Peptide synthesis: Chemical or enzymatic

Autores
Guzmán, Fanny; Barberis, Sonia Esther; Illanes, Andrés
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of  the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with   proteolytic   enzymes    is   reviewed    considering  medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic  synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective.
Fil: Guzmán, Fanny. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Barberis, Sonia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Illanes, Andrés. Pontificia Universidad Catolica de Valparaiso. Escuela de Ingeniería Bioquímica; Chile
Materia
PEPTIDES
SOLID-PHASE SYNTHESIS
ENZYMATIC SYNTHESIS
PROTEASES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214003
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Peptide synthesis: Chemical or enzymaticGuzmán, FannyBarberis, Sonia EstherIllanes, AndrésPEPTIDESSOLID-PHASE SYNTHESISENZYMATIC SYNTHESISPROTEASEShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of  the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with   proteolytic   enzymes    is   reviewed    considering  medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic  synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective.Fil: Guzmán, Fanny. Pontificia Universidad Católica de Valparaíso; ChileFil: Barberis, Sonia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Illanes, Andrés. Pontificia Universidad Catolica de Valparaiso. Escuela de Ingeniería Bioquímica; ChilePontificia Universidad Católica de Valparaíso2007-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214003Guzmán, Fanny; Barberis, Sonia Esther; Illanes, Andrés; Peptide synthesis: Chemical or enzymatic; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 10; 2; 4-2007; 279-3140717-3458CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2225/vol10-issue2-fulltext-13info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:51:39Zoai:ri.conicet.gov.ar:11336/214003instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:51:40.045CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Peptide synthesis: Chemical or enzymatic
title Peptide synthesis: Chemical or enzymatic
spellingShingle Peptide synthesis: Chemical or enzymatic
Guzmán, Fanny
PEPTIDES
SOLID-PHASE SYNTHESIS
ENZYMATIC SYNTHESIS
PROTEASES
title_short Peptide synthesis: Chemical or enzymatic
title_full Peptide synthesis: Chemical or enzymatic
title_fullStr Peptide synthesis: Chemical or enzymatic
title_full_unstemmed Peptide synthesis: Chemical or enzymatic
title_sort Peptide synthesis: Chemical or enzymatic
dc.creator.none.fl_str_mv Guzmán, Fanny
Barberis, Sonia Esther
Illanes, Andrés
author Guzmán, Fanny
author_facet Guzmán, Fanny
Barberis, Sonia Esther
Illanes, Andrés
author_role author
author2 Barberis, Sonia Esther
Illanes, Andrés
author2_role author
author
dc.subject.none.fl_str_mv PEPTIDES
SOLID-PHASE SYNTHESIS
ENZYMATIC SYNTHESIS
PROTEASES
topic PEPTIDES
SOLID-PHASE SYNTHESIS
ENZYMATIC SYNTHESIS
PROTEASES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of  the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with   proteolytic   enzymes    is   reviewed    considering  medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic  synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective.
Fil: Guzmán, Fanny. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Barberis, Sonia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Illanes, Andrés. Pontificia Universidad Catolica de Valparaiso. Escuela de Ingeniería Bioquímica; Chile
description Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of  the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with   proteolytic   enzymes    is   reviewed    considering  medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic  synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective.
publishDate 2007
dc.date.none.fl_str_mv 2007-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214003
Guzmán, Fanny; Barberis, Sonia Esther; Illanes, Andrés; Peptide synthesis: Chemical or enzymatic; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 10; 2; 4-2007; 279-314
0717-3458
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214003
identifier_str_mv Guzmán, Fanny; Barberis, Sonia Esther; Illanes, Andrés; Peptide synthesis: Chemical or enzymatic; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 10; 2; 4-2007; 279-314
0717-3458
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2225/vol10-issue2-fulltext-13
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pontificia Universidad Católica de Valparaíso
publisher.none.fl_str_mv Pontificia Universidad Católica de Valparaíso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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