Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress
- Autores
- Menezes Sobreira, Alana Cecília; Maia, Yuri; Rebouças, Deborah Moura; Costa, José Hélio; Oliveira Otoch, Maria de Lourdes; Nogueira de Oliveira, Luciana Maia; Orellano, Elena Graciela; Fernandes de Melo, Dirce
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Global climatic changes as high temperatures and low precipitation contribute to increase cultivated areas affected by high salt soil content. Soil salinity is well known to reduce the ability of plants to take up water and this quickly causes reduction in their growth rate. V-ATPase (EC 3.6.3.14) and V-PPase (EC 3.6.1.1) hydrolytic and proton transport activities, and gene expression were evaluated in hypocotyls of 3-, 5-, 7-day-old Vigna unguiculata (L.) Walp cv. Vita 3 germinated in 100 mM NaCl in order to highlight their differential regulation and activity modulation under salt stress. Semi-quantitative RT-PCR revealed that both genes were up-regulated by salt stress in all salt exposition times studied. Up-regulation was correlated with the increase in protein content at 5 and 7-day-old seedlings. Co-expression between A and E V-ATPase subunits was also observed. The hydrolytic and proton transport activities showed that these enzymes presented a differential modulation of their activities in the presence of 100 mM NaCl. These results suggest that V-ATPase and V-PPase activities are modulated by salt stress and a multi-step regulation is exerted in order to re-establish homeostasis
Fil: Menezes Sobreira, Alana Cecília. Universidade Estadual Do Ceara; Brasil. Universidade Federal Do Ceara; Brasil
Fil: Maia, Yuri. Universidade Federal Do Ceara; Brasil
Fil: Rebouças, Deborah Moura. Universidade Federal Do Ceara; Brasil
Fil: Costa, José Hélio. Universidade Federal Do Ceara; Brasil
Fil: Oliveira Otoch, Maria de Lourdes. Universidade Estadual Do Ceara; Brasil
Fil: Nogueira de Oliveira, Luciana Maia. Universidade Federal Rural Pernambuco; Brasil
Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Fernandes de Melo, Dirce. Universidade Federal do Ceará; Brasil - Materia
-
Cowpea
Proton Pump
Salinity Stress
Vacuolar Membrane - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/29630
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stressMenezes Sobreira, Alana CecíliaMaia, YuriRebouças, Deborah MouraCosta, José HélioOliveira Otoch, Maria de LourdesNogueira de Oliveira, Luciana MaiaOrellano, Elena GracielaFernandes de Melo, DirceCowpeaProton PumpSalinity StressVacuolar Membranehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Global climatic changes as high temperatures and low precipitation contribute to increase cultivated areas affected by high salt soil content. Soil salinity is well known to reduce the ability of plants to take up water and this quickly causes reduction in their growth rate. V-ATPase (EC 3.6.3.14) and V-PPase (EC 3.6.1.1) hydrolytic and proton transport activities, and gene expression were evaluated in hypocotyls of 3-, 5-, 7-day-old Vigna unguiculata (L.) Walp cv. Vita 3 germinated in 100 mM NaCl in order to highlight their differential regulation and activity modulation under salt stress. Semi-quantitative RT-PCR revealed that both genes were up-regulated by salt stress in all salt exposition times studied. Up-regulation was correlated with the increase in protein content at 5 and 7-day-old seedlings. Co-expression between A and E V-ATPase subunits was also observed. The hydrolytic and proton transport activities showed that these enzymes presented a differential modulation of their activities in the presence of 100 mM NaCl. These results suggest that V-ATPase and V-PPase activities are modulated by salt stress and a multi-step regulation is exerted in order to re-establish homeostasisFil: Menezes Sobreira, Alana Cecília. Universidade Estadual Do Ceara; Brasil. Universidade Federal Do Ceara; BrasilFil: Maia, Yuri. Universidade Federal Do Ceara; BrasilFil: Rebouças, Deborah Moura. Universidade Federal Do Ceara; BrasilFil: Costa, José Hélio. Universidade Federal Do Ceara; BrasilFil: Oliveira Otoch, Maria de Lourdes. Universidade Estadual Do Ceara; BrasilFil: Nogueira de Oliveira, Luciana Maia. Universidade Federal Rural Pernambuco; BrasilFil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Fernandes de Melo, Dirce. Universidade Federal do Ceará; BrasilSpringer2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29630Menezes Sobreira, Alana Cecília; Maia, Yuri; Rebouças, Deborah Moura; Costa, José Hélio; Oliveira Otoch, Maria de Lourdes; et al.; Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress; Springer; Theoretical and Experimental Plant Physiology; 26; 5-2014; 167-1752197-0025CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s40626-014-0012-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs40626-014-0012-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:30Zoai:ri.conicet.gov.ar:11336/29630instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:31.048CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress |
title |
Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress |
spellingShingle |
Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress Menezes Sobreira, Alana Cecília Cowpea Proton Pump Salinity Stress Vacuolar Membrane |
title_short |
Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress |
title_full |
Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress |
title_fullStr |
Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress |
title_full_unstemmed |
Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress |
title_sort |
Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress |
dc.creator.none.fl_str_mv |
Menezes Sobreira, Alana Cecília Maia, Yuri Rebouças, Deborah Moura Costa, José Hélio Oliveira Otoch, Maria de Lourdes Nogueira de Oliveira, Luciana Maia Orellano, Elena Graciela Fernandes de Melo, Dirce |
author |
Menezes Sobreira, Alana Cecília |
author_facet |
Menezes Sobreira, Alana Cecília Maia, Yuri Rebouças, Deborah Moura Costa, José Hélio Oliveira Otoch, Maria de Lourdes Nogueira de Oliveira, Luciana Maia Orellano, Elena Graciela Fernandes de Melo, Dirce |
author_role |
author |
author2 |
Maia, Yuri Rebouças, Deborah Moura Costa, José Hélio Oliveira Otoch, Maria de Lourdes Nogueira de Oliveira, Luciana Maia Orellano, Elena Graciela Fernandes de Melo, Dirce |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Cowpea Proton Pump Salinity Stress Vacuolar Membrane |
topic |
Cowpea Proton Pump Salinity Stress Vacuolar Membrane |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Global climatic changes as high temperatures and low precipitation contribute to increase cultivated areas affected by high salt soil content. Soil salinity is well known to reduce the ability of plants to take up water and this quickly causes reduction in their growth rate. V-ATPase (EC 3.6.3.14) and V-PPase (EC 3.6.1.1) hydrolytic and proton transport activities, and gene expression were evaluated in hypocotyls of 3-, 5-, 7-day-old Vigna unguiculata (L.) Walp cv. Vita 3 germinated in 100 mM NaCl in order to highlight their differential regulation and activity modulation under salt stress. Semi-quantitative RT-PCR revealed that both genes were up-regulated by salt stress in all salt exposition times studied. Up-regulation was correlated with the increase in protein content at 5 and 7-day-old seedlings. Co-expression between A and E V-ATPase subunits was also observed. The hydrolytic and proton transport activities showed that these enzymes presented a differential modulation of their activities in the presence of 100 mM NaCl. These results suggest that V-ATPase and V-PPase activities are modulated by salt stress and a multi-step regulation is exerted in order to re-establish homeostasis Fil: Menezes Sobreira, Alana Cecília. Universidade Estadual Do Ceara; Brasil. Universidade Federal Do Ceara; Brasil Fil: Maia, Yuri. Universidade Federal Do Ceara; Brasil Fil: Rebouças, Deborah Moura. Universidade Federal Do Ceara; Brasil Fil: Costa, José Hélio. Universidade Federal Do Ceara; Brasil Fil: Oliveira Otoch, Maria de Lourdes. Universidade Estadual Do Ceara; Brasil Fil: Nogueira de Oliveira, Luciana Maia. Universidade Federal Rural Pernambuco; Brasil Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Fernandes de Melo, Dirce. Universidade Federal do Ceará; Brasil |
description |
Global climatic changes as high temperatures and low precipitation contribute to increase cultivated areas affected by high salt soil content. Soil salinity is well known to reduce the ability of plants to take up water and this quickly causes reduction in their growth rate. V-ATPase (EC 3.6.3.14) and V-PPase (EC 3.6.1.1) hydrolytic and proton transport activities, and gene expression were evaluated in hypocotyls of 3-, 5-, 7-day-old Vigna unguiculata (L.) Walp cv. Vita 3 germinated in 100 mM NaCl in order to highlight their differential regulation and activity modulation under salt stress. Semi-quantitative RT-PCR revealed that both genes were up-regulated by salt stress in all salt exposition times studied. Up-regulation was correlated with the increase in protein content at 5 and 7-day-old seedlings. Co-expression between A and E V-ATPase subunits was also observed. The hydrolytic and proton transport activities showed that these enzymes presented a differential modulation of their activities in the presence of 100 mM NaCl. These results suggest that V-ATPase and V-PPase activities are modulated by salt stress and a multi-step regulation is exerted in order to re-establish homeostasis |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/29630 Menezes Sobreira, Alana Cecília; Maia, Yuri; Rebouças, Deborah Moura; Costa, José Hélio; Oliveira Otoch, Maria de Lourdes; et al.; Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress; Springer; Theoretical and Experimental Plant Physiology; 26; 5-2014; 167-175 2197-0025 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/29630 |
identifier_str_mv |
Menezes Sobreira, Alana Cecília; Maia, Yuri; Rebouças, Deborah Moura; Costa, José Hélio; Oliveira Otoch, Maria de Lourdes; et al.; Vacuolar proton pumps regulation during development of Vigna unguiculata seedlings under salt stress; Springer; Theoretical and Experimental Plant Physiology; 26; 5-2014; 167-175 2197-0025 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s40626-014-0012-4 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs40626-014-0012-4 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613935517401088 |
score |
13.070432 |