Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin

Autores
Siano, Alvaro Sebastían; Tonarelli, Georgina Guadalupe; Imaz, María Susana; Perín, Juan Carlos; Ruggeri, María Natalia; Lopez, M.; Santi, Maria Noemi; Zerbini, Elsa Virginia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Granulysin is a human polypeptide produced by cytolytic cells active against a broad range of microbes. Three peptides covering the regions 25-50 (Gr-1 and Gr-2) and 39-62 (Gr-3) of granulysin were synthesized, and their in vitro activity against Mycobacterium tuberculosis was evaluated. The most active peptide was Gr-1C, containing a disulphide bridge, with Minimal Inhibitory Concentration value of 10.1 μM. In concentrations of up to 50 μM, Gr-1 and Gr2 didn't exceed 30% of hemolysis.
Fil: Siano, Alvaro Sebastían. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Imaz, María Susana. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Fil: Perín, Juan Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Ruggeri, María Natalia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Lopez, M.. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Fil: Santi, Maria Noemi. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Zerbini, Elsa Virginia. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Materia
ANTIMICROBIAL PEPTIDES
GRANULYSIN
MYCOBACTERIUM TUBERCULOSIS
SYNTHETIC PEPTIDES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/98388
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/98388
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from GranulysinSiano, Alvaro SebastíanTonarelli, Georgina GuadalupeImaz, María SusanaPerín, Juan CarlosRuggeri, María NataliaLopez, M.Santi, Maria NoemiZerbini, Elsa VirginiaANTIMICROBIAL PEPTIDESGRANULYSINMYCOBACTERIUM TUBERCULOSISSYNTHETIC PEPTIDEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Granulysin is a human polypeptide produced by cytolytic cells active against a broad range of microbes. Three peptides covering the regions 25-50 (Gr-1 and Gr-2) and 39-62 (Gr-3) of granulysin were synthesized, and their in vitro activity against Mycobacterium tuberculosis was evaluated. The most active peptide was Gr-1C, containing a disulphide bridge, with Minimal Inhibitory Concentration value of 10.1 μM. In concentrations of up to 50 μM, Gr-1 and Gr2 didn't exceed 30% of hemolysis.Fil: Siano, Alvaro Sebastían. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; ArgentinaFil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; ArgentinaFil: Imaz, María Susana. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; ArgentinaFil: Perín, Juan Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; ArgentinaFil: Ruggeri, María Natalia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; ArgentinaFil: Lopez, M.. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; ArgentinaFil: Santi, Maria Noemi. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; ArgentinaFil: Zerbini, Elsa Virginia. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; ArgentinaBentham Science Publishers2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98388Siano, Alvaro Sebastían; Tonarelli, Georgina Guadalupe; Imaz, María Susana; Perín, Juan Carlos; Ruggeri, María Natalia; et al.; Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin; Bentham Science Publishers; Protein and Peptide Letters; 17; 4; 4-2010; 517-5210929-8665CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.benthamdirect.org/pages/content.php?PPL/2010/00000017/00000004/0017E.SGMinfo:eu-repo/semantics/altIdentifier/doi/10.2174/092986610790963555info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:30:36Zoai:ri.conicet.gov.ar:11336/98388instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:30:36.725CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin
title Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin
spellingShingle Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin
Siano, Alvaro Sebastían
ANTIMICROBIAL PEPTIDES
GRANULYSIN
MYCOBACTERIUM TUBERCULOSIS
SYNTHETIC PEPTIDES
title_short Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin
title_full Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin
title_fullStr Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin
title_full_unstemmed Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin
title_sort Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin
dc.creator.none.fl_str_mv Siano, Alvaro Sebastían
Tonarelli, Georgina Guadalupe
Imaz, María Susana
Perín, Juan Carlos
Ruggeri, María Natalia
Lopez, M.
Santi, Maria Noemi
Zerbini, Elsa Virginia
author Siano, Alvaro Sebastían
author_facet Siano, Alvaro Sebastían
Tonarelli, Georgina Guadalupe
Imaz, María Susana
Perín, Juan Carlos
Ruggeri, María Natalia
Lopez, M.
Santi, Maria Noemi
Zerbini, Elsa Virginia
author_role author
author2 Tonarelli, Georgina Guadalupe
Imaz, María Susana
Perín, Juan Carlos
Ruggeri, María Natalia
Lopez, M.
Santi, Maria Noemi
Zerbini, Elsa Virginia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL PEPTIDES
GRANULYSIN
MYCOBACTERIUM TUBERCULOSIS
SYNTHETIC PEPTIDES
topic ANTIMICROBIAL PEPTIDES
GRANULYSIN
MYCOBACTERIUM TUBERCULOSIS
SYNTHETIC PEPTIDES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Granulysin is a human polypeptide produced by cytolytic cells active against a broad range of microbes. Three peptides covering the regions 25-50 (Gr-1 and Gr-2) and 39-62 (Gr-3) of granulysin were synthesized, and their in vitro activity against Mycobacterium tuberculosis was evaluated. The most active peptide was Gr-1C, containing a disulphide bridge, with Minimal Inhibitory Concentration value of 10.1 μM. In concentrations of up to 50 μM, Gr-1 and Gr2 didn't exceed 30% of hemolysis.
Fil: Siano, Alvaro Sebastían. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Imaz, María Susana. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Fil: Perín, Juan Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Ruggeri, María Natalia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Lopez, M.. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Fil: Santi, Maria Noemi. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Zerbini, Elsa Virginia. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
description Granulysin is a human polypeptide produced by cytolytic cells active against a broad range of microbes. Three peptides covering the regions 25-50 (Gr-1 and Gr-2) and 39-62 (Gr-3) of granulysin were synthesized, and their in vitro activity against Mycobacterium tuberculosis was evaluated. The most active peptide was Gr-1C, containing a disulphide bridge, with Minimal Inhibitory Concentration value of 10.1 μM. In concentrations of up to 50 μM, Gr-1 and Gr2 didn't exceed 30% of hemolysis.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/98388
Siano, Alvaro Sebastían; Tonarelli, Georgina Guadalupe; Imaz, María Susana; Perín, Juan Carlos; Ruggeri, María Natalia; et al.; Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin; Bentham Science Publishers; Protein and Peptide Letters; 17; 4; 4-2010; 517-521
0929-8665
CONICET Digital
CONICET
url http://hdl.handle.net/11336/98388
identifier_str_mv Siano, Alvaro Sebastían; Tonarelli, Georgina Guadalupe; Imaz, María Susana; Perín, Juan Carlos; Ruggeri, María Natalia; et al.; Bactericidal and Hemolytic Activities of Synthetic Peptides Derived from Granulysin; Bentham Science Publishers; Protein and Peptide Letters; 17; 4; 4-2010; 517-521
0929-8665
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.benthamdirect.org/pages/content.php?PPL/2010/00000017/00000004/0017E.SGM
info:eu-repo/semantics/altIdentifier/doi/10.2174/092986610790963555
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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