Peptide synthesis: Chemical or enzymatic
- Autores
- Guzmán, Fanny; Barberis, Sonia Esther; Illanes, Andrés
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective.
Fil: Guzmán, Fanny. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Barberis, Sonia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Illanes, Andrés. Pontificia Universidad Catolica de Valparaiso. Escuela de Ingeniería Bioquímica; Chile - Materia
-
PEPTIDES
SOLID-PHASE SYNTHESIS
ENZYMATIC SYNTHESIS
PROTEASES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/214003
Ver los metadatos del registro completo
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Peptide synthesis: Chemical or enzymaticGuzmán, FannyBarberis, Sonia EstherIllanes, AndrésPEPTIDESSOLID-PHASE SYNTHESISENZYMATIC SYNTHESISPROTEASEShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective.Fil: Guzmán, Fanny. Pontificia Universidad Católica de Valparaíso; ChileFil: Barberis, Sonia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Illanes, Andrés. Pontificia Universidad Catolica de Valparaiso. Escuela de Ingeniería Bioquímica; ChilePontificia Universidad Católica de Valparaíso2007-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214003Guzmán, Fanny; Barberis, Sonia Esther; Illanes, Andrés; Peptide synthesis: Chemical or enzymatic; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 10; 2; 4-2007; 279-3140717-3458CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2225/vol10-issue2-fulltext-13info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:57Zoai:ri.conicet.gov.ar:11336/214003instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:57.533CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Peptide synthesis: Chemical or enzymatic |
title |
Peptide synthesis: Chemical or enzymatic |
spellingShingle |
Peptide synthesis: Chemical or enzymatic Guzmán, Fanny PEPTIDES SOLID-PHASE SYNTHESIS ENZYMATIC SYNTHESIS PROTEASES |
title_short |
Peptide synthesis: Chemical or enzymatic |
title_full |
Peptide synthesis: Chemical or enzymatic |
title_fullStr |
Peptide synthesis: Chemical or enzymatic |
title_full_unstemmed |
Peptide synthesis: Chemical or enzymatic |
title_sort |
Peptide synthesis: Chemical or enzymatic |
dc.creator.none.fl_str_mv |
Guzmán, Fanny Barberis, Sonia Esther Illanes, Andrés |
author |
Guzmán, Fanny |
author_facet |
Guzmán, Fanny Barberis, Sonia Esther Illanes, Andrés |
author_role |
author |
author2 |
Barberis, Sonia Esther Illanes, Andrés |
author2_role |
author author |
dc.subject.none.fl_str_mv |
PEPTIDES SOLID-PHASE SYNTHESIS ENZYMATIC SYNTHESIS PROTEASES |
topic |
PEPTIDES SOLID-PHASE SYNTHESIS ENZYMATIC SYNTHESIS PROTEASES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective. Fil: Guzmán, Fanny. Pontificia Universidad Católica de Valparaíso; Chile Fil: Barberis, Sonia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Illanes, Andrés. Pontificia Universidad Catolica de Valparaiso. Escuela de Ingeniería Bioquímica; Chile |
description |
Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/214003 Guzmán, Fanny; Barberis, Sonia Esther; Illanes, Andrés; Peptide synthesis: Chemical or enzymatic; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 10; 2; 4-2007; 279-314 0717-3458 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/214003 |
identifier_str_mv |
Guzmán, Fanny; Barberis, Sonia Esther; Illanes, Andrés; Peptide synthesis: Chemical or enzymatic; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 10; 2; 4-2007; 279-314 0717-3458 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.2225/vol10-issue2-fulltext-13 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pontificia Universidad Católica de Valparaíso |
publisher.none.fl_str_mv |
Pontificia Universidad Católica de Valparaíso |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614156072779776 |
score |
13.070432 |