
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29630
identifier_str_mv Menezes Sobreira, Alana Cecília; Maia, Yuri; Rebouças, Deborah Moura; Costa, José Hélio; Oliveira Otoch, Maria de Lourdes; et al.; Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress; Springer; Theoretical and Experimental Plant Physiology; 26; 5-2014; 167-175
2197-0025
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s40626-014-0012-4
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs40626-014-0012-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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